Journal of Asia-pacific Entomology最新文献_第2页

Possible interactive risk of copper hydroxide, inorganic bactericide of fire blight with dinotefuran and triflumizole on honey bee 氢氧化铜、无机杀菌剂与呋喃、三氟唑对蜜蜂可能的交互作用风险

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-06 DOI: 10.1016/j.aspen.2025.102475

Hyeonjeong Jang , Tekalign Begna , Sukjun Sun , Delgermaa Ulziibayar , Chuleui Jung

{"title":"Possible interactive risk of copper hydroxide, inorganic bactericide of fire blight with dinotefuran and triflumizole on honey bee","authors":"Hyeonjeong Jang , Tekalign Begna , Sukjun Sun , Delgermaa Ulziibayar , Chuleui Jung","doi":"10.1016/j.aspen.2025.102475","DOIUrl":"10.1016/j.aspen.2025.102475","url":null,"abstract":"<div><div>In spring 2021, abnormal honey bee mortality was observed near apple orchards in Cheongsong, South Korea, following copper-based fire blight control. This study evaluated the acute toxicity of copper hydroxide and basic copper sulfate to honey bees, assessed residue levels in field-collected bees, and investigated potential synergistic effects with co-occurring pesticides. Five-step serial dilutions of the recommended field concentration (RFC) were used for acute oral and contact toxicity assays. ICP-OES was used to quantify copper residues in lab-exposed and field-collected bees, while multi-residue pesticide analysis was performed with LC-MS/MS and GC–MS/MS. Copper hydroxide showed the highest oral toxicity, with both LD<sub>50</sub> and HQ values indicating moderate toxicity. Field-collected bees exhibited higher copper bioaccumulation than control, comparable to lab-exposed bees with a 10<sup>−2</sup> RFC dilution, which remained well under the LC<sub>50</sub>. This implies that copper alone is not the cause of the witnessed phenomenon. Among detected pesticides, dinotefuran exhibited high HQ values, exceeding elevated risk threshold. Triflumizole and buprofezin showed minimal risk; however, triflumizole was considered likely to interact with dinotefuran, with their short half-lives potentially contributing to increased toxicity in bees. The combination of copper, dinotefuran, and triflumizole suggests potential interactive toxicity contributing to the observed mortality. These findings underscore the need to account for pesticide mixtures in practical management strategies, as even low-risk compounds can contribute to elevated toxicity when applied together. To prevent unintended harm to honey bees, a more integrated and pollinator-conscious approach to pesticide use is essential.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102475"},"PeriodicalIF":1.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of environmentally-acquired gut symbionts on fitness and behavior of adult female host, Riptortus pedestris (Hemiptera: Alydidae) 环境获得性肠道共生体对成年雌寄主步行Riptortus（半翅目：盲蝽科）适应度和行为的影响

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-04 DOI: 10.1016/j.aspen.2025.102469

