氧化苦参碱通过抑制miR-188表达上调磷酸酯酶和Tensin同源物(PTEN)抑制癌症细胞恶性行为

IF 0.1 4区 医学
Xiaobo Wang, Yili Hu, Diandian Chen, Le Cheng, Lili Yu, Quanjun Yang
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引用次数: 0

摘要

氧化苦参碱已被应用于多种癌症的抗癌治疗中。本研究旨在探讨miR-188对乳腺癌(BC)细胞进展的潜在影响及其潜在机制。建立大鼠BC模型后,给予大鼠氧化苦参碱(4 mg/kg、8 mg/kg)、西黄丸(阳性对照)和miR-188模拟物(1 mg/kg),分析肿瘤生长、miR-188、MMP-9、MMP-2和PTEN的表达以及BC细胞行为。氧化苦参碱可显著降低肿瘤发生率和肿瘤体积(p<0.05), 8 mg/kg干预组和阳性对照组的肿瘤抑制率更高(p<0.05)。此外,氧化苦参碱或XH可有效降低细胞的增殖、侵袭和迁移速度。值得注意的是,与4 mg/kg氧化苦参碱相比,8 mg/kg氧化苦参碱和XH对BC细胞的抑制作用更为显著。此外,氧化苦参碱或XH显著下调miR-188、MMP-9和MMP-2,上调PTEN。机械地,PTEN被认为是miR-188的靶标,它们之间具有特异性结合。综上所述,氧化苦参碱通过下调miR-188从而增加PTEN的表达来抑制BC细胞行为。本研究可为BC的治疗提供新的依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oxymatrine Inhibits Malignant Behaviors of Breast Cancer Cells by Inhibiting miR-188 Expression to Up-Regulate Phosphatase and Tensin Homolog (PTEN)
Oxymatrine has been applied to anti-cancer therapies for various cancers. The present study aimed to investigate the potential impact of miR-188 on breast cancer (BC) cell progression and underlying mechanism. After establishment of a rat model of BC, rats were administered with oxymatrine (4 mg/kg, 8 mg/kg), Xihuang pill (XH) (positive control), and miR-188 mimic (1 mg/kg) followed by analysis of tumor growth, the expression of miR-188, MMP-9, MMP-2, and PTEN, and BC cell behaviors. Oxymatrine significantly decreased tumor incidence and reduced tumor mass (p<0.05) with 8 mg/kg intervention group and positive control group exhibiting higher tumor inhibition rate (p<0.05). In addition, oxymatrine or XH effectively reduced cell proliferation, invasion and migration rate. Of note, compared to 4 mg/kg oxymatrine, 8 mg/kg oxymatrine and XH showed more significantly inhibitory effects on BC cells. Moreover, oxymatrine or XH significantly downregulated miR-188, MMP-9, and MMP-2 and upregulated PTEN. Mechanically, PTEN was indicated as the target of miR-188 with specific binding between them. In conclusion, Oxymatrine inhibits BC cell behaviors through down-regulation of miR-188 to increase PTEN expression. This study might provide a new basis for the management of BC.
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