journal of applied pharmaceutical science最新文献

{"title":"Subchronic toxicity test of Strychnos ligustrina extract and dihydroartemisinin-piperaquine phosphate in male and female mice","authors":"Siti Sadiah, W. Syafii, R. Sari, Y. Sani, H. Alfarisi, Haula Fadya Andini, Roro Iyaka Nuraliyu, U. Cahyaningsih","doi":"10.7324/japs.2023.36531","DOIUrl":"https://doi.org/10.7324/japs.2023.36531","url":null,"abstract":"Strychnos ligustrina extract and dihydroartemisinin-piperaquine phosphate (SL+DHP) may increase antimalarial potential. However, no research reported the toxic effect of SL+DHP. Therefore, this study investigated the subchronic toxicity of SL+DHP in male and female mice for 28 days. Subchronic toxicity tests were using National Agency of Drug and Food Control test guidelines. Mortality, bodyweight, and relative organ weight were measured. Blood samples were analyzed for hematological and biochemical parameters. Organs were examined for histopathological analysis. The highest mortality in mice was because of high doses (800 + 333 mg/kg BW) in male and female mice. The high dose significantly decreased body weight for 28-day treatments but increased after stopping administration for 2 weeks. The relative organ weight showed a significant change in the kidney, brain, and gonad of treatment groups, but its change was a recovery in satellite groups. Strychnos ligustrina extract +DHP in the high dose significantly changed the hematological and biochemical parameters of treatment groups, but these changes recovered in male and female mice of satellite groups. Histopathological examination revealed that S. ligustrina extract +DHP had a strong toxic effect in the kidney and caused ulcer compared to other organs. Subchronic toxicity of SL+DHP for 28 days was safe in low doses (200 + 111 mg/kg BW). Medium doses (400 + 222 mg/kg BW) and high doses (800 + 333 mg/kg BW) showed a toxic effect but recovered after stopping administration for 2 weeks.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82624517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The medicinal potential of plants from the Adenostemma genus","authors":"N. Nurlela, Badrunanto Badrunanto, A. Ilmiawati, W. Nurcholis, H. Takemori, Irmanida Batubara","doi":"10.7324/japs.2023.95766","DOIUrl":"https://doi.org/10.7324/japs.2023.95766","url":null,"abstract":"Adenostemma is a genus in the Asteraceae family consisting of more than 20 species distributed in tropical regions of Asia, Africa, Australia, America, and the Pacific Islands. They have been used in folk medicine as remedies for diseases such as fever, inflammation, edema, digestive disorders, and lung injury. This review aims to provide information about Adenostemma species, the traditional uses of these species, chemical constituents, and therapeutic potential based on scientific evidence. The literature search was conducted through Google Scholar, PubMed, ScienceDirect, and KNApSAcK using several search terms. It was revealed in our studies that Adenostemma plants have various secondary metabolites, including alkaloids, terpenoids, phenolics, flavonoids, fatty acids, saponins, and tannins. Antioxidant, anti-inflammatory, antimelanogenic, antitumor, and antiviral activities have been shown by scientific evidence of its extracts and isolated compounds. Adenostemma species were demonstrated by this investigation to offer a prospective source for the development of new drugs. However, phytochemical and pharmacological data are currently limited and require further studies.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89648216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-care interventions among the Jordanians during the COVID-19 lockdown: A cross-sectional study of community pharmacists’ observations","authors":"Isra Dmour, K. Al-Hamaideh, Safa’a Ali Al- Qudah, Jumana D. Al-Shawabkeh","doi":"10.7324/japs.2023.143803","DOIUrl":"https://doi.org/10.7324/japs.2023.143803","url":null,"abstract":"The COVID-19 pandemic demanded that many governments implement a comprehensive lockdown to control the spread of the disease. Several people sought self-care intervention practices to cope with this threat due to the lack of approved treatments or vaccines in the first year of the pandemic. Because hospitals and clinics were closed, community pharmacists were the first-line healthcare providers and reliable information sources. This study explores self-care measures among Jordanians during the repeated COVID-19 lockdowns. This study was conducted employing a self-administered online survey distributed to community pharmacies and medical representatives in various provinces of Jordan during the intermittent lockdown