journal of applied pharmaceutical science最新文献_第9页

A sensitive direct chiral liquid chromatography tandem mass spectrometry method for the enantio—Selective analysis of imeglimin in formulation 一种灵敏的直接手性液相色谱串联质谱法用于制剂中依米霉素的对映体选择性分析

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.132349

Sangamithra Ramalingam, Meyyanathan Nainar Subramania, Babu Basuvan, R. Jaganathan, Anandha Jothi Dhavamani, N. K. Kandukuri, Ratnam Venkata Parimi, Sandeep Bodduna

{"title":"A sensitive direct chiral liquid chromatography tandem mass spectrometry method for the enantio—Selective analysis of imeglimin in formulation","authors":"Sangamithra Ramalingam, Meyyanathan Nainar Subramania, Babu Basuvan, R. Jaganathan, Anandha Jothi Dhavamani, N. K. Kandukuri, Ratnam Venkata Parimi, Sandeep Bodduna","doi":"10.7324/japs.2023.132349","DOIUrl":"https://doi.org/10.7324/japs.2023.132349","url":null,"abstract":"The purpose of this study was to separate and develop a sensitive chiral liquid chromatography tandem mass spectrometry (LC-MS/MS) technique to estimate the (+) and (−) enantiomers of imeglimin in its formulation and validate individual enantiomer of the drug. Imeglimin is used to treat type-2 diabetes (T2D). The chiral stationary phase was reverse phase Chiralpak IG-3 (100 × 4.6 mm, 3 µm), whereas the isocratic mobile phase was methanol and 10 mM ammonium acetate (95:5 v / v ratio) and the flow rate was 0.5 ml/minutes. The resolution of the (+) and (−) enantiomers was monitored using LC-ESI-MS/MS in positive transition at 155.0 m / z (M+H) for imeglimin. The (+) and (−) linearity ranges from 10 to 100 ng/ml. The correlation co-efficient ( R 2 ) of the (+) and (−) imeglimin enantiomers was found to be linear. The retention time of the (+) and (−) enantiomers of the drug was 2.876 and 4.325 minutes and the total run time of the chromatographic resolution was 5 minutes. As a result, our goal is to separate the enantiomers and develop a fast, sensitive, and cost-effective method for estimating (+) and (−) enantiomers in its formulation.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90762235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endophytic fungus isolated from Zingiber officinale Linn. var. rubrum as a source of antimicrobial compounds 生姜内生真菌的分离。作为抗菌化合物的来源

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.134154

D. Handayani, Hardieta Citra Sari, E. Julianti, M. A. Artasasta

{"title":"Endophytic fungus isolated from Zingiber officinale Linn. var. rubrum as a source of antimicrobial compounds","authors":"D. Handayani, Hardieta Citra Sari, E. Julianti, M. A. Artasasta","doi":"10.7324/japs.2023.134154","DOIUrl":"https://doi.org/10.7324/japs.2023.134154","url":null,"abstract":"In Indonesia, red ginger ( Zingiber officinale Linn. var. rubrum ) is an essential traditional medicine used to treat various ailments. Red ginger plants may have an endophytic fungus capable of producing several bioactive chemicals. This research aimed to identify endophytic fungi and evaluate their antimicrobial efficacy. The fungal strains were isolated from red ginger leaves, stems, and rhizomes by direct planting and pour methods using fungus culture medium and cultivated on rice medium. In the secondary metabolite extraction process, ethyl acetate (EtOAc) is used as a solvent. The agar diffusion procedure examined the EtOAc extract for antibacterial activity against Staphylococcus aureus , Escherichia coli , and Candida albicans . This study succeeded in isolating 10 fungal strains. The result of antimicrobial screening revealed that the fungal strain JMR4 has a high ability to suppress the development of pathogenic bacteria S. aureus and E. coli , with an inhibition zone of 14.38 ± 1.34 and 16.88 ± 0.69 mm, respectively. The fungus was later identified using the molecular approach, which stated that this fungus is Aspergillus terreus . This research demonstrates that endophytic fungus on red ginger plants may synthesize antibacterial substances crucial for developing novel antibiotics.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89828654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation on antioxidative and neuroprotective activity of bacoside- A, Asiatic acid and kaempferol in endothelin-1 induced cerebral ischemia in rat 马齿苋苷- A、亚补酸和山奈酚对内皮素-1所致大鼠脑缺血的抗氧化和神经保护作用

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.153365

Ashutosh Ghosh, Nasima Khanam, D. Nath

引用次数: 0

Isorhamnetin decreased the expression of HMG-CoA reductase and increased LDL receptors in HepG2 cells 异鼠李素降低HepG2细胞中HMG-CoA还原酶的表达，增加LDL受体的表达

