Journal of Applied Poultry Research最新文献

{"title":"Study on the effects of anthocyanins on the growth performance, immunity, and meat quality of ZI geese","authors":"He Huang ,&nbsp;Size Wang ,&nbsp;Chuicheng Zeng ,&nbsp;Zhengyu Zang ,&nbsp;Yue Pan ,&nbsp;Yuanliang Zhang ,&nbsp;Shan Yue ,&nbsp;JunYing Wang ,&nbsp;Peng Zheng ,&nbsp;Xiuhua Zhao","doi":"10.1016/j.japr.2025.100562","DOIUrl":"10.1016/j.japr.2025.100562","url":null,"abstract":"<div><div>Zi goose is an excellent local breed with fast growth, strong disease resistance, and good meat quality in China. Anthocyanins are natural water-soluble pigments with various biological functions such as promoting growth, antioxidation, and immune regulation. However, research on the beneficial effects of anthocyanins on geese is not yet in-depth. The aim of this study is to investigate the effects of anthocyanins on the growth performance, immunity, and meat quality of Zi geese, providing reference for healthy culture of geese. Two hundred 28-day-old healthy geese were randomly divided into 4 groups with 5 replicates in each group and 10 geese per replicate. Anthocyanins (0, 50, 100, and 200 mg/kg) were added to the goose dietary of the control group (CG), the anthocyanin group 1 (AG1), anthocyanin group 2 (AG2), and anthocyanin group 3 (AG3), respectively. The experimental period was 10 weeks. The results showed that anthocyanin decreased the levels of the interleukin-6 and tumor necrosis factor-α, the abdominal fat percentage, the shear force and drip loss of breast muscle, and increased the levels of immunoglobulin M and interleukin-10, the number of blood lymphocytes, the pH_24h value and a* value of breast muscle. In conclusion, anthocyanins can improve immunity, and meat quality, while reduce abdominal fat percentage in Zi geese. The optimum additive amount of anthocyanin was 100 mg/kg in Zi goose.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100562"},"PeriodicalIF":1.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different feeding strategies on the performance and meat quality of pigeon squabs (Columba livia) in the late stage of growth","authors":"T.W. Liu ,&nbsp;J.Y. Chen ,&nbsp;J.G. Zhu ,&nbsp;L.X. Wang ,&nbsp;X.M. Meng ,&nbsp;H.X. Huan ,&nbsp;Y. Liu ,&nbsp;P. Xie","doi":"10.1016/j.japr.2025.100561","DOIUrl":"10.1016/j.japr.2025.100561","url":null,"abstract":"<div><div>The current study investigated the effects of two different feeding strategies on the performance and meat quality of squabs (White King pigeon). A total of 108, 21-day-old squabs were randomly divided into three artificial crop milk treatments with different crude protein (<strong>CP</strong>) levels (14%, 15%, 16%). The parent-fed squab control treatment consisted of 36 pairs of pigeons caring for 2 squabs. Results showed that the 28-day body weight, average body weight gain, index of abdominal fat and breast muscle, muscle shearing force, and fiber cross-sectional area and diameter of squabs in group 1 (16% CP) were the highest. In addition, the low dripping and freezing losses and the minimum malondialdehyde content of muscles in group 1 (16 % CP) were observed. Meanwhile, the activity of glutathione peroxidase in the muscle of the control group was the lowest. The values of redness and yellowness of muscles were higher in the control group than those in the artificial feeding treatments. In conclusion, although the muscle color of artificially fed pigeons is not as good as that of naturally fed ones, the 16% CP artificial diet significantly promoted the growth performance and muscle antioxidant capacity of squabs. Therefore, using 16% CP level of diet for artificially feeding squabs during the late stage of growth is recommended.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100561"},"PeriodicalIF":1.