periods of the pandemic. According to the responses received from 138 participants, the elderly, chronic disease patients, and parents of young children were the major groups interested in purchasing self-care measures. Self-care measures included gloves, masks, alcohol-based disinfectants, and dietary supplements, with limited queries regarding antivirals and antimalarial drugs. The most reported sales regarding dietary supplements were vitamins B, C, and D, zinc, multivitamins, and many inquiries regarding herbs, mostly anise, lemon, garlic, ginger honey, and turmeric. In general, a decrease in sales was observed by most of the responding participants. Moreover, participants adhere to the continuous information published by the official health authorities. As frontline healthcare practitioners, community pharmacies need appropriate and mandatory training and continuous education to continue providing vital services in future pandemics.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89673471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diuretics misuse: A cross-sectional study to explore community pharmacist’s perceptions of diuretics use and misuse","authors":"K. Al-Hamaideh, Isra Dmour","doi":"10.7324/japs.2023.104335","DOIUrl":"https://doi.org/10.7324/japs.2023.104335","url":null,"abstract":"This study aims to explore the perceptions and observations of community pharmacists regarding diuretic use and misuse in Jordan and their understanding of their expected role in patient education about proper use and misuse. A cross-sectional study was conducted using an online self-administered survey distributed to community pharmacies in various locations in Jordan. Descriptive and correlation analyses are used in data evaluation. A total of 112 respondents (female 61.05% and male 38.95%) completed the survey. Community pharmacists (56.76%) confirmed that individuals without health problems misuse diuretics and 81.82% confirmed that patients are not interested in seeking medical advice before buying a diuretic. Congestive heart failure followed by ascites edema was the main reason for dispensing diuretics with and without a prescription in community pharmacies. Moreover, elderly patients mostly misuse diuretics, and patients with chronic diseases misuse prescribed diuretics, besides using them for weight and fluid loss. Furosemide was the safest diuretic and the most misused diuretic due to its low cost and availability as a locally manufactured drug within the market. For all diuretics, fatigue and electrolyte disturbances were the most reported adverse events. A low rate of dispensing spironolactone in poly cystic ovary syndrome management was reported. Since 82.88% of the participants acknowledged that diuretics are prescription-only drugs, and 87.27% confirmed that diuretics are dispensed without a prescription, most community pharmacists (97.1%) stressed the importance of educating patients about diuretics and their side effects, as well as the necessity for patients to consult their physicians. In conclusion, diuretic misuse was observed among patients in Jordan, and community pharmacists are responsible for increasing public awareness about diuretic misuse. Still, enforcing a prescription-only policy of diuretic dispensing is crucial in restricting diuretic misuse.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86828398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development, characterization and optimization of solid lipid nanoparticles of alpha-mangostin by central composite design approach","authors":"Vutti Nagendra Babu, G. S. N. K. Rao, Roja Rani Budha, R. R. Alavala, P. K. Desu, G. K. Babu, Arja Durga Prasad","doi":"10.7324/japs.2023.24237","DOIUrl":"https://doi.org/10.7324/japs.2023.24237","url":null,"abstract":"The study aims to formulate solid lipid nanoparticles (SLNPs) of the poorly bioavailable drug α-mangostin by central composite design and evaluate their in-vitro drug characteristics. The contribution of ingredients to the physicochemical characteristics of formulated SLNPs was investigated and further utilized to optimize the final formulation. For the formulation of SLNPs, hot melt homogenization method was used followed by a ultrasonication approach. The solid lipids used in the formulation include stearic acid and Precirol ATO5; the surfactant was Poloxamer 407, and the co-surfactant was sodium taurocholate to provide the negative charge. The optimized formulation’s mean particle size, entrapment efficiency, zeta potential, and drug loading were 173.6 nm, 72.42%, −43.3 mV, and 20.46%, respectively. X-ray diffractometry confirms the amorphous nature of SLNPs. Scanning electron microscopy analysis showed spherical morphology and a particle size range between 145 and 218 nm. Differential scanning calorimetry and Fourier transform infrared studies confirmed the absence of drug-excipient interactions. According to the findings, the optimum surfactant and lipid combination produced high-quality SLNPs with stable release properties for at least 6 months at room and refrigerator temperatures. The obtained results encourage that SLNPs can be used as oral drug delivery carriers for α-Mangostin, because of their exceptional properties.