Journal of Asia-pacific Entomology最新文献

{"title":"Chemical, antioxidant, anti-inflammatory, antibacterial, and cytotoxicity assessments of Apis mellifera intermissa venom collected in Northeast Algeria","authors":"Faouzi Dahdouh ,&nbsp;Gizem Kursunluoglu ,&nbsp;Amira Bourouba ,&nbsp;Amina Bourouba ,&nbsp;Mukerrem Betul Yerer ,&nbsp;Houria Djebar-Berrebbah","doi":"10.1016/j.aspen.2025.102449","DOIUrl":"10.1016/j.aspen.2025.102449","url":null,"abstract":"<div><div>This study characterized the venom of the Algerian honeybee, Apis mellifera intermissa, and evaluated its key biological activities. Biochemical analysis by High-performance liquid chromatography (HPLC) analysis revealed that melittin, the main component of bee venom, constituted approximately 38 % of its dry weight. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed the presence of major components, including melittin (∼3–5 kDa), phospholipase A₂ (∼15–20 kDa), and hyaluronidase (∼44 kDa). The venom exhibited strong antioxidant activity, as demonstrated by Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Potent, broad-spectrum antibacterial activity was observed against <em>Pseudomonas</em> aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Enterococcus faecalis, and Acinetobacter baumannii, yielding low minimum inhibitory and bactericidal concentrations (MIC/MBC). The anti-inflammatory potential was evidenced by the inhibition of bovine serum albumin (BSA) denaturation. Furthermore, the venom exhibited significant cytotoxicity against MCF-7 and MDA-MB-231 human breast cancer cell lines, with half-maximal inhibitory concentrations (IC₅₀) of 17.17 µg/mL and 11.90 µg/mL, respectively. These findings highlight venom as a rich source of bioactive compounds with multifaceted therapeutic potential for pharmacological development.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102449"},"PeriodicalIF":1.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual and combined efficacy of zeolite and spinetoram for controlling Rhyzopertha dominica (F.) (Bostrichidae: Coleoptera); impact of temperature, relative humidity and grain types","authors":"Rameesha Amjad Ali ,&nbsp;Mansoor-ul-Hasan ,&nbsp;Eman Fayad ,&nbsp;Seham Sater Alhelaify ,&nbsp;Saqer S. Alotaibi ,&nbsp;Anam Moosa ,&nbsp;Faisal Zulfiqar","doi":"10.1016/j.aspen.2025.102448","DOIUrl":"10.1016/j.aspen.2025.102448","url":null,"abstract":"<div><div>Laboratory experiments were conducted to investigate the insecticidal potential of zeolite (ZeoFeed) and spinetoram alone and in combination against the adults of lesser grain borer, <em>Rhyzopertha dominica</em> (F.) (Bostrichidae: Coleoptera). Bioassays were carried out at 15, 25, and 35 °C and 55 and 75 % relative humidity (R.H.) on wheat, rice, and maize. Efficacy of zeolite was determined at 2500, 500, and 700 ppm in first bioassay, followed by efficacy of spinetoram at 0.25, 0.50 and 1 ppm in second bioassay, and combined effectiveness of zeolite and spinetoram at 750 and 1 ppm doses in third bioassay. Adult mortality in all bioassays was recorded 1-, 3-, 7- and 14-days post-exposure. Results showed that mortality was positively related with dose and exposure interval. More insects died with increasing temperature and decreasing R.H. Wheat was more susceptible followed by rice and maize. Mortality was more in combined treatments (750 ppm + 1 ppm) with complete control of insects at 35 °C + 55 % R.H., 14 days of post-exposure in all tested commodities. This is the first report on the use of zeolite combined with insecticide to combat <em>R. dominica</em> under these conditions. The study suggests that zeolite in combination with spinetoram can effectively protect grains from this species, considering factors such as temperature and relative humidity.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102448"},"PeriodicalIF":1.