Gustavo A.C.C. de Aguiar , Mariana A. do Nascimento , Nathana R. Furlani , Warley J. Alves , Gabriel S. Viana , Victor D. Naranjo , Melissa I. Hannas
{"title":"Using different statistical models to estimate the optimal methionine+cysteine to lysine ratio for male Cobb500 broilers: Growth performance, body composition, and meat yields","authors":"Gustavo A.C.C. de Aguiar , Mariana A. do Nascimento , Nathana R. Furlani , Warley J. Alves , Gabriel S. Viana , Victor D. Naranjo , Melissa I. Hannas","doi":"10.1016/j.japr.2025.100563","DOIUrl":"10.1016/j.japr.2025.100563","url":null,"abstract":"<div><div>Methionine (Met) is a vital sulfur amino acid essential for the survival, growth, and reproduction of poultry, - one which participates on multiple metabolic processes than exclusively protein synthesis. As feed ingredients naturally contains insufficient amounts of Met and cysteine (Cys), synthetic Met sources are commonly supplemented in feeds. The proper balance of Met and Cys is important not only for protein synthesis but also for improving feed efficiency and reducing environmental impacts, such as nitrogen emissions and water consumption. This study explored the effects of different digestible methionine + cysteine to lysine (Met+Cys:Lys) ratios on male Cobb500 broiler chicken performance across three growth phases: starter, grower, and finisher. The findings revealed that as birds matured, their Met+Cys:Lys ratios initially increased and then slightly decreased. Optimizing this ratio not only enhances growth and feed efficiency but also results in leaner carcasses with higher protein and mineral content. Additionally, these adjustments provide economic benefits by lowering feed costs and producing meat with less fat, resulting in a higher-quality product. This research emphasizes the importance of adjusting amino acid ratios based on growth phase to improve both broiler performance and meat yield.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100563"},"PeriodicalIF":1.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Garret G. Ashabranner, Michael Czarick III, Brian D. Fairchild
{"title":"Evaluating the effect of daylength (24, 20, and 18 hours) during brooding on broiler performance and physiological responses to light environment","authors":"Garret G. Ashabranner, Michael Czarick III, Brian D. Fairchild","doi":"10.1016/j.japr.2025.100558","DOIUrl":"10.1016/j.japr.2025.100558","url":null,"abstract":"<div><div>Brooding broiler chicks under continuous or near-continuous light is common in poultry research and commercial poultry production. Despite its prevalence, there is a notable lack of research to support its use. The current research investigated the impact of various daylengths (24, 20, and 18 hours) during brooding on broiler performance and physiological responses to the light environment. Broiler performance and physiological responses were assessed in four experiments comprised of ten trials. Control birds reared under continuous light during brooding initially exhibited higher body weight during the first week (<em>P</em> ≤ 0.05). Following the introduction of the dark period for the control groups on Day 7, the treatment birds subjected to dark periods during brooding overtook the control group in body weight. From Days 28-42, no differences in performance were observed between the control and treatment groups. No significant differences were found in feed conversion between treatment and control groups during any experiment. Corticosterone and superoxide dismutase were consistently unaffected by either lighting program. The introduction of a dark period during brooding led to higher baseline and nighttime elevation in melatonin levels in the treatment birds, persisting up to 35 days of age. Field trials conducted in commercial poultry houses further confirmed the research's findings, with no significant differences observed in performance or mortality when compared to control and treatment houses. This research illustrates that providing broiler chicks with a dark period from day of placement does not have detrimental effects on end-of-flock performance.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100558"},"PeriodicalIF":1.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catherine Fudge , Olivia Wedegaertner , Katherine Cupo , Justin Lowery , Lin Walker , Frank Edens , Chongxiao Chen
