Journal of Asia-pacific Entomology最新文献_第9页

Identification and phylogenetic of cysteine peptidase genes from the bamboo pest Hippotiscus dorsalis, based on RNA-seq and PacBio Iso-Seq 基于 RNA-seq 和 PacBio Iso-Seq 的竹类害虫 Hippotiscus dorsalis 半胱氨酸肽酶基因的鉴定和系统发育

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-10-24 DOI: 10.1016/j.aspen.2024.102328

Mingzhen Zhao , Lulu Dai , Shaoyong Zhang

{"title":"Identification and phylogenetic of cysteine peptidase genes from the bamboo pest Hippotiscus dorsalis, based on RNA-seq and PacBio Iso-Seq","authors":"Mingzhen Zhao , Lulu Dai , Shaoyong Zhang","doi":"10.1016/j.aspen.2024.102328","DOIUrl":"10.1016/j.aspen.2024.102328","url":null,"abstract":"<div><div>One of the insects that cause the most damage to Moso bamboos in the southern regions of China is <em>Hippotiscus dorsalis</em> (Hemiptera: Pentatomidae). However, the study of molecular and growth development analysis is limited because its genetic resources are still in the blank stage. In order to improve our knowledge of the molecular traits of this insect pest, we first generated the transcriptome from <em>H dorsalis</em> using PacBio Iso-Seq. With an average length of 1495 bp, 207,663 nonredundant full-length reads were produced. Using five databases, we annotated 36,238 transcripts: Uniprot (24,388), GO (1160), NR (24,110), Pfam (9333), and KEGG (14,497). According to aligning of each transcript in the NR database, most of them (85.147 %) were annotated into the functional genes from <em>Halyomorpha halys</em>. In the meantime, we discovered 33,689 long noncoding RNAs (lncRNAs), 4408 transcription factors (TFs), and 586 alternative splicing (AS) events, which involved in transcriptional regulation. Additionally, the candidate cathepsin genes from the bamboo pest were examined in order to comprehend how they adapted to their host. To sum up, our findings offer a fresh resource for comprehensive transcriptional data and insights into the genetics and gene expression of <em>H dorsalis</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102328"},"PeriodicalIF":1.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carvacrol acts on the larval midgut of Spodoptera frugiperda by destroying the tissue structure and altering the bacterial community 香芹酚通过破坏组织结构和改变细菌群落对蚜虫幼虫中肠产生作用

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-10-24 DOI: 10.1016/j.aspen.2024.102329

Jingjing Zhang , Cuiting Liu , Xinyi Xie , Xuanyue Yu , Yuting Huang , Rui Tang , Jian Zeng , Benshui Shu

{"title":"Carvacrol acts on the larval midgut of Spodoptera frugiperda by destroying the tissue structure and altering the bacterial community","authors":"Jingjing Zhang , Cuiting Liu , Xinyi Xie , Xuanyue Yu , Yuting Huang , Rui Tang , Jian Zeng , Benshui Shu","doi":"10.1016/j.aspen.2024.102329","DOIUrl":"10.1016/j.aspen.2024.102329","url":null,"abstract":"<div><div>The fall armyworm, Spodoptera frugiperda, has become a global invasive pest in recent years, threatening agricultural production. Carvacrol (CAR) is a multifunctional plant-derived compound and exhibited significant bioactivities against many pests. Our previous study found that CAR inhibited growth and development in <em>S. frugiperda</em>. However, the effects of CAR on the midgut of this pest remain unknown. In this study, the actions of CAR on the larval midgut of <em>S. frugiperda</em> were investigated. The results found that 1.0 and 2.0 g/kg CAR treatments destroyed the structure of the larval midgut based on hematoxylin-eosin staining and transmission electron microscope observation. In addition, 2.0 g/kg CAR exposure significantly altered the larval midgut bacterial community composition and structure. The results of the linear discriminant analysis effect size (LEfSe) analysis suggest that six bacterial genera contributed to the different structures of the larval bacterial community. Furthermore, PICRUSt2 analysis found that 16 bacterial function categories were altered by CAR treatment, of which 8 were significantly increased. Our results provided new insight into the toxicological mechanisms of CAR against S. frugiperda larvae and laid the foundation for the field application of S. frugiperda control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102329"},"PeriodicalIF":1.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on the mosquitocidal property of cis-13-Octadecenoic acid isolated from Andrographis paniculata against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) 从穿心莲中分离的顺式-13-十八碳烯酸对埃及伊蚊和库蚊（双翅目：蚤科）的杀蚊特性研究

