Journal of Biomaterials and Tissue Engineering最新文献

{"title":"Enhancement of Antioxidant/Anti-Inflammatory Activity, and Acceleration of Wound Healing Through the Combination of Ginger Extract and NAC: A Promising Approach","authors":"Conglai Zhou, K Zhao, XiaoLi You, Yuanzheng Wang, Long Chen, Li Sun","doi":"10.1166/jbt.2023.3343","DOIUrl":"https://doi.org/10.1166/jbt.2023.3343","url":null,"abstract":"In recent years, there has been significant development and application of bioactive materials with antioxidant and anti-inflammatory properties in skin regenerative medicine. In this study, we utilized sodium hyaluronate (HA), which has good biological activity and compatibility, as a carrier for material mixing. Ginger extract (GE), a natural biologically active compound, was combined with N-Acetyl-L-cysteine (NAC) for the treatment of skin wounds. GE was extracted from fresh ginger juice through freeze-drying, which exhibit pharmacological effects such as antioxidant, anti-inflammatory, and antibacterial properties. Additionally, NAC possesses notable pharmacological activities, particularly as an antioxidant and anti-inflammatory agent. By harnessing the natural advantages of GE in combination with NAC for wound healing, remarkable results were observed in terms of antioxidant efficacy, anti-inflammatory effects, promotion of granulation tissue and wound repair processes. These outcomes surpassed those achieved by using the individual components alone. These experimental findings underscore the potential of combining plant-based medicines and chemical drugs. This approach strengthens the synergistic effects of drug efficacy, reduces the use of and side effects associated with chemical drugs, and holds significant research and development potential. Moreover, it promotes the wider adoption and application of traditional Chinese medicine technology.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"13 11","pages":"1072-1081"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-497 Prevents Septic Kidney Injury and Improves Immune Function Through Inhibition of Janus Kinase/Signal Transducers and Activators of Transcription (JAK/STAT) Pathway","authors":"Shuang Jiang, Qun Liang","doi":"10.1166/jbt.2023.3095","DOIUrl":"https://doi.org/10.1166/jbt.2023.3095","url":null,"abstract":"Abnormal expression of miR-497 is related to the progression of septic renal injury. This study aimed to identify the protective effect of miR-497 on septic renal injury and immune function. We established a rat model of septic renal injury with sham-operated group and treated rats with culture solution of miR-497, gentianella acuta (positive control group), miR-497 plus JAK/STAT pathway agonist, and distilled water (model group). After treatment, urine output and renal histopathological changes were detected. Flow cytometry and RT-qPCR determined the levels of serum Scr, BUN, KIM-1, NGAL, IgG, IgA, and IgM, and evaluated the CD4 + T, CD8 + T, NK cell activity. Western blot assessed the activity of JAK/STAT signaling pathway. The model group and pathway agonist group had the highest 24-hour urine output, serum Scr, BUN, KIM-1, and NGAL levels, followed by miR-497 group and positive control group, and sham-operated group. The CD4 + T, and NK cell activity was reduced with a drop in IgG, IgA, IgM levels in rats with septic renal injury, but treatment with miR-497 or gentianella acuta restored the cell activity and Ig levels and addition of JAK/STAT pathway agonist would further decrease the immune cell activity. There was no difference between the model group and pathway agonist group, miR-497 group and positive control group ( p < 0.05). Following miR-497 group, and sham-operated group, the expression of miR-497 in model group, pathway agonist group, and positive control group was lowest ( p < 0.05). As the expression of JAK and STAT did not vary among five groups ( p > 0.05), we found highest expression of p-JAK and p-STAT levels in model group, positive control group, and pathway agonist group, and lowest expression in sham-operated group. miR-497 inhibits the activity of JAK/STAT signaling, up-regulates the activity of CD4 + T, NK cells and levels of IgG, IgA, IgM, while inhibiting CD8 + T cells activity to improve immune function. In conclusion, miR-497 attenuates septic kidney injury through inhibiting KIM-1 and NGAL expression, indicating that miR-497 and JAK/STAT pathways may be potential therapeutic targets for treating septic kidney injury.