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional response and instar preference of Encarsia formosa (Hymenoptera: Aphelinidae) parasitizing Bemisia tabaci (Hemiptera: Aleyrodidae) at different densities and temperatures","authors":"Bo Zhang ,&nbsp;Guo-Ting Yuan ,&nbsp;Peng Han ,&nbsp;Krutmuang Patcharin ,&nbsp;Perumal Vivekanandhan ,&nbsp;Jin-Long Zhang ,&nbsp;Jiao Kong ,&nbsp;Guo-Hua Chen ,&nbsp;Xiao-Ming Zhang","doi":"10.1016/j.aspen.2025.102445","DOIUrl":"10.1016/j.aspen.2025.102445","url":null,"abstract":"<div><div><em>Bemisia tabaci</em> (Gennadius) is among the most serious threats to global agricultural production. Biological control of <em>B. tabaci</em> using the parasitoid <em>Encarsia formosa</em> Gahan is effective on various crops. However,the biological control of <em>B. tabaci</em> could be affected by various abiotic factors. Understanding the effects of temperature on parasitoid efficacy is one of the key ascpects in this case. In this study, the functional response, searching efficiency, and parasitism preference of <em>E. formosa</em> on early and late nymphal instars of <em>B. tabaci</em> at different temperatures were investigated. We found that the functional response conformed to the type II model at 19, 22, 25, 28 and 31 °C. The parasitoid efficiency of <em>E. formosa</em> toward nymphal <em>B. tabaci</em> was the highest at 28 °C. Searching efficiency of <em>E. formosa</em> decreased with the increase in host density. The Hassell-Varley interference model simulation indicated intraspecific interference in <em>E. formosa</em>. <em>E. formosa</em> preferred to parasitize late instar nymphs of <em>B. tabaci</em> compared to the early instar nymphs. In conclusion, <em>E. formosa</em> releases at 25–31 °C with a parasitoid-to-host ratio of 1:50 is considered optimal to control <em>B. tabaci</em> in field, which minimizes costs and maximizes parasitism rate.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102445"},"PeriodicalIF":1.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Varroa destructor infestation and botanical oils on physiological characteristics and mechanism of defensive behavior of Carniolan and Italian Apis mellifera","authors":"Amany E. El-Nagar ,&nbsp;Zeinab A. Mohamed ,&nbsp;Wafa A.H. Alkherb ,&nbsp;Dina F. Alhashdi ,&nbsp;Abd El-Aziz M. Mohsen","doi":"10.1016/j.aspen.2025.102435","DOIUrl":"10.1016/j.aspen.2025.102435","url":null,"abstract":"<div><div>The purpose of this study was to assess the physiological and behavioral reactions of Italian and Carniolan honey bees (<em>Apis mellifera</em>) to botanical treatments and infestations of <em>Varroa destructor</em>. Measurements of amino acid profiles, enzyme activities, nutrient contents, grooming, and hygienic practices were used to evaluate the effects of infestation and treatments (formic acid, apricot seed extract, garlic oil, and camphor oil). In both bee subspecies, <em>Varroa</em> mite infestation markedly changed the levels of amino acids, especially lowering proline, alanine, and methionine. Essential amino acid levels were higher in uninfested bees, whereas methionine and glutamic acid levels were higher in bees treated with formic acid. In general, the lowest concentrations of amino acids were found in apricot oil. The untreated controls had the highest levels of total carbohydrates, proteins, and lipids, while the bees treated with garlic oil or formic acid had the lowest levels. While invertase activity peaked in bees treated with apricot oil, the enzymatic activities of GOT, GPT, amylase, and esterases decreased when exposed to formic acid and garlic oil. The enzyme levels of Italian and Carniolan bees varied, with the latter typically exhibiting greater activity. Grooming responses changed over five days after treatment, according to behavioral assays. Apricot extract and garlic oil significantly influenced grooming behavior in colonies, with the highest levels of hygienic behavior observed within the first 24 h. These findings suggest <em>Varroa</em> infestation impairs bee physiology, but certain botanical treatments especially apricot and garlic oils may enhance bee resilience through improved biochemical balance and behavioral defenses.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102435"},"PeriodicalIF":1.