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79161811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC fingerprinting/GC-MS analysis, and efficacy of Hypericum perforatum against cisplatin-induced hepato-renal toxicity in mice with insights into the TXNIP/NLRP3 pathway","authors":"Maha Badr Salem, Eman Adallah Morsi, E. El-wakil, Naglaa Mohamed El- Lakkany, Tarek Abou- shousha, Heba Abdel- Hady","doi":"10.7324/japs.2023.6683","DOIUrl":"https://doi.org/10.7324/japs.2023.6683","url":null,"abstract":"Hypericum perforatum (HP) has antioxidant and anti-inflammatory characteristics. Nevertheless, its anti-inflammatory effects against hepato-renal injury have not been investigated. Therefore, this survey is designed to evaluate the prophylactic effect of HP extract against cisplatin-(CDDP-) induced hepato-renal toxicity in mice. Also, the active ingredients of HP were recognized. Hepato-renal toxicity was prompted by a single dose of CDDP (13 mg/kg, intraperitoneally). HP was tested at 50, 100, and 200 mg/kg/day for 10 days. Liver and kidney enzymes, oxidative stress, and inflammatory markers were examined, and histopathological examinations were performed. Total phenolic and flavonoid contents, besides 2,2-diphenyl-l-picrylhydrazyl radical scavenging activity, were assessed by identifying HP chemical compounds through high-performance liquid chromatography (HPLC) fingerprinting and gas chromatography-mass spectrometry. HP at 200 mg/kg improved hepatic and renal biochemical markers, reduced oxidative stress, and restored normal liver and kidney histology. Moreover, HP reduced inflammation by inhibiting TXNIP and NLRP3 tissue contents and cleaved caspase-1, interleukin-1β, and nuclear factor kappa-β expressions. The HPLC fingerprinting revealed high concentrations of ellagic acids, cinnamic acids, quercetin, and hesperidin, which may be responsible for alleviating hepato-renal damage. HP is an effective therapeutic agent against CDDP-induced hepato-renal toxicity and could be a realistic adjuvant therapy with CDDP for cancer treatment.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76382797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral diazepam suppresses pentylenetetrazole-induced seizure-like behavior in adult zebrafish: A tool for nonclinical studies","authors":"Arlindo César Matias Pereira, Arthur Arantes da Cunha, H. Carvalho, I. Ferreira, J. C. T. Carvalho","doi":"10.7324/japs.2023.133132","DOIUrl":"https://doi.org/10.7324/japs.2023.133132","url":null,"abstract":"Epilepsy is one of the most severe and common neurologic disorders, affecting more than 50 million people worldwide. This disturbance is characterized by spontaneous and recurrent convulsions due to abnormal, excessive, and synchronous electrical triggers in the neural network. This study aimed to describe the behavior analysis of epileptic behavior in adult zebrafish induced by intraperitoneal (ip) pentylenetetrazole (PTZ) and the effects of oral (po) diazepam (DZP) on it. Animals’ behavior was recorded for 20 minutes after treatment with saline solution (vehicle), PTZ (125/250/400/500 mg/kg), or cotreatment with DZP (10 mg/kg) + PTZ (250/400 mg/kg). Then, altered behavioral manifestation was scored between 0 and 6 according to the following parameters: seizure score and cumulative frequency, seizure intensity measured through the area under the curve, clonic seizure latency, tonic seizure latency, and survival rate. The control group had scores between 0 and 3 and smaller convulsion intensity when compared to the PTZ-treated group. This latter group’s scores ranged from 0 to 6. Moreover, the convulsion intensity ranged from the median (PTZ at 125 mg/kg) to high (PTZ at 250/400/500 mg/kg). The latency to score 4 was similar in all PTZ treatments. However, latency to score 5 was higher in groups treated with PTZ at 400 and 500 mg/kg and was not detected in the group treated with PTZ at 125 mg/kg. Cotreatment with DZP + PTZ had scores between 0 and 5, less convulsion intensity, and higher latency to scores 4 and 5. Animals’ death occurred only in the group treated with PTZ at 500 mg/kg. These results show behavioral characterization of seizure scores in zebrafish treated with PTZ (ip) and DZP (po). This is a valuable tool for translational research and a more feasible model to replicate data in mammals.