Journal of Biomaterials and Tissue Engineering最新文献_第4页

A Protocol for Large-Scale Isolation of Rat Primary Osteoblasts Suitable for Exosome Extraction 适合外泌体提取的大鼠原代成骨细胞的大规模分离方案

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3299

Yuhao Si, Shaoshuo Li, Yang Guo, Lining Wang, Yong Ma, Heng Yin

{"title":"A Protocol for Large-Scale Isolation of Rat Primary Osteoblasts Suitable for Exosome Extraction","authors":"Yuhao Si, Shaoshuo Li, Yang Guo, Lining Wang, Yong Ma, Heng Yin","doi":"10.1166/jbt.2023.3299","DOIUrl":"https://doi.org/10.1166/jbt.2023.3299","url":null,"abstract":"Objective: This study aims to introduce an optimized method for the large-scale isolation of rat primary osteoblasts, suitable for exosome extraction. Methods: An advancement on the traditional secondary enzyme digestion method, manual bone grinding to clean connective\u0000 tissue and ophthalmic cutting into fragments have been replaced with oscillating vortex rinsing and a 14 cm straight head shear in a centrifuge tube to cut bone fragments, significantly reducing the experimental time. The experiment was conducted on forty suckling rats. The experimental time\u0000 of both the optimized and traditional methods were compared. The osteoblasts obtained through the optimized method were observed morphologically, identified by Alkaline phosphatase and alizarin red staining, and their proliferation activity was detected using the EdU method. Sufficient cell\u0000 supernatant was collected to extract osteoblast-derived exosomes, identified by employing transmission electron microscopy, nanoparticle tracking analysis, and Western blot. Result: The total experimental time of the improved method (5.5±0.17 hours) was significantly shorter\u0000 than the traditional method (7.25±0.23 hours) with a notable statistical difference (P <0.001); the osteoblasts obtained by the improved method displayed good growth status. On the seventh day, the cells matured and exhibited positive ALP staining. By the 21st day, mineralized\u0000 nodules were formed, and alizarin red staining was positive. The EdU results showed that osteoblasts demonstrated healthy growth at both 24 and 48 hours, with a proliferation rate of (96.2%±1.3%) at 48 hours, slightly higher than that of (94.6%±2.8%) at 24 hours, with no significant\u0000 statistical difference (P >0.05); Western blot showed positive results for CD9, CD63, and TSG101, and negative results for Calnexin. Conclusion: The optimized primary osteoblast isolation method can significantly improve experimental efficiency and is suitable for extracting\u0000 osteoblast-derived exosomes.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49097888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nursing Intervention in Oral Mucositis After Methotrexate Chemotherapy in Children with Hematological Tumors 血液肿瘤患儿甲氨蝶呤化疗后口腔黏膜炎的护理干预

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3306

Benying Yu, Lin Du, Wenyan Chen, Banghong Xu

{"title":"Nursing Intervention in Oral Mucositis After Methotrexate Chemotherapy in Children with Hematological Tumors","authors":"Benying Yu, Lin Du, Wenyan Chen, Banghong Xu","doi":"10.1166/jbt.2023.3306","DOIUrl":"https://doi.org/10.1166/jbt.2023.3306","url":null,"abstract":"Objective: To explore the nursing intervention and its clinical effect on oral mucositis after methotrexate (MTX) chemotherapy in children with hematological tumors. Methods: Two-hundred children with hematological tumors admitted to Nanjing Children’s Hospital between\u0000 January 2021 and August 2022 and treated with MTX chemotherapy after admission randomly dividing as control and experimental groups (n = 100 every group). Children given conventional clinical nursing and comprehensive oral care. Oral ulcer, quality of life, and satisfaction of children’s\u0000 parents after intervention were compared between 2 groups. Results: SF-36 score after nursing intervention significantly increasing in experimental group compared with that of control group, with a statistically significant difference (p<0.05). Satisfaction of children’s\u0000 parents significantly improving in experimental group compared with that of control group (p<0.05). Conclusion: Comprehensive oral nursing intervention is effective in preventing and treating oral mucositis, improving oral cleanliness and comfort, and developing good oral\u0000 hygiene habits, which is conducive to clinical rehabilitation in children undergoing MTX chemotherapy for hematological tumors.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41482138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Genetic Polymorphisms Contributing to Coronavirus Disease 2019 Susceptibility: A Bibliometric Analysis via CiteSpace 人类基因多态性对2019冠状病毒病易感性的影响:通过CiteSpace进行的文献计量学分析