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo and in silico evaluation of the toxicological and biochemical effects of Cd 4-formylbenzoate pyrazine complex on Galleria mellonella L. (Lepidoptera: Pyralidae)","authors":"Ahmet Harmankaya ,&nbsp;Serkan Sugeçti ,&nbsp;Mustafa Sertçelik ,&nbsp;Cihat Çelik ,&nbsp;Ender Büyükgüzel ,&nbsp;Hacali Necefoğlu ,&nbsp;Kemal Büyükgüzel","doi":"10.1016/j.aspen.2025.102443","DOIUrl":"10.1016/j.aspen.2025.102443","url":null,"abstract":"<div><div>Environmental pollution resulting from the use of insecticides in conventional pest control necessitates the exploration of novel chemical compounds with reduced toxicity. In this study, the Cd 4-formylbenzoate pyrazine complex was initially investigated in silico to assess its interaction with the juvenile hormone of <em>Galleria mellonella</em>. The binding affinity of the complex to the juvenile hormone was determined to be −8.1 kcal/mol. Based on the strong binding affinity observed in the in silico analysis, the biochemical effects of the complex on <em>G. mellonella</em> hemolymph were further evaluated in vivo. The results demonstrated a significant increase in the levels of alanine transaminase, aspartate transaminase, and gamma-glutamyl transferase, which are key enzymes involved in amino acid metabolism in the exposed samples. Furthermore, cellular damage was observed in the hemolymph following exposure to the complex. Additionally, crucial metabolic enzymes, including lactate dehydrogenase, creatine kinase, alkaline phosphatase, and amylase, were adversely affected. Collectively, these findings indicate that the Cd 4-formylbenzoate pyrazine complex exerts toxic effects on the model pest <em>G. mellonella</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102443"},"PeriodicalIF":1.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hygiene conditions explain larval density-dependent performance in Plutella xylostella with sufficient food","authors":"Aiying Lu ,&nbsp;Leyun Wang","doi":"10.1016/j.aspen.2025.102444","DOIUrl":"10.1016/j.aspen.2025.102444","url":null,"abstract":"<div><div>Larval density is an important factor influencing insect growth, development and population dynamics. Under conditions of limited food resources, higher larval density leads to reduced food availability per individual, causing adverse outcomes. When food was abundant, how larval density influences insect performance and its underlying mechanism have been understudied. We compared fitness traits of <em>Plutella xylostella</em> (Lepidoptera: Plutellidae), over a wide range of rearing densities (1, 4, 7 and 10 larvae per Petri dish) provided with sufficient common cabbage leaves. <em>Plutella xylostella</em> reared at 10 larvae per container had significant reductions in the pupal weight, realized fecundity, and adult life span when compared with those reared at lower densities. To find out the underlying mechanism, we further tested the hypothesis that the hygiene condition in part mediates larval density-dependent performance of larvae with sufficient food. We investigated the effect of 3 hygiene conditions (clean, control, mess: indicated by feces accumulation rates) on fitness of <em>P. xylostella</em> at the same rearing density (10 larvae per Petri dish) provided with sufficient common cabbage leaves. Results confirmed the important role of hygiene conditions in mediating the responses of larva fitness to larval density that the mess condition significantly reduced the pupal weight, total survival rate, realized fecundity, adult life span, and the development rates of completing a life cycle in comparison to cleaner treatments. These findings highlight that, even with sufficient food, rearing insects in different densities and hygiene conditions can largely affect the fitness outcomes. This provides critical insights for understanding the ecology of insects feeding in high densities.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102444"},"PeriodicalIF":1.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of global climate change on insect populations, distribution, and its dynamics","authors":"Fredrick Ojija ,&nbsp;Marco Mng’ong’o ,&nbsp;Becky N. Aloo ,&nbsp;Gabriel Mayengo ,&nbsp;Mlyashimbi Helikumi","doi":"10.1016/j.aspen.2025.102442","DOIUrl":"10.1016/j.aspen.2025.102442","url":null,"abstract":"<div><div>Insects are vital to various ecosystems as pollinators, decomposers, and food sources for many organisms. They dominate diverse terrestrial (e.g.