Assessing the effect of the probiotic in the effectiveness of the Newcastle disease vaccine in specific pathogen-free chickens

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Applied Poultry Research Pub Date : 2025-05-15 DOI:10.1016/j.japr.2025.100549

Navid Asgharian , Mohammad Amir Karimi Torshizi , Naheed Mojgani , Shaban Rahimi , Mohammad Majid Ebrahimi

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引用次数: 0

Abstract

The present study aimed to assess the impact of a probiotic supplement on the effectiveness of the Newcastle disease (ND) vaccine in specific pathogen-free (SPF) chickens. A total of one hundred and twenty 7-day-old SPF chicks were randomly divided into six groups including: CNT (control group), PRO (probiotic), RCV (Razi Clone12IR vaccine), ICV (imported Clone vaccine), PRO + RCV (probiotic and Razi Clone12IR vaccine), and PRO + ICV (probiotic and imported Clone vaccine). Administration of the probiotic, either alone or in conjunction with ND vaccination, decreased cholesterol and increased total protein, albumin, calcium, and phosphorus concentrations (P < 0.05). Increasing weight, length, and bone breaking strength were observed in chickens that were given the probiotic alone. The highest concentration of malondialdehyde (MDA) was recorded in the CNT, while MDA levels decreased in the PRO, PRO + RCV, and PRO + ICV (P < 0.05). An increase in IgY anti-SRBC antibody titer was found in the PRO + RCV and PRO + ICV groups. Higher antibody titer against ND was observed in the PRO + RCV (P < 0.05). The use of probiotics during ND vaccination led to increased lysozyme activity compared to other groups (P < 0.05). The birds receiving probiotics exhibited significantly greater villus height, crypt depth, and villus height/crypt depth ratio in the duodenum (P < 0.05). No histopathological lesions were observed in the trachea, lungs, liver, or bursa of Fabricius in vaccinated chickens, regardless of whether they received probiotic dietary supplementation. The study’s findings suggest that incorporating probiotics during ND vaccination can enhance the health-related parameters of SPF chickens.

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评价益生菌对新城疫疫苗对特定无病原体鸡的有效性的影响

本研究旨在评估益生菌补充剂对新城疫（ND）疫苗在特定无病原体（SPF）鸡中有效性的影响。选取120只7日龄SPF雏鸡，随机分为6组：CNT（对照组）、PRO（益生菌）、RCV （Razi Clone12IR疫苗）、ICV（进口克隆疫苗）、PRO + RCV（益生菌和Razi Clone12IR疫苗）、PRO + ICV（益生菌和进口克隆疫苗）。单独或与新城疫疫苗联合使用益生菌可降低胆固醇，增加总蛋白、白蛋白、钙和磷浓度(P <；0.05)。在单独给予益生菌的鸡中，观察到体重，长度和骨折强度增加。丙二醛（MDA）浓度在CNT中最高，而MDA水平在PRO、PRO + RCV和PRO + ICV中下降(P <；0.05)。在PRO + RCV和PRO + ICV组中发现IgY抗srbc抗体滴度升高。PRO + RCV中抗ND抗体滴度较高(P <；0.05)。与其他组相比，在ND疫苗接种期间使用益生菌导致溶菌酶活性增加(P <；0.05)。益生菌组的十二指肠绒毛高度、隐窝深度和绒毛高度/隐窝深度比(P <；0.05)。无论是否补充益生菌，接种疫苗的鸡的气管、肺、肝脏或法氏囊均未观察到组织病理学病变。研究结果表明，在ND疫苗接种过程中加入益生菌可以提高SPF鸡的健康相关参数。

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来源期刊

Journal of Applied Poultry Research 农林科学-奶制品与动物科学

CiteScore

4.10

自引率

10.50%

发文量

审稿时长

104 days

期刊介绍： The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.