journal of applied pharmaceutical science最新文献_第8页

Ethyl acetate crude fraction of Macaranga subpeltata and silymarin increase Vero cell survival and HepG2 cell death due to oxidative stress Macaranga subpeltata乙酸乙酯粗馏分和水飞蓟素提高Vero细胞存活率和HepG2细胞氧化应激死亡

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.84845

Harto Widodo, A. Rohman

{"title":"Ethyl acetate crude fraction of Macaranga subpeltata and silymarin increase Vero cell survival and HepG2 cell death due to oxidative stress","authors":"Harto Widodo, A. Rohman","doi":"10.7324/japs.2023.84845","DOIUrl":"https://doi.org/10.7324/japs.2023.84845","url":null,"abstract":"Reactive oxygen species (ROS) trigger oxidative stress (OS) that determines the onset of aging and various disease progressions including cancer. Macaranga subpeltata is among medicinal plants, which shows an important source of antioxidant (AO) compounds and has the potency to overcome those problems. Silymarin is a plant-derived flavonolignan with tremendous health benefits as an AO and organ protector. Hydrogen peroxide (H 2 O 2 ), an active prooxidant molecule, enables the generation of OS in mammalian cells. This study aims to evaluate the effect of ethyl acetate crude fraction of M. subpeltata (EACFM) and silymarin in H 2 O 2 -induced OS of Vero and HepG2 cell lines. The 3-(4,5-dimethyl azole-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate cell viability, while examination of ROS generation and cell death were done using the flow cytometric method. The study revealed that H 2 O 2 induced ROS generation in a dose-dependent manner both on Vero and HepG2 cell lines, and the HepG2 hepatocarcinoma cells were more resistant to H 2 O 2 toxicity than the Vero cells. Silymarin suppressed ROS formation on both H 2 O 2 -treated cell lines with different effects; it alleviated H 2 O 2 intoxication on Vero cells but enhanced the toxicity of H 2 O 2 on HepG2. The EACFM reduced H 2 O 2 intoxications of Vero cells and modulated ROS-mediated cell death of HepG2 cells through either apoptosis or necrosis. The EACFM showed a higher capacity to reduce OS due to H 2 O 2 treatment on Vero cells; however, silymarin showed better anticancer properties.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"132 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79617273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Level of antibiotic contamination in the major river systems: A review on South Asian countries perspective 主要河流系统中的抗生素污染水平:南亚国家视角的回顾

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.56748

P. Jassal, Dapinder Kaur, Manpreet Kaur, Pallavi Pallavi, Disha Sharma

引用次数: 0

In vitro antidiabetic and advanced glycation end products inhibitory activity of methanol extracts of various Dendrobium species 不同种类石斛甲醇提取物的体外抗糖尿病和晚期糖基化终产物抑制活性

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.75102

Thaniwan Cheun-Arom, B. Sritularak

{"title":"In vitro antidiabetic and advanced glycation end products inhibitory activity of methanol extracts of various Dendrobium species","authors":"Thaniwan Cheun-Arom, B. Sritularak","doi":"10.7324/japs.2023.75102","DOIUrl":"https://doi.org/10.7324/japs.2023.75102","url":null,"abstract":"Dendrobium orchids are one of the most varied species. In traditional Chinese medicine, Dendrobium has been reported to have an antidiabetic effect and has been used in the preparation of herbal medicines for a long time. This study aimed to investigate the in vitro antidiabetic (α-amylase and α-glucosidase assay) activities and advanced glycation end products (AGEs) inhibitory activity of the methanol extracts of 20 Dendrobium species. Phytochemical constituents and total phenolic and total flavonoid contents of the extracts were evaluated, as well. The total phenolic content was exhibited to be in the range of 31.73–132.70 mg GAE/g, and total flavonoid content was found in the range of 58.80–237.57 mg QE/g. The highest inhibitory activity of α-amylase was reported in D. parishii Rchb.f. (IC 50 46.57 µg/ml. The most effective inhibitory activity of α-glucosidase was found in D. ellipsophyllum Tang & Wang. (IC 50 128.69 µg/ml) and D. brymerianum Rchb.f. (IC 50 138.27 µg/ml). Dendrobium brymerianum Rchb.f. and D. scabrilingue Lindl. were shown to have the most potent inhibited AGEs formation with IC 50 values of 34.44 and 46.95 µg/ml, respectively, compared to aminoguanidine (IC 50 49.81 µg/ml). As a result, several Dendrobium species displayed strong antidiabetic and AGEs inhibitory activity, and they have the potential for further development.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80862093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological potency of actinomycetes extracts from rhizosphere soil of Dacrycarpus imbricatus from Toba Samosir, North Sumatra 北苏门答腊岛托巴萨摩泽根际土壤中放线菌提取物的生物效力

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.106853

Praptiwi Praptiwi, A. Fathoni, A. Putri, D. Wulansari, A. Agusta

引用次数: 0

Human platelet lysate-derived exosomes are superior to the lysate at increasing collagen deposition in a rat model of intrinsic aging 在大鼠固有衰老模型中，人血小板裂解液衍生的外泌体在增加胶原沉积方面优于裂解液

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.125707

Ervina Rosmarwati, Endra Yustin Ellistasari, A. Kusumawardani, Indah Julianto, S. Widhiati, N. A. Setyawan, Frieda Yanuar

