Journal of Biomaterials and Tissue Engineering最新文献_第3页

Low-Level Laser Therapy of Er, Cr: YSGG and Femtosecond on Dentin Adhesion with Bioactive and Resin-Modified Glass Ionomer Cement Er、Cr：YSGG和飞秒激光对生物活性和树脂改性玻璃离子水泥牙本质粘附性的影响

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.3313

Fahad H. Alkhudhairy, Y. AlFawaz

{"title":"Low-Level Laser Therapy of Er, Cr: YSGG and Femtosecond on Dentin Adhesion with Bioactive and Resin-Modified Glass Ionomer Cement","authors":"Fahad H. Alkhudhairy, Y. AlFawaz","doi":"10.1166/jbt.2023.3313","DOIUrl":"https://doi.org/10.1166/jbt.2023.3313","url":null,"abstract":"Aim: This study examined the shear bond strength (SBS) and mode of failure of bioactive resin cement (BARC) and resin-modified glass ionomer cement (RMGIC) to dentin treated with Er, Cr: YSGG (ECrL) and femtosecond laser (FSL). Methods: 120 non-carious, non-fractured human molars without prior restorations were selected and processed. The teeth were grouped by surface conditioning. Groups 1 and 5 were untreated controls. Groups 2 and 6 had ECrL surface treatment, while Groups 3 and 7 had FSL. EDTA and Tetric N-Bond Universal conditioned Groups 4 and 8. Groups 1–4 (n = 15) employed BARC for bonding, while Groups 5–8 used RMGIC. A universal testing machine (UTM) tested shear bond strength, and a stereomicroscope studied the failure mode. Comparing findings required means, SDs, ANOVA, and Tukey’s post hoc test. Results: Group 1, without conditioning, has the lowest BARC-bonded SBS. In Group 4, EDTA+ Tetric N-Bond Universal-conditioned dentin bonded to BARC had the greatest SBS values. In the RMGIC-bonded groups, Group 5 without dentin conditioning had the lowest bond values, while EDTA+ Tetric NBond Universal-conditioned dentin had the greatest. Conclusion: EDTA and Tetric N-Bond Universal dentin conditioning improves RMGIC and BARC bond strength. This study’s conditioning methods boosted bond strength.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of Small Nucleolar RNA Host Gene 14 Alleviates Sepsis-Induced Acute Kidney Injury by Targeting miR-145-5p In Vitro and In Vivo 通过靶向体外和体内 miR-145-5p 敲除小核糖核酸宿主基因 14 减轻败血症诱发的急性肾损伤

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.2627

Gang Chen, Wenjing Wang, Min Zhu, Chuji Cheng

{"title":"Knockdown of Small Nucleolar RNA Host Gene 14 Alleviates Sepsis-Induced Acute Kidney Injury by Targeting miR-145-5p In Vitro and In Vivo","authors":"Gang Chen, Wenjing Wang, Min Zhu, Chuji Cheng","doi":"10.1166/jbt.2023.2627","DOIUrl":"https://doi.org/10.1166/jbt.2023.2627","url":null,"abstract":"Acute kidney injury (AKI) manly occurred after sepsis and led to severe damage of the kidney. The present study was designed to explore the underlying mechanism of long non-coding RNA (lncRNA) SNHG14 in sepsis-induced AKI in In Vitro and In Vivo. The cecal ligation and puncture (CLP) rats was used as sepsis-induced AKI model for In Vivo studies and HK-2 cells with lipopolysaccharide (LPS) administration were used as cell model for In Vitro studies. Kidney tissues were stained by Hematoxylin and Eosin (H&E) for histological examination. The relative levels of SNHG14 and miR-145-5p was detected using qRT-PCR. The levels of serum cytokines, inflammatory factors, oxidative stress factors, apoptosis-related proteins were evaluated by ELISA and western blot. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Compared with the sham group, the rats in the CLP group showed injured pathological morphology of kidney, high levels of serum Scr, and BUN, elevated contents of TNF-α, IL-6 and IL-1β, malondialdehyde (MDA) and apoptosis, but low SOD level in kidney tissues. However, all these changes were reversed by shRNA-SNHG14 administration or transfection In Vivo and In Vitro. SNHG14 level was upregulated, while miR-145-5p significantly decreased in kidney tissues of CLP rats and LPS-induced HK-2 cells. The target gene of miR-145-5p was SNHG14, verifying through luciferase reporter analysis. Further, miR-145-5p knockdown transfection significantly reversed the inhibition of SNHG14 interference on the secretion of inflammatory cytokines and apoptosis in LPS-induced HK-2 cells. SNHG14 silence can alleviate AKI in sepsis by upregulating the miR-145-5p expression. SNHG14 might be used as a potential therapeutic target for sepsis-induced AKI.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"8 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Er, Cr: YSGG, Nd: YVO4, and Ti: Sapphire Lasers for Surface Treatment of Hybrid Ceramics on Bond Durability, Surface Texture, and Debonding to Resin Cement: A Scanning Electron Microscopy (SEM) Analysis Er、Cr：用于混合陶瓷表面处理的铒、铬、YSGG、钕、YVO4 和钛、蓝宝石激光器对粘接耐久性、表面纹理以及与树脂水泥脱粘的影响：扫描电子显微镜 (SEM) 分析