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"13 11","pages":"1094-1100"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The m⁶A-Mediated LINC00857 Upregulation Facilitates Glioma Migration, Invasion and Vasculogenic Mimicry Formation","authors":"Bing Guo, Gaopeng Cheng, Guang Chen","doi":"10.1166/jbt.2023.3253","DOIUrl":"https://doi.org/10.1166/jbt.2023.3253","url":null,"abstract":"LINC00857 and m 6 A methylation were validated to be clearly elevated in glioma patient tumor samples and cell lines. We found that silenced the LINC00857 gene repressed vasculogenic mimicry (VM) formation and VM-linked protein expression in glioma cells, and depressed three processes closely implicated with VM formation—migration, invasion, and epithelial-mesenchymal transition (EMT). Meanwhile, VM was also restrained by silencing of LINC00857. To figure out LINC00857's latent functions in glioma and its molecular mechanism, it was further testified that miR-202-3p was LINC00857's target through biological prediction website, the luciferase activity and RNA immunoprecipitation (RIP) assay. Furthermore, elevation of miR-202-3p caused downregulation of HMGA2, which is recognized as miR-202-3p's downstream target. In conclusion, this study clarified m 6 A mediated the upregulation of LINC00857, and enhanced VM formation in glioma cells by negatively regulating miR-202-3p and motivating HMGA2. LINC00857 may be a new therapeutic target for VM formation in glioma.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"13 10","pages":"968-977"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Enamel Demineralization Following the Use of Different Orthodontic Bracket Materials, Etchants, and Adhesive Systems: An In Vitro Study","authors":"Rahaf Zawawi, Naif A. Almosa","doi":"10.1166/jbt.2023.3322","DOIUrl":"https://doi.org/10.1166/jbt.2023.3322","url":null,"abstract":"The aim of this study was to assess enamel demineralization depth following the use of different bracket materials, etchants, and adhesive systems. Ninety-nine extracted human premolars were assigned to nine groups (eight experimental and one control), each including 11 specimens. The experimental groups were bonded to either metal or ceramic brackets and prepared using a total-or self-etchant with a pre-coated or flash-free adhesive system. All specimens were immersed in an artificial demineralization solution for 7 days at pH 4.5 and 37 °C. The brackets were debonded, and the specimens were sectioned buccolingually at the right and left sides of the bracket base margins. Scanning electron microscopy was used to assess the demineralization depth using ImageJ software. Significantly deeper enamel demineralization was observed in all bonded teeth compared to non-bonded teeth (p < 0.001). The adhesive system had no significant effect on the demineralization depth. Metal brackets caused significantly deeper enamel demineralization than ceramic brackets (p <0.005). The use of the total-etch surface treatment caused deeper demineralization compared to that of the self-etch treatment. In conclusion, bonded teeth exhibit deeper enamel demineralization than non-bonded teeth. Metal brackets contribute to deeper demineralization than ceramic brackets. The total-etch surface treatment caused deeper demineralization than self-etch when combined with metal brackets and a pre-coated adhesive system.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"93 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139365483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimensional Accuracy of 3D-Printed, Digital and Conventional Stone Dental Cast of Dentate Patients Using Arch and Teeth Measurements","authors":"K. Al-Aali, Abeer R. Alshehri, Hiba R. Talic, Ayaan A. Magan, Felwa K. Alhomody","doi":"10.1166/jbt.2023.3316","DOIUrl":"https://doi.org/10.1166/jbt.2023.3316","url":null,"abstract":"Aim: This study aimed to compare the conventional, digital, and three-dimensional (3D)-printed casts in terms of their accuracy in quantifying arch and teeth measurements. Materials and Methods: The conventional casts were prepared using polyether dental impressions. Digital impressions of the typodont reference casts were accomplished using an intra-oral scanner. The digital casts were first converted to stereolithography (STL) files which were then edited, and printed using an SLA printer and photo-polymer resin material. The measurements of the first two groups were completed using a digital caliper, while an imaging software was used to measure digital casts. The occlusocervial (OC) height and mesiodistal (MD) width of canines, second premolars (PMs), and molars of each jaw were measured. Additionally, intercanine width (ICW) and intermolar width (IMW) were also measured. Results: Overall, the lowest OC measurement errors were observed for 3D-printed casts for the upper canine, lower canine, and lower molar (0.003±0.01 mm). Similarly, the lowest MD measurement errors were observed for 3D-printed casts for upper canine, upper PM, and lower PM (0.002±0.01 mm). The ICW and IMW measurement errors for the 3D-printed casts were significantly lower (p < 0.01) for the upper ICW (0.011±0.01 mm) and lower IMW (0.017±0.01 mm) than the other two groups. Compared to the conventional and digital casts, the lower ICW (0.013±0.01 mm) and upper IMW (0.017±0.01 mm) measurement errors observed for the 3D-printed casts were also lower, but non-significantly (p > 0.01). Conclusions: 3D-printed casts presented the lowest OC, MD, ICW, and IMW measurement errors, than the conventional and digital casts. The highest measurement errors were associated with the digital casts.