Journal of Asia-pacific Entomology最新文献

{"title":"Rapid DNA release from arthropod specimens using the STEP buffer for molecular diagnostics","authors":"Junhyeong Choi ,&nbsp;Wonyong Kwun ,&nbsp;Do Eun Lee ,&nbsp;Ju Hyeon Kim","doi":"10.1016/j.aspen.2025.102493","DOIUrl":"10.1016/j.aspen.2025.102493","url":null,"abstract":"<div><div>Rapid and reliable release of genomic DNA (gDNA) from arthropod specimens is essential for molecular diagnostics, species identification, and ecological monitoring, particularly when only minute samples or limited body parts are available. Here, we present the STEP buffer, a newly formulated DNA release solution, and compare its performance with that of the previously reported DAPE buffer and water, using whole bodies of <em>Dermatophagoides pteronyssinus</em> and <em>Cimex lectularius</em> first-instar nymph, exuviae of <em>C. lectularius</em>, and hind legs of <em>Aedes albopictus</em>. Specimens were incubated in each buffer at 95°C for 5 min. The supernatant was subsequently used directly as the gDNA template for quantitative PCR, as well as for downstream compatibility testing by PCR and two types of loop-mediated isothermal amplification (LAMP) assays. The STEP buffer yielded gDNA amounts comparable to or greater than those obtained using DAPE. In addition, it facilitated complete submersion of hydrophobic arthropod cuticles during incubation. PCR assays confirmed high amplification success rates for STEP, particularly from specimens containing limited gDNA. In conventional LAMP, all buffer types supported amplification. In contrast, in colorimetric LAMP, only STEP maintained consistent detection, while DAPE caused false negatives due to pH interference. Thus, the STEP buffer provides a rapid, robust, and broadly compatible method for DNA release, enabling reliable PCR and LAMP detection even from minute arthropod samples, which is advantageous for on-site diagnostics and applications requiring specimen preservation.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102493"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145462477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wing morphological alteration in an invasive fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) following the initial period of invasion in Thailand","authors":"Chitsanuphong Phanthian ,&nbsp;Nontivich Tandavanitj ,&nbsp;Chatchawan Chaisuekul","doi":"10.1016/j.aspen.2025.102506","DOIUrl":"10.1016/j.aspen.2025.102506","url":null,"abstract":"<div><div>Wing morphology plays a key role in insect dispersal and adaptability, particularly in invasive pests like <em>Spodoptera frugiperda</em> (J. E. Smith, 1797) (Lepidoptera: Noctuidae). Environmental heterogeneity and anthropogenic pressures may shape morphometric variation across space and time. This study examined wing morphology in <em>S. frugiperda</em> collected from Thailand during 2018–2021. A total of 141 field-collected specimens from 18 cornfields and 52 laboratory-reared specimens (26 corn leaf-fed, 26 artificial diet-fed) were analyzed using fifteen landmark-based geometric morphometrics and simplified (univariate) morphometrics from three wing landmarks. Sexual dimorphism in wing morphology was evident in wild populations, but absent in laboratory-reared individuals, suggesting context-dependent expression. Significant temporal and spatial variation in wing shape, especially in females, was detected, even as overall wing size remained stable. Shape changes occurred independently of size, indicating sensitivity to environmental conditions. Laboratory-reared individuals diverged from wild specimens, with artificial diet-fed moths exhibiting the most pronounced shape differences. Corn-fed individuals were more closely resemble wild populations, highlighting diet-specific effects. Simplified morphometric traits can be used to detect broad differences, such as sexual size dimorphism and some diet effects, but fail to capture finer shape variation revealed using geometric analysis. These findings underscore the value of multivariate geometric approaches in detecting subtle morphological responses to environmental factors. This study provides morphometric baseline for evaluating phenotypic plasticity, population differentiation, and environmental influences in <em>S. frugiperda</em>. While simplified methods offer practical utility for rapid assessments, geometric morphometrics remain essential for high-resolution analysis in surveillance, ecological research, and pest management under changing environments.