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.3318

M. Alrabiah, Hanan Alsunbul, Basil Almutairi, Mohammad Aljanakh, Khaled M. Alzahrani, T. Abduljabbar

{"title":"Er, Cr: YSGG, Nd: YVO4, and Ti: Sapphire Lasers for Surface Treatment of Hybrid Ceramics on Bond Durability, Surface Texture, and Debonding to Resin Cement: A Scanning Electron Microscopy (SEM) Analysis","authors":"M. Alrabiah, Hanan Alsunbul, Basil Almutairi, Mohammad Aljanakh, Khaled M. Alzahrani, T. Abduljabbar","doi":"10.1166/jbt.2023.3318","DOIUrl":"https://doi.org/10.1166/jbt.2023.3318","url":null,"abstract":"Aims: To assess composite ceramics treated with Er, Cr: YSGG (ECL), Nd: YVO4, and Ti: Sapphire lasers to samples treated with hydrofluoric acid (HF) and silane (S) for shear bond strength (SBS) and surface roughness (Ra). Material and Methods: 60 disc-shaped HRC specimens. Surface conditioning techniques divided HRC-prepared discs into four groups. Samples in Group 1: HRC pretreated with normal 9.5% HF, Group 2: ECL, Group 3: Ti-Sapphire, Group 4: Nd: YVO4. The profilometer measured all ceramic discs’ average surface Ra. Universal testing machines tested the resin-ceramic interface SBS. Surface topography was assessed using SEM. Shear bond scores and Ra were compared using a one-way ANOVA. A Tukey’s post hoc test assessed several group comparisons. Results: Group 2 (Ti Sapphire Laser+S) samples scored highest in Ra and SBS. Group 4 Nd: YVO4+S had the lowest Ra and SBS values. HF 20 sec+S, (Ti Sapphire Laser+S), (ECL+S), and (Nd: YVO4+S) had comparable SBS and Ra values (p>0.05). Conclusion: Laser-conditioned hybrid ceramics Er, Cr: YSGG, Nd: YVO4, and Ti-Sapphire lasers have shear bond strength and surface roughness comparable to 9.6% hydrofluoric acid.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"9 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Osteogenic Properties of β-TCP+HA+GO Composites β-TCP+HA+GO复合材料的制备与成骨特性

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.3310

Xu-Mei Cheng, Rui Ma, Yan-Bing Zhou, Tao Li, Sheng-Nan Lei, Xin Zheng, Hui-Xiao Zhang, Mei-Tian Liu, Jianye Zhou, Zhi-Qiang Li

{"title":"Preparation and Osteogenic Properties of β-TCP+HA+GO Composites","authors":"Xu-Mei Cheng, Rui Ma, Yan-Bing Zhou, Tao Li, Sheng-Nan Lei, Xin Zheng, Hui-Xiao Zhang, Mei-Tian Liu, Jianye Zhou, Zhi-Qiang Li","doi":"10.1166/jbt.2023.3310","DOIUrl":"https://doi.org/10.1166/jbt.2023.3310","url":null,"abstract":"Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic, biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute or repair material.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"4 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Resin Composite and Biodentine Cervical Marginal Elevation on the Marginal Gap of Two Computer-Aided Design/Computer-Aided Manufacture Endocrown Materials: In Vitro Study 两种计算机辅助设计/计算机辅助制造内冠材料的树脂复合体和 Biodentine 颈缘抬高对边缘间隙的影响比较：体外研究

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.3312

Waleed M S Alqahtani, Salah A. Yousuf, Mohamed F. Metwally, Khaled M. Haggag, A. Barakat, Abdelrahman Alghamdi, Roua Mohammed Y. Almadani, Hajer Aiydh Hamed Alsalmi, Adnan Madani Mohammed Khamis

