Precise nutrition 1: Standardization of the KOH protein solubility test for soybean meal in a single commercial laboratory and correlation to in vivo poultry amino acid digestibility

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Applied Poultry Research Pub Date : 2025-05-15 DOI:10.1016/j.japr.2025.100550

Nelson Ruiz , Carl M. Parsons , Brian Steinlicht , Dave Taysom , Xin Wu , Fiona Liu

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Abstract

The KOH protein solubility (KOHPS) method to evaluate if a lot of commercial soybean meal (SBM) has undergone the Maillard reaction during processing was introduced to the animal feed industry by Araba and Dale (1990). Although it is used worldwide, there are 2 limitations that have not been addressed. There is large inter-laboratory variability of the test and an absence of a quantitative interpretation of the results in terms of amino acid (AA) digestibility that can be used by nutritionists and formulators for commercial SBM affected by the Maillard reaction. The objective of this study was to standardize the KOHPS method in a single commercial laboratory starting from the description by Araba and Dale (1990) by using commercial SBM samples whose in vivo AA digestibility status was known. For this purpose, 2 SBM samples, one with high solubility (80 to 85 % KOHPS) and another with low solubility (KOHPS ∼ 50 %), were used as reference samples to develop a preliminary protocol. Then adjustments in intensity of agitation were made with 4 SBM samples of known in vivo digestible Lys coefficients (0.88 to 0.96) to yield KOHPS values from 80 % to 85 %. The standardized protocol yielded KOHPS values from 50 to 56 % for the low solubility SBM reference sample and 3 samples known to have low in vivo digestible Lys coefficients (0.73 to 0.77). In conclusion, a standardized protocol is now available for the determination of KOHPS of commercial SBM that will allow regression equations to be developed for the estimation of digestible AA.

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精准营养1：单一商业实验室豆粕KOH蛋白溶解度试验标准化及与家禽体内氨基酸消化率的相关性

阿拉巴和戴尔（1990）将KOH蛋白溶解度（KOHPS）方法引入动物饲料行业，以评估大量商品豆粕（SBM）在加工过程中是否发生了美拉德反应。虽然它在世界范围内使用，但有两个限制尚未解决。实验室间的测试存在很大的差异，并且缺乏对氨基酸消化率结果的定量解释，营养学家和配方师可以使用氨基酸消化率来为受美拉德反应影响的商业SBM制定配方。本研究的目的是从Araba和Dale（1990）的描述开始，通过使用已知体内AA消化率的商业SBM样品，在单一商业实验室中标准化KOHPS方法。为此，使用2个SBM样品，一个具有高溶解度（80 ~ 85% KOHPS），另一个具有低溶解度（KOHPS ~ 50%），作为参考样品来制定初步方案。然后用4个已知体内可消化赖氨酸系数（0.88 ~ 0.96）的SBM样品调整搅拌强度，使KOHPS值在80% ~ 85%之间。对于低溶解度的SBM参考样品和已知具有低体内可消化赖氨酸系数（0.73至0.77）的3个样品，标准化方案的KOHPS值为50至56%。综上所述，现在有一个标准化的方案可用于测定商业SBM的KOHPS，这将允许开发回归方程来估计可消化的AA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Poultry Research 农林科学-奶制品与动物科学

CiteScore

4.10

自引率

10.50%

发文量

审稿时长

104 days

期刊介绍： The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.