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102506"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the efficacy of a novel strain of Beauveria brongniartii to manage Sitobion miscanthi (Takahashi) and Rhopalosiphum padi (Linnaeus) (Hemiptera: Aphididae) in China","authors":"Xujun Tian ,&nbsp;Saige Zhu ,&nbsp;Xiao Ran ,&nbsp;Xinan Li ,&nbsp;Xiangrui Li ,&nbsp;Yunhui Zhang ,&nbsp;Rongyu Li ,&nbsp;Xun Zhu","doi":"10.1016/j.aspen.2025.102484","DOIUrl":"10.1016/j.aspen.2025.102484","url":null,"abstract":"<div><div>Wheat aphids, including <em>Sitobion miscanthi</em> (Takahashi) and <em>Rhopalosiphum padi</em> (Linnaeus), inflict serious damage to wheat crops. Consequently, it is necessary to find a new management option to reduce the damage caused by aphids. Entomopathogenic fungi serve as a tool to control the aphid population build-up. The current study explores a new entomopathogenic fungus that, infests <em>S. miscanthi</em> in China. Using morphological and molecular methods, the isolated colonies were identified as <em>Beauveria brongniartii</em> Sacc. (Petc) (Ascomycota: Cordycypitaceae)<em>.</em> Bioassay studies with purified conidial suspensions further confirmed the infectivity to the adult wheat aphids. The results showed that after 6 days of treatment with a conidia concentration of 1.0 × 10⁷ spores/mL, the mortality rates of <em>S. miscanti</em> and <em>R. padi</em> were 97.78 % and 91.11 %, respectively. However, 52.22 % of <em>S. miscanthi</em> and 47.78 % of <em>R. padi</em> adults survived after 7 days of exposure to a conidia concentration of 1.0 × 10³ spores/mL of <em>B. brongniartii.</em> Additionally, the semi-lethal time decreased with the increase of conidia concentration of the strain. To further understand and enhance the value of <em>B. brongniartii</em>, we performed whole-genome sequencing and an analysis of its strain. The genome length was 2,295,663,102 bp and 316 contigs, with an average length of 7,264,756.71 bp. Moreover, database alignment identified 455 CAZyme, 1643 TCDB, and 2850 PHI genes. The current study also explored five surface proteins of <em>B. brongniartii</em> Y618 that worked best against wheat aphids. To our knowledge, this is the first report of the pathogenicity of <em>B. brongniartii</em> against wheat aphids, and it could serve as a potential candidate for an integrated pest management plan.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102484"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altitudinal shifts and climatic sensitivity of three Ephemera species (Ephemeroptera: Ephemeridae) based on long-term freshwater monitoring in South Korea","authors":"Ye Ji Seo ,&nbsp;Sung Hwan Park ,&nbsp;Dong Gun Kim ,&nbsp;Yeon Jae Bae","doi":"10.1016/j.aspen.2025.102490","DOIUrl":"10.1016/j.aspen.2025.102490","url":null,"abstract":"<div><div>Freshwater insects are sensitive indicators of climate change, yet long-term, multi-species analyses remain limited. This study investigated altitudinal distribution shifts of three Korean mayflies (<em>Ephemera separigata</em>, <em>E. strigata</em>, <em>E. orientalis</em>) using 27 years of nationwide biomonitoring (1997–2023) across 4741 georeferenced sites. Average temperature (AT), total precipitation (P), and precipitation variability (PV) were assigned to occurrence records by region and year. Multiple linear regression (MLR) was applied to assess linear relationships between climatic variables and altitude, while random forest (RF) models captured nonlinear effects and evaluated variable importance. All three species exhibited significant downslope shifts, with <em>E. separigata</em> showing the greatest decline (−173.9 m). MLR models had low explanatory power (R² = 0.037–0.150), identifying PV as significant for <em>E. separigata</em> and <em>E. orientalis</em> (p &lt; 0.05). RF models provided improved explanatory power (R² = 0.297–0.412) and revealed that precipitation-related variables (P or PV) were generally the most influential, though the leading predictor differed among species. Notably, Phase 5 coincided with a marked increase in PV, aligning with the most pronounced downslope shifts. These results suggest that PV destabilizes headwater habitats and promotes persistence in lower, hydrologically stable reaches. Our findings highlight the importance of hydrological variability, alongside temperature, in predicting climate-driven range shifts and refining bioindicator-based conservation strategies for freshwater ecosystems.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102490"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145462473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of trap color, monochamol and α-pinene release rates, and α-pinene enantiomers on the capture of Monochamus alternatus Hope (Coleoptera: Cerambycidae)","authors":"Min-Jung Huh ,&nbsp;Il-Kwon Park","doi":"10.1016/j.aspen.2025.102481","DOIUrl":"10.1016/j.aspen.2025.102481","url":null,"abstract":"<div><div><em>Monochamus alternatus</em> Hope (Coleoptera: Cerambycidae) is a known vector of the pine wood nematode (PWN), the causal agent of pine wilt disease. Effective control of this vector is essential to prevent the spread of PWN. Although pheromone traps have been employed to monitor the seasonal occurrence of <em>M</em>. <em>alternatus</em>, they have not yet been adopted as a practical method for field-level pest control. Therefore, to utilize pheromone traps for reducing vector populations, their trapping efficiency must be