Journal of Cellular Physiology最新文献

{"title":"Expression OF Concern: Increased Autocrine Interleukin-6 Production Is Significantly Associated With Worse Clinical Outcome in Patients With Chronic Lymphocytic Leukemia","authors":"","doi":"10.1002/jcp.70221","DOIUrl":"https://doi.org/10.1002/jcp.70221","url":null,"abstract":"<p>EXPRESSION OF CONCERN: H.-Q. Wang, L. Jia, Y.-T. Li, T. Farren, S. G. Agrawal, and F.-T. Liu, “Increased Autocrine Interleukin-6 Production Is Significantly Associated With Worse Clinical Outcome in Patients With Chronic Lymphocytic Leukemia,” <i>Journal of Cellular Physiology</i> 234, no. 8 (2019): 13994-14006, https://doi.org/10.1002/jcp.28086</p><p>This Expression of Concern is for the above article, published online on 08 January 2019 in Wiley Online Library (https://onlinelibrary.wiley.com/), and has been issued by agreement between the journal Editor-in-Chief, Robert Heath; and Wiley Periodicals LLC. The Expression of Concern has been agreed due to concerns raised by third parties. Specifically, a duplication has been identified between the β-actin blot presented in Figure 4c and the COX IV blot presented in Figure 5b. The authors could not be reached to provide comments or clarification regarding these concerns. Therefore, the journal has decided to issue an Expression of Concern to inform and alert the readers.</p>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcp.70221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenylyl Cyclase (ADCY) Protein Family: Molecular Mechanisms and Therapeutic Potential in Glucose and Lipid Metabolism and Feeding Regulation","authors":"Shaohong Wu,&nbsp;Yongxue Chen,&nbsp;Jiali Xueting Zhang,&nbsp;Jinshan Zhang","doi":"10.1002/jcp.70216","DOIUrl":"https://doi.org/10.1002/jcp.70216","url":null,"abstract":"<div>\u0000 \u0000 <p>The adenylyl cyclase (ADCY) family comprises key signal transduction enzymes that catalyze the conversion of ATP to the second messenger cyclic adenosine monophosphate (cAMP). Through the downstream cAMP–protein kinase A (PKA) signaling pathway, this family exerts central regulatory effects on glucose and lipid homeostasis. In mammals, the ADCY family consists of nine transmembrane isoforms (ADCY1–9) and one soluble isoform (ADCY10), all of which exhibit tissue‑specific distribution, distinct regulatory mechanisms, and non‑redundant functions in core metabolic organs. Compelling evidence from gene‑edited animal models, functional validation studies, and multi‑ethnic genetic association analyses has demonstrated that dysfunction of specific ADCY isoforms is closely linked to the pathogenesis of obesity, type 2 diabetes mellitus (T2DM), metabolic dysfunction‑associated steatotic liver disease (MASLD), and other metabolic disorders. This review systematically summarizes the classification, structural characteristics, and multilayered regulatory mechanisms of the ADCY family, with a particular focus on the physiological and pathological roles of its isoforms in glucose metabolism, lipid metabolism, and feeding regulation. It also discusses recent advances in ADCY‑targeted agents and highlights unresolved challenges and future directions. Particular emphasis is given to recent structural biology breakthroughs, genetic evidence from diverse populations, and isoform‑specific therapeutic strategies, with the aim of providing a theoretical basis for mechanistic research and the development of targeted therapeutics for metabolic diseases.</p>\u0000 </div>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulated Microgravity Induced Mesenchymal Stem Cell Senescence via the Activation of Cytosolic mtDNA-cGAS-STING Axis","authors":"Lei Huang,&nbsp;Rui Huang,&nbsp;Wenjun Lv,&nbsp;Zimeng Li,&nbsp;Yun Tu,&nbsp;Guanbin Song,&nbsp;Qing Luo","doi":"10.1002/jcp.70220","DOIUrl":"10.1002/jcp.70220","url":null,"abstract":"<div>\u0000 \u0000 <p>Microgravity brings various pathological changes to astronauts, which may be related to the senescence of adult stem cells. However, how microgravity affects the senescence of stem cells remains barely known. In this study, it was found that microgravity simulated by Rotating Flat Chamber induced MSC senescence and promoted the expression of cyclic GMP-AMP synthase (cGAS) and stimulator of