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A producer survey on implementation of precision livestock farming in the broiler industry","authors":"Terrilyn Klingberg ,&nbsp;Robert Burns ,&nbsp;Shawn Hawkins ,&nbsp;Yang Zhao ,&nbsp;Tanner Thornton ,&nbsp;Susan Schexnayder","doi":"10.1016/j.japr.2025.100548","DOIUrl":"10.1016/j.japr.2025.100548","url":null,"abstract":"<div><div>While Precision Livestock Farming (PLF) offers many potential benefits for broiler producers, the adoption of PLF on U.S. broiler farms has been limited. Determining the status of PLF implementation in the U.S. broiler industry can aid researchers in developing technology better suited to producer needs to potentially increase PLF field adoption. A survey was sent to 531 broiler producers in Tennessee, U.S. This survey was designed to gather detailed information about current PLF implementation on broiler farms, producer interest in specific PLF technology types, and their willingness to adopt PLF into their production operations. The survey included questions about the current use of fifteen specific PLF tools, the importance of real-time data in their operation, planned use of PLF tools, their goals for using PLF, their willingness to participate in a PLF cost-share program, and their trust in data security. This survey identified a lack of familiarity with PLF among broiler producers, but that they would be willing to adopt PLF if it proved to increase farm productivity and profitability.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100548"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of varying levels of benzoic acid on growth performance, haemato-biochemical indices, carcass traits, gut morphology and intestinal microbiota of broiler chickens","authors":"Mercy C. Ogwuegbu ,&nbsp;Prosper Chukwudi ,&nbsp;Chukwuebuka Okafor-Paul ,&nbsp;Henry O. Edeh ,&nbsp;Augustine O. Ani ,&nbsp;Uchele Okpanachi ,&nbsp;Doctor M.N. Mthiyane","doi":"10.1016/j.japr.2025.100556","DOIUrl":"10.1016/j.japr.2025.100556","url":null,"abstract":"<div><div>This study evaluated the effects of varying dietary levels of benzoic acid on the growth performance, haemato-biochemical indices, carcass traits, gut morphology, and intestinal microbiota of broiler chickens. A total of 300 day-old male Arbor acre broiler chicks were assigned to five dietary treatments supplemented with 0, 2.5, 5.0. 7.5, and 10.0 g/kg benzoic acid in a completely randomized design for 42 days. Results indicated that dietary inclusion of benzoic acid significantly influenced the growth performance, with the highest body weight gain (BWG) and improved feed conversion ratio (FCR) observed at 5.0 g/kg inclusion level (<em>P</em> &lt; 0.001). Haemtao-biochemical indices showed that total protein and globulin levels were significantly higher (<em>P</em> &lt; 0.05) at 7.5 and 10.0g/kg, whereas cholesterol, glucose, and uric acid levels decreased with increasing benzoic acid inclusion (<em>P</em> &lt; 0.05). Carcass traits significantly improved at 5.0 g/kg, with higher breast and thigh muscle yields (<em>P</em> &lt; 0.05). Gut morphology was significantly enhanced, with increased villus length, crypt depth, and epithelium thickness at 5.0 and 7.5 g/kg (<em>P</em> &lt; 0.05). The intestinal microbial analysis revealed a dose-dependent effect, <em>Lactobacillus</em> and B<em>ifidobacteria</em> peaked at 7.5 g/kg, while S<em>almonella</em> decreased progressively with increasing benzoic acid levels (<em>P</em> &lt; 0.001). In conclusion, benzoic acid supplementation at 5.0 g/kg optimally enhances broiler performance, gut health, and microbial balance, whereas higher inclusion levels may induce metabolic adaptations or stress responses.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100556"},"PeriodicalIF":1.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation of glycerin on growth performance, meat quality, and blood biochemistry of growing Japanese quails (Coturnix coturnix japonica)","authors":"Z. Li ,&nbsp;M.T. Khan ,&nbsp;M. Adnan ,&nbsp;M. Usman ,&nbsp;U. Farooq ,&nbsp;F. Ali ,&nbsp;M.F. Khalid ,&nbsp;M. Aoun ,&nbsp;U. Mahmood ,&nbsp;B. Siddique ,&nbsp;E. Bughio ,&nbsp;R. Mustafa ,&nbsp;I. Ahmed ,&nbsp;A.A. El-Mansi ,&nbsp;A.A. Gadallah","doi":"10.1016/j.japr.2025.100555","DOIUrl":"10.1016/j.japr.2025.100555","url":null,"abstract":"<div><div>Crude glycerin (CG), a by-product of biodiesel production, has demonstrated potential as an energy source and could serve as a novel ingredient in poultry diets. This study aimed to evaluate the impact of CG supplementation on growth performance, carcass traits, meat quality, and blood parameters in Japanese quails. A total of 375 one-day-old quails were randomly assigned to five dietary treatments in a completely randomized design (CRD). Each