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"373 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76432113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between tumorigenesis, metastasis, immune evasion, and chemoresistance in osteosarcoma therapy","authors":"Y. Y. Lim, A. M. A. Zaidi, M. Haque, Azizi Miskon","doi":"10.7324/japs.2023.149907","DOIUrl":"https://doi.org/10.7324/japs.2023.149907","url":null,"abstract":"There has been no significant efficacy in treating osteosarcoma (OS) metastasis after nearly four decades of trials. This motivates us to elucidate OS therapies using their four bidirectional mutation stages. The historical developments and clinical advancements are briefly described to refresh the OS therapy status quo. However, the main issue of metastasis remains unresolved, accounting for 90% of pulmonary metastasis deaths. Thus, this metastasis problem is related to immune evasion and chemoresistance induced after long-term treatment by immunotherapy for tumorigenesis. Therefore, it is rational to discuss the relationship cycles of mutation stages, including tumorigenesis, metastasis, immune evasion, and chemoresistance. Even though many combinational and targeted therapies have been developed to intensify these mutation treatments, successful clinical translations with higher cure rates are still rare. Through this review, an in-depth understanding of the bidirectional relationship between the four OS mutation stages and their respective therapies is provided. Herein, we summarise the medicines for treating tumorigenesis, including Collagen beta (1-O) galactosyl transferase 2 inhibitors, transformer 2β, and ArfGAP with GTPase domain 1, miR-148a and miR-21-5p extracellular vesicles, and the long non-coding RNA leukemia inhibitory factor receptor antisense RNA1. Following the medicines for treating metastasis are AXL receptor tyrosine kinase, miR-135a-5p, messenger RNA B-cell lymphoma-6, transforming growth factor beta 1, T-cell immunoglobulin, and mucin-domain containing protein-3, suppressor of cytokine signalling-5, cancer susceptibility 15, Krüppel-like factor 3 antisense RNA 1, programmed cell death 4, autophagy-related gene 5, and Rab22a-NeoF1. Then the medicines for treating immune evasion are N-cadherin, ubiquitin-specific peptidase 12 inhibitors, latency-associated peptide domain inhibitors, anti-Wnt2 mAb, anti-αvβ8 integrin, hexokinase-2-mediated i-kappa-b-alpha, indoleamine 2,3-dioxygenase inhibitor with NO, and TGF-βRII with anti-IgG1. Finally, the medicines for treating chemoresistance are Dihydrofolate reductase, folylpoly-γ-glutamate synthetase, heat shock protein-90AA1, XCT-790, anlotinib tyrosine kinase inhibitor, and insulin-like growth factors 1. As a result, this contribution is expected to serve as a reference and guide for scientists and clinicians.","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84844110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of effective natural compounds as HIF-1Alpha mimetics for diabetic wound healing; A systematic in-silico study","authors":"Esakkimuthukumar Mariappan, Akey Krishna Swaroop, Preeya Negi, Saranya RajanBabu, Dhinesh Kumar Murugesan, Nadimpalli Prudhvi Varma, Jubie Selvaraj","doi":"10.7324/japs.2023.135162","DOIUrl":"https://doi.org/10.7324/japs.2023.135162","url":null,"abstract":"Preliminary hypothetical assessment and in silico molecular docking of statin to VEGFR2 and VEGFR3 protein complex associated with angiogenesis and lymphangiogenesis in diabetic woundsDivya Pamu, Selvaraj Ayyamperumal, Munikumar Manne, Vyshnavi Tallapaneni, Chandrasekar Joghee Nanjan Moola, Veera Venkata Satyanarayana Reddy Karri","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"248 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135550540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive compounds from marigold processing waste: Extraction, isolation, and antidiabetic activity","authors":"Nellippatta Deepika, Basavan Duraiswamy, Salma Khanam, Divanji Maohar, Hebbani Nagarajappa, Tripathy Amrutanand","doi":"10.7324/japs.2023.126892","DOIUrl":"https://doi.org/10.7324/japs.2023.126892","url":null,"abstract":"Antioxidant Potential and Type II Diabetes Related Enzyme Inhibition Properties of Raw and Processed Legumes in Indian HimalayasDibyendu Talukdar","PeriodicalId":15126,"journal":{"name":"journal of applied pharmaceutical science","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135550723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}