{"title":"Oral inoculation of turkeys with in vitro cultured Histomonas meleagridis in cecal contents results in histomonosis","authors":"Catherine Fudge , Olivia Wedegaertner , Katherine Cupo , Justin Lowery , Lin Walker , Frank Edens , Chongxiao Chen","doi":"10.1016/j.japr.2025.100560","DOIUrl":"10.1016/j.japr.2025.100560","url":null,"abstract":"<div><div>This study examined the effect of oral administration of <em>Histomonas meleagridis</em> on infection rate and disease progression of histomonosis in turkeys. Experiment 1, poults were placed in 3 experimental groups consisting of <em>H. meleagridis</em> culture in media administered via intracloacal inoculation (CIM), oral inoculation (OIM), or oral inoculation mixed with previously frozen cecal content (OICC). Poults were inoculated with 50,000 histomonads at 8 AM and 5 PM for five days starting from 14-day of age. Cloacal inoculation (CIM) produced 100 % infection rates, while oral infection with <em>H. meleagridis</em> in media (OIM) led to 8 % infection. However, oral inoculations using cecal content (OICC) produced 43 % infection rates (<em>P</em> = 0.0003). In experiment two, poults were placed in 2 experimental groups consisting oral inoculations with fresh cecal contents containing <em>H. meleagridis</em> (OI) and intracloacal inoculations with fresh cecal contents containing <em>H. meleagridis</em> (CI). Poults were inoculated with 50,000 histomonads at 8 AM and 5 PM for five days starting from 14-day of age. Oral inoculations (OI) produced 90 % infection rates, similar to the cloacal route (CI) at 93 % (<em>P</em> = 0.6433). In Experiment 3, individual isolates of <em>H. meleagridis</em> in cecal content were administered orally: Buford isolate (BUF), Arkansas isolate (ARK), or Zeeland, Michigan isolate (ZMI). Results showed BUF and ARK isolates produced higher infection rates than the ZMI isolate (<em>P</em> = 0.0136). In summary, oral infection in turkeys with <em>H. meleagridis</em> could be achieved using cecal content and infection rates and disease progression are impacted by isolate and inoculum condition.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100560"},"PeriodicalIF":1.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
He Huang , Size Wang , Chuicheng Zeng , Zhengyu Zang , Yue Pan , Yuanliang Zhang , Shan Yue , JunYing Wang , Peng Zheng , Xiuhua Zhao
{"title":"Study on the effects of anthocyanins on the growth performance, immunity, and meat quality of ZI geese","authors":"He Huang , Size Wang , Chuicheng Zeng , Zhengyu Zang , Yue Pan , Yuanliang Zhang , Shan Yue , JunYing Wang , Peng Zheng , Xiuhua Zhao","doi":"10.1016/j.japr.2025.100562","DOIUrl":"10.1016/j.japr.2025.100562","url":null,"abstract":"<div><div>Zi goose is an excellent local breed with fast growth, strong disease resistance, and good meat quality in China. Anthocyanins are natural water-soluble pigments with various biological functions such as promoting growth, antioxidation, and immune regulation. However, research on the beneficial effects of anthocyanins on geese is not yet in-depth. The aim of this study is to investigate the effects of anthocyanins on the growth performance, immunity, and meat quality of Zi geese, providing reference for healthy culture of geese. Two hundred 28-day-old healthy geese were randomly divided into 4 groups with 5 replicates in each group and 10 geese per replicate. Anthocyanins (0, 50, 100, and 200 mg/kg) were added to the goose dietary of the control group (CG), the anthocyanin group 1 (AG1), anthocyanin group 2 (AG2), and anthocyanin group 3 (AG3), respectively. The experimental period was 10 weeks. The results showed that anthocyanin decreased the levels of the interleukin-6 and tumor necrosis factor-α, the abdominal fat percentage, the shear force and drip loss of breast muscle, and increased the levels of immunoglobulin M and interleukin-10, the number of blood lymphocytes, the pH<sub>24h</sub> value and a* value of breast muscle. In conclusion, anthocyanins can improve immunity, and meat quality, while reduce abdominal fat percentage in Zi geese. The optimum additive amount of anthocyanin was 100 mg/kg in Zi goose.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100562"},"PeriodicalIF":1.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T.W. Liu , J.Y. Chen , J.G. Zhu , L.X. Wang , X.M. Meng , H.X. Huan , Y. Liu , P. Xie