enhanced beyond the levels currently used for monitoring purposes. In this study, various pheromone trap conditions were evaluated through field trials to enhance the attraction of <em>M. alternatus</em> by modifying trap color, <em>α</em>-pinene enantiomers, and the release rates of pheromone and <em>α</em>-pinene. Among six tested colors, <em>M. alternatus</em> was most strongly attracted to black traps. In the enantiomer preference test for <em>α</em>-pinene, <em>M. alternatus</em> showed no significant difference in response among the different enantiomers. To assess the effects of pheromone and <em>α</em>-pinene release rates on <em>M. alternatus</em> capture, traps were baited with lures emitting varying release rates of monochamol (7–35 mg/day) and (−)-<em>α</em>-pinene (13–65 mg/day). Traps using the release rates of monochamol (35 mg/day) and (−)-<em>α</em>-pinene (52 mg/day) that resulted in the highest captures were 59.8 % more effective than those using the lowest release rates (monochamol: 7 mg/day; (−)-<em>α</em>-pinene: 13 mg/day). Notably, while there was no significant difference in male captures, traps with the optimal release rates captured 60.5 % more females. Although this study was conducted with limited replicates and relatively short trap spacing, the findings provide valuable insight into optimizing trap performance and lay the groundwork for developing more effective control strategies against <em>M</em>. <em>alternatus</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102481"},"PeriodicalIF":1.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsions of Leucaena leucocephala wood vinegar and main constituents exhibit high mosquitocidal activity against larvae of Culex pipiens L. (Diptera: Culicidae) via oxidative stress and neurotoxicity","authors":"Nehad E.M. Taktak ,&nbsp;Noura A. Hassan ,&nbsp;Hesham M. Aly ,&nbsp;Mohamed E.I. Badawy","doi":"10.1016/j.aspen.2025.102471","DOIUrl":"10.1016/j.aspen.2025.102471","url":null,"abstract":"<div><div>The present study investigates the mosquitocidal efficacy and biochemical effect of <em>Leucaena leucocephala</em> wood vinegar, its major chemical constituents (2-methoxy-<em>p</em>-creosol and <em>o</em>-guaiacol), and their nanoemulsion formulations against <em>Culex pipiens</em> larvae. The larvicidal bioassay revealed that the products exhibited time- and concentration-dependent toxicity against larvae, with nanoemulsion formulations significantly enhanced efficacy, especially for 2-methoxy-<em>p</em>-cresol, which achieved an exceptionally low LC₅₀ of 0.09 mg/L after 48 h of exposure. Biochemical assays confirmed substantial alterations in larval enzyme activity, indicating oxidative stress and neurotoxic effects induced by the treatments. Gas chromatography–mass spectrometry (GC–MS) analysis identified key bioactive compounds, including 2-methoxy-<em>p</em>-creosol (18.48 % peak area) and <em>o</em>-guaiacol (17.32 % peak area), which were further evaluated through molecular docking against target enzymes acetylcholinesterase (AChE), adenosine triphosphatase (ATPase), glutathione S-transferase (GST), and carboxylesterase (CarE). Docking results demonstrated strong binding affinities, suggesting potential mechanisms of action. These findings highlight the potential of <em>L. leucocephala</em> wood vinegar and its nanoformulations as eco-friendly, plant-based mosquito control agents with promising bioactivity at both physiological and molecular levels.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102471"},"PeriodicalIF":1.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of solubilization tags to improve protein secretion in a baculovirus expression system","authors":"Yin Zhang ,&nbsp;Hiroaki Mon ,&nbsp;Jae Man Lee ,&nbsp;Jian Xu ,&nbsp;Kyle Dominic Barnuevo ,&nbsp;Tapas Chakraborty ,&nbsp;Kohei Ohta ,&nbsp;Michiya Matsuyama ,&nbsp;Takahiro Kusakabe","doi":"10.1016/j.aspen.2025.102456","DOIUrl":"10.1016/j.aspen.2025.102456","url":null,"abstract":"<div><div>Although the silkworm-baculovirus expression system (BES) is known as an excellent recombinant secreted protein expression system, some recombinant proteins are difficult to mass produce. One reason for this is that the protein structure itself is unstable and protein aggregation occurs. In this study, we used the chub mackerel leptin-A (cmLepA), a protein that has been shown to induce gonadotropin secretion in fish, as a model protein and examined the effects of eleven fusion peptides that are expected to enhance the solubility of recombinant proteins. Our results showed that soluble secretion levels of the recombinant LepA (rLepA) were significantly increased in the serum of baculovirus-infected silkworms when BmThymosin, ScSUMO, BmSUMO, GB1, or T7SET solubility tags were fused. The purified rLepA proteins were directly assayed in fish cells for the biological activity without cleavage of the fusion tags, revealing that the BmThymosin-fused protein exhibited comparable activity to that of tag-free LepA. Collectively, our findings provide a useful set of fusion tags designed to improve the secretion and solubility of proteins of interest when using the silkworm-baculovirus expression system.