<em>,</em> glassland) and aquatic (lakes, oceans, rivers, etc.) ecosystems. Previous studies report that more than half of the estimated 2.0 million species of living organisms identified on our planet are insects. However, global climate change (GCC), characterised by rising temperatures and altered precipitation patterns, significantly impacts their populations worldwide. We reviewed the literature to provide an overview of GCC events in insects. Collectively, the study findings reveal that global temperature and precipitation change are among the extreme GCC events affecting more than 30% of insect population, distribution, physiology, feeding habits, interactions, migration, and communication across the globe. The climate change intensifies insect cycles and insect damage in agroecosystems. In response, insect species alter their geographic ranges and phenology, changing population dynamics and interactions. GCC also influences reproductive patterns, including mating behaviour and breeding synchrony. Warmer global temperatures might advance or delay insect emergence, causing mismatches with food availability or pollination partners. While some insect populations may adapt, extreme heat events or prolonged droughts exceeding their physiological tolerance result in population declines or local extinctions. Predictions suggest that up to 65% of insect populations could face extinction within the next century due to increasing climate change. Thus, understanding these impacts is essential for predicting the ecological consequences of the GCC and developing effective conservation strategies to mitigate such impacts and protect insect biodiversity and ecosystem services.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102442"},"PeriodicalIF":1.1,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lethal and sublethal effects of azadirachtin on Riptortus pedestris (F.) and its egg parasitoids, Gryon japonicum (Ashmead) and Ooencyrtus nezarae Ishii","authors":"Young-gyun Park ,&nbsp;Naresh Dangi ,&nbsp;Un Taek Lim","doi":"10.1016/j.aspen.2025.102441","DOIUrl":"10.1016/j.aspen.2025.102441","url":null,"abstract":"<div><div>Azadirachtin may cause acute toxicity and alter behavior of natural enemies. We evaluate the lethal and sublethal effect of five concentrations of azadirachtin through topical application and residual exposure on <em>Riptortus pedestris</em> (F.). Its effects were also assessed against egg parasitoids, <em>Gryon japonicum</em> (Ashmead) and <em>Ooencyrtus nezarae</em> Ishii, by pre- and post-parasitization treatment of host eggs. The toxicity of azadirachtin to <em>R. pedestris</em> increased significantly with higher concentrations. A morphological deformity in nymphs was also observed in topical application after molting into the next instar. Azadirachtin significantly reduced egg parasitism by <em>G. japonicum</em> and <em>O. nezarae</em> at concentrations of 15 and 10 mL/L. Interestingly, the male ratio of <em>G. japonicum</em> progeny was increased significantly when host eggs were treated with azadirachtin before parasitization. However, the risk levels of azadirachtin to <em>G. japonicum</em> and <em>O. nezarae</em> were classified as harmless and slightly harmful, respectively, at a concentration of 10 mL/L. Thus, this concentration could be a potential alternative to broad-spectrum chemical insecticides for managing <em>R. pedestris</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102441"},"PeriodicalIF":1.1,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of RNAi candidate target genes in Monolepta hieroglyphica (Motschulsky) for potential pest control applications","authors":"Xu Chen ,&nbsp;Ning Fu ,&nbsp;Qingxuan Xu ,&nbsp;Chun Zhang ,&nbsp;Su Wang ,&nbsp;Da Xiao","doi":"10.1016/j.aspen.2025.102440","DOIUrl":"10.1016/j.aspen.2025.102440","url":null,"abstract":"<div><div><em>Monolepta hieroglyphica</em> (Motschulsky) (Coleoptera; Chrysomelidae) is a major agricultural pest affecting maize crops in northern and northwestern China. RNA interference (RNAi) technology offers a strategy for sustainable pest control by targeting and silencing specific genes, reducing reliance on chemical pesticides. In this study, we investigated four genes