{"title":"Effects of different feeding strategies on the performance and meat quality of pigeon squabs (Columba livia) in the late stage of growth","authors":"T.W. Liu , J.Y. Chen , J.G. Zhu , L.X. Wang , X.M. Meng , H.X. Huan , Y. Liu , P. Xie","doi":"10.1016/j.japr.2025.100561","DOIUrl":"10.1016/j.japr.2025.100561","url":null,"abstract":"<div><div>The current study investigated the effects of two different feeding strategies on the performance and meat quality of squabs (White King pigeon). A total of 108, 21-day-old squabs were randomly divided into three artificial crop milk treatments with different crude protein (<strong>CP</strong>) levels (14%, 15%, 16%). The parent-fed squab control treatment consisted of 36 pairs of pigeons caring for 2 squabs. Results showed that the 28-day body weight, average body weight gain, index of abdominal fat and breast muscle, muscle shearing force, and fiber cross-sectional area and diameter of squabs in group 1 (16% CP) were the highest. In addition, the low dripping and freezing losses and the minimum malondialdehyde content of muscles in group 1 (16 % CP) were observed. Meanwhile, the activity of glutathione peroxidase in the muscle of the control group was the lowest. The values of redness and yellowness of muscles were higher in the control group than those in the artificial feeding treatments. In conclusion, although the muscle color of artificially fed pigeons is not as good as that of naturally fed ones, the 16% CP artificial diet significantly promoted the growth performance and muscle antioxidant capacity of squabs. Therefore, using 16% CP level of diet for artificially feeding squabs during the late stage of growth is recommended.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100561"},"PeriodicalIF":1.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mercy C. Ogwuegbu , Prosper Chukwudi , Chukwuebuka Okafor-Paul , Henry O. Edeh , Augustine O. Ani , Uchele Okpanachi , Doctor M.N. Mthiyane
{"title":"Effect of varying levels of benzoic acid on growth performance, haemato-biochemical indices, carcass traits, gut morphology and intestinal microbiota of broiler chickens","authors":"Mercy C. Ogwuegbu , Prosper Chukwudi , Chukwuebuka Okafor-Paul , Henry O. Edeh , Augustine O. Ani , Uchele Okpanachi , Doctor M.N. Mthiyane","doi":"10.1016/j.japr.2025.100556","DOIUrl":"10.1016/j.japr.2025.100556","url":null,"abstract":"<div><div>This study evaluated the effects of varying dietary levels of benzoic acid on the growth performance, haemato-biochemical indices, carcass traits, gut morphology, and intestinal microbiota of broiler chickens. A total of 300 day-old male Arbor acre broiler chicks were assigned to five dietary treatments supplemented with 0, 2.5, 5.0. 7.5, and 10.0 g/kg benzoic acid in a completely randomized design for 42 days. Results indicated that dietary inclusion of benzoic acid significantly influenced the growth performance, with the highest body weight gain (BWG) and improved feed conversion ratio (FCR) observed at 5.0 g/kg inclusion level (<em>P</em> < 0.001). Haemtao-biochemical indices showed that total protein and globulin levels were significantly higher (<em>P</em> < 0.05) at 7.5 and 10.0g/kg, whereas cholesterol, glucose, and uric acid levels decreased with increasing benzoic acid inclusion (<em>P</em> < 0.05). Carcass traits significantly improved at 5.0 g/kg, with higher breast and thigh muscle yields (<em>P</em> < 0.05). Gut morphology was significantly enhanced, with increased villus length, crypt depth, and epithelium thickness at 5.0 and 7.5 g/kg (<em>P</em> < 0.05). The intestinal microbial analysis revealed a dose-dependent effect, <em>Lactobacillus</em> and B<em>ifidobacteria</em> peaked at 7.5 g/kg, while S<em>almonella</em> decreased progressively with increasing benzoic acid levels (<em>P</em> < 0.001). In conclusion, benzoic acid supplementation at 5.0 g/kg optimally enhances broiler performance, gut health, and microbial balance, whereas higher inclusion levels may induce metabolic adaptations or stress responses.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100556"},"PeriodicalIF":1.