journal of applied pharmaceutical science最新文献

{"title":"Stability study of spray freeze-dried insulin dry powder formulations used for nose-to-brain delivery","authors":"Cynthia Marisca Muntu, Christina Avanti, Hayun Hayun, Silvia Surini","doi":"10.7324/japs.2023.148983","DOIUrl":"https://doi.org/10.7324/japs.2023.148983","url":null,"abstract":"Formula Optimization of Rifampicin Dry Powder Inhalation with Chitosan-Xanthan Carrier Using Response Surface MethodologySilvia Surini, Rahmadina Providya, Kurnia Sari Setio Putri","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135784092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary hypothetical assessment and in silico molecular docking of statin to VEGFR2 and VEGFR3 protein complex associated with angiogenesis and lymphangiogenesis in diabetic wounds","authors":"Divya Pamu, Selvaraj , Ayyamperumal, Munikumar Manne, Vyshnavi Tallapaneni, Chandrasekar Joghee Nanjan Moola, Veera Venkata Satyanarayana Reddy Karri","doi":"10.7324/japs.2023.118396","DOIUrl":"https://doi.org/10.7324/japs.2023.118396","url":null,"abstract":"In silico analysis of phytochemical compounds for optimizing the inhibitors of HMG CoA reductaseJ. Mercy Jasmine and R. Vanaja","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135550541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Various innovative UV spectroscopic methodologies for concurrent estimation of dapagliflozin and vildagliptin in combined tablet","authors":"A. Sen, Satish B. Khatariya, D. Sen, A. Zanwar, Rajesh A. Maheshwar, Ramaswamy Velmurugan","doi":"10.7324/japs.2023.151424","DOIUrl":"https://doi.org/10.7324/japs.2023.151424","url":null,"abstract":"Improved glycemic regulation in people with diabetes mellitus can be achieved with a fixed-dose combination (tablets) comprising dapagliflozin 10 mg and vildagliptin 100 mg. The proposed research suggests five spectrophotometric methodologies namely simultaneous equation, absorbance ratio, second derivative zero crossing, ratio difference, and first derivative of ratio spectra methods for the simultaneous assessment of the combined tablet that are straightforward, fast, easy, accurate, and reproducible. The concentration series for DPZ showed a strong linear correlation between 0.5 and 10 µg/ml for the first, second, third, and fourth methods and between 1 and 15 µg/ml for the fifth method. However, VGT displayed exceptional linear association in the sequence of 5–100 µg/ml for the first, second, third, and fourth methods; 10–150 µg/ml for the fifth method. The outcome of precision studies was evaluated in terms of % RSD, following International Conference on Harmonization guideline acceptable limits (˂2), which shows good repeatability, low intra, and interday variability, indicating an excellent precision of the developed methods. The outcome of recovery studies ranged from 96% to 103% for both the drug suggests the suitability of the proposed methods. Percentage recovery indicates that there was no interference from tablet excipients. Moreover, the low limit of detection and the limit of quantification values prove the sensitivity of the proposed methods. The projected methods were successfully applied for the quantitative determination of both drugs. Sample solutions were analyzed six times and experimental values were found to be within 98% and 101% for both the drugs. Proposed methods were compared with reported methods in terms of their name of methods, range, sensitivity, specificity, solvents used, and application. The proposed methods are found to be comparable with the reported methods and can cover up shortcomings and thus can be utilized as alternative methods for the simultaneous assessment of dapagliflozin and vildagliptin in the combined formulation.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78106940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of turmeric (Curcuma longa) extract administration on tumor necrosis factor-alpha and type 1 collagen expression in UVB-light radiated BALB/c mice","authors":"Aletheia Threskeia, Willy Sandhika, R. Rahayu","doi":"10.7324/japs.2023.19358","DOIUrl":"https://doi.org/10.7324/japs.2023.19358","url":null,"abstract":"Skin aging caused by chronic exposure to ultraviolet is called photoaging. Ultraviolet B (UVB) is also known to cause skin cancer. On the other hand, curcumin, an active component found in turmeric ( Curcuma longa ), is a potent antioxidant. Herein, with rising skin cancer rates and interest in preventing photoaging, this study aims to analyze the photoprotective potential of topical turmeric extract with pharmacological activity against UVB. This experiment used 32 BALB/c mice, and the mice were randomly divided into 4 groups: normal control group (NC), sick control/ UVB-exposed group (SC), UVB-exposed + vehicle group (VT), and UVB exposed + topical turmeric extract group (TT). Except for group NC, all groups received UVB radiation 6 times/week for 4 weeks (24 times in total). At the end of the treatment, the skin tissues were examined using immunohistochemistry. From the interpretation of immunohistochemistry results, there was a significant decrease in tumor necrosis factor expression and an increase in type 1 collagen for TT compared to the control ( p < 0.05). In conclusion, the C. longa might have a potential photoprotector property in preventing photoaging.