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"2 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139365912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Er.Cr.YSGG Laser, Ti Sapphire Laser and Zinc Pthalocyanine (TM ZnPc) Apical Root Canal Treatment on Smear Layer Removal. A Scanning Electron Microscopy Study","authors":"M. Alkahtany, K. Almadi","doi":"10.1166/jbt.2023.3321","DOIUrl":"https://doi.org/10.1166/jbt.2023.3321","url":null,"abstract":"Aims: Comparison of the smear layer-removing capacity of several final irrigants (Er, Cr: YSGG laser (ECL), zinc phthalocyanine (TM-ZnPc), titanium: sapphire laser (Ti)) employed in the apical third of radicular dentin to and ethylenediamine tetraacetic acid (EDTA). Material and Methods: The chamber was opened, and 15 K file established canal patency. TruNatomy prime shaping files treated root canals. Root canal disinfectant was 3 mL of 2.25 NaOCl between each file. After disinfection, the teeth were randomly divided into four groups (n = 10). Groups 1–4:17% EDTA, ECL, Ti: sapphire laser, and TM-ZnPc. The root was separated by chiseling longitudinal grooves on the mesial and distal surfaces. SEM magnification at 5000X was used to evaluate smear layer removal based on dentinal tubule patency in the apical one-third of root canals using Gutman et al. grading system. IBM-SPSS 23.0 analyzed data. One-way ANOVA and Tukey’s post hoc test compared groups. p =0.05. Results: Group-2 (NaOCl+ECL) specimens removed the most smear layer. Group-4 (NaOCl+TM-ZnPc) removed the least smear layer. Group-1 (NaOCl+EDTA), Group-2, and Group-3 (NaOCl+Ti sapphire laser) showed equivalent smear layer removal efficacy. Conclusion: Er: Cr, YSGG, and Ti: sapphire laser with sodium hypochlorite has the potential to remove the apical third smear layer.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"32 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Culture of Human Bone Marrow Mesenchymal Stem Cells by an Improved Method","authors":"Hua Xin, Guang-Hao Tian, Nian-Ping Zhang, Hong-Jie Zhou, Guo-Zong Wang","doi":"10.1166/jbt.2023.3308","DOIUrl":"https://doi.org/10.1166/jbt.2023.3308","url":null,"abstract":"The harvest of BMSCs has been relying on primary culture because construction of mesenchymal stem cell lines is difficult. The whole bone marrow method is a popular method to isolate and culture BMSCs. But the cell purity and extraction efficiency are not satisfactory using the traditional extraction method. The feasibility of an improved whole bone marrow method for extraction of human BMSCs was explored in our study. After multiple rounds of passage and culture, the cells maintained good morphology and proliferation. The characteristics of human BMSCs were demonstrated via in vitro trilineage-induced differentiation experiment and flow cytometry analysis. In conclusion, human BMSCs can be extracted and cultured successfully and simply using the improved whole bone marrow method.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"289 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139366174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Clinical Efficacy of Arthroscopy with Limited Incision in the Treatment of Knee Dislocation with Multiple Ligament Injuries","authors":"Yuqiang Huang, Wanan Qin, Baoqing Tang, Gejin Wei","doi":"10.1166/jbt.2023.3324","DOIUrl":"https://doi.org/10.1166/jbt.2023.3324","url":null,"abstract":"This study retrospectively analyzes the long-term clinical efficacy of single-stage arthroscopy-assisted surgery with limited incision in the treatment of knee dislocation (KD) with multiple ligament injuries (MLI). A total of 19 patients diagnosed with MLI at the First Affiliated Hospital of Guangxi Medical University and the 923th Hospital of the Joint Logistics Support Force of the People’s Liberation Army of China from March 2013 to May 2015 were included in this study. The functional levels were assessed using the Lysholm Knee Scoring Scale, International Knee Documentation Committee (IKDC) Subjective Knee Evaluation Form, Tegner Activity Score, and range of knee motion preoperatively. Non-parametric and Friedman tests were employed to analyze the surgical outcomes. The Lysholm scores increased from a preoperative