Journal of Asia-pacific Entomology最新文献

Differential electrophysiological responses of wingless and winged forms of three non-host-alternating aphids to plant volatile compounds

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-07 DOI: 10.1016/j.aspen.2025.102395

Ya-Ya Li , Jinyue Qiao , Jicheng Wang , Xiujie Jia , Yuting Tang , Kairu Hou , Deguang Liu , Li Chen

{"title":"Differential electrophysiological responses of wingless and winged forms of three non-host-alternating aphids to plant volatile compounds","authors":"Ya-Ya Li , Jinyue Qiao , Jicheng Wang , Xiujie Jia , Yuting Tang , Kairu Hou , Deguang Liu , Li Chen","doi":"10.1016/j.aspen.2025.102395","DOIUrl":"10.1016/j.aspen.2025.102395","url":null,"abstract":"<div><div>From spring to autumn, aphids produce two types of asexual morphs: wingless and winged. Olfactory cues are often utilized by the winged morph in long-distance host plant selection, and by the wingless morph in short-range host plant location. Electroantennogram (EAG) responses to a range of plant volatiles were recorded from wingless and winged forms of the English grain aphid <em>Sitobion avenae</em>, greenbug <em>Schizaphis graminum</em>, and pea aphid <em>Acyrthosiphon pisum</em>. The response patterns of the three aphid species were similar, although some variations did occur. (<em>E</em>)-2-Hexenal elicited the largest EAG responses in both asexual forms (i.e., wingless and winged) of the three species. In general, green leaf volatiles and benzaldehyde produced relatively large EAGs. The winged form was more responsive than the wingless form to all the plant volatile compounds tested but 4-ethylbenzaldehyde for <em>S. graminum</em>, and hexanal and benzyl acetate for <em>A. pisum</em>. This study indicates that both forms of <em>S. avenae</em>, <em>S. graminum</em>, and <em>A. pisum</em> are able to detect various volatile compounds, and generally higher EAG response in winged forms suggest that they have a more responsive olfactory system than wingless forms, and are better-adapted for switching host plants under unfavorable environmental conditions.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102395"},"PeriodicalIF":1.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new species and new records with a key to South Korean moth flies (Diptera: Psychodidae: Psychodinae)

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-06 DOI: 10.1016/j.aspen.2025.102394

Sang-Woo Kim , Sam-Kyu Kim

引用次数: 0

Transcriptomic analysis of Drosophila melanogaster larvae exposed to dimethyl phthalate reveals molecular insights into developmental retardation

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-05 DOI: 10.1016/j.aspen.2025.102391

Dan He , Jinxu Liu , Weihui Xu , Yunlong Hu , Wenjing Chen , Zhenyu Fan , Zhigang Wang

{"title":"Transcriptomic analysis of Drosophila melanogaster larvae exposed to dimethyl phthalate reveals molecular insights into developmental retardation","authors":"Dan He , Jinxu Liu , Weihui Xu , Yunlong Hu , Wenjing Chen , Zhenyu Fan , Zhigang Wang","doi":"10.1016/j.aspen.2025.102391","DOIUrl":"10.1016/j.aspen.2025.102391","url":null,"abstract":"<div><div>Dimethyl phthalate (DMP), a ubiquitous environmental pollutant, has been recognized for its capacity to impede biological development. Therefore, it is crucial to understand the mechanism by which DMP inhibits biological processes. This study employed the <em>Drosophila melanogaster</em> larvae as a model organism to explore the mechanisms of developmental retardation induced by DMP exposure via a transcriptomic approach. The results showed that 1.2 g/L DMP exposure induced developmental retardation in larvae, as evidenced by a significant increase in the larval-to-pupal transition time compared to the standard diet. Furthermore, transcriptome sequencing revealed 4451 differentially expressed genes (DEGs) in DMP-exposed larvae, comprising 2430 up-regulated and 394 down-regulated genes. These DEGs were predominantly involved in pathways such as carbohydrate metabolism, signal transduction, transporter metabolism, endocrine system, and tumorigenesis. This indicates that developmental toxicity associated with DMP exposure is intricately linked to these pathways, underscoring the need to address the detrimental effects of DMP exposure. To summarize, exposure to DMP at a specific concentration has a deleterious effect on organismal development.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102391"},"PeriodicalIF":1.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection of tomato yellow leaf curl virus changes metabolites of the whitefly, Bemisia tabaci

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-05 DOI: 10.1016/j.aspen.2025.102393