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-10-24 DOI: 10.1016/j.aspen.2024.102330

Jelin Vilvest , M.C. John Milton , Alex Yagoo , Kedike Balakrishna

{"title":"Investigation on the mosquitocidal property of cis-13-Octadecenoic acid isolated from Andrographis paniculata against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)","authors":"Jelin Vilvest , M.C. John Milton , Alex Yagoo , Kedike Balakrishna","doi":"10.1016/j.aspen.2024.102330","DOIUrl":"10.1016/j.aspen.2024.102330","url":null,"abstract":"<div><div>Mosquitoes are a critical global public health concern, causing millions of deaths each year due to the viral infections and diseases they spread. In recent decades, synthetic insecticides and repellents have been used to control mosquito populations and virus transmission, but they often harm the environment and non-target organisms, including humans. This research investigates the isolation, characterization, and structural elucidation of <em>cis</em>-13-Octadecenoic acid derived from Andrographis paniculata, and its effectiveness against the immature stages of Aedes aegypti and Culex quinquefasciatus. The extracts from A. paniculata underwent fractionation, with the most bioactive fractions being further purified and analyzed. Spectroscopic techniques were employed to confirm the structure of <em>cis</em>-13-Octadecenoic acid. Biological assays were then performed to evaluate its ovicidal, larvicidal, and pupicidal activities. <em>cis</em>-13-Octadecenoic acid exhibited notable ovicidal activity, causing 25 % mortality in Ae. aegypti eggs and 29 % mortality in Cx. quinquefasciatus eggs at a concentration of 2 ppm. Additionally, it showed strong larvicidal and pupicidal activities, with LC<sub>50</sub> values of 2.67 ppm and 3.39 ppm for Ae. aegypti larvae and pupae, and 3.10 ppm and 2.93 ppm for Cx. quinquefasciatus larvae and pupae, respectively. These findings demonstrate the potential of <em>cis</em>-13-Octadecenoic acid as an effective natural compound for mosquito control, highlighting the broader importance of natural products as alternatives to synthetic insecticides in the management of vector-borne diseases.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102330"},"PeriodicalIF":1.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hibernation changes tissue specific lipophilic profile of Chilo partellus (Swinhoe) 冬眠改变了奇洛鱼（Swinhoe）的组织亲脂性特征

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-10-22 DOI: 10.1016/j.aspen.2024.102327