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3302

Wu Zuchuang, Pengfei Lv

{"title":"Human Genetic Polymorphisms Contributing to Coronavirus Disease 2019 Susceptibility: A Bibliometric Analysis via CiteSpace","authors":"Wu Zuchuang, Pengfei Lv","doi":"10.1166/jbt.2023.3302","DOIUrl":"https://doi.org/10.1166/jbt.2023.3302","url":null,"abstract":"A growing number of related research papers have been published in recent months about COVID-19 as a global pandemic. Research directions and hot topics can be identified by bibliometric analysis of these publications. Using the Web of Science database, we scanned the global literature\u0000 about COVID-19 from 2020 to 2022.A search using keywords such as “COVID-19” and “genetic polymorphisms” led to relevant publications. A bibliometric analysis of these articles was conducted using CiteSpace. We identified 438 publications on COVID-19 with genetic polymorphisms.\u0000 In terms of citation counts, Nature ranked first with 271 citations. Veronica Tisato, Giuseppe Fiorentino, Naoki Yamamoto, and Ingrid Fricke-Galindo were the most productive authors (each having 4 articles). The most cited organization is Tehran University of Medical Sciences with fourteen\u0000 articles. USA is the most prolific collaborator with 76 articles. The polymorphism in the interleukin-6 gene (IL-6) was first studied in 2020. ACE2 gene polymorphism has been the focus of research since 2021. A major contribution to this field was made by the USA. The relationship of ACE2\u0000 gene G8790A polymorphism with COVID-19 and severity may be future hotspots.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47239823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LUNGCAPS-A Novel Hybrid Capsule Networks and Optimized Learning Framework for the Improved Classification of Lung Tumours 一种新的混合胶囊网络和优化的学习框架，用于改进肺肿瘤分类

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3297

M. Manimegalai, P. Suresh Kumar

{"title":"LUNGCAPS-A Novel Hybrid Capsule Networks and Optimized Learning Framework for the Improved Classification of Lung Tumours","authors":"M. Manimegalai, P. Suresh Kumar","doi":"10.1166/jbt.2023.3297","DOIUrl":"https://doi.org/10.1166/jbt.2023.3297","url":null,"abstract":"The development of the intelligent expert system is required mandatorily today for the clinical analysis and to make the accurate diagnosis for disease treatment. Lung cancer diagnosis requires more thorough investigation than other disease processes since it impacts equally men and\u0000 women with a higher fatality rate. Images from a computer tomography (CT) scan can give more useful information about a lung cancer’s diagnosis. Using CT scan input images, numerous machine learning as well as deep learning techniques are developed for the improvement of the medical\u0000 treatment process. But when it comes to developing a precise and intelligent system, research still has a dark side. This research suggests a brand-new classification model that operates on the principles of optimal learning networks and capsules. Capsule network theory is used into the suggested\u0000 framework to enhance classification maps and consequently lower the likelihood of overfitting issues. Additionally, Whale Optimized Feed Forward Layers (WO FFL) have been used in place of the traditional neural network in the suggested study to get the best classification of malignancies in\u0000 lung CT scan. The suggested framework’s simulation results demonstrate improved F 1-score (99.98%), specificity (99.96%), sensitivity (99.95%), and accuracy (99.99%). Additionally, the suggested framework’s performance was compared to that of other traditional system, and\u0000 several performance metrics indicated that the suggested paradigm outperformed the alternatives.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42534496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of Different Pore Sizes of Bovine Bone Scaffold Material on the Oral Microbiota 牛骨支架材料不同孔径对口腔微生物群的影响