treatment was replicated five times, with 15 birds per replicate. The experimental diets included varying levels of CG (0 %, 3 %, 6 %, 9 %, and 12 %). Glycerin was incorporated into the basal diet. Parameters such as feed intake, weight gain, and feed conversion ratio were assessed to gauge the impact of CG on growth performance. Additionally, carcass traits (e.g., carcass yield, breast yield, thigh yield, liver weight, gizzard weight, and heart weight) and meat quality indicators (e.g., thawing loss and drip loss) were analyzed to determine the influence of CG on meat production and quality. The potential effect of CG on the serum biochemical parameters (e.g., serum total protein, albumin, globulin, glucose, cholesterol, triglycerides, and uric acid) was also measured to assess the health and metabolic status of the birds. Data were statistically evaluated using one-way ANOVA under CRD, and treatment means were compared using Duncan's multiple range test at a 5 % significance level. The results of this study demonstrated that the dietary inclusion of 12 % CG significantly enhanced growth performance, as evidenced by increased feed intake, weight gain, and improved feed conversion ratio compared to the lower glycerin levels and control group. Birds fed 12 % CG also had higher carcass yield (P &lt; 0.05), though other carcass traits, such as breast yield, thigh yield, liver weight, gizzard weight, or heart weight, remained unaffected. Meat quality analysis revealed that the inclusion of 12 % CG reduced thawing loss without significantly affecting drip loss. Blood profile analysis showed that the control group had significantly higher serum total protein levels than the 9 % glycerin group, while serum glucose levels were higher in the control group compared to the 12 % CG group. Other parameters, including serum albumin, globulin, cholesterol, triglycerides, and uric acid, were not significantly affected by dietary treatments. In conclusion, 12 % CG supplementation enhanced growth performance, carcass yield, and some meat quality traits without adversely affecting blood profile indicators.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100555"},"PeriodicalIF":1.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A horizontal mixer and a batch-to-horizontal mixer system increased the mix uniformity of free methionine in complete diets relative to a vertical mixer","authors":"L.E. Knarr ,&nbsp;K.M. Bowen ,&nbsp;E.B. Estanich ,&nbsp;J.S. Moritz","doi":"10.1016/j.japr.2025.100554","DOIUrl":"10.1016/j.japr.2025.100554","url":null,"abstract":"<div><div>The coefficient of variation (CV) of a selected nutrient marker is commonly utilized to measure mix uniformity (MU) of a batch of feed, where a CV &lt; 10 % is considered industry-acceptable. Mixer type and mix time (MTI) have been shown to affect mix uniformity (MU), however, data concerning mixing systems (MSY) are unavailable. Additionally, past research has shown that the selection of an appropriate CV marker is integral in properly evaluating MU. Two experiments were conducted to evaluate a vertical, horizontal, and transfer MSY on MU while utilizing inadequate or adequate MTIs for a corn-salt mixture (CSM) and complete diet (CD). Chloride ion (Cl⁻) was selected as the marker for the CSM, and crystalline DL-Methionine (Free Met) and crystalline l-Lysine-HCl (Free Lys) were selected as the markers for the CD. Increasing MTI decreased Cl⁻ CV in the CSM (<em>P</em> = 0.020). An interaction between MSY and MTI affected Free Met CV in the CD (<em>P</em> = 0.007). The horizontal and transfer MSYs did not demonstrate changes in the Free Met CV when MTI was manipulated, whereas the vertical MSY provided decreased CV with increased MTI. The authors hypothesize that additional mixing during load-out and conveyance caused this interaction. However, no treatment provided a Free Lys CV &lt; 10 %. These data suggest that Cl⁻ and Free Met are appropriate markers for MU in CSMs and CDs respectively, transfer MSYs may improve the overall efficiency of feed manufacture, and evaluating MSYs as a whole may provide more representative evaluations of feed before animal consumption.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100554"},"PeriodicalIF":1.