Ashok K. Sau , Mukesh K. Dhillon , Sandeep Kumar , Aditya K. Tanwar

{"title":"Hibernation changes tissue specific lipophilic profile of Chilo partellus (Swinhoe)","authors":"Ashok K. Sau , Mukesh K. Dhillon , Sandeep Kumar , Aditya K. Tanwar","doi":"10.1016/j.aspen.2024.102327","DOIUrl":"10.1016/j.aspen.2024.102327","url":null,"abstract":"<div><div><em>Chilo partellus</em> undergo hibernation to survive under harsh winter conditions. We investigated changes in lipophilic profiles of larval and pupal whole body and tissues (haemolymph, fat bodies and integument) of hibernation and non-hibernation <em>C. partellus</em> strains. Significantly higher amount of lipids was found in whole body of pre-hibernation and hibernation larvae than non-hibernation larvae. At tissue level, total lipids were significantly higher in haemolymph and fat bodies of pre-hibernation, and integument of non-hibernation larvae. In whole body of larvae and pupae of hibernation and non-hibernation strains, most abundant fatty acids were oleic acid (16.4%–23%), palmitic acid (14.6%–19.3%), linoleic acid (9.3%–11.6%), palmitoleic acid (3.7%–7.3%) and stearic acid (1.7%–2.8%). Further, at tissue level, myristic, palmitoleic, palmitic, linoleic and oleic acids in fat bodies; and stearic acid in the integument of hibernation larvae were higher as compared to other body parts and larval stages of <em>C. partellus</em>. In pupal tissues, myristic, palmitic and stearic acids were higher in the haemolymph and fat bodies of non-hibernation pupae than post-hibernation pupae. Among the unsaturated fatty acids (UFAs), the palmitoleic acid significantly increased in all the larval tissues, and linoleic acid in fat bodies and integument, while oleic acid significantly decreased in integument of hibernation as compared to non-hibernation larvae of <em>C. partellus</em>. These information on adaptive remodelling of lipids at tissue level might help in future physiological, molecular, ecological and pest management studies on <em>C. partellus.</em></div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102327"},"PeriodicalIF":1.1,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Negative relationships between elevated developmental temperatures and morphological traits of different castes of bumblebees (Bombus terrestris) 发育温度升高与不同种群熊蜂形态特征之间的负相关关系

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-10-13 DOI: 10.1016/j.aspen.2024.102326

Han Soo Kim , Jin Myeong Kim , Weiyue Qiu , Hyung Joo Yoon , Kyeong Yong Lee , Kwang Sik Lee , Byung Rae Jin

{"title":"Negative relationships between elevated developmental temperatures and morphological traits of different castes of bumblebees (Bombus terrestris)","authors":"Han Soo Kim , Jin Myeong Kim , Weiyue Qiu , Hyung Joo Yoon , Kyeong Yong Lee , Kwang Sik Lee , Byung Rae Jin","doi":"10.1016/j.aspen.2024.102326","DOIUrl":"10.1016/j.aspen.2024.102326","url":null,"abstract":"<div><div>Climate change can negatively impact the morphology and behavior of bumblebees. In particular, it can decrease the body size of workers, thereby reducing foraging activity. However, the morphological impacts of higher developmental temperatures on different castes of bumblebees remain insufficiently understood. In this study, we investigated the effects of elevated developmental temperatures on the morphological traits of different castes of bumblebees (<em>Bombus terrestris</em>). We reared queens that emerged from hibernation at different developmental temperatures: optimal (constant temperature of 27 °C), moderately high (constant temperature of 32 °C), and high (35 °C; by transferring the colony from 32 °C to 35 °C after the emergence of 10 workers). The body weight, head capsule width, body width, body length, and wing length of different castes of bumblebees significantly differed at elevated developmental temperatures. The highest impact was noted on the body width and body weight of workers, body width and wing length of queens, and body length, head capsule width, and wing length of males. Among the different castes of bumblebees, workers were most impacted by elevated developmental temperatures, followed by queens and then males. In contrast, the relative ratio of wing length to body length suggested the allometry of wing length in bumblebee castes at elevated developmental temperatures. These findings indicate that elevated developmental temperatures resulting from climate change may negatively affect the morphological traits of different castes of bumblebees.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102326"},"PeriodicalIF":1.1,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissemination of phosphorescent microbial agents by honey bees (Apis mellifera L.) in apple orchards 蜜蜂（Apis mellifera L.）在苹果园中传播磷光微生物制剂

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-10-09 DOI: 10.1016/j.aspen.2024.102325

Jin Hyeok Choi , Soon-Il Kim , Woohyung Lee , Yong Hwan Lee , Kil Won Kim , Myeong-Lyeol Lee , Hyung Wook Kwon