value of 43.00 (95% confidence interval: 31.00, 50.00) to 88.00 (82.00, 95.00) at the post-operative follow-up of 10–36 months, and further improved to 90.00 (78.00, 98.00) after 8-year follow-up period. The IKDC scores increased from a preoperative value of 32.20 (28.70, 35.60) to 80.50 (60.90, 86.20) at 10–36 months post-operatively and further improved to 86.90 (69.00, 93.10) after 8-year follow-up period. The Tegner scores showed improvement from a preoperative value of 0.00 (0.00, 1.00) to 5.00 (3.00, 5.00) at 10–36 months postoperatively and to 4.0 (3.00, 5.00) after 8-year follow-up period. The ranges of active knee motion increased from preoperative 35.00 (30.00, 40.00) to 130.00 (120.00, 135.00) at 10–36 months and 8 years postoperative follow-up. In conclusion, it is recommended to adopt arthroscopy with limited incision for restoring knee joint stability and motor function.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"13 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining Vicker Hardness and Wear Volume Minimization in Composite Resin with Different Filler Dimensions via Scanning Topography","authors":"Fahad H. Alkhudhairy, Y. AlFawaz, M. Bin-Shuwaish","doi":"10.1166/jbt.2023.3317","DOIUrl":"https://doi.org/10.1166/jbt.2023.3317","url":null,"abstract":"Aim: To compare the surface hardness of the nanohybrid resin composite with the other filler-containing resin composites. Material and Method: Abrasive wear properties of four types of resin composites: Harmonize (HM), Tetric N-Ceram (TNC), Filtek Z250 (Z250), and Filtek Z350 (Z350). Each composite had six 10 mm-diameter, 6 mm-thick cylindrical specimens for testing. The wear test involved subjecting the specimens to 800 cycles of abrasive wear under a 17 kg load. Surface hardness and weight loss were measured using a Vickers indenter micro-hardness tester after polishing. Surface morphology investigation was carried out using SEM on three specimens from each composite after the wear test. To identify significant differences in the wear characteristics of the resin composites, statistical analysis was conducted using one-way analysis of variance (ANOVA) and the Tukey HSD test (with α = 0.05). Results: The highest volumetric wear loss was observed in the Z350 XT nano-filled resin composite (71.19±2.49 mm3). The lowest was found in Z250 micro hybrid restorative material (36.33±1.57 mm3). The highest Vickers hardness was found in the Z250 micro-hybrid restorative material (111.24±2.1). Moreover, the lowest microhardness was found in tetric N ceram (TNC) (58.41±1.22). Conclusion: The wear resistance of micro-hybrid resin composite Z250 was found to be superior to that of other composite resins tested in the study.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"110 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139366096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phycocyanin-Incorporated Zirconium Dioxide Nanoparticles Activated by Photodynamic Therapy for Caries Affected Dentin Disinfection: “An Examination of Adhesive Bond Strength And Scanning Elemental Analysis”","authors":"A. Maawadh, T. Almohareb, K. Al Ahdal, A. AlShamrani, L. Al Deeb, A. Alrahlah","doi":"10.1166/jbt.2023.3320","DOIUrl":"https://doi.org/10.1166/jbt.2023.3320","url":null,"abstract":"Aim: The purpose of the present study was to examine the effect of various disinfection regimes on the antimicrobial efficacy and bond integrity of CAD against two varieties of bacteria: lacto-bacilli and S. mutans. Material and methods: The investigation used SBS, SEM, elemental analysis, disinfection procedures, and failure mode analysis. Biofilm was grown on CAD samples. The specimens were randomly separated into five groups for disinfection: Group 1 received CHX treatment, Group 2 (Phycocyanain) PC treatment, Group 3 PC-PDT, Group 4 PC@ZrO2 NP-PDT, and Group 5 CO2 therapy. After disinfection, CFUs were measured, specimens were reconstituted, and SBS was tested using a universal testing machine (UTM). Stereomicroscope analyzed failures. Kruskal-Walis tested group survival rates. ANOVA and Post Hoc Tukey were used to calculate bond value mean and standard deviation for different disinfection techniques. All analyses used p<0.05. Results: The highest survival rate of S. mutans and Lactobacillus was found in group 2 CAD disinfected with PC only with no activation by PDT. The lowest survival rate of both bacteria was seen in Group 4: PC@ZrO2NP-PDT. Conclusion: PC@ZnO2NP-PDT demonstrated robust antibacterial activity against lactobacillus and S. mutans, albeit with lower bond values when compared to CHX and CO2.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"34 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139364756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}