{"title":"Comparison of Resin Composite and Biodentine Cervical Marginal Elevation on the Marginal Gap of Two Computer-Aided Design/Computer-Aided Manufacture Endocrown Materials: In Vitro Study","authors":"Waleed M S Alqahtani, Salah A. Yousuf, Mohamed F. Metwally, Khaled M. Haggag, A. Barakat, Abdelrahman Alghamdi, Roua Mohammed Y. Almadani, Hajer Aiydh Hamed Alsalmi, Adnan Madani Mohammed Khamis","doi":"10.1166/jbt.2023.3312","DOIUrl":"https://doi.org/10.1166/jbt.2023.3312","url":null,"abstract":"Objective: This study is the impact of resin composite and bio dentine cervical marginal elevation on vertical marginal gap using two CAD/CAM endo-crowns. Materials and Methods: 32 molars were employed, separated into two main groups: Group R, which included 16 molar teeth elevated distally with resin composite each; and Group B, which included 16 molar teeth elevated distally with dentine. The prepared teeth were then restored using endo-crown restorations. There were 2 equal subgroups for each main group (Vita MarkII and Lava Ultimate endo-crowns; n = 8). The samples underwent thermocycling (TC) after the restorations were bonded using dual-cure resin cement. A digital microscope was used to measure the vertical marginal gap. Results: A significant difference in the vertical marginal gap following thermocycling in all groups, except for the Resin composite. The polymer-based group demonstrated better marginal adaptation than other groups. The marginal accuracy produced by the marginal elevation approach, which involves placing a composite filling and dentine in the proximal box before the insertion of ceramic indirect restorations, is comparable to those of ceramics implanted in dentine without margin elevation. Conclusion: The margin elevation technique ensures that the margins created with these materials are as precise as those placed directly in dentin.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"10 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Size-Dependent Mechanical Property Measurement of 1,2-dioleoyl-SN-3-phosphocholine Liposome Using Atomic Force Microscopy 利用原子力显微镜测量 1,2-二油酰-SN-3-磷脂酰胆碱脂质体的尺寸依赖性机械性能

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.3315

J. Kwak, J. Kwak, G. Kim

{"title":"Size-Dependent Mechanical Property Measurement of 1,2-dioleoyl-SN-3-phosphocholine Liposome Using Atomic Force Microscopy","authors":"J. Kwak, J. Kwak, G. Kim","doi":"10.1166/jbt.2023.3315","DOIUrl":"https://doi.org/10.1166/jbt.2023.3315","url":null,"abstract":"Most studies have reported on the effects of bending rigidity using giant unilamellar liposomes. However, few studies have been conducted on nanosized liposomes. We studied the properties of DOPC liposomes of various sizes using atomic force microscopy (AFM). Two different topographies (convex and planar) of DOPC liposomes on a mica surface were observed in the tapping mode in the fluid. The topographic change of the liposomes could be attributed to the interaction force between the AFM tip and the liposome. Small DOPC liposomes (below 1 μm) showed a bending modulus (kbend) ranging between 10−18 and 10−20 J. The bending modulus was found to be size-dependent; it decreased as DOPC liposome size increased. In this study, images of unilamellar DOPC liposomes were obtained in the contact mode. The bending moduli of lipid vesicles from 100 to 1300 nm were measured using an AFM force curve. The bending modulus tended to increase as liposome size decreased. From experimental and theoretical perspectives, we showed that the value of the bending modulus is proportional to 1.5 power of the radius of the liposome.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"105 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial Ageing and Disinfection of Caries Affected Dentin on the Bond Integrity and Failure Analysis of Ion-Releasing Restorative Material 龋坏牙本质的人工老化和消毒对离子释放修复材料粘接完整性和失效分析的影响

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-06-01 DOI: 10.1166/jbt.2023.3314

A. AlShamrani, T. Almohareb, K. Al Ahdal, A. Maawadh, L. Al Deeb, A. Alrahlah

{"title":"Artificial Ageing and Disinfection of Caries Affected Dentin on the Bond Integrity and Failure Analysis of Ion-Releasing Restorative Material","authors":"A. AlShamrani, T. Almohareb, K. Al Ahdal, A. Maawadh, L. Al Deeb, A. Alrahlah","doi":"10.1166/jbt.2023.3314","DOIUrl":"https://doi.org/10.1166/jbt.2023.3314","url":null,"abstract":"Aim: This study examines the SBS of resin-modified glass ionomer cement (RMGIC) on caries-affected dentin after using several cavity disinfectants (ICG activated by PDT, Pr, and SDF). Method: 40 CAD based on ICDAS criteria 3 and 4 teeth were chosen. Teeth were randomly divided into four groups (n = 10). Samples in Groups 1–4 were disinfected with chlorhexidine (CHX), indocyanine green (ICG), Propolis (Pr), and 38% silver diamine fluoride (SDF), respectively. Disinfected samples were bound to RMGIC and aged. SBS was evaluated using the universal testing machine. Stereomicroscope was used to assess bond failure. To compare many groups the Tukey’s Honestly Significant Difference (HSD) test was used. ANOVA was used to compare groups using mean values and standard deviations. Results: CHX-disinfected and RMGIC-bonded CAD had the highest bond values. Group 4 SDF-disinfected and RMGIC-bonded specimens had the lowest bond values. Group 1 samples disinfected with CHX and bonded with RMGIC had the highest bond integrity (p < 0.05) compared to other treatments. Conclusion: Chlorhexidine is the best disinfectant for caries-affected dentin before bonding with resin-modified glass ionomer cement.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"14 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Potential of Human Mesenchymal Stem Cells Engineered with Different Concentrations of TNFα and IFNg 不同浓度TNFα和IFNg工程化人骨髓间充质干细胞的治疗潜力