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A horizontal mixer and a batch-to-horizontal mixer system increased the mix uniformity of free methionine in complete diets relative to a vertical mixer","authors":"L.E. Knarr , K.M. Bowen , E.B. Estanich , J.S. Moritz","doi":"10.1016/j.japr.2025.100554","DOIUrl":"10.1016/j.japr.2025.100554","url":null,"abstract":"<div><div>The coefficient of variation (CV) of a selected nutrient marker is commonly utilized to measure mix uniformity (MU) of a batch of feed, where a CV < 10 % is considered industry-acceptable. Mixer type and mix time (MTI) have been shown to affect mix uniformity (MU), however, data concerning mixing systems (MSY) are unavailable. Additionally, past research has shown that the selection of an appropriate CV marker is integral in properly evaluating MU. Two experiments were conducted to evaluate a vertical, horizontal, and transfer MSY on MU while utilizing inadequate or adequate MTIs for a corn-salt mixture (CSM) and complete diet (CD). Chloride ion (Cl<sup>−</sup>) was selected as the marker for the CSM, and crystalline DL-Methionine (Free Met) and crystalline l-Lysine-HCl (Free Lys) were selected as the markers for the CD. Increasing MTI decreased Cl<sup>−</sup> CV in the CSM (<em>P</em> = 0.020). An interaction between MSY and MTI affected Free Met CV in the CD (<em>P</em> = 0.007). The horizontal and transfer MSYs did not demonstrate changes in the Free Met CV when MTI was manipulated, whereas the vertical MSY provided decreased CV with increased MTI. The authors hypothesize that additional mixing during load-out and conveyance caused this interaction. However, no treatment provided a Free Lys CV < 10 %. These data suggest that Cl<sup>−</sup> and Free Met are appropriate markers for MU in CSMs and CDs respectively, transfer MSYs may improve the overall efficiency of feed manufacture, and evaluating MSYs as a whole may provide more representative evaluations of feed before animal consumption.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100554"},"PeriodicalIF":1.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elwy A. Ashour , Ahmed K. Aldhalmi , Mahmoud Kamal , Azza R.F. El dosokey , Ayman A. Swelum
{"title":"Growth, carcass characteristics, meat quality, blood indices and gut microbiota of broiler chickens fed diets enriched with a combination of organic Zn, Se and Si nanoparticles","authors":"Elwy A. Ashour , Ahmed K. Aldhalmi , Mahmoud Kamal , Azza R.F. El dosokey , Ayman A. Swelum","doi":"10.1016/j.japr.2025.100552","DOIUrl":"10.1016/j.japr.2025.100552","url":null,"abstract":"<div><div>This research studied the impacts of feeding different amounts of ZnNPs, SeNPs, and SiNPs on the carcass, gut microbiota, meat quality, growth performance, and blood markers in broilers up to 38 days old. For a total of 300 chicks, five groups were given six replications of ten unsexed one-week broiler chicks (Cobb). At various doses, the five experimental treatment groups received corn-soybean meal-based nutritional supplements: 0.0, 1.5 mg/kg SeNPs + 2.0 mg/kg ZnNPs, 1.5 mg/kg SeNPs + 3.0 mg/kg SiNPs, 2.0 mg/kg ZnNPs + 3.0 mg/kg SiNPs, and 1.5 mg/kg SeNPs + 2.0 mg/kg ZnNPs + 3.0 mg/kg SiNPs. The results showed significant differences (<em>P</em>≤0.05) between the treatments in the period from 22–38 days and 7–38 days. We observed that the birds in T2 consumed the most FI, while those in T3 and T4 consumed the least. The T4 group had a higher FCR than the other groups, but BWG and PI did not differ. The findings indicated no significant variations (<em>P</em>≤0.05) in carcass characteristics among the treatments. The outcomes found that administering SeNPs, ZnNPs, and SiNPs to birds significantly reduced their LDL, belly fat, and cholesterol. It also significantly impacted their T<sub>3</sub>, T<sub>4</sub>, IgA, and IgG levels and increased their HDL levels. In conclusion, SeNPs, ZnNPs, and SiNPs improved broiler performance, certain carcass features, gut microbial load, and improved meat quality for end consumers.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100552"},"PeriodicalIF":1.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nelson Ruiz , Carl M. Parsons , Benjamin W. Parsons