{"title":"Dissemination of phosphorescent microbial agents by honey bees (Apis mellifera L.) in apple orchards","authors":"Jin Hyeok Choi , Soon-Il Kim , Woohyung Lee , Yong Hwan Lee , Kil Won Kim , Myeong-Lyeol Lee , Hyung Wook Kwon","doi":"10.1016/j.aspen.2024.102325","DOIUrl":"10.1016/j.aspen.2024.102325","url":null,"abstract":"<div><div>Honey bees are commonly used for pollination in apple orchards. This study aimed to develop a bee-vectoring scheme whereby honey bees (<em>Apis mellifera</em> L.) deliver microbial agents (<em>Bacillus amyloliquefaciens</em> MBI600) against fire blight from a dispenser at the entrance of the beehive to apple flowers. The efficiency of disseminating phosphorescent microbial agents (PMA) by honey bees was compared across four apple orchards; (A) with installed insect nets, (B) isolated on an island, (C) adjacent to other apple orchards and apiaries, and (D) near C within 50 m and without beehives. The average detection ratio of PMA on bees visiting flowers was 100 % in orchard (A) and (B), whereas in (C) and (D) it was 54.5 % and 40.0 %, respectively. Detection of PMA on apple blossoms was also 100 % in (A) and (B), but significantly low in (C) and (D) at 52.8 % and 12.9 %. In orchards (A) and (B), PMA adhered to pistils at high ratios of 98.0 % and 96.3 %, while in orchard (C), it was significantly lower at 9.3 %. Phosphorescence intensity in flowers and the proportion of apple pollen loads collected from incoming bees were also investigated. Finally compared to the orchard (A) and (B), open orchard (C) and (D) showed significantly lower microbial agent spread efficiency by honey bee vectoring. For open orchards, supplemental strategy to enhance bee vectoring could include increasing the number of hives in the orchard and temporarily relocating neighbor’s beehives during flowering, or supplying additional dispensers to nearby apiaries.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102325"},"PeriodicalIF":1.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mode of action underlying toxicity of Camptothecin to Spodoptera frugiperda (Lepidoptera) larvae 喜树碱对 Spodoptera frugiperda（鳞翅目）幼虫的毒性所依据的作用模式

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-09-25 DOI: 10.1016/j.aspen.2024.102322

Jun-li Lv , Wen-qing Lai , Lei Pang , Xiao-ying Zhang , Kai-yi Zheng , Qiang Zhang , Zhan-peng Lu , Zhi-sheng Wang , Fasihul Lisan , Xue-yang Wang , Lu-feng Hu , Ming-jie Deng

{"title":"Mode of action underlying toxicity of Camptothecin to Spodoptera frugiperda (Lepidoptera) larvae","authors":"Jun-li Lv , Wen-qing Lai , Lei Pang , Xiao-ying Zhang , Kai-yi Zheng , Qiang Zhang , Zhan-peng Lu , Zhi-sheng Wang , Fasihul Lisan , Xue-yang Wang , Lu-feng Hu , Ming-jie Deng","doi":"10.1016/j.aspen.2024.102322","DOIUrl":"10.1016/j.aspen.2024.102322","url":null,"abstract":"<div><div>Camptothecin (CPT) is derived from <em>Camptotheca acuminate</em> and its main action is the inhibition of the DNA topoisomerase I (Top I) complex. Therefore, CPT and its derivatives are always used as antitumor and antimicrobial agents and also have some insecticidal effects. However, the mode of action underlying the toxicity of CPT to the virulence of pests is still not fully understood. In this study, the effects of CPT on <em>Spodoptera frugiperda</em> (<em>S. frugiperda</em>) were determined by performing toxicity assessments, metabolomics, and mechanism analysis. The results showed that CPT increased the efficiency of inhibition of larval weight in a dose and time-dependent manner. In addition, the metabolomics of 5th-instar larval hemolymph was determined by UPLC-MS/MS and <sup>1</sup>H NMR analysis. UPLC-MS/MS analysis showed that the energy metabolic pathway was more important than the amino acid pathway for the resistance of <em>S. frugiperda</em> to CPT. Furthermore, metabolites involved in the energy metabolic pathway were analyzed using <sup>1</sup>H NMR, and fumarate and pyruvate were identified as interesting metabolites. Bioassays confirmed that they play a key role in enhancing the resistance of <em>S. frugiperda</em> larvae to CPT. Finally, the underlying mechanisms were found to be related to the restorative effects of the two metabolites on metabolic pathways associated with the TCA cycle. In a word, CPT is a potential biological insecticide that exhibits a strong toxicological effect against <em>S. frugiperda</em> by inhibiting the TCA cycle, which provides theoretical support for applying CPT in the control of pests.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102322"},"PeriodicalIF":1.1,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Megachilid bees (Hymenoptera: Anthophila: Megachilidae) of Ladakh: A morphological and molecular approach 拉达克的巨蜂（膜翅目：蝠科：Megachilidae）：形态学和分子方法

