journal of applied pharmaceutical science最新文献

Fabrication of carotenoid enriched Crocus sativus w/o nanoemulsion: Optimization and pharmaceutical characterization 制备富含胡萝卜素的十字花科植物含氧纳米乳液：优化和药物表征

journal of applied pharmaceutical science Pub Date : 2024-04-05 DOI: 10.7324/japs.2024.158623

N. Parikh, Komal Patel, A. Dharamsi

{"title":"Fabrication of carotenoid enriched Crocus sativus w/o nanoemulsion: Optimization and pharmaceutical characterization","authors":"N. Parikh, Komal Patel, A. Dharamsi","doi":"10.7324/japs.2024.158623","DOIUrl":"https://doi.org/10.7324/japs.2024.158623","url":null,"abstract":"Herbal-based preparations have progressively attracted more interest nowadays due to multiple therapeutic approaches. Major concerns with the use of herbal preparations and their application as functional ingredients are about their stability, solubility, and bioaccessibility. Crocus sativus is one of the crucial nootropic traditional herbs, rich in potential phytochemicals used for the treatment of long-standing disorders. Many studies have suggested that the bioactive present in C. sativus extract may undergo enzymatic degradation in the intestine and hence less bioavailable at the site of action. A novel drug delivery approach to herbal preparation may combat the associated challenge. The present research focuses on the feasibility of developing w/o nanoemulsion incorporating the carotenoid-enriched C. sativus extract by spontaneous emulsification method. Critical process variables were optimized using 32 factorial design with Design of Expert software. The optimized formulation of w/o nanoemulsion comprising 9 mg/ml of C. sativus extracts with 12% internal phase, 21% S-mix, and 67% oil phase produced a globule size of 94.34 nm. The total content present in the nanoemulsion was 83% bioactive determined by the High Performance Liquid Chromatography- Photo Diode Array (HPLC-PDA) method. Further characterization, the Transmission Electron Microscopy (TEM) images showed the overall nanoemulsion system morphology with uniform distribution of globules of the internal phase. The in vitro release of bioactives from developed nanoemulsion showed an initial burst release of 60% at 1 hour following slow drug release. Results demonstrated that w/o nanoemulsion of C. sativus extract overcome the associated challenges and may reserve its space for medicinal application.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"31 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring carp scales (Cyprinus carpio L.) as a novel source of collagen for dental use: Extraction and characterization 探索将鲤鱼鳞片（Cyprinus carpio L.）作为牙科用胶原蛋白的新来源：提取和表征

journal of applied pharmaceutical science Pub Date : 2024-04-05 DOI: 10.7324/japs.2024.166542

D. Carolina, M. Satari, B. Priosoeryanto, Agus Susanto, C. Sukotjo, R. E. Kartasasmita

{"title":"Exploring carp scales (Cyprinus carpio L.) as a novel source of collagen for dental use: Extraction and characterization","authors":"D. Carolina, M. Satari, B. Priosoeryanto, Agus Susanto, C. Sukotjo, R. E. Kartasasmita","doi":"10.7324/japs.2024.166542","DOIUrl":"https://doi.org/10.7324/japs.2024.166542","url":null,"abstract":"Previous studies have shown that using bovine and pork collagen in dentistry is associated with various limitations, such as bovine spongiform encephalopathy, transmissible spongiform encephalopathy, and foot and mouth disease. This study aims to examine the extraction and characterization of collagen from common carp (Cyprinus carpio L.) scales, as an alternative source in dentistry. The samples used in this study consisted of common carp (C. carpio L.) scales. In the first step, common carp scales were acid-extracted and spray-dried to isolate collagen. After the collagen had been isolated, it was tested for moisture content, ash, acidity, and functional groups, with Indonesian National Standard (SNI) 8076:2014 as the reference standard. The characterization parameters of the common carp scale collagen powder revealed an 11% yield, 3.66% moisture content, 0.32% ash content, and a pH of 7.38. Furthermore, fourier transform infrared spectroscopy spectra confirmed the presence of a triple helix structure. The extraction of collagen from common carp scales using the acetic acid method and spray drying technique successfully adhered to the composition standards specified by the SNI 8076:2014. Based on these findings, common carp scales could be considered a viable collagen replacement source for applications in dentistry.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"37 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140737487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of polyprotein and 3D polymerase protein in Sf9 cells and immunogenicity against enterovirus A71B5 (Thailand strain) 多蛋白和3D聚合酶蛋白在Sf9细胞中的表达及对肠病毒A71B5(泰国株)的免疫原性