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth, carcass characteristics, meat quality, blood indices and gut microbiota of broiler chickens fed diets enriched with a combination of organic Zn, Se and Si nanoparticles","authors":"Elwy A. Ashour ,&nbsp;Ahmed K. Aldhalmi ,&nbsp;Mahmoud Kamal ,&nbsp;Azza R.F. El dosokey ,&nbsp;Ayman A. Swelum","doi":"10.1016/j.japr.2025.100552","DOIUrl":"10.1016/j.japr.2025.100552","url":null,"abstract":"<div><div>This research studied the impacts of feeding different amounts of ZnNPs, SeNPs, and SiNPs on the carcass, gut microbiota, meat quality, growth performance, and blood markers in broilers up to 38 days old. For a total of 300 chicks, five groups were given six replications of ten unsexed one-week broiler chicks (Cobb). At various doses, the five experimental treatment groups received corn-soybean meal-based nutritional supplements: 0.0, 1.5 mg/kg SeNPs + 2.0 mg/kg ZnNPs, 1.5 mg/kg SeNPs + 3.0 mg/kg SiNPs, 2.0 mg/kg ZnNPs + 3.0 mg/kg SiNPs, and 1.5 mg/kg SeNPs + 2.0 mg/kg ZnNPs + 3.0 mg/kg SiNPs. The results showed significant differences (<em>P</em>≤0.05) between the treatments in the period from 22–38 days and 7–38 days. We observed that the birds in T2 consumed the most FI, while those in T3 and T4 consumed the least. The T4 group had a higher FCR than the other groups, but BWG and PI did not differ. The findings indicated no significant variations (<em>P</em>≤0.05) in carcass characteristics among the treatments. The outcomes found that administering SeNPs, ZnNPs, and SiNPs to birds significantly reduced their LDL, belly fat, and cholesterol. It also significantly impacted their T₃, T₄, IgA, and IgG levels and increased their HDL levels. In conclusion, SeNPs, ZnNPs, and SiNPs improved broiler performance, certain carcass features, gut microbial load, and improved meat quality for end consumers.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100552"},"PeriodicalIF":1.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise nutrition 2: Regression equations using KOH protein solubility to estimate digestible lysine and digestible arginine in commercial lots of soybean meal that have undergone overprocessing or auto combustion","authors":"Nelson Ruiz ,&nbsp;Carl M. Parsons ,&nbsp;Benjamin W. Parsons","doi":"10.1016/j.japr.2025.100551","DOIUrl":"10.1016/j.japr.2025.100551","url":null,"abstract":"<div><div>The objective of this study was to develop regression equations for lysine <strong>(Lys</strong>) and arginine (<strong>Arg</strong>) that would allow poultry nutritionists and formulators to estimate amino acid (<strong>AA</strong>) digestibility in commercial lots of solvent-extracted soybean meal (<strong>SBM</strong>) using a newly standardized protocol for KOH protein solubility (<strong>KOHPS</strong>). A total of 82 SBM samples, selected from 100 samples that were analyzed for in vivo AA digestibility, were analyzed for KOHPS. The KOHPS values ranged from 14 to 88 %, while digestible Lys coefficients ranged from 0.47 to 0.96. Digestible Arg coefficients ranged from 0.62 to 0.98. It became evident from total Lys and Arg analyses of the original samples that a net destruction of Lys and Arg occurred as KOHPS decreased; therefore, corrected digestibility coefficients were calculated using estimated total Lys and total Arg values. Estimated values were obtained utilizing regressions for each AA as a function of CP. Regressions on KOHPS were then run with and without corrected digestibility coefficients. Linear regressions provided the best fit for corrected digestible coefficients vs. KOHPS. The 1-slope broken line regression for Lys indicated that a plateau occurred at 80 % KOHPS and a digestible Lys coefficient of 0.88; therefore, an adequately processed solvent-extracted SBM should exhibit a minimum of 80 % KOHPS, which will yield an in vivo digestible Lys coefficient of 0.88. Equations developed from regression of digestible Lys and Arg coefficients against KOHPS herein will allow commercial poultry nutritionists and formulators to adjust AA digestibility in the formulation matrix for SBM according to its KOHPS.