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-09-24 DOI: 10.1016/j.aspen.2024.102324

Spalzin Angmo , Sayan Sardar , Tsering Stobdan , Archana Chauhan , S.I. Kazmi , A. Rameshkumar

{"title":"Megachilid bees (Hymenoptera: Anthophila: Megachilidae) of Ladakh: A morphological and molecular approach","authors":"Spalzin Angmo , Sayan Sardar , Tsering Stobdan , Archana Chauhan , S.I. Kazmi , A. Rameshkumar","doi":"10.1016/j.aspen.2024.102324","DOIUrl":"10.1016/j.aspen.2024.102324","url":null,"abstract":"<div><div>The megachilid bees of Ladakh are documented based on the collections deposited in the National Zoological Collection and the collections made from Leh district by S. Angmo during 2020–2023. Accounts of sixteen species known from this region belonging to three tribes viz., Anthidiini (03 species), Megachilini (09 species) and Osmiini (01 species) are provided. Thirteen species have been detected among which seven species, <em>Stelis scutellaris</em> Morawitz, <em>Coelioxys conoideus</em> (Illiger), <em>Coelioxys rufescens</em> Lepeletier and Serville, <em>Megachile creutzburgi</em> (Tkalců), <em>Megachile leachella</em> Curtis, <em>Megachile lagopoda</em> (Linnaeus) and <em>Megachile maritima</em> (Kirby) are new to India, while three among the remaining six species are new to Ladakh. Floral associations for the recently collected specimens are provided. The Mitochondrial COI sequences of 09 species are deposited in BOLD/NCBI among which sequences for 03 species are novel deposits. Based on the COI sequences already available in the database and the sequences deposited, a phylogenetic tree for the studied specimens has been included.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102324"},"PeriodicalIF":1.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the enzymatic response of Plutella xylostella L. (Lepidoptera: Plutellidae) to Metarhizium anisopliae (Metschnikoff) Sorokin (Ascomycota: Hypocreales) Infection: A Comprehensive study 调查鳞翅目褐飞虱 Plutella xylostella L. (Lepidoptera: Plutellidae) 对 Metarhizium anisopliae (Metschnikoff) Sorokin (Ascomycota: Hypocreales) 感染的酶反应：综合研究

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-09-24 DOI: 10.1016/j.aspen.2024.102321

Abarna V.P. , Muthuswami M. , Jeyarajan Nelson S , Johnson Thangaraj Edward Y.S. , Manikanda Boopathi N. , Anita B. , Jeya Sundara Sharmila D.