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102456"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first cixiid planthopper (Fulgoromorpha: Cixiidae) from Lower Cretaceous Jinju Formation (Republic of Korea)","authors":"Mathieu Boderau ,&nbsp;Gi-Soo Nam ,&nbsp;Corentin Jouault ,&nbsp;André Nel","doi":"10.1016/j.aspen.2025.102472","DOIUrl":"10.1016/j.aspen.2025.102472","url":null,"abstract":"<div><div>A new extinct genus and species of Cixiidae are described as <em>Jinjucixius fui</em> gen. et sp. nov. from the Lower Cretaceous of Jinju Formation (Republic of Korea). This new taxon is one of the earliest representatives of Cixiidae and extends the fossil record of the family to this outcrop, being the first Fulgoromorpha described from this formation. The description of this new taxon allowed us to discuss the phylogenetic relationships between cixiid lineages.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102472"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the feasibility of ethyl formate fumigation as a stand-alone phytosanitary treatment of Bactrocera dorsalis (Diptera: Tephritidae) in mandarin fruit","authors":"Tae‐Hyung Kwon ,&nbsp;Dongbin Kim ,&nbsp;Kyeongnam Kim ,&nbsp;Gwang-Hyun Roh ,&nbsp;Bong-Su Kim ,&nbsp;Sung-Eun Lee ,&nbsp;Jia-Wei Tay ,&nbsp;Byung-Ho Lee ,&nbsp;Dong H. Cha","doi":"10.1016/j.aspen.2025.102466","DOIUrl":"10.1016/j.aspen.2025.102466","url":null,"abstract":"<div><div>Oriental fruit fly, <em>Bactrocera dorsalis</em>, is one of the most destructive quarantine pests of fruits and vegetables, with increasing incursions reported worldwide. During a <em>B. dorsalis</em> outbreak, all fruits and potential host materials within the quarantine area are destroyed to prevent further spread. As a first step toward developing an emergency treatment to allow fruit movement from a <em>B. dorsalis</em> quarantine area, we evaluated the feasibility of using ethyl formate (EF) fumigation as a stand-alone phytosanitary treatment against <em>B. dorsalis</em>. In EF efficacy trials with <em>B. dorsalis</em> eggs and larvae in the absence of fruit (naked conditions), eggs were the most tolerant life stage, with LCt₉₉ values—lethal concentration × time products that cause 99 % mortality—of 716.9 and 1,271.0 g h/m³ at 20 and 4 °C, respectively, followed by first and third instar larvae. When <em>B. dorsalis</em> were artificially inoculated into mandarin fruit, significantly greater EF doses were necessary to control <em>B. dorsalis</em> inside fruit, with the LCt₉₉ values for eggs increased to 1,606.0 and 1,761.0 at 20 and 4 °C, respectively. Although these calculated EF doses were effective against <em>B. dorsalis</em>, they caused significant damage to quality of mandarins, including reduced sugar content and increased fruit decay. These findings indicate that EF fumigation alone is not a feasible stand-alone treatment for disinfesting <em>B. dorsalis</em> in mandarins. We discuss future research directions aimed at maintaining treatment efficacy while minimizing fruit damage by combining EF fumigation with other phytosanitary treatments.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102466"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ribavirin showed insect growth regulator activity to Helicoverpa armigera","authors":"Li Xu ,&nbsp;Yin Huang ,&nbsp;Zezheng Liu ,&nbsp;Qi Zuo ,&nbsp;Minghui Long ,&nbsp;Yichen Fu ,&nbsp;Runqiang Liu ,&nbsp;Yunchao Kan ,&nbsp;Dongzhi Li","doi":"10.1016/j.aspen.2025.102457","DOIUrl":"10.1016/j.aspen.2025.102457","url":null,"abstract":"<div><div>Ribavirin is a nucleoside analog used to control virus disease clinically. In this study, toxicity determination, transcriptome analysis and ELISA were employed to explore its toxicity and potential mechanism on <em>Helicoverpa armigera</em> larvae. Results suggested that ribavirin showed stomach toxicity to <em>H. armigera</em>, but low contact toxicity. Chemicals 1,2,4-triazole and 1,2,4-triazole-3-carboxamide showed low stomach toxicity to <em>H. armigera</em>, indicating 1,2,4-triazole and furanose were both the key chemical structure to ensure the larvicidal activity of ribavirin. Transcriptome and qRT-PCR results validated that ribavirin treatment inhibited the expression of genes in chitin biosynthesis, lipid biosynthesis and cuticle protein genes. Ribavirin treatment also decreased the Juvenile Hormone (JH) titer, but showed no influence to ecdysone titer. These results indicated that ribavirin treatment inhibited JH biosynthesis and interfered new cuticle formation, which resulted to the failure of <em>H. armigera</em> larvae molting and normal pupation. These results provided a novel lead compound for IGR development to control <em>H. armigera</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102457"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}