{"title":"Precise nutrition 2: Regression equations using KOH protein solubility to estimate digestible lysine and digestible arginine in commercial lots of soybean meal that have undergone overprocessing or auto combustion","authors":"Nelson Ruiz , Carl M. Parsons , Benjamin W. Parsons","doi":"10.1016/j.japr.2025.100551","DOIUrl":"10.1016/j.japr.2025.100551","url":null,"abstract":"<div><div>The objective of this study was to develop regression equations for lysine <strong>(Lys</strong>) and arginine (<strong>Arg</strong>) that would allow poultry nutritionists and formulators to estimate amino acid (<strong>AA</strong>) digestibility in commercial lots of solvent-extracted soybean meal (<strong>SBM</strong>) using a newly standardized protocol for KOH protein solubility (<strong>KOHPS</strong>). A total of 82 SBM samples, selected from 100 samples that were analyzed for in vivo AA digestibility, were analyzed for KOHPS. The KOHPS values ranged from 14 to 88 %, while digestible Lys coefficients ranged from 0.47 to 0.96. Digestible Arg coefficients ranged from 0.62 to 0.98. It became evident from total Lys and Arg analyses of the original samples that a net destruction of Lys and Arg occurred as KOHPS decreased; therefore, corrected digestibility coefficients were calculated using estimated total Lys and total Arg values. Estimated values were obtained utilizing regressions for each AA as a function of CP. Regressions on KOHPS were then run with and without corrected digestibility coefficients. Linear regressions provided the best fit for corrected digestible coefficients vs. KOHPS. The 1-slope broken line regression for Lys indicated that a plateau occurred at 80 % KOHPS and a digestible Lys coefficient of 0.88; therefore, an adequately processed solvent-extracted SBM should exhibit a minimum of 80 % KOHPS, which will yield an in vivo digestible Lys coefficient of 0.88. Equations developed from regression of digestible Lys and Arg coefficients against KOHPS herein will allow commercial poultry nutritionists and formulators to adjust AA digestibility in the formulation matrix for SBM according to its KOHPS.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100551"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Navid Asgharian , Mohammad Amir Karimi Torshizi , Naheed Mojgani , Shaban Rahimi , Mohammad Majid Ebrahimi
{"title":"Assessing the effect of the probiotic in the effectiveness of the Newcastle disease vaccine in specific pathogen-free chickens","authors":"Navid Asgharian , Mohammad Amir Karimi Torshizi , Naheed Mojgani , Shaban Rahimi , Mohammad Majid Ebrahimi","doi":"10.1016/j.japr.2025.100549","DOIUrl":"10.1016/j.japr.2025.100549","url":null,"abstract":"<div><div>The present study aimed to assess the impact of a probiotic supplement on the effectiveness of the Newcastle disease (<strong>ND</strong>) vaccine in specific pathogen-free (<strong>SPF</strong>) chickens. A total of one hundred and twenty 7-day-old SPF chicks were randomly divided into six groups including: CNT (control group), PRO (probiotic), RCV (Razi Clone12IR vaccine), ICV (imported Clone vaccine), PRO + RCV (probiotic and Razi Clone12IR vaccine), and PRO + ICV (probiotic and imported Clone vaccine). Administration of the probiotic, either alone or in conjunction with ND vaccination, decreased cholesterol and increased total protein, albumin, calcium, and phosphorus concentrations (<em>P <</em> 0.05). Increasing weight, length, and bone breaking strength were observed in chickens that were given the probiotic alone. The highest concentration of malondialdehyde (<strong>MDA</strong>) was recorded in the CNT, while MDA levels decreased in the PRO, PRO + RCV, and PRO + ICV (<em>P <</em> 0.05). An increase in IgY anti-SRBC antibody titer was found in the PRO + RCV and PRO + ICV groups. Higher antibody titer against ND was observed in the PRO + RCV (<em>P <</em> 0.05). The use of probiotics during ND vaccination led to increased lysozyme activity compared to other groups (<em>P <</em> 0.05). The birds receiving probiotics exhibited significantly greater villus height, crypt depth, and villus height/crypt depth ratio in the duodenum (<em>P <</em> 0.05). No histopathological lesions were observed in the trachea, lungs, liver, or bursa of Fabricius in vaccinated chickens, regardless of whether they received probiotic dietary supplementation. The study’s findings suggest that incorporating probiotics during ND vaccination can enhance the health-related parameters of SPF chickens.</div></div>","PeriodicalId":15240,"journal":{"name":"Journal of Applied Poultry Research","volume":"34 3","pages":"Article 100549"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}