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100551"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the effect of the probiotic in the effectiveness of the Newcastle disease vaccine in specific pathogen-free chickens","authors":"Navid Asgharian ,&nbsp;Mohammad Amir Karimi Torshizi ,&nbsp;Naheed Mojgani ,&nbsp;Shaban Rahimi ,&nbsp;Mohammad Majid Ebrahimi","doi":"10.1016/j.japr.2025.100549","DOIUrl":"10.1016/j.japr.2025.100549","url":null,"abstract":"<div><div>The present study aimed to assess the impact of a probiotic supplement on the effectiveness of the Newcastle disease (<strong>ND</strong>) vaccine in specific pathogen-free (<strong>SPF</strong>) chickens. A total of one hundred and twenty 7-day-old SPF chicks were randomly divided into six groups including: CNT (control group), PRO (probiotic), RCV (Razi Clone12IR vaccine), ICV (imported Clone vaccine), PRO + RCV (probiotic and Razi Clone12IR vaccine), and PRO + ICV (probiotic and imported Clone vaccine). Administration of the probiotic, either alone or in conjunction with ND vaccination, decreased cholesterol and increased total protein, albumin, calcium, and phosphorus concentrations (<em>P &lt;</em> 0.05). Increasing weight, length, and bone breaking strength were observed in chickens that were given the probiotic alone. The highest concentration of malondialdehyde (<strong>MDA</strong>) was recorded in the CNT, while MDA levels decreased in the PRO, PRO + RCV, and PRO + ICV (<em>P &lt;</em> 0.05). An increase in IgY anti-SRBC antibody titer was found in the PRO + RCV and PRO + ICV groups. Higher antibody titer against ND was observed in the PRO + RCV (<em>P &lt;</em> 0.05). The use of probiotics during ND vaccination led to increased lysozyme activity compared to other groups (<em>P &lt;</em> 0.05). The birds receiving probiotics exhibited significantly greater villus height, crypt depth, and villus height/crypt depth ratio in the duodenum (<em>P &lt;</em> 0.05). No histopathological lesions were observed in the trachea, lungs, liver, or bursa of Fabricius in vaccinated chickens, regardless of whether they received probiotic dietary supplementation. The study’s findings suggest that incorporating probiotics during ND vaccination can enhance the health-related parameters of SPF chickens.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100549"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise nutrition 1: Standardization of the KOH protein solubility test for soybean meal in a single commercial laboratory and correlation to in vivo poultry amino acid digestibility","authors":"Nelson Ruiz ,&nbsp;Carl M. Parsons ,&nbsp;Brian Steinlicht ,&nbsp;Dave Taysom ,&nbsp;Xin Wu ,&nbsp;Fiona Liu","doi":"10.1016/j.japr.2025.100550","DOIUrl":"10.1016/j.japr.2025.100550","url":null,"abstract":"<div><div>The KOH protein solubility (<strong>KOHPS</strong>) method to evaluate if a lot of commercial soybean meal (<strong>SBM</strong>) has undergone the Maillard reaction during processing was introduced to the animal feed industry by Araba and Dale (1990). Although it is used worldwide, there are 2 limitations that have not been addressed. There is large inter-laboratory variability of the test and an absence of a quantitative interpretation of the results in terms of amino acid (<strong>AA</strong>) digestibility that can be used by nutritionists and formulators for commercial SBM affected by the Maillard reaction. The objective of this study was to standardize the KOHPS method in a single commercial laboratory starting from the description by Araba and Dale (1990) by using commercial SBM samples whose in vivo AA digestibility status was known. For this purpose, 2 SBM samples, one with high solubility (80 to 85 % KOHPS) and another with low solubility (KOHPS ∼ 50 %), were used as reference samples to develop a preliminary protocol. Then adjustments in intensity of agitation were made with 4 SBM samples of known in vivo digestible Lys coefficients (0.88 to 0.96) to yield KOHPS values from 80 % to 85 %. The standardized protocol yielded KOHPS values from 50 to 56 % for the low solubility SBM reference sample and 3 samples known to have low in vivo digestible Lys coefficients (0.73 to 0.77). In conclusion, a standardized protocol is now available for the determination of KOHPS of commercial SBM that will allow regression equations to be developed for the estimation of digestible AA.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100550"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}