interferon genes (STING), and C176, a STING inhibitor, alleviated the rotating culture-induced MSC senescence. Rotating culture also promoted the cytosolic leakage of mitochondrial DNA (mtDNA), while the depletion of mtDNA inhibited cGAS-STING activation and reversed MSC senescence. Knockdown of either BAK or BAX, proteins forming pores on the mitochondrial outer membrane, suppressed mtDNA leakage, cGAS-STING activation, and MSC senescence, suggesting that BAK/BAX mediates the activation of mtDNA-cGAS-STING axis and the associated MSC senescence. Recovering mitochondrial function by Mito TEMPO inhibited the activation of mtDNA-cGAS-STING axis and reversed MSC senescence. In vivo, using rat hindlimb unloading (HU) model to simulate microgravity, it was found that inhibition of STING ameliorated MSC senescence induced by HU. Together, our study demonstrated that simulated microgravity induces MSC senescence by the activation of cytosolic mtDNA-cGAS-STING axis and indicated cGAS-STING pathway as a therapeutic target of MSC senescence under microgravity.</p></div>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Welcome to the Relaunched Journal of Cellular Physiology","authors":"Paul Trevorrow,&nbsp;Robert J. W. Heath","doi":"10.1002/jcp.70218","DOIUrl":"10.1002/jcp.70218","url":null,"abstract":"","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hippo Pathway Regulation and Microenvironmental Cues Governing Human MSC Transition to Cancer-Associated Fibroblasts","authors":"Pimjai Chingsuwanrote,&nbsp;Chanchao Lorthongpanich,&nbsp;Pakpoom Kheolamai,&nbsp;Ting Gang Chew,&nbsp;Chuti Laowtammathron,&nbsp;Sudjit Luanpitpong,&nbsp;Rangsun Parnpai,&nbsp;Surapol Issaragrisil","doi":"10.1002/jcp.70219","DOIUrl":"10.1002/jcp.70219","url":null,"abstract":"<p>Cancer-associated fibroblasts (CAFs) are key drivers of tumor progression. This study examined how three-dimensional (3D) culture, hypoxia, and cancer-derived soluble factors influence the transformation of human mesenchymal stem cells (hMSCs) into inflammatory CAFs (iCAFs). hMSCs from bone marrow, placenta, and chorion were cultured in 2D, in Matrigel-based 3D systems, under hypoxia, and with soluble factors from colon cancer cells (HT29, HCT116). 3D culture strongly induced iCAF markers (IL1α, CSF3, IL6) while reducing myofibroblastic CAF markers (CCN2, MYL9, TAGLN). Hypoxia and cancer factors further enhanced this phenotype, promoting IL1α/IL6 secretion and shifting their influence from suppressing to stimulating cancer cell growth and angiogenesis. Mechanistically, these changes were associated with YAP/TAZ down-regulation, and genetic depletion of YAP/TAZ alone was sufficient to convert hMSCs into iCAFs even in 2D culture. These findings highlight YAP/TAZ as critical regulators of hMSC-to-CAF transformation, with implications for therapeutic strategies targeting tumor stroma.</p>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13440140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics Profiling Identifies Blood-Based Diagnostic Markers for Sepsis","authors":"Xin Zhang,&nbsp;Xiaoqing Guo,&nbsp;Shuai Jiang,&nbsp;Yanting Cao,&nbsp;Shuya Wang,&nbsp;Wentao Xie,&nbsp;Jiayin Tian,&nbsp;Yazhe Zhou,&nbsp;Man Wang,&nbsp;Hui Chang,&nbsp;Yang Yang","doi":"10.1002/jcp.70213","DOIUrl":"https://doi.org/10.1002/jcp.70213","url":null,"abstract":"<div>\u0000 \u0000 <p>Sepsis, characterized by a rapid transition to systemic immune dysregulation and multiorgan failure, poses a formidable clinical challenge. The lack of spatiotemporally stable biomarkers severely impedes early diagnosis and risk stratification. By integrating large-scale transcriptomic profiling with machine learning algorithms, this study identified a robust three-gene diagnostic signature (TLR5, HMGB2, and C19orf59). Single-cell RNA sequencing precisely localized the sepsis-induced specific upregulation of these targets to the myeloid immune compartment, notably monocytes and neutrophils. Crucially, disease severity stratification analysis (based on SOFA scores) revealed that while TLR5 and HMGB2 excel in identifying high-risk sepsis, C19orf59 maintains highly consistent diagnostic efficacy across all clinical severity strata. To definitively validate these findings while rigorously eliminating