{"title":"Human platelet lysate-derived exosomes are superior to the lysate at increasing collagen deposition in a rat model of intrinsic aging","authors":"Ervina Rosmarwati, Endra Yustin Ellistasari, A. Kusumawardani, Indah Julianto, S. Widhiati, N. A. Setyawan, Frieda Yanuar","doi":"10.7324/japs.2023.125707","DOIUrl":"https://doi.org/10.7324/japs.2023.125707","url":null,"abstract":"The decreased production of collagen in intrinsic aging skin is most likely due to downregulation of connective tissue growth factor (GF) which is thought to be a regulator of collagen expression. Human platelet lysate (HPL) contains many nutrients needed for cell growth and proliferation. The GFs are contained not right in liquid lysate but in exosomes. Our study demonstrated the superiority of Exo-HPL compared to HPL in decreasing matrix metalloproteinase (MMP)-1 level and increasing collagen deposition in intrinsic aging rat models (IRMs) induced by injection of D-galactose. The highest deposition of collagen at 40× magnification is found in IRM + Exo-HPL treatment group (38.40%) which is close to collagen deposition in young rats group (42.90%). At 100× magnification the study demonstrated that there was higher collagen deposition in IRM + Exo-HPL treatment group (39.94%) than in IRM + HPL treatment group (34.53%) which was close to the young rat group’s collagen deposition (43.26%). This study found a significant difference of MMP-1 level and collagen deposition among all groups. Both Exo-HPL and HPL treatment groups decreased MMP-1 level and increased collagen deposition compared to IRM group. Exo-HPL treatment group was more effective in decreasing MMP-1 level and increasing collagen deposition compared to HPL treatment group and IRM group significantly.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74256978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Identification of suitable flavonoids as insulin degrading enzyme inhibitors through in-silico approach 用硅法鉴定合适的类黄酮作为胰岛素降解酶抑制剂

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.137481

Saranya Dharmaraj, Preeya Negi, M. , Esakkimuthukumar, Akey Krishna Swaroop, S. Jubie

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Reduction of reproductive toxicity in murine sperm model using Moringa peregrina leaves extracts 辣木叶提取物对小鼠精子模型生殖毒性的影响

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.141064

Mohammed Abbas Mustafa Lali

引用次数: 0

Identification of flavone and flavanone from ethyl acetate fraction of Caesalpinia sappan heartwood and tentative structure-activity relationship: An in vitro and in silico approach 山参心材乙酸乙酯部位黄酮和黄酮的鉴定及构效关系的初步探讨:体外和室内研究

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.92856

Andrian Sucahyo, Moh. Farid Rahman, Husnul Khotimah, Widodo Widodo, Siti Mariyah Ulfa

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Chitosan-tripolyphosphate nanoparticles encapsulated rutin targeting bacterial growth inhibition and its cytotoxicity on PANC-1 pancreatic adenocarcinoma cell 壳聚糖-三聚磷酸纳米颗粒包膜芦丁抑制细菌生长及其对胰腺腺癌细胞PANC-1的细胞毒性

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.41561

K. T. R. Devi, Shalini James Paulraj, Yashesh Shah, Santhosh Kumar, Priyatharcini Kejamurthy

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Garcinia mangostana pericarp and α-mangostin normalized the expression of colonic and renal cytochrome P450 genes and abated the expression of renal inflammatory genes in dextran sulfate sodium-induced ulcerative colitis in mice 山竹果皮和α-山竹苷能使葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠结肠和肾脏细胞色素P450基因表达正常化，并抑制肾脏炎症基因的表达

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.132717

Naroeporn Nopwinyoowong, W. Chatuphonprasert, Nitima Tatiya- Aphiradee, K. Jarukamjorn

{"title":"Garcinia mangostana pericarp and α-mangostin normalized the expression of colonic and renal cytochrome P450 genes and abated the expression of renal inflammatory genes in dextran sulfate sodium-induced ulcerative colitis in mice","authors":"Naroeporn Nopwinyoowong, W. Chatuphonprasert, Nitima Tatiya- Aphiradee, K. Jarukamjorn","doi":"10.7324/japs.2023.132717","DOIUrl":"https://doi.org/10.7324/japs.2023.132717","url":null,"abstract":"Ulcerative colitis (UC) causes inflammation and ulceration in the colon and often develops renal manifestation. Garcinia mangostana pericarp crude extract (GM) and α-mangostin (MGS), a major bioactive compound, have potentiality to consider as a new complementary therapy for UC due to pharmacological activities, particularly anti-inflammatory activity. However, safety in terms of drug interaction has been neglected. The current study investigated the impacts of GM and MGS on the cytochrome P450 (CYP) profiles in the mouse colon and kidneys, including the inflammatory response in the kidneys. Male ICR mice were orally pre-treated with 40–1,000 mg/kg/day of GM, 30 mg/kg/day of MGS, or 100 mg/kg/day of sulfasalazine daily for 7 days. On days 4–7, 6 g/kg of 40 kDa dextran sulfate sodium (DSS) was orally administrated to induce UC. RT-qPCR was performed to determine the mRNA expressions of CYP and inflammatory genes. DSS suppressed Cyp1a1, Cyp2b9/10, Cyp2c29, Cyp2d9, Cyp2e1, Cyp3a11 , and Cyp3a13 expressions, whilst GM and MGS positively adjusted the CYP expression in both organs. Besides, Tnf-α, Mcp-1 , and Nf-κb expressions were up-regulated after UC induction, while they were restored to the level comparable to the control by GM and MGS. Therefore, G. mangostana pericarp is a promising candidate to normalize the CYP profiles and reducing the risk of drug interaction, and to revitalize against inflammation.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85356795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0