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74744666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ficus exasperata leaves aqueous extract influences pathophysiologic mechanisms of diabetic peripheral neuropathy in a rat model","authors":"HidaayahO Jimoh-Abdulghaffaar, A. Ajibare, O. Akintoye, O. Jimoh, Evelyn Nnashiru Ananias, Joshua Owoyemi, Victor Oluwatoju Ibiyeye, L. Ojulari","doi":"10.7324/japs.2023.98174","DOIUrl":"https://doi.org/10.7324/japs.2023.98174","url":null,"abstract":"Aqueous extract of Ficus exasperata leaves was investigated for its antinociceptive and anti-oxidant activities in streptozocin (STZ)-induced diabetic peripheral neuropathic rats. 36 male albino rats weighing between 120 and 170 g were randomly assigned into four groups of nine animals each. Rats in group 1 (non-diabetic) received normal saline 1.0 ml/kg b.w., group 2 (diabetic untreated) received normal saline 1.0 ml/kg b.w., group 3 were treated with pregabalin 0.71 mg/kg b. w. and group 4 were treated with F. exasperata 200 mg/kg b. w. following onset of peripheral neuropathy after induction of diabetes, rats received drug treatment orally, once daily for 21 days. They were then sacrificed ad blood, brain, skin, and sciatic nerve samples were collected. The plasma was assayed for glutathione and superoxide dismutase, the brain was assayed for malondialdehyde and nitric oxide, and the skin was assayed for Calcitonin gene-related peptide and Substance P, while the sciatic nerve was assayed for brain-derived neurotrophic factor and nerve growth factor. Pain threshold was assessed by testing for thermal allodynia using ice cold and hot plate tests. Aqueous leaf extract of F. exasperata reversed hyperglycemia, allodynia, and oxidative stress induced by STZ.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"49 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72449878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational approach to determine the combination of polyherbs based on the interaction of their metal complexes on the mucoadhesive properties of type II mucin","authors":"E. D. P. Lestari, S. Widyarti, Djoko Herry Santjojo, N. Widodo, S. Sumitro","doi":"10.7324/japs.2023.35062","DOIUrl":"https://doi.org/10.7324/japs.2023.35062","url":null,"abstract":"Mucin is the main mucus component besides water, which comes from the glycoprotein group. Mucoadhesive properties can be analyzed based on the binding interactions between compounds and mucin in mucus. This research is an in-silico approach aiming to obtain bioactive compounds that have a stable interaction with mucin-type II (MUC2) by screening ten compounds used in Indonesian herbal medicines. Molecular docking was used as a procedure for forming the compound-mucin complexes. Furthermore, molecular dynamic simulation with YASARA Dynamics software was used to obtain the stability of compound-mucin complexes. The curcumin-metal ions and kaempferol-metal ions complexes showed the most stable binding affinity and interaction with MUC2 compared to eight other ligands. Based on the molecular dynamic simulation, kaempferol-metal ions complexes are predicted to interact in the D3 chain, while curcumin-metal ions complexes more stably interact with the CysD1 chain. Both complex interactions simultaneously strengthen and synergize interactions with MUC2. Herbs containing one or both bioactive compounds, curcumin, and kaempferol are supposed to be composed into a polyherbal composition to obtain optimal mucoadhesive properties for MUC2","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84791056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput RNA extraction method for P. aeruginosa and S. pyogenes biofilms","authors":"Walid Aburayyan","doi":"10.7324/japs.2023.28606","DOIUrl":"https://doi.org/10.7324/japs.2023.28606","url":null,"abstract":"Ribonucleic acid (RNA) integrity and yield are indispensable factors for the extraction process, denoting the quality of downstream molecular analysis tools. To develop a robust method to extract RNA from Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in the presence and absence of Trigona honey, three commercial kits for RNA extraction were utilized. Total RNA was evaluated for quantity and integrity using a NanoDrop analyzer and 2100 Agilent Bioanalyzer, respectively. The 260/280 absorbance ratio of total RNA was between 1.8 and 2.1 for RNA samples while using the three commercial kits, whereas RNA integrity number (RIN) values showed low integrity of less than four. Meanwhile, when some modifications in the extraction method were applied, the integrity of the RNA samples increased, and the RIN value ranged from 7.9 to 9. We conclude that the usage of a single procedure in RNA extraction did not yield the needed quality or quantity of RNA product. Therefore, the implementation of a mixture of solid-phase extraction and liquid-liquid extraction methods has augmented the high throughput of the RNA end product. Additionally, starting with a correct cellular number through performing a viable bacterial count is considered a limiting criterion in gene expression studies through conducting Digital Reverse transcription polymerase chain reaction, microarray, or RNA sequencing.