from <em>M. hieroglyphica</em> genome: V-type ATPase subunit A (<em>MhVATP-A</em>), coat protein complexes I <em>β’</em> subunit (<em>MhCOPI-β’</em>), cytochrome P450 reductase (<em>MhCPR</em>) and tyrosine decarboxylase (<em>MhTDC</em>) as potential RNAi targets for RNAi-mediated pest control. Through dsRNA injections in adult, we successfully achieved gene silencing in <em>M. hieroglyphica</em>, resulting in significantly reduced expression levels of the target genes. Silencing of <em>MhVATP-A</em> and <em>MhCOPI-β’</em> resulted in a marked increase in mortality (60–80%), suggesting that these genes play critical roles in essential physiological functions, such as cellular PH regulation and vesicular transport. In contrast, <em>MhCPR</em> suppression resulted in moderate mortality (30%), indicating that while it influences detoxification processes, it may be less effective as a primary target for pest control. <em>MhTDC</em> silencing, involved in nervous system regulation, had no significant impact on adult insect survival, possibly due to compensatory physiological mechanisms. Overall, our findings demonstrate the potential of RNAi technology to target essential genes in <em>M. hieroglyphica,</em> leading to significant insect mortality. The results highlight <em>MhVATP-A</em> and <em>MhCOPI-β’</em> as promising targets for RNAi-based transgenic maize, offering a species-specific approach for sustainable pest management in maize production. Further investigation is required to assess the effectiveness of RNAi across different development stages and environmental conditions.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102440"},"PeriodicalIF":1.3,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Trypanosoma evansi and Theileria/Babesia sp. in Tabanus spp. populations (Diptera: Tabanidae) from Nueva Ecija, Philippines","authors":"Frances Paula L. Miaral ,&nbsp;Keisuke Suganuma ,&nbsp;Claro N. Mingala ,&nbsp;Jose Ma M. Angeles ,&nbsp;Derick Erl P. Sumalapao ,&nbsp;Thaddeus M. Carvajal ,&nbsp;Adrian Miki C. Macalanda ,&nbsp;Cristian C. Lucañas ,&nbsp;Maria Angenica F. Regilme ,&nbsp;Gabriel Alexis S. Tubalinal ,&nbsp;Maria Luz B. Belleza ,&nbsp;Arlene G. Bertuso","doi":"10.1016/j.aspen.2025.102439","DOIUrl":"10.1016/j.aspen.2025.102439","url":null,"abstract":"<div><div>Hematophagous flies of the family Tabanidae (Order Diptera) are important nuisance pests and vectors of several diseases in both animals and humans. In this study, the potential role of tabanids in the transmission of protozoans such as <em>Babesia</em> sp., <em>Theileria</em> sp. and <em>Trypanosoma evansi</em> was determined. Nzi traps were installed near backyard farms in Brgy. Catalanacan, Science City of Muñoz, Nueva Ecija, Philippines from May to June 2023 for the collection of tabanids. The species, relative abundance, and density of the collected tabanids were recorded. While the occurrence of <em>Tr. evansi</em> (n = 383) and <em>Theileria/Babesia</em> sp. (n = 382) in the dissected proboscis of tabanids were determined using polymerase chain reaction (PCR). The presence of human blood among the samples that were positive for <em>Tr. evansi</em> and <em>Theileria/Babesia</em> sp. was also determined as an indicator of potential human exposure to the protozoans. A total of 4,317 tabanids were collected belonging to <em>Tabanus partitus</em> (99.95 %, 4,315/4,317) and <em>Ta. reducens</em> (0.05 %, 2/4,317). Using conventional PCR, the occurrences for <em>Tr. evansi</em> and <em>Theileria/Babesia</em> sp. were 47.26 % (181/383) and 31.15 % (119/382) in <em>Ta. partitus</em>, respectively, while both were detected in 6.01 % (23/383) of the proboscis. Twelve samples that were positive for <em>Theileria/Babesia</em> sp. were subjected to sequencing and were found to belong to the genus <em>Theileria</em>. About 18.41 % (51/277) of the samples positive for either or both protozoans have detectable human blood, indicating that humans have been bitten by tabanids in the study area, thus demonstrating the risk of exposure to hemoprotozoan-carrying tabanids.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102439"},"PeriodicalIF":1.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}