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.93192

Nipatha Issaro, Aphisak Kongkaew, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, W. Nimlamool, M. N. Takuathung

{"title":"Expression of polyprotein and 3D polymerase protein in Sf9 cells and immunogenicity against enterovirus A71B5 (Thailand strain)","authors":"Nipatha Issaro, Aphisak Kongkaew, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, W. Nimlamool, M. N. Takuathung","doi":"10.7324/japs.2023.93192","DOIUrl":"https://doi.org/10.7324/japs.2023.93192","url":null,"abstract":"Enterovirus 71 B5 subgenotype (EV-A71B5) is a primary human pathogen of hand, foot, and mouth disease. In this study, we aimed to prepare a novel recombinant protein polyprotein (P1) and 3D polymerase (3D p ° l ) protein of EV-A71B5 and determine mice immunogenicity and neutralization activity against EV-A71 of the B5 subgenotype commonly found in Thailand. Using a dual promoter system and investigating its expression in Sf9 cells, we constructed a novel recombinant protein containing P1 and 3D p ° l protein. The purified P1-3D p ° l was observed by western blotting and transmission electron microscopy (TEM) to determine the particle size. Furthermore, we determined the immunogenicity and neutralization activity against EV-A71 of the B5 subgenotype using concatenation of Bagg and Albino (BALB/c) mice. The results revealed that P1-3D p ° l was expressed in Sf9 cells. We used TEM to visualize the particle size of P1-3D p ° l to be approximately 33 nm. P1-3D p ° l had the potential to elicit the production of immunoglobulin G and immunoglobulin M antibodies and the T helper type 1-dominant cytokine interferon-γ after immunizing BALB/c mice and inducing neutralization antibodies against EV-A71B5. Our results demonstrated that Sf9 cells successfully produced P1-3D p ° l , which can leverage immune efficacy in BALB/c mice and be used to develop vaccines against the EV-A71B5 strain prevalent in Thailand.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75432433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of health-related quality of life and its contributing factors using the WHOQOL-BREF questionnaire in patients with chronic liver disease 使用WHOQOL-BREF问卷评估慢性肝病患者健康相关生活质量及其影响因素

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.120282

Manjacheri Kuppadakath Sagara, Vineesha Gangadharan, Ranjith Sristhi, B. Shaji, Adithi Kellarai, J. Joel

{"title":"Assessment of health-related quality of life and its contributing factors using the WHOQOL-BREF questionnaire in patients with chronic liver disease","authors":"Manjacheri Kuppadakath Sagara, Vineesha Gangadharan, Ranjith Sristhi, B. Shaji, Adithi Kellarai, J. Joel","doi":"10.7324/japs.2023.120282","DOIUrl":"https://doi.org/10.7324/japs.2023.120282","url":null,"abstract":"Chronic liver disease (CLD) is a serious condition characterized by a progressive decline in liver functions, which leads to impaired quality of life (QOL). A prospective cross-sectional study was conducted with 165 patients to identify the health-related QOL in CLD, for 6 months. The QOL of CLD patients was assessed using the World Health Organization quality of life-BREF questionnaire and the severity of the disease was assessed using Child-Pugh Model for End-Stage Liver Disease (MELD) score. The incidence of CLD was found to be high in males (89.09%). While assessing, the overall QOL was identified as 50.96 ± 15.77. The highest score was observed in the social relationships domain (58.33 ± 19.34) followed by environment (54.74 ± 16.74), psychological (46.70 ± 19.81), and physical health (44.08 ± 18.64). The socio-demographic factors associated with QOL include body mass index, education, alcohol consumption, smoking, and tobacco. In the Child-Pugh MELD Score, the QOL was higher in class A (Good hepatic function) with a MELD score of 6–9 (1.9% mortality), the mean score being 60 ± 21 in contrast with the other classes. From the study, it is evident that CLD has a significant impact on the QOL. Patient-centric collaborative care, including physicians, nurses, pharmacists, and other health care providers are essential to improve the QOL.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73868901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of Clausena lansium (Lour.) peel extract consumption on hyperglycemia, hyperlipidemia and augmented oxidative stress in type-2 diabetic model rats Clausena lanum (Lour.)皮提取物对2型糖尿病模型大鼠高血糖、高脂血症和氧化应激增强的影响

