In vitro antidiabetic and advanced glycation end products inhibitory activity of methanol extracts of various Dendrobium species

Q2 Pharmacology, Toxicology and Pharmaceutics

journal of applied pharmaceutical science Pub Date : 2023-01-01 DOI:10.7324/japs.2023.75102

Thaniwan Cheun-Arom, B. Sritularak

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引用次数: 0

Abstract

Dendrobium orchids are one of the most varied species. In traditional Chinese medicine, Dendrobium has been reported to have an antidiabetic effect and has been used in the preparation of herbal medicines for a long time. This study aimed to investigate the in vitro antidiabetic (α-amylase and α-glucosidase assay) activities and advanced glycation end products (AGEs) inhibitory activity of the methanol extracts of 20 Dendrobium species. Phytochemical constituents and total phenolic and total flavonoid contents of the extracts were evaluated, as well. The total phenolic content was exhibited to be in the range of 31.73–132.70 mg GAE/g, and total flavonoid content was found in the range of 58.80–237.57 mg QE/g. The highest inhibitory activity of α-amylase was reported in D. parishii Rchb.f. (IC 50 46.57 µg/ml. The most effective inhibitory activity of α-glucosidase was found in D. ellipsophyllum Tang & Wang. (IC 50 128.69 µg/ml) and D. brymerianum Rchb.f. (IC 50 138.27 µg/ml). Dendrobium brymerianum Rchb.f. and D. scabrilingue Lindl. were shown to have the most potent inhibited AGEs formation with IC 50 values of 34.44 and 46.95 µg/ml, respectively, compared to aminoguanidine (IC 50 49.81 µg/ml). As a result, several Dendrobium species displayed strong antidiabetic and AGEs inhibitory activity, and they have the potential for further development.

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不同种类石斛甲醇提取物的体外抗糖尿病和晚期糖基化终产物抑制活性

石斛兰是种类最多的兰花之一。在中医中，石斛被报道具有抗糖尿病作用，并长期用于草药的制备。本研究旨在研究20种石斛甲醇提取物的体外抗糖尿病(α-淀粉酶和α-葡萄糖苷酶测定)活性和晚期糖基化终产物(AGEs)抑制活性。测定了提取物的植物化学成分、总酚和总黄酮含量。总酚含量为31.73 ~ 132.70 mg QE/g，总黄酮含量为58.80 ~ 237.57 mg QE/g。结果表明，对α-淀粉酶的抑制活性最高。(IC 50 46.57µg/ml。对α-葡萄糖苷酶的抑制作用最明显的是汤和王。(IC 50 128.69µg/ml)和D. brymeriium Rchb.f。(IC 50 138.27µg/ml)。石斛和D. scabringue Lindl。抑制AGEs形成的ic50值分别为34.44和46.95µg/ml，而氨基胍的ic50值为49.81µg/ml。结果表明，几种石斛具有较强的抗糖尿病和抑制AGEs活性，具有进一步开发的潜力。

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来源期刊

journal of applied pharmaceutical science Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

2.20

自引率

0.00%

发文量

224

期刊介绍： Journal of Applied Pharmaceutical Science (JAPS) is a monthly, international, open access, journal dedicated to various disciplines of pharmaceutical and allied sciences. JAPS publishes manuscripts (Original research and review articles Mini-reviews, Short communication) on original work, either experimental or theoretical in the following areas; Pharmaceutics & Biopharmaceutics Novel & Targeted Drug Delivery Nanotechnology & Nanomedicine Pharmaceutical Chemistry Pharmacognosy & Ethnobotany Phytochemistry Pharmacology & Toxicology Pharmaceutical Biotechnology & Microbiology Pharmacy practice & Hospital Pharmacy Pharmacogenomics Pharmacovigilance Natural Product Research Drug Regulatory Affairs Case Study & Full clinical trials Biomaterials & Bioactive polymers Analytical Chemistry Physical Pharmacy.