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.103374

Randa El- Rayyes, M. Abbas, Razan Obeidat, M. Abbas

{"title":"Isorhamnetin decreased the expression of HMG-CoA reductase and increased LDL receptors in HepG2 cells","authors":"Randa El- Rayyes, M. Abbas, Razan Obeidat, M. Abbas","doi":"10.7324/japs.2023.103374","DOIUrl":"https://doi.org/10.7324/japs.2023.103374","url":null,"abstract":"Isorhamnetin is a flavonoid present in many plants. In a previous in vivo study, isorhamnetin lowered serum cholesterol of rats fed high-cholesterol diet. However, its mechanism of action was not investigated. In the present work, the mechanism of its hypocholesterolemic effect was studied. The expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) and low-density lipoprotein receptor (LDLR) genes and proteins was studied in HepG2 liver cancer cell line by polymerase chain reaction, western blot, and indirect enzyme-linked immunosorbent assay as well as its antioxidant activity. sorhamnetin had an IC 50 of 100, 53, and 40 µM at 24, 48, and 72 hours, respectively, in HepG2 cells. Isorhamnetin downregulated HMG-CoA reductase gene expression significantly. Also, all tested doses of isorhamnetin downregulated LDLR expression and produced no change in membranous LDLR protein expression. In cell lysate, LDLR was increased by all studied concentrations of isorhamnetin. Isorhamnetin (100 µM) decreased intracellular HMG-CoA reductase compared to vehicle-treated control. Furthermore, isorhamnetin increased superoxide dismutase activity and reduced H 2 O 2 level, due to catalase activity. Isorhamnetin reduced HMG-CoA reductase gene expression and increased total LDLR and exerted pronounced antioxidant action.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90985991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The role of catechins of Camellia sinensis leaves in modulating antioxidant enzymes: A review and case study 茶树叶儿茶素在抗氧化酶调节中的作用:综述与实例研究

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.143056

Lidya Cahyo Bawono, M. A. Khairinisa, Supat Jiranusornkul, J. Levita

{"title":"The role of catechins of Camellia sinensis leaves in modulating antioxidant enzymes: A review and case study","authors":"Lidya Cahyo Bawono, M. A. Khairinisa, Supat Jiranusornkul, J. Levita","doi":"10.7324/japs.2023.143056","DOIUrl":"https://doi.org/10.7324/japs.2023.143056","url":null,"abstract":"Free radicals are generated in the body due to pollution and unhealthy lifestyle. Unbalanced levels of free radicals and natural antioxidants in the body may induce oxidative stress (OS). OS is responsible for several illnesses, including diabetes mellitus, cancer, and cardiovascular disease. The antioxidants can potentially increase the defense mechanism against OS and protect human health. The study aimed to analyze the catechins’ role as antioxidants. The catechins can upregulate antioxidant enzymes, scavenge free radicals, protect the DNA by intercalating to the helixes, and create chelation due to numerous hydroxyl moieties attached to the aromatic ring, which protects the structural integrity through electron delocalization. Epigallocatechin gallate (EGCG), a catechin with the greatest antioxidant capacity, has ortho-phenolic hydroxyl groups which potentially in binding free radicals. Moreover, the case study showed that dose-dependent treatment of tea had some benefits for human health. Humans with anemia and menopause cannot consume tea in high doses, which could worsen the condition. Conversely, humans with thalassemia are suggested to take tea to decrease the iron in their bodies. This review is expected to be a further study reference, mainly to clarify the EGCG process in restoring antioxidant enzymes and activating the thioredoxin antioxidant system.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90127204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational simulations of microalgae-derived bioactive compounds as a novel inhibitor against B-Raf V600E-driven melanoma 微藻衍生生物活性化合物作为B-Raf v600e驱动的黑色素瘤新抑制剂的计算模拟