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.112499

W. Phachonpai

{"title":"The potential of Clausena lansium (Lour.) peel extract consumption on hyperglycemia, hyperlipidemia and augmented oxidative stress in type-2 diabetic model rats","authors":"W. Phachonpai","doi":"10.7324/japs.2023.112499","DOIUrl":"https://doi.org/10.7324/japs.2023.112499","url":null,"abstract":"Epidemiological studies have shown that the consumption of some plant components can combat type 2 diabetes mellitus (T2DM) and its complications through various biological properties such as antioxidant activity. The extract of wampee peel (WP) exhibits several medical properties, especially antioxidant effects. We aimed to explore the antihyperlipidemic, hypoglycemic, and antioxidant effects of WP peel extract in an experimental T2DM rat model. T2DM was induced in the experimental groups by a high-fat diet and streptozotocin injection (HFD/STZ). Rats with a fasting blood glucose level above 200 mg/dl were considered diabetic and were separated into six groups (control, diabetic, diabetic treated with WP peel extract at 200, 400, or 600 mg/kg, and diabetic treated with metformin at 250 mg/kg). After 4 weeks of treatment, blood samples were collected for analysis. HFD/STZ-induced rats exhibited hyperglycemia, dyslipidemia, increased HbA1c, and increased lipid peroxidation, but reduced serum antioxidant enzyme activities. However, these changes were attenuated in T2DM rats with the highest dose of WP peel extract. Our results provide strong evidence that WP peel extract has antihyperlipidemic, antidiabetic, and antioxidant properties and thus also suggest that WP peel extract may be useful in developing novel agents for the management of T2DM.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74185048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A qualitative exploration of children’s willingness to take the COVID-19 vaccine in Jordan 约旦儿童接种COVID-19疫苗意愿的定性探讨

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.54481

T. Mukattash, K. Kheirallah, Hadeel Daradkeh, A. Jarab, Rana K. Abu‐Farha, Mohammad B. Nusair, Samar Karout, Rania Itani

{"title":"A qualitative exploration of children’s willingness to take the COVID-19 vaccine in Jordan","authors":"T. Mukattash, K. Kheirallah, Hadeel Daradkeh, A. Jarab, Rana K. Abu‐Farha, Mohammad B. Nusair, Samar Karout, Rania Itani","doi":"10.7324/japs.2023.54481","DOIUrl":"https://doi.org/10.7324/japs.2023.54481","url":null,"abstract":"Vaccine willingness has an important role in COVID-19 adult vaccination programs in many countries. However, there are limited data about vaccine willingness among children, which is largely confined to parents' opinions. With the emergence of new variants affecting this age group, children's opinions on COVID vaccination are important. This study aimed to explore children's willingness and opinions regarding COVID-19 vaccination. Focus group discussions, including children, were carried out in different Jordanian schools. Purposive sampling was implemented to select students of different socioeconomic statuses. A total of 54 school students aged 6–14 years from three schools participated in nine focus groups. Overall, participating children were willing to take the COVID-19 vaccine, and three overarching themes described their view regarding the vaccine: benefits, motivators, and hesitancy and barriers. Participating children reported that the vaccine could be beneficial as it protects them from being infected, and they are motivated to take it as it has been approved for children in other countries. However, some children were hesitant regarding taking the vaccine as it could be harmful, and they believed that COVID-19 is not severe in children. In conclusion, this study shows a clear willingness among participants from primary and secondary schools to take the COVID-19 vaccine. Such outcomes should be tested further on a larger scale to deliver future recommendations to include younger children in the vaccination program © 2023 Tareq L. Mukattash et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84286167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combretum hypopilinum exhibited anti-inflammatory activity in LPS-stimulated RAW 264.7 murine macrophages through inhibition of inflammatory mediators and apoptosis Combretum hypoopilinum通过抑制炎症介质和细胞凋亡，对lps刺激的小鼠巨噬细胞表现出抗炎活性