confounding effects from surgical or environmental stress, strictly time-matched sham-controlled cecal ligation and puncture (CLP) murine models and vehicle-controlled <i>in vitro</i> models were utilized. <i>In vivo</i> results corroborated the persistent in situ upregulation of this signature across vital target organs (lung, heart, liver) and systemic circulation. Parallel <i>in vitro</i> lipopolysaccharide (LPS)-stimulated cellular models further characterized their dynamic expression, with HMGB2 exhibiting a distinct biphasic kinetic profile mechanistically characteristic of danger-associated molecular patterns (DAMPs). Finally, independent clinical validation using sepsis patient serum corroborated the translational relevance of these targets. Collectively, this systematic multidimensional evaluation establishes TLR5, HMGB2, and C19orf59 as a highly reliable diagnostic and severity-stratification panel, providing novel molecular insights into the septic pathological cascade.</p></div>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Neural Crest Cells in Establishing Corneal Transparency During Embryonic Development in Mice","authors":"Djida Ghoubay,&nbsp;Cécile Vidal,&nbsp;Quentin Rappeneau,&nbsp;Jasmina Emini,&nbsp;Yorick Gitton,&nbsp;Stéphane Fouquet,&nbsp;Marie-Claire Schanne Klein,&nbsp;Karsten Plamann,&nbsp;Gaël Latour,&nbsp;Vincent Borderie","doi":"10.1002/jcp.70215","DOIUrl":"https://doi.org/10.1002/jcp.70215","url":null,"abstract":"<div>\u0000 \u0000 <p>Corneal transparency emerges during embryogenesis through the coordinated organization of neural crest–derived cells (NCCs) and extracellular matrix (ECM), yet the temporal and structural basis of this process remains incompletely defined. Here, we provide a multimodal, spatiotemporal analysis of mouse corneal development from embryonic Day 10 (E10) to birth (P0), combining whole-mount and sectioned immunofluorescence, tissue clearing with three-dimensional (3D) imaging, second harmonic generation (SHG) microscopy, full-field optical coherence microscopy (FFOCM), and transmission electron microscopy (TEM). We show that early periocular mesenchyme is characterized by broad expression of neural crest-associated markers (Sox9, HNK1), followed by progressive spatial restriction and downregulation as cells populate the corneal stroma. Sox10-positive cells remain primarily associated with developing nerves and are largely excluded from the stromal compartment. Concomitantly, stromal cells undergo marked morphological transitions, from rounded to progressively flattened and elongated phenotypes, accompanying stromal expansion. Collagen deposition is first detected in the subepithelial region around E12 and increases thereafter, with SHG and TEM analyses revealing progressive organization and compaction of fibrillar networks. Quantitative ultrastructural analysis indicates dynamic changes in interfibrillar spacing during development, consistent with ongoing ECM remodeling. The emergence of a posterior-to-anterior gradient in cell morphology and matrix organization suggests a spatially coordinated maturation process across the stroma. Together, these findings provide an integrated structural framework linking NCCs dynamics, stromal cell differentiation, and ECM organization during murine corneal development. This work establishes a quantitative and multiscale atlas of corneal morphogenesis that informs how tissue architecture compatible with transparency is progressively established in vivo.</p></div>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Ageing on Skeletal Muscle: Roles of Mitochondrial Dysregulation, Systemic Communication, and Exercise","authors":"Juan Diego Hernández-Camacho,&nbsp;Marielle Saclier","doi":"10.1002/jcp.70212","DOIUrl":"10.1002/jcp.70212","url":null,"abstract":"<p>Ageing is a major risk factor for degenerative diseases, including sarcopenia, which is characterized by a progressive loss of skeletal muscle mass and function, frailty, and is associated with increased mortality. Skeletal muscle regeneration relies on muscle stem cells and efficient communication with cellular microenvironment. With ageing, skeletal muscle regenerative capacity declines, and sarcopenia results from complex, multitissue dysregulation involving