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"10 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85068509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A green chemistry approach to establish a conductometric technique for quantifying Metformin HCl in pharmaceutical samples and its greenness assessment using an analytical greenness metric calculator","authors":"S. Suryawanshi, M. Palled","doi":"10.7324/japs.2023.145086","DOIUrl":"https://doi.org/10.7324/japs.2023.145086","url":null,"abstract":"Green analytical chemistry focuses on making analytical processes safer and more environment friendly for the analyst. The primary goals of the proposed research are to develop and validate a conductometric method with green chemistry assistance for the estimation of Metformin HCl (MET), as well as to assess the method’s level of greenness using the AGREE-analytical greenness measure tool. The conductometric method for quantification of MET was developed in two steps. Step 1: Preparation of working standard solutions of MET and Step 2: Estimation of electrical conductivity of working standard solutions of MET. During the development, various dilutions of MET were prepared in Millipore water (MW) ranging from 50 to 250 µg/ml. The prepared solutions were subjected to the measurement of electrical conductivity using the conductometer ELICO CM 183EC-TDS analyzer version 2.3 instrument. The created approach was authenticated to regulate its routine capabilities in accordance with the most recent International Council for Harmonisation guidelines for regulatory recommendations. With an r 2 value of 0.999, MET demonstrated linearity concerning amount ranges from 50 to 250 µg/ml. Less than 2% RSD was observed for each set of validation parameters, which was substantially within acceptable limits. The observed recovery levels ranged from 99% to 100%. According to the AGREE metric tool calculation, the suggested method was deemed to be greener and more suitable for analyzing MET in pharmaceutical samples. The proposed research found to show advantages over reported methods in terms of usage of solvents, cost, and time of analysis. The method suggests the usage of MW as a cheap solvent, only simple dilutions and measuring the conductance of the analyte solution makes the method less time-consuming.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83776176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moringa oleifera leaf extract ameliorates early stages of diabetic nephropathy in streptozotocin-induced diabetic rats","authors":"Ruttiya Thongrung, Patchareewan Pannangpetch, L. Senggunprai, Sakkarn Sangkhamanon, Kampeebhorn Boonloh, P. Tangsucharit","doi":"10.7324/japs.2023.89429","DOIUrl":"https://doi.org/10.7324/japs.2023.89429","url":null,"abstract":"Diabetic nephropathy (DN) is a long-term complication of diabetes mellitus. The characteristic of early-stage DN is glomerular hyperfiltration that has been linked to renal fibrosis. In this study, we investigated the effect of Moringa oleifera leaf extract (MOE) on DN in streptozotocin (STZ)-induced DN rats. Rats were injected with 50 mg/kg STZ to establish the DN model. Four weeks after receiving an injection of STZ, DN rats were administered distilled water, MOE (100 or 200 mg/kg body weight/day), dapagliflozin (1 mg/kg body weight/day), or combinations of these for further 8 weeks. DN rats exhibited significantly increased blood glucose (504.00 ± 28.41 mg/dl), proteinuria (192.85 ± 41.23 mg/24 hour), albuminuria (6.68 ± 1.54 mg/24 hours), blood urea nitrogen (47.14 ± 5.18 mg/dl), and creatinine clearance (5.64 ± 0.35 ml/minute) ( p < 0.05) together with significantly increased malondialdehyde and decreased superoxide dismutase and catalase ( p < 0.05). Administration of MOE could significantly reduce the high blood glucose, impaired renal function, and oxidative stress parameters of DN rats ( p < 0.05). Histological examination of kidneys showed a thickening of the glomerular basement membrane and an increase in the mesangial matrix; all of these pathological changes were improved by MOE administration. The mRNA expression of transforming growth factor-beta 1 (TGF-β1) and collagen type IV were significantly increased in the kidney tissue of DN rats but were significantly downregulated in MOE -treated rats ( p < 0.05). MOE could alleviate DN plausibly due to its activities in reducing blood glucose, oxidative stress, and fibrosis formation by downregulating the expression of TGF-β1 and collagen type IV genes. MOE may be useful as an alternative or supplementary medicine for treatment of DN.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82240739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro study: Catechins as depigmenting agents inhibit melanogenesis on B16F0 cells","authors":"W. Widyastuti, D. Putra, S. Yenny, A. Elliyanti","doi":"10.7324/japs.2023.107125","DOIUrl":"https://doi.org/10.7324/japs.2023.107125","url":null,"abstract":"Catechins are flavonoid compounds that are the main components in gambir plants and are reported to have antioxidant activity. Research on the depigmenting agent effect of gambir catechins has not been done much. Melanogenesis inhibition effect of a compound can be done by looking at the decrease in tyrosinase (TYR) enzyme activity and melanin levels. This study aimed to test in vitro the melanogenesis inhibitory activity of catechins isolated from gambir in cell culture, including testing of cell viability, TYR activity, and decreased levels of melanin in B16F0 cells. The results showed that gambir catechins affect the viability of B16F0 cells without causing toxicity to cells. Increasing the concentration and duration of the test significantly decreased TYR activity and melanin levels in B16F0 cells ( p < 0.05). Based on the research conducted, it can be concluded that in vitro gambir catechins have an effect as a depigmenting agent.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81571584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}