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3304

Fangchen Liu, Rui Ma, Yidan Ma, Geng Li, Yongsheng Lin, Bin Ma, Lin Xu, Jianye Zhou

{"title":"The Influence of Different Pore Sizes of Bovine Bone Scaffold Material on the Oral Microbiota","authors":"Fangchen Liu, Rui Ma, Yidan Ma, Geng Li, Yongsheng Lin, Bin Ma, Lin Xu, Jianye Zhou","doi":"10.1166/jbt.2023.3304","DOIUrl":"https://doi.org/10.1166/jbt.2023.3304","url":null,"abstract":"Bone scaffolds are a kind of bone repair material, but bone scaffold infection is a common and serious clinical problem that has not been well studied in the context of the oral microbiota. We prepared two different bone scaffold materials with different pore sizes from adult and fetal\u0000 bovine cancellous bones and compared them with hydroxyapatite (HA) powder. After incubation, we facilitated the initial colonization by the oral microbiota on the three materials by using the saliva of healthy people. Through high-throughput sequencing and analysis, we found that the different\u0000 pore sizes of the bone scaffold materials had a certain influence on the initial colonization by the oral microbiota, affecting β-diversity and functional profiles. Redundancy analysis (RDA) and cooccurrence analysis indicated that initial microbial colonization by the oral microbiota\u0000 in fetal bone scaffold materials with smaller pore sizes was more likely to cause infection. In addition, there may be some functional bacteria that play an interactive role in early colonization by the oral microflora in bone scaffold materials, such as those of the genera Bacillus\u0000 and Lactobacillus.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42615591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of MicroRNA-182-5p Alleviates Pain in Rats with Lumbar Disc Herniation MicroRNA-182-5p过表达减轻腰椎间盘突出症大鼠疼痛

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3300

Maimaitiaili Niyazi, Jie Dai, Xin Eric Wang, Aikeremujiang Muheremu

{"title":"Overexpression of MicroRNA-182-5p Alleviates Pain in Rats with Lumbar Disc Herniation","authors":"Maimaitiaili Niyazi, Jie Dai, Xin Eric Wang, Aikeremujiang Muheremu","doi":"10.1166/jbt.2023.3300","DOIUrl":"https://doi.org/10.1166/jbt.2023.3300","url":null,"abstract":"Inflammatory factor stimulation secondary to lumbar disc herniation (LDH) is considered to be the main cause of lumbar back and lower extremity pain in patients with LDH. In the meanwhile, microRNAs have been reported to be effective in inhibiting the expression of several inflammatory\u0000 factors. In the current study, we used rat LDH model to explore the feasibility of MSCs overexpressing microRNA-182-5p as a treatment option for LDH. Changes of inflammatory factors and changes of histological properties of dorsal root ganglion were observed to test the efficacy of this treatment\u0000 option for lower back and extremity pain due secondary to LDH. All the rats survived by the end of eight week study period. The expression of TNF-α, IL-1, IL-6 both in dorsal root ganglia and blood serum were significantly lower in the experimental group than the control group\u0000 (P <0.01). Histopathologic examination results showed better preserved tissue structural integrity of nerve ganglion in rats treated with BMSCs overexpressing microRNA-182-5p. Those results indicated that, BMSCs overexpressing microRNA-182-5p can significantly inhibit inflammatory\u0000 reaction after LDH, and may be used as a therapeutic option to alleviate pain in patients with neurogenic pain after LDH.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46413230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Mechanism Study of Single Nucleotide Polymorphism rs3094165 Regulating the Progression of Hepatocellular Carcinoma via Modulating NADH Dehydrogenase Ubiquinone Fe–S Protein-1 单核苷酸多态性rs3094165通过调节NADH脱氢酶泛醌铁硫蛋白-1调节肝癌进展的机制研究

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3294

Ziyang Sun, Qingsong Cui, Yong Yu, Zhenhua Lin, Guang Jin, Hesong Cui, Enyue Yang