mitochondrial dysfunction, immune ageing, chronic inflammation, senescence, extracellular matrix modification, disruption of neuromuscular junctions and muscle-specific vulnerability. This review summarizes current knowledge contributing to sarcopenia and inefficient muscle repair during ageing from cell-autonomous metabolic dysregulation to age-associated changes in the local and systemic cellular environment. We also explore recent insights into important role of exercise on muscle tissue health. Overall, emerging technologies, including human muscle atlases and spatial transcriptomics, together with exercise-based interventions, will help to identify of novel biomarkers and therapeutic targets to better prevent and treat sarcopenia.</p>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 8","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13402775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148591438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arginine Methylation in Alternative Splicing: Implications for Cancer Therapy","authors":"Youyue Li,&nbsp;Shuyu Chen,&nbsp;Wenbo Ding,&nbsp;Bei Pan,&nbsp;Yujuan Yin,&nbsp;Xiaoqi Xin,&nbsp;Die Xia,&nbsp;Xueyang Wei,&nbsp;Siqi Bao,&nbsp;Mu Xu","doi":"10.1002/jcp.70206","DOIUrl":"10.1002/jcp.70206","url":null,"abstract":"<div>\u0000 \u0000 <p>Arginine methylation is a common post-translational modification that exists in three distinct forms—monomethylation, asymmetric dimethylation, and symmetric dimethylation—through which it regulates precursor RNA splicing and maintains cellular homeostasis. Dysregulation of the writing, reading, or erasure of arginine methylation promotes cancer development. Recent studies have identified PRMTs as key regulators of alternative splicing, and aberrant PRMT-driven splicing directly impacts multiple biological processes, including tumor proliferation, apoptosis resistance, metastasis, and immune evasion. This review focuses on the molecular mechanisms by which PRMTs regulate alternative splicing, their connections to oncogenic processes, and the therapeutic implications and challenges of targeting the PRMT-splicing axis in cancer.</p></div>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESSION OF CONCERN: Castration-Induced Prostate Epithelial Cell Apoptosis Results From Targeted Oxidative Stress Attack of M1142-Macrophages","authors":"","doi":"10.1002/jcp.70195","DOIUrl":"10.1002/jcp.70195","url":null,"abstract":"<p><b>EXPRESSION OF CONCERN</b>: G. O. Barbosa, J. A. F. Silva, A. Siqueira-Berti, U. Nishan, R. Rosa-Ribeiro, S. B. P. Oliveira, M. O. Baratti, D. Ferrucci, J. C. O. Santana, D. M. Damas-Souza, A. Bruni-Cardoso, T. M. Augusto, F. Corrêa-da-Silva, P. M. Moraes-Vieira, D. R. Stach-Machado, S. L. Felisbino, G. B. Menezes, C. L. Cesar, and H. F. Carvalho, “Castration-induced prostate epithelial cell apoptosis results from targeted oxidative stress attack of M1₁₄₂-macrophages,” <i>Journal of Cellular Physiology</i> 234, no. 10 (2019): 19048–19058, https://doi.org/10.1002/jcp.28544.</p><p>This Expression of Concern is for the above article, published online on 28 March 2019 in Wiley Online Library (wileyonlinelibrary.com), and has been issued by agreement between the journal Editor-in-Chief, Robert Heath, and Wiley Periodicals LLC. The Expression of Concern has been agreed due to concerns raised by third parties. The article was found to present overstated and misleading conclusions regarding the identification of a putative novel macrophage subpopulation based on ZNF142 expression. In addition, the antibody reported to have been used for this identification was determined to be incorrect in the published article. In their subsequent clarification, the authors state that a different antibody was used (Prestige Antibodies, Sigma-Aldrich; catalog number HPA049594); however, this antibody is reported to be reactive against the human protein, and the study does not provide evidence validating its cross-species reactivity. Therefore, the journal has decided to issue an Expression of Concern to inform and alert the readers.</p>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"241 7","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13392841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}