Junbeom Lee , Dae-Weon Lee

引用次数: 0

Molecular identification and functional analysis of the putative ecdysone receptor in Spodoptera litura

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-01 DOI: 10.1016/j.aspen.2025.102390

Ziqian Tong , Huixuan Shi , Zhuo Liu , Donghai Zhang , Dandan Li , Yunchao Kan , Huili Qiao

{"title":"Molecular identification and functional analysis of the putative ecdysone receptor in Spodoptera litura","authors":"Ziqian Tong , Huixuan Shi , Zhuo Liu , Donghai Zhang , Dandan Li , Yunchao Kan , Huili Qiao","doi":"10.1016/j.aspen.2025.102390","DOIUrl":"10.1016/j.aspen.2025.102390","url":null,"abstract":"<div><div>20-hydroxyedysone (20E) is an important hormone that regulates the process of molting and metamorphosis in insects. Ecdysone receptor (EcR) is one of the most important primary nuclear receptor for 20E. To clarify the essential function of EcR during the development of <em>Spodoptera litura</em>, the gene encoding the putative ecdysone receptor isoform A (<em>SlEcRA</em>) was cloned and verified. Multiple sequence comparisons and the subsequent phylogenetic study indicated that SlEcRA protein exhibited a high degree of conservation and significant homology with EcRA proteins in other insects. Spatiotemporal expression analysis showed that <em>SlEcRs</em> were predominantly expressed during the larval molting and prepupa stages, with the highest expression in fat body. Moreover, the expression of <em>SlEcRs</em> were induced by 20E in fat body, midgut and integument. Silencing of <em>SlEcRs</em> led to increased mortality and decreased larval weight during larval development. A combination treatment of dsSlEcRs and low-concentration emamectin benzoate effectively increased larvae mortality at 48 h. Furthermore, the larval mortality of Chi-dsSlEcRs nanoparticles by epidermal infiltration was much higher than the naked dsSlEcRs by injection. Co-delivery of Chi-dsSlEcRs and emamectin benzoate exhibited shorter action time and higher mortality rate on larvae. These findings not only highlight the potential of the putative <em>SlEcR</em> as an RNA interference target for <em>S. litura</em> control, but also provide a new idea for reducing the reliance on chemical insecticides in agricultural pest control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102390"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of fecundity related traits and transcriptome in two isofemale lines of the egg parasitoid, Trichogramma dendrolimi (Matsumura) 卵寄生虫树枝金龟子（松村）两个异雌品系繁殖力相关性状和转录组的比较分析

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-01 DOI: 10.1016/j.aspen.2025.102387

Hong-Yu Liang , An-Ke Liu , Ning He , Jin-Cheng Zhou , Xiao-Hui Ma , Li-Sheng Zhang , Wu-Nan Che , Hui Dong

{"title":"Comparative analysis of fecundity related traits and transcriptome in two isofemale lines of the egg parasitoid, Trichogramma dendrolimi (Matsumura)","authors":"Hong-Yu Liang , An-Ke Liu , Ning He , Jin-Cheng Zhou , Xiao-Hui Ma , Li-Sheng Zhang , Wu-Nan Che , Hui Dong","doi":"10.1016/j.aspen.2025.102387","DOIUrl":"10.1016/j.aspen.2025.102387","url":null,"abstract":"<div><div>The egg parasitoid, <em>Trichogramma dendrolimi</em>, plays an important role as a biological control agent for the control of various lepidopteran pests. The reproductive capacity of <em>T. dendrolimi</em> is essential for both mass-rearing and effective pest control. In this study, a <em>T. dendrolimi</em> line with a high fecundity trait (Td-H) and another with a low fecundity trait (Td-L) were selected from 13 isofemale lines collected across different regions of China. The fecundity trait of Td-H and Td-L females was validated through assessments of oocyte number in the ovary, fecundity, body size, and adult longevity. To investigate the expression patterns of fecundity-related genes in <em>T. dendrolimi</em>, transcriptomes of Td-H and Td-L females were sequenced and compared. A total of 441 differentially expressed genes (DEGs), including 181 up-regulated genes and 260 down-regulated genes, were identified. Moreover, 11 genes were specifically expressed in Td-H females, while 16 genes were exclusive to Td-L females. These DEGs were mainly enriched in categories such as “metabolic process” (102 DEGs), “organelle”(53 DEGs), “catalytic activity” (90 DEGs), and “binding” (174 DEGs) in GO analysis, as well as various metabolic pathways (58 DEGs) and the “Neuroactive ligand-receptor interaction” pathway (22 DEGs) in KEGG analysis. The accuracy of seven up-regulated DEGs and seven down-regulated DEGs in the transcriptome was confirmed using RT-qPCR. These findings will be helpful for investigating the regulatory mechanisms associated with the fecundity traits of <em>T. dendrolimi</em> and provide a reference for improving biological control agents against pests.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102387"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematicidal potential of berberine and sanguinarine from Chelidonium majus against Meloidogyne incognita