{"title":"The Mechanism Study of Single Nucleotide Polymorphism rs3094165 Regulating the Progression of Hepatocellular Carcinoma via Modulating NADH Dehydrogenase Ubiquinone Fe–S Protein-1","authors":"Ziyang Sun, Qingsong Cui, Yong Yu, Zhenhua Lin, Guang Jin, Hesong Cui, Enyue Yang","doi":"10.1166/jbt.2023.3294","DOIUrl":"https://doi.org/10.1166/jbt.2023.3294","url":null,"abstract":"Hepatocellular carcinoma (HCC) is a major contributor to global cancer-related mortality, under-scoring the urgent need to identify effective prognostic markers. Single nucleotide polymorphisms (SNPs) play an increasingly important role in predicting the prognosis of patients with HCC.\u0000 In this study, a cohort of 637 HCC patients was enrolled, and various online databases were comprehensively surveyed to identify SNPs that potentially impact HCC patient survival. The results of the Cox regression analysis demonstrated a significant association between rs3094165 and poor prognosis\u0000 among patients with HCC. Further PSM analysis was also employed to control potential biases, and the results remained consistent. These findings suggest that SNPs can serve as reliable prognostic markers for HCC patients. Then, we conducted functional experiments to explore the molecular mechanism\u0000 and confirmed that rs3094165 potentially regulates the progression of HCC by modulating the expression of the NDUFS1. Taken together, our study provides a comprehensive analysis of SNPs associated with HCC prognosis, and highlights the importance of rs3094165 as potential biomarkers for predicting\u0000 poor prognosis of HCC patients. Our findings also shed light on the influence of rs3094165 on HCC progression by regulating NDUFS1 expression.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48722735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors Influencing Selection of Dental Implants Among Implantologists in Saudi Arabia 沙特阿拉伯种植医师选择种植体的影响因素

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3295

Ahmed A. Alzahrani, R. Kayal, Fatemah A. Tomishan

{"title":"Factors Influencing Selection of Dental Implants Among Implantologists in Saudi Arabia","authors":"Ahmed A. Alzahrani, R. Kayal, Fatemah A. Tomishan","doi":"10.1166/jbt.2023.3295","DOIUrl":"https://doi.org/10.1166/jbt.2023.3295","url":null,"abstract":"Objectives: This study aims to determine the variables influencing Saudi Arabia implantologists’ choice of the implant system. Materials and Methods: 78 dental implantologists in Saudi Arabia were interviewed and asked to complete a questionnaire. The questionnaire\u0000 included age and gender demographic, followed by inquiries on the standards for choosing implant systems and used Likert scales. Results: Gender, place of employment, and specialization comparison between the doctor’s opinion and Fisher’s tests will be analyzed. The post-sale\u0000 service (66.6%), size and prosthesis variety (59%), ease of surgical and prosthetic stages (56.4%), geometry and surface, and kind of connections were the most significant factors. The most relevant element in choosing an implant system was the implant system’s popularity (51.2%), and\u0000 the least relevant aspect was a referral from a dentist (20.5%). One of the insignificant variables in choosing an implant system was the manufacturing country (25.6%). Patient preference (25.3%) is the irrelevant and negligible element influencing the choice of dental implant systems. Conclusions:\u0000 This study indicated that most dentists prefer acid etching and blasting surface treatments.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46907794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodontal Diseases and Salivary Secretion of ABH Blood Antigens in Yemeni Patients 也门患者牙周病和唾液ABH血液抗原分泌

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3307

Jamil M. A. S. Obaid, B. Alkadasi, M. Alahmari, Ahmed A. Alzahrani, T. A. AlDhelai, Shaima A. H. Mohammed, Ebtesam M. M. Al-Gora’ee, S. A. Elayah, Mohammed M. Al Moaleem