Minhyung Jung, Joo-Young Kim, Doo-Hyung Lee

{"title":"Effects of environmentally-acquired gut symbionts on fitness and behavior of adult female host, Riptortus pedestris (Hemiptera: Alydidae)","authors":"Minhyung Jung, Joo-Young Kim, Doo-Hyung Lee","doi":"10.1016/j.aspen.2025.102469","DOIUrl":"10.1016/j.aspen.2025.102469","url":null,"abstract":"<div><div>Bean bug, <em>Riptortus pedestris</em> (Hemiptera: Alydidae), acquires its symbiotic bacteria belonging to family <em>Burkholderiaceae</em> from soil environments in every generation. Beneficial effects of its symbiotic bacteria have been demonstrated mainly with immature stages of <em>R. pedestris</em>, while remaining their effects on adult biology largely unexplored. In this study, we investigated effects of the gut symbiont, <em>Caballeronia insecticola</em>, on fitness and behavior traits of female <em>R. pedestris</em> adults. First, the symbiotic bacteria exerted adverse effects on survivorship, with which survival rates decreased to 86.7 % and 56.7 % in apo-symbiotic and symbiotic females, respectively, at 60 days after adult emergence. Despite a reduced longevity of symbiotic females, the fecundity of symbiotic insects was on average 2.0-fold greater than that of apo-symbiotic ones during this period. We also evaluated overwintering survivorship in laboratory conditions and found that symbiotic females exhibited significantly higher survivorship than that of apo-symbiotic ones. Second, behavioral assays demonstrated that symbiotic <em>R. pedestris</em> displayed enhanced feeding efficiency by consuming higher amounts of soybean per feeding attempt. For both walking and flight capacity, symbiosis yielded significant increases in mobility than apo-symbiotic ones. In particular, symbiotic females flew on average 1.3 km over 4 h, which was 138.1-fold greater than apo-symbiotic ones. Finally, symbiotic females were less susceptible to an organophosphate insecticide, fenitrothion, yielding the lethal concentrations for 50 % (LC<sub>50</sub>) at 72.4 ppm and 92.4 ppm for apo-symbiotic and symbiotic groups, respectively. Taken together, our study demonstrates that the symbiotic effects on female <em>R. pedestris</em> would favor the host population growth.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 4","pages":"Article 102469"},"PeriodicalIF":1.3,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoemulsions of Leucaena leucocephala wood vinegar and main constituents exhibit high mosquitocidal activity against larvae of Culex pipiens L. (Diptera: Culicidae) via oxidative stress and neurotoxicity 白头翁木醋纳米乳剂及其主要成分通过氧化应激和神经毒性对淡纹库蚊幼虫表现出较高的杀蚊活性

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-02 DOI: 10.1016/j.aspen.2025.102471

Nehad E.M. Taktak , Noura A. Hassan , Hesham M. Aly , Mohamed E.I. Badawy

{"title":"Nanoemulsions of Leucaena leucocephala wood vinegar and main constituents exhibit high mosquitocidal activity against larvae of Culex pipiens L. (Diptera: Culicidae) via oxidative stress and neurotoxicity","authors":"Nehad E.M. Taktak , Noura A. Hassan , Hesham M. Aly , Mohamed E.I. Badawy","doi":"10.1016/j.aspen.2025.102471","DOIUrl":"10.1016/j.aspen.2025.102471","url":null,"abstract":"<div><div>The present study investigates the mosquitocidal efficacy and biochemical effect of <em>Leucaena leucocephala</em> wood vinegar, its major chemical constituents (2-methoxy-<em>p</em>-creosol and <em>o</em>-guaiacol), and their nanoemulsion formulations against <em>Culex pipiens</em> larvae. The larvicidal bioassay revealed that the products exhibited time- and concentration-dependent toxicity against larvae, with nanoemulsion formulations significantly enhanced efficacy, especially for 2-methoxy-<em>p</em>-cresol, which achieved an exceptionally low LC<sub>50</sub> of 0.09 mg/L after 48 h of exposure. Biochemical assays confirmed substantial alterations in larval enzyme activity, indicating oxidative stress and neurotoxic effects induced by the treatments. Gas chromatography–mass spectrometry (GC–MS) analysis identified key bioactive compounds, including 2-methoxy-<em>p</em>-creosol (18.48 % peak area) and <em>o</em>-guaiacol (17.32 % peak area), which were further evaluated through molecular docking against target enzymes acetylcholinesterase (AChE), adenosine triphosphatase (ATPase), glutathione S-transferase (GST), and carboxylesterase (CarE). Docking results demonstrated strong binding affinities, suggesting potential mechanisms of action. These findings highlight the potential of <em>L. leucocephala</em> wood vinegar and its nanoformulations as eco-friendly, plant-based mosquito control agents with promising bioactivity at both physiological and molecular levels.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102471"},"PeriodicalIF":1.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the feasibility of ethyl formate fumigation as a stand-alone phytosanitary treatment of Bactrocera dorsalis (Diptera: Tephritidae) in mandarin fruit 评价甲酸乙酯熏蒸单独处理桔小实蝇（双翅目：绢蝇科）的可行性

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-01 DOI: 10.1016/j.aspen.2025.102466