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3305

M. Alsharidah, Mona Elsafadi, Osamah Al Rugaie, Amer Mahmood, Khalid M. Mohany, Khalid A. Al-Regaiey, K. Alyahya, Abdel-Moneim Hafez Abdel-Moneim, A. El Sadik, M. Abumaree

{"title":"Therapeutic Potential of Human Mesenchymal Stem Cells Engineered with Different Concentrations of TNFα and IFNg","authors":"M. Alsharidah, Mona Elsafadi, Osamah Al Rugaie, Amer Mahmood, Khalid M. Mohany, Khalid A. Al-Regaiey, K. Alyahya, Abdel-Moneim Hafez Abdel-Moneim, A. El Sadik, M. Abumaree","doi":"10.1166/jbt.2023.3305","DOIUrl":"https://doi.org/10.1166/jbt.2023.3305","url":null,"abstract":"Aims: In this study, the authors aimed to examine the functional activities (adhesion, proliferation, and migration) of hMSCs under the effects of various concentrations of inflammatory mediators, such as TNFα or IFNg. Methods: HMSCs were exposed to various\u0000 concentrations of the inflammatory mediators TNFα or IFNg, and the effects of TNFα or IFNg on the functional properties of hMSCs were determined using multiple functional assays (adhesion, proliferation, and migration). HMSC expression of genes (cytokine, chemokine,\u0000 and adhesion molecule genes) was analyzed using real-time polymerase chain reaction (RT–PCR). Results: TNFα promoted their proliferation and migration especially at 100 ng/ml, while IFNg increased proliferation only at concentrations of 10 ng/ml and proliferation\u0000 at 50 and 100 ng/ml. Conclusion: This study evaluated the suitability of hMSCs in treating inflammatory diseases, which are characterized by high levels of inflammatory mediators such as TNFα or IFNg, and proved that the treatment of hMSCs with TNFα or IFNg\u0000 at higher concentrations has a protective effect.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49662080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Combined Effects of Material Surface Chemistry and Vitamin D3 on MC3T3-E1 Cells 材料表面化学与维生素D3对MC3T3-E1细胞的联合作用

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3296

Yan Li, Lijie Sun, Lingli Zhou, Yonggang Ma, Mingyuan Liu

{"title":"The Combined Effects of Material Surface Chemistry and Vitamin D3 on MC3T3-E1 Cells","authors":"Yan Li, Lijie Sun, Lingli Zhou, Yonggang Ma, Mingyuan Liu","doi":"10.1166/jbt.2023.3296","DOIUrl":"https://doi.org/10.1166/jbt.2023.3296","url":null,"abstract":"Separate stimulation by vitamin D3 and material surface chemistry has long witnessed and proved remarkable effects on biological behavior of osteoblasts, but combined stimulation effects of these two have not been reported yet. In this project, the surface of hydroxyl, amino\u0000 and methyl groups were constructed by silicon-alkyl self-assembly technology. Then, chemical surfaces were to be integrated with vitamin D3 at different concentrations (10−7 mol/L, 10−8 mol/L, 10−9 mol/L) to stimulate MC3T3-E1 osteoblasts.\u0000 It turned out to be quite successful in establishing the research platform for combined stimulation of material surface chemistry and vitamin D3. Meanwhile, indicators such as osteoblast adhesion spots, cytoskeleton formation, proliferation, and differentiation have been fully examined\u0000 in the process for us to explore general rules and possible mechanism of combined stimulation effects of these two on early responses of osteoblasts. The results suggest that the combined effects of vitamin D3 and material surface chemistry can induce early responses of osteoblasts\u0000 on different material surfaces, with differing response degrees. Combined stimulation of material surface chemistry-vitamin D3 causes adhesion plaque and cytoskeleton differences, leading to different early cell behaviors. These findings will provide a strong theoretical basis for the treatment\u0000 of osteoporosis and the construction of tissue-engineered bone models in vitro.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43995456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Microhardness of Different Ceramic Materials Used for Esthetic Full-Ceramic Veneers 美观全陶瓷贴面用不同陶瓷材料的表面显微硬度

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-05-01 DOI: 10.1166/jbt.2023.3298

Esraa A. Attar, Ayman Aldharrab, Reem Ajaj

引用次数: 0