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.10012

F. Prasetiya, Wanda Destiarani, Irene Retno Cahya Prihastaningtyas, Mochamad Untung Kurnia Agung, Muhammad Yusuf

{"title":"Computational simulations of microalgae-derived bioactive compounds as a novel inhibitor against B-Raf V600E-driven melanoma","authors":"F. Prasetiya, Wanda Destiarani, Irene Retno Cahya Prihastaningtyas, Mochamad Untung Kurnia Agung, Muhammad Yusuf","doi":"10.7324/japs.2023.10012","DOIUrl":"https://doi.org/10.7324/japs.2023.10012","url":null,"abstract":"Melanoma is one of the most aggressive types of cancer, which has shown a tremendous surge in the last 50 years. Therapy for advanced-type melanoma is still a challenge because of the low response rate and 10-year survival. Therefore, drug discovery efforts need to be made to fight this cancer. To date, the development of big data and 3D has made it easier for researchers to understand the structure of proteins or enzymes that play an important role as receptors in melanoma cancer to be used as specific targets for diagnosis and therapy, for instance, the B-Raf V600E. This study examined the potential of active compounds from microalgae for developing melanoma anticancer drugs. The database was constructed using data mining from MarinLit and the related publications from 1970 to 2020. In silico methods such as molecular docking, virtual screening, and molecular dynamic simulations were used to find the most potential candidates. A total of 25 compounds passed the virtual screening stage. The top three compounds based on the binding free energy compared to a natural ligand and commercial drug are cholesta-5,7-dien-3beta-ol, 24-oxocholesterol acetate, lathosterol, and two additional compounds, phycocyanin and phycocyanobilin, were also selected due to their massive production from the most commonly cultured microalgae worldwide, Arthrospira sp. (previously known as Spirulina sp.). Furthermore, ADME analysis and toxicity tests were also carried out. Molecular dynamics simulation showed that phycocyanin was the best potential candidate for melanoma anticancer drugs, with free binding energies ranging from −65 to −80 kcal/mol. This result was also supported by root mean square deviation, root mean square fluctuation, and distance parameter data. This study may accelerate molecular research in producing therapeutic compounds for melanoma cancer, thus allowing it to continue developing pharmaceutical products that benefit human health.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79265357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-based hydrotalcite nanostructured membranes containing sodium alendronate for guided bone regeneration therapy 含阿仑膦酸钠的壳聚糖基水滑石纳米膜用于骨再生引导治疗

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.40064

Fábio Luiz Costa de Souza, Alice Simon, Evandro Lúcio Barcelos D’Ornellas, Raphael Ferreira da Silva, Ana Carolina Mattos Covas, C. R. Rodrigues, Luiz Cláudio Rodrigues Pereira da Silva, F. A. Carmo, L. Cabral

{"title":"Chitosan-based hydrotalcite nanostructured membranes containing sodium alendronate for guided bone regeneration therapy","authors":"Fábio Luiz Costa de Souza, Alice Simon, Evandro Lúcio Barcelos D’Ornellas, Raphael Ferreira da Silva, Ana Carolina Mattos Covas, C. R. Rodrigues, Luiz Cláudio Rodrigues Pereira da Silva, F. A. Carmo, L. Cabral","doi":"10.7324/japs.2023.40064","DOIUrl":"https://doi.org/10.7324/japs.2023.40064","url":null,"abstract":"Guided bone regeneration is commonly applied in clinical dental practice for alveolar bone reconstruction. This work aims to develop nanostructured membranes with chitosan, hydrotalcite, and sodium alendronate for guided bone regeneration. Low, medium, and high molecular weight chitosan membranes were prepared to contain hydrotalcite or nanoparticulate hydrotalcite and sodium alendronate added directly in the polymer matrix or interlayered between clay lamellae. The membranes were analyzed for nanocomposite formation by X-ray diffraction and differential scanning calorimetry analyses, and water absorption and tensile strength were also assessed. In vitro assays using Saos-2 cells checked the biocompatibility, cell viability, and osteoinduction potential of the membranes. The chitosan and hydrotalcite physical mixture membrane series did not form nanocomposites and collapsed in the water absorption test. Membranes with nanoparticulate hydrotalcite with sodium alendronate interspersed formed nanocomposites and showed a good profile of water absorption and tensile strength. All nanocomposite membranes were biocompatible and demonstrated potential for osteoinduction in vitro . The membranes of the nanoparticulate hydrotalcite series presented the best performance in the in vitro assays, especially the membrane formed with low and medium molecular weight chitosan, representing a potential new therapy for guided bone regeneration, which should be tested in vivo in the near future.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74269367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Traditional Thai herbal medicine for treating COVID-19 治疗COVID-19的传统泰国草药

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.126979

Phayong Thepaksorn, Katanchalee Thabsri, Piyachat Evelyn Denbaes, Suwannee Sroisong, Pussadee Srathong, Suriyan Sukati

引用次数: 0

Response surface modeling to optimize sonication extraction with the maceration method for the phenolic content and antioxidant activity of Justicia gendarussa Burm f. 利用响应面建模优化浸出法提取龙胆中酚类物质含量及抗氧化活性。

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.143746

Waras Nurcholis, Mega Safithri, Nelly Marliani, Muhammad Iqbal

引用次数: 1

Network pharmacology study of Phyllanthus niruri: Potential target proteins and their hepatoprotective activities 余甘子网络药理学研究:潜在靶蛋白及其保肝活性

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.146937

Santi Tan, Adi Yulandi, Raymond Rubianto Tjandrawinata

引用次数: 0