{"title":"Periodontal Diseases and Salivary Secretion of ABH Blood Antigens in Yemeni Patients","authors":"Jamil M. A. S. Obaid, B. Alkadasi, M. Alahmari, Ahmed A. Alzahrani, T. A. AlDhelai, Shaima A. H. Mohammed, Ebtesam M. M. Al-Gora’ee, S. A. Elayah, Mohammed M. Al Moaleem","doi":"10.1166/jbt.2023.3307","DOIUrl":"https://doi.org/10.1166/jbt.2023.3307","url":null,"abstract":"This study explored the secretion trait as a risk factor for periodontal disease progression. 162 patients suffering from periodontal disease attended the Outpatient Dental Clinics at the Faculty of Dentistry, Ibb University, and 69 healthy volunteers were enrolled in this study. Clinical\u0000 evaluation for periodontal disease was carried out according to international guidelines and expressed by pocket probing depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL) parameters. A blood sample was collected and tested for blood grouping test, and a saliva sample\u0000 was analyzed for secretion using hemagglutination inhibition test. Secretor patients were 82% compared with 64% of healthy control. Mean score of BOB and PPD and the clinical magnitude of CAL parameters were increased within secretors. The development of periodontal diseases increased among\u0000 secretors by 2.61 times more than non-secretors (OR = 2.61, CI, 1.382–4.914, p = 0.004). Significant periodontal complaints associated with secretion are Bleeding gums (OR = 1.95, CI, 1.011–3.759, p = 0.049), malaligned teeth (OR = 4.49 (CI, 1.842–10.958), p\u0000 = 0.0001), gingival recession (OR = 2.48, CI, 1.322–4.648, p = 0.007), and teeth decay (OR = 1.86, CI, 1.006–3.438, p = 0.05). Secretion of ABH antigens is risk factor for progression of periodontal diseases. The odds of periodontal complaints, bleeding gums, malaligned\u0000 teeth, gingival recession, and tooth decay prove the ABH secretion effect.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49615141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Biomarker Evaluation in Peri-Implant Crevicular Fluid as Disease Indicators for Peri-Implant Health 新型生物标志物评价在种植体周围沟液作为种植体周围健康的疾病指标

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3293

A. Alsahhaf, E. Alhamdan, Fahim Vohra, T. Abduljabbar, Ziaullah Chaudhry, Isaac Kuyunov

{"title":"Novel Biomarker Evaluation in Peri-Implant Crevicular Fluid as Disease Indicators for Peri-Implant Health","authors":"A. Alsahhaf, E. Alhamdan, Fahim Vohra, T. Abduljabbar, Ziaullah Chaudhry, Isaac Kuyunov","doi":"10.1166/jbt.2023.3293","DOIUrl":"https://doi.org/10.1166/jbt.2023.3293","url":null,"abstract":"Objective: To assess various inflammatory biomarkers CCL-20, BAF, RANK-L, IL-23, and osteoprotegerin from PICF along with clinical parameters in participants with peri-implant mucositis and peri-implantitis. Material and Methods: Participants were gathered i.e., 30 with\u0000 periimplantitis, 32 with peri-implant mucositis, and 32 healthy. Peri-implant parameters PIPD, BoP, and PIPI were measured in all participants. PICF samples were collected to assess the level of biomarkers CCL-20; BAF; IL-23; RANK-L, and OPG. For periodontal parameters, ranges and means were\u0000 measured. Kruskal Wallis test was used for comparison between groups. For categorical data sets, the Pearson Chi-Square test was applied. The Bonferroni test was used for multiple comparisons. Results: Peri-implant parameter BoP in peri-implant mucositis and peri-implantitis was significantly\u0000 higher compared to controls (p < 0.05). PIPD with healthy peri-implant conditions was, significantly less compared to peri-implantitis, and peri-implant mucositis (p<0.05). PIPI demonstrated no significant difference throughout different peri-implant conditions. CCL-20\u0000 ng/mL in patients with peri-implant mucositis was found to be significantly higher (p < 0.05). BAF levels in peri-implantitis and peri-implant mucositis were comparable. The concentration of IL-23 ng/mL was found to be significantly lower in healthy controls (p < 0.05).\u0000 Conclusion: Inflammatory biomarkers showed high levels of PICF in peri-implant disease patients compared to healthy controls. Individuals with peri-implant conditions experience the poor peri-implant probing depth and bleeding on probing.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48104462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0