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.119177

K. Idoh, K. Dosseh, Mrinmoy Gautam, Tchazou Kpatcha, N. Manikandan, A. Agbonon, M. Ramanathan, M. Gbéassor

引用次数: 0

Tetrahydrobisbenzylisoquinoline alkaloids from Phaeanthus ophthalmicus inhibit target enzymes associated with type 2 diabetes and obesity 四氢双苄基异喹啉生物碱可抑制与2型糖尿病和肥胖相关的靶酶

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.154518

J. A. Manzano, L. C. Llames, A. Macabeo

{"title":"Tetrahydrobisbenzylisoquinoline alkaloids from Phaeanthus ophthalmicus inhibit target enzymes associated with type 2 diabetes and obesity","authors":"J. A. Manzano, L. C. Llames, A. Macabeo","doi":"10.7324/japs.2023.154518","DOIUrl":"https://doi.org/10.7324/japs.2023.154518","url":null,"abstract":"Diabetes and obesity are metabolic comorbidities declared by WHO as epidemics. Owing to the purported pharmaceutical activities of plant-derived secondary metabolites, we assessed the inhibitory potentials of the Philippine native plant Phaeanthus ophthalmicus tetrahydrobisbenzylisoquinoline alkaloidal constituents tetrandrine ( 1 ) and limacusine ( 2 ) against enzymes implicated in type 2 diabetes (T2D) and obesity such as α-glucosidase, dipeptidyl peptidase-IV (DPP-IV), porcine pancreatic lipase (PPL), and human monoacylglycerol lipase (MAGL) using in vitro experiments and molecular docking. Both alkaloids 1 (IC50 = 2.29 μg/ml) and 2 (IC50 = 2.68 μg/ml) showed stronger inhibition against α-glucosidase compared to the drug control acarbose (IC50 = 4.12 μg/ml). Alkaloids 1 (IC50 = 4.92 μg/ml) and 2 (IC50 = 3.80 μg/ml) also exhibited better inhibitory activities against DPP-IV compared to the drug control sitagliptin (IC50 = 6.90 μg/ml). Molecular docking results revealed better binding propensities for both 1 and 2 onto the active pocket of α-glucosidase and DPP-IV compared to their respective control drugs. Meanwhile, alkaloid 2 showed better in vitro (IC 50 = 0.70 μg/ml) and in silico inhibitory activity vs PPL compared to orlistat. Both alkaloids 1 and 2 showed moderate bioactivity against MAGL. Both alkaloids were predicted to possess drug-likeness properties. Our present study suggests the potentials of the tetrahydrobisbenzylisoquinoline alkaloidal phytoconstituents tetrandrine ( 1 ) and limacusine ( 2 ) from P. ophthalmicus in developing new-generation prodrugs against T2D and obesity.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80871196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of potential bioactive compounds from Azadirachta indica (Neem) as inhibitors of SARS-CoV-2 main protease: Molecular docking and molecular dynamics simulation studies 印楝作为SARS-CoV-2主要蛋白酶抑制剂的潜在生物活性化合物鉴定:分子对接和分子动力学模拟研究

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.139799

Donny Ramadhan, Firdayani Firdayani, Nihayatul Karimah, Elpri Eka Permadi, Sjaikhurrizal El Muttaqien, Agus Supriyono

引用次数: 0

Computational design for the development of natural molecules as compelling inhibitors against the target SARS-CoV-2: An in-silico attempt 开发天然分子作为靶向SARS-CoV-2的引人注目的抑制剂的计算设计:计算机尝试

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI: 10.7324/japs.2023.134301

Nagarjuna Palathoti, Kalirajan Rajagopal, Gowramma Byran, Kannan Raman, Edwin Jose, Manikandan Gurunathan

引用次数: 0