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-26 DOI: 10.1016/j.aspen.2025.102384

Fangwei Lu , Zixuan Wang , Youchuang Chang , Dandan Cao , Guixia Liu

{"title":"Nematicidal potential of berberine and sanguinarine from Chelidonium majus against Meloidogyne incognita","authors":"Fangwei Lu , Zixuan Wang , Youchuang Chang , Dandan Cao , Guixia Liu","doi":"10.1016/j.aspen.2025.102384","DOIUrl":"10.1016/j.aspen.2025.102384","url":null,"abstract":"<div><div>In this study, we investigated the effects of <em>Chelidonium majus</em> and its main alkaloids on the activity of <em>Meloidogyne incognita</em>. The dipping method evaluated the effects of <em>C. majus</em> crude extracts of the above- and belowground parts(ACE and BCE) and main alkaloids at different concentrations and durations on egg hatching and the second-stage juveniles (J2s) activity. The results revealed that ACE and BCE of C. majus, berberine and sanguinarine, have inhibitory effects on J2s and egg hatching. J2s mortality and egg hatching inhibition increased with higher crude extract and alkaloid concentrations and longer treatment time. At 90 mg/mL for 48 h, ACE and BCE resulted in J2s mortalities of 89.01 % and 97.43 %, and egg hatching inhibition rates of 100.00 % and 98.67 %. The LC<sub>50</sub> of ACE is 24.42 mg/mL, and the LC<sub>50</sub> of BCE is 20.75 mg/mL. Exposed to a treatment of 2 mg/mL for 24 h, the corrected mortality rates for berberine and sanguinarine were 90.48 % and 92.08 %, with their LC<sub>50</sub> values being 0.102 and 0.089 mg/mL, demonstrating significant nematicidal activity. Berberine and sanguinarine have egg hatching inhibition rates of 94.55 % and 87.13 %. Furthermore, we determined the absolute contents of berberine and sanguinarine in the above- and belowground parts of <em>C. majus.</em> The contents of berberine and sanguinarine base in the aboveground parts were 14.65 and 79.33 µg/g, while the underground parts was 180.26 and 714.06 µg /g. Our results indicate that <em>C. majus</em> crude extract and their potent alkaloids hold potential as natural nematicides for controlling <em>M. incognita.</em></div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102384"},"PeriodicalIF":1.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical compositions and insecticidal activities of Clausena lenis (Rutaceae) and Clausena excavata (Rutaceae) essential oils against two stored-product insects

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-24 DOI: 10.1016/j.aspen.2025.102388

Yu-Chen Zhang , Ao Liu , Yuan Zhang , Yong-Qing Gu , Shu-Shan Du

{"title":"Chemical compositions and insecticidal activities of Clausena lenis (Rutaceae) and Clausena excavata (Rutaceae) essential oils against two stored-product insects","authors":"Yu-Chen Zhang , Ao Liu , Yuan Zhang , Yong-Qing Gu , Shu-Shan Du","doi":"10.1016/j.aspen.2025.102388","DOIUrl":"10.1016/j.aspen.2025.102388","url":null,"abstract":"<div><div><em>Tribolium castaneum</em> Herbst, 1797 (Coleoptera: Tenebrionidae) and <em>Lasioderma serricorne</em> Fabricius, 1792 (Coleoptera: Anobiidae) are two globally prevalent stored-product pests. Traditional management strategies primarily involve the use of synthetic insecticides and repellents. However, plant essential oils (EOs) pose lower risks to human health and ecological environment. This study focused on two <em>Clausena</em> species plants, <em>Clausena lenis</em> and <em>Clausena excavata</em>, exploring the insecticidal and repellent properties of EOs extracted from different parts of them against <em>T. castaneum</em> and <em>L. serricorne</em>. The EOs were extracted via hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC–MS). A total of 57 components were identified. The EOs from the branches and leaves of two <em>Clausena</em> plants were primarily composed of sesquiterpenes, while <em>C. lenis</em> fruits EO was mainly composed of monoterpenes (58.79 %) and esters (38.95 %). <em>Clausena lenis</em> leaves EO demonstrated the highest contact toxicity against <em>T. castaneum</em>, with a mortality rate of 72 % at a concentration of 50 %, while <em>C. lenis</em> branches EO was particularly effective against <em>L. serricorne</em>, with an LD<sub>50</sub> of 8.24 mg/L air. In addition, <em>C. lenis</em> fruits EO exhibited the best repellent effect against these two pests. According to these results, <em>C. lenis</em> and <em>C. excavata</em> show promise to be developed into eco-friendly botanical insecticides for the prevention and control of stored-product pests, especially for application in agricultural storage. These findings highlight the potential for these EOs to serve as sustainable alternatives to synthetic insecticides, offering a safer and environmentally friendly approach to pest management in stored agricultural products.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102388"},"PeriodicalIF":1.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promotional activity of an uncharacterized protein Bm0979 on Nosema bombycis in silkworm, bombyx mori 未定性蛋白质 Bm0979 对家蚕蚕蛾的促进作用

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-24 DOI: 10.1016/j.aspen.2025.102389

Yujia Fang , Qiong Yu , Wenzhe Chen , Zhenghao Han , Qingsheng Qu , Zhenwei Jia , Shidong Jiang , Jie Cheng , Xudong Tang , Ping Qian

{"title":"Promotional activity of an uncharacterized protein Bm0979 on Nosema bombycis in silkworm, bombyx mori","authors":"Yujia Fang , Qiong Yu , Wenzhe Chen , Zhenghao Han , Qingsheng Qu , Zhenwei Jia , Shidong Jiang , Jie Cheng , Xudong Tang , Ping Qian","doi":"10.1016/j.aspen.2025.102389","DOIUrl":"10.1016/j.aspen.2025.102389","url":null,"abstract":"<div><div>Microsporidia are intracellular parasites that infect a diverse range of hosts, including economically significant insects like the silkworm (<em>Bombyx mori</em>). In this study, we investigated the effects of the microsporidian pathogen <em>Nosema bombycis (Nb)</em> on <em>B. mori</em>, with a focus on the cloning and functional characterization of the gene <em>Bm0979</em>. Our findings indicate that <em>Bm0979</em> has at least two isoforms and is significantly upregulated in response to <em>Nb</em> infection. Functional assays involving overexpression and knockdown of <em>Bm0979</em> in silkworm cells revealed that this gene promotes <em>Nb</em> proliferation, identifying it as a potential target for enhancing silkworm resistance to pebrine disease. Transcriptome sequencing further revealed that Bm0979 modulates the expression of genes related to the PI3K/Akt signaling pathway, which is implicated in immune responses and cellular metabolism. These findings provide new insights into the molecular mechanisms underlying host-pathogen interactions in silkworms and could contribute to the development of strategies for controlling microsporidia infections in sericulture.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102389"},"PeriodicalIF":1.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-22 DOI: 10.1016/j.aspen.2025.102386

Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin

{"title":"The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)","authors":"Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin","doi":"10.1016/j.aspen.2025.102386","DOIUrl":"10.1016/j.aspen.2025.102386","url":null,"abstract":"<div><div>The silk of silkworm, <em>Bombyx mori</em>, consists mainly of proteins, which contain some small proteins with specialized functions that confer special properties to silk, such as antimicrobial properties. Serine protease inhibitors (SPIs) are one of them, and the types that have been identified are seroin, serpin, Kunitz type, and Kazal type, but the function of the Kunitz/Kazal type is not known. As compared to others, Kazal molecular weight is relatively minimal. In this study, we focused on the function of a Kazal-type inhibitor BmSPI2, and its sequence characterization and expression profile were first refined. To clarify its function, BmSPI2 recombinant protein was expressed in sf9 cells using baculovirus expression system. Further testing revealed that BmSPI2 could inhibit trypsin, <em>α-</em>chymotrypsin, and plasmin, whereas has only some effect on proteinase K. This suggests that BmSPI2 has significant serine protease inhibitory activity. In-depth analysis revealed that BmSPI2 also effectively inhibited fibrinogen degradation mediated by fibrinolytic enzymes. Finally, we examined its antimicrobial-related functions using prepared antibodies and found that BmSPI2 was also able to directly bind to bacteria and fungi to inhibit their proliferation. The results of the study enriched the function of BmSPI2 and laid a theoretical foundation for its development and utilization.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102386"},"PeriodicalIF":1.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0