{"title":"Investigating the enzymatic response of Plutella xylostella L. (Lepidoptera: Plutellidae) to Metarhizium anisopliae (Metschnikoff) Sorokin (Ascomycota: Hypocreales) Infection: A Comprehensive study","authors":"Abarna V.P. , Muthuswami M. , Jeyarajan Nelson S , Johnson Thangaraj Edward Y.S. , Manikanda Boopathi N. , Anita B. , Jeya Sundara Sharmila D.","doi":"10.1016/j.aspen.2024.102321","DOIUrl":"10.1016/j.aspen.2024.102321","url":null,"abstract":"<div><div>The diamondback moth (DBM), <em>Plutella xylostella</em>, poses a significant threat to cruciferous crops due to its resistance to conventional insecticides. Entomopathogenic fungi (EPF), such as <em>Metarhizium anisopliae</em> offer a promising solution for the management of <em>P. xylostella</em>. When exposed to lethal or sub-lethal doses of microbial insecticides especially EPF, insects often mount various immune responses as a survival mechanism, including the activation of detoxification and protective enzymes. In this study, <em>M. anisopliae</em> (Ma1) isolated from soil resulted in 94.00 ± 0.245 percent mortality of DBM at a conidial load of 1 x 10<sup>9</sup> conidia/ml, with an LC<sub>50</sub> value of 2.70 x 10<sup>6</sup> conidia/ml. The activity of various detoxifying and free radical scavenging enzymes was assessed in whole-body samples of <em>P. xylostella</em> at varying time intervals after inoculation with the entomopathogenic fungi. The results showed that the activity of the enzymes increased after 48 h of inoculation, reaching a maximum at 96 h after inoculation. After 96 h, the activity of these enzymes started to decline significantly. In conclusion, the <em>M. anisopliae</em> isolate Ma1 was found to be highly effective against <em>P. xylostella.</em> Therefore, it could be formulated using appropriate carrier material and used as a component in Integrated pest management programs. Elevated levels of detoxification and free radical scavenging enzyme expression helps in understanding the immune mechanisms in <em>P. xylostella</em> in response to <em>M. anisopliae</em> infection.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102321"},"PeriodicalIF":1.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-description of the genus Talanga Moore and the description of a new endemic species from Indonesia (Lepidoptera: Crambidae; Spilomelinae) 重新描述 Talanga Moore 属并描述印度尼西亚特有的一个新种（鳞翅目：蝶科；鞘翅目）

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2024-09-24 DOI: 10.1016/j.aspen.2024.102323

Darmawan Darmawan , Jackson F. Watung , Awit Suwito , Raden Pramesa Narakusumo , Encilia Encilia , Agmal Qodri , Dhian Dwibadra , Djunijanti Peggie , Rosichon Ubaidillah , Hari Sutrisno

{"title":"Re-description of the genus Talanga Moore and the description of a new endemic species from Indonesia (Lepidoptera: Crambidae; Spilomelinae)","authors":"Darmawan Darmawan , Jackson F. Watung , Awit Suwito , Raden Pramesa Narakusumo , Encilia Encilia , Agmal Qodri , Dhian Dwibadra , Djunijanti Peggie , Rosichon Ubaidillah , Hari Sutrisno","doi":"10.1016/j.aspen.2024.102323","DOIUrl":"10.1016/j.aspen.2024.102323","url":null,"abstract":"<div><div>Six species of the genus <em>Talanga</em> Moore 1855 are re-described based on both morphological and genitalia characters. <em>T. tolumnialis</em> Walker is not included due to the lack of available of material. Morphological characters such as a tapering, oblique outwardly antemedial band on the forewing and two black metallic spots externally bordered by similar minute interciliar spots on the hindwing, are proposed as the most appropriate characters to define the genus <em>Talanga</em>. The study also reveals that certain genitalia characters, such as a spatula-shaped uncus, a simple valva with a medial rod-like plate, a long cylindrical phallus without cornuti, a bundle of pencil hairs at the coremata base in male genitalia, and a pair of ovate or circular signa medially bearing denticles in female genitalia, are also diagnostic of this genus. The description of a new endemic species from Foja Mountain, <em>Talanga horakae</em> <strong>sp. nov.</strong>, is presented, as well as an identification key to Indonesian species, and images of adults and genitalia of the studied species.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"27 4","pages":"Article 102323"},"PeriodicalIF":1.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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