Tae‐Hyung Kwon , Dongbin Kim , Kyeongnam Kim , Gwang-Hyun Roh , Bong-Su Kim , Sung-Eun Lee , Jia-Wei Tay , Byung-Ho Lee , Dong H. Cha

{"title":"Evaluating the feasibility of ethyl formate fumigation as a stand-alone phytosanitary treatment of Bactrocera dorsalis (Diptera: Tephritidae) in mandarin fruit","authors":"Tae‐Hyung Kwon , Dongbin Kim , Kyeongnam Kim , Gwang-Hyun Roh , Bong-Su Kim , Sung-Eun Lee , Jia-Wei Tay , Byung-Ho Lee , Dong H. Cha","doi":"10.1016/j.aspen.2025.102466","DOIUrl":"10.1016/j.aspen.2025.102466","url":null,"abstract":"<div><div>Oriental fruit fly, <em>Bactrocera dorsalis</em>, is one of the most destructive quarantine pests of fruits and vegetables, with increasing incursions reported worldwide. During a <em>B. dorsalis</em> outbreak, all fruits and potential host materials within the quarantine area are destroyed to prevent further spread. As a first step toward developing an emergency treatment to allow fruit movement from a <em>B. dorsalis</em> quarantine area, we evaluated the feasibility of using ethyl formate (EF) fumigation as a stand-alone phytosanitary treatment against <em>B. dorsalis</em>. In EF efficacy trials with <em>B. dorsalis</em> eggs and larvae in the absence of fruit (naked conditions), eggs were the most tolerant life stage, with LCt<sub>99</sub> values—lethal concentration × time products that cause 99 % mortality—of 716.9 and 1,271.0 g h/m<sup>3</sup> at 20 and 4 °C, respectively, followed by first and third instar larvae. When <em>B. dorsalis</em> were artificially inoculated into mandarin fruit, significantly greater EF doses were necessary to control <em>B. dorsalis</em> inside fruit, with the LCt<sub>99</sub> values for eggs increased to 1,606.0 and 1,761.0 at 20 and 4 °C, respectively. Although these calculated EF doses were effective against <em>B. dorsalis</em>, they caused significant damage to quality of mandarins, including reduced sugar content and increased fruit decay. These findings indicate that EF fumigation alone is not a feasible stand-alone treatment for disinfesting <em>B. dorsalis</em> in mandarins. We discuss future research directions aimed at maintaining treatment efficacy while minimizing fruit damage by combining EF fumigation with other phytosanitary treatments.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102466"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first cixiid planthopper (Fulgoromorpha: Cixiidae) from Lower Cretaceous Jinju Formation (Republic of Korea) 韩国下白垩世晋州组第一种慈禧飞虱（狐形目：慈禧科）

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-01 DOI: 10.1016/j.aspen.2025.102472

Mathieu Boderau , Gi-Soo Nam , Corentin Jouault , André Nel

引用次数: 0

Polyadenylate-binding proteins are essential for the molting development of locusts 聚腺苷酸结合蛋白对蝗虫的蜕皮发育至关重要

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-01 DOI: 10.1016/j.aspen.2025.102470

Tao Li , Yayin Li , Weimin Liu , Jianzhen Zhang , Xiaoming Zhao

{"title":"Polyadenylate-binding proteins are essential for the molting development of locusts","authors":"Tao Li , Yayin Li , Weimin Liu , Jianzhen Zhang , Xiaoming Zhao","doi":"10.1016/j.aspen.2025.102470","DOIUrl":"10.1016/j.aspen.2025.102470","url":null,"abstract":"<div><div>Polyadenylate-binding proteins (PABPs) are critical for mRNA stability, translation, and decay in eukaryotes. However, their specific roles in regulating molt-related gene stability in insects remain unclear. Here, we characterize two PABP genes, LmPABP1 and LmPABP2, in the migratory locust, <em>Locusta migratoria</em>. Phylogenetic analysis revealed LmPABP1 as a cytoplasmic PABP (PABPC) and LmPABP2 as a nuclear PABP (PABPN). Tissue-specific expression profiling showed that <em>LmPABP1</em> is highly expressed in the integument and gut during the fifth instar, while <em>LmPABP2</em> expression exhibits stage-specific fluctuations. RNA interference-mediated knockdown of <em>LmPABP1</em> caused 100% mortality in both fourth- and fifth-instar nymphs, whereas silencing <em>LmPABP2</em> led to varied mortality during pre-, mid-, and post-molting stages. Histological analysis showed disrupted cuticle dissolution in <em>LmPABP1</em>-silenced locusts and delayed dissolution in <em>LmPABP2</em>-depleted individuals. Critically, <em>LmPABP1</em> knockdown significantly downregulated key chitin metabolism genes (<em>LmCht5</em>, <em>LmCht10</em>, and <em>LmUAP1</em>). Furthermore, actinomycin D treatment confirmed that LmPABP1 silencing accelerates decay of <em>LmCht5</em> and <em>LmCht10</em> mRNAs, indicating its essential role in mRNA stabilization. This study delineates the functional divergence of cytoplasmic LmPABP1 and nuclear LmPABP2 in post-transcriptional regulation, specifically stabilizing chitinase mRNAs during locust molting, offering potential molecular targets for developing novel pest control strategies.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102470"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repellent activity of nanocrystalline ammonium paratungstate on the German cockroach, Blattella germanica (L.) 仲钨酸铵纳米晶对德国小蠊的驱避作用

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-01 DOI: 10.1016/j.aspen.2025.102468

Ryusei Shimizu , Ikuko Ishikawa , Haruyuki Nakano , Yuuichi Ishikawa , Keiji Nakamura

{"title":"Repellent activity of nanocrystalline ammonium paratungstate on the German cockroach, Blattella germanica (L.)","authors":"Ryusei Shimizu , Ikuko Ishikawa , Haruyuki Nakano , Yuuichi Ishikawa , Keiji Nakamura","doi":"10.1016/j.aspen.2025.102468","DOIUrl":"10.1016/j.aspen.2025.102468","url":null,"abstract":"<div><div>Metal-based nanomaterials’ action as a repellent against the German cockroach, <em>Blattella germanica</em> (Blattodea: Ectobiidae), was examined. After the inner surfaces of black non-transparent rectangular plastic cases were sprayed with colloidal solutions of nanocrystalline ammonium paratungstate or pure water, two black cases were placed as shelters in a large container. Then adult cockroaches were introduced into the container. Cockroaches in shelters were counted 1–3 h later. When using boxes sprayed with 3.0 % colloidal solution and pure water, no cockroach that entered either of the shelters chose the shelter with nanocrystalline ammonium paratungstate. Even with concentrations lower than 3.0 %, fewer cockroaches chose shelters sprayed with the nanomaterial solution over those sprayed with pure water. The repellent effects persisted for 90 days and then decreased gradually. When cockroaches were reared in the container sprayed with the colloidal solution for a week, mortality was only 6 %, which was not different from that of the control experiment. Chemical insecticides in human dwellings pose a risk of human exposure. By contrast, a low impact on human health is expected from the non-volatile nanomaterial used for this study, which shows persistent repellency lasting for several months. Nanocrystalline ammonium paratungstate can be a strong tool when used as part of integrated pest management for cockroaches.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102468"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nesting habitat and prey preferences in two caterpillar-hunting Anterhynchium species (Hymenoptera: Vespidae: Eumeninae) 两种捕食毛虫的前翅蜂的筑巢生境和猎物偏好（膜翅目：蜂科：翅蜂科）

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-09-01 DOI: 10.1016/j.aspen.2025.102462

Misaki Tsujii , Tomoji Endo , Shinji Sugiura

{"title":"Nesting habitat and prey preferences in two caterpillar-hunting Anterhynchium species (Hymenoptera: Vespidae: Eumeninae)","authors":"Misaki Tsujii , Tomoji Endo , Shinji Sugiura","doi":"10.1016/j.aspen.2025.102462","DOIUrl":"10.1016/j.aspen.2025.102462","url":null,"abstract":"<div><div>Many wasps hunt prey and transport them to nests located far from prey-hunting sites. While many studies have presented either prey preferences or nesting habitat selection in hunting wasps, few have investigated whether prey habitats are closely linked to nesting habitats. To clarify the relationship between prey and nesting habitats, we studied the prey items and nesting habitats of the two caterpillar-hunting wasps, <em>Anterhynchium flavomarginatum</em> and <em>Anterhynchium gibbifrons</em> (Hymenoptera: Vespidae: Eumeninae), in a mixed agricultural and forest landscape in Hyogo, central Japan. Trap nests were placed in four environments, agricultural fields, bamboo forest edges, broad-leaved forests, and bamboo/broad-leaved forest boundaries, from June to November 2020. <em>Anterhynchium flavomarginatum</em> and <em>A. gibbifrons</em> nested in 25% and 60% of the 48 trap nests, respectively. The former nested in all four environments, whereas the latter nested at bamboo forest edges, in broad-leaved forests, and at the boundaries. <em>Anterhynchium flavomarginatum</em> provisioned its nests with larvae of 15 moth species (Lepidoptera: Crambidae, Pyralidae, Tortricidae), whose host plants were found in agricultural fields and other environments. In contrast, <em>A. gibbifrons</em> provisioned its nests exclusively with larvae of <em>Demobotys pervulgalis</em> (Crambidae), with its host plants restricted to bamboo. Our findings suggest that prey availability influences nesting habitat selection in the two <em>Anterhynchium</em> species. <em>Anterhynchium flavomarginatum</em> showed greater habitat flexibility, likely due to its broader prey range, whereas <em>A</em>. <em>gibbifrons</em> exhibited a narrow habitat range corresponding to the host plant distribution of its sole prey species.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102462"},"PeriodicalIF":1.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of sorbitol dehydrogenase in silkworm ovarian physiology and metabolism: insights from mutant analysis and proteomic profiling 山梨醇脱氢酶在家蚕卵巢生理和代谢中的作用：来自突变体分析和蛋白质组学分析的见解

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-08-27 DOI: 10.1016/j.aspen.2025.102467

Yangwei Ning , Yizhong Zhang , Yanrong Chen , Shanshan Wang , Shunming Tang , Juan Zhu , Xingjia Shen

{"title":"Role of sorbitol dehydrogenase in silkworm ovarian physiology and metabolism: insights from mutant analysis and proteomic profiling","authors":"Yangwei Ning , Yizhong Zhang , Yanrong Chen , Shanshan Wang , Shunming Tang , Juan Zhu , Xingjia Shen","doi":"10.1016/j.aspen.2025.102467","DOIUrl":"10.1016/j.aspen.2025.102467","url":null,"abstract":"<div><div>Sorbitol dehydrogenase (SDH) plays a pivotal role in the polyol pathway by regulating sorbitol and fructose metabolism. During silkworm (<em>Bombyx mori</em>) embryonic development, SDH catalyzes the conversion of sorbitol to glycogen to meet the metabolic demands of the growing embryo. However, its function in the ovarian physiology and metabolism remains poorly understood. In this study,we generated a homozygous BmSDH mutant strain using CRISPR/Cas9 and performed TMT-based proteomic analysis to investigate the impact of SDH knockout on the ovarian proteome at the 3rd-day pupal stage. Concurrently, we quantified glycogen, sorbitol, glycerol, and trehalose levels in mutant ovarian tissues to assess metabolic perturbations. The results revealed that a 6-base insertion in BmSDH disrupted enzymatic function. Proteomic profiling identified 204 differentially expressed proteins (DEPs) (124 upregulated, 80 downregulated), enriched in 90 GO terms and 107 KEGG pathways. Among the DEPs, 19 key proteins were identified, including Six downregulated proteins involved in amino acid/glucose metabolism indicated reduced metabolic flux in mutants. Eight proteins associated with growth, and development showed differential expression, with six upregulated and two downregulated, implicated BmSDH in ovarian development and diapause initiation. Furthermore, Five disease-associated proteins (e.g., cardiomyopathy-linked factors) revealed evolutionarily conserved BmSDH functions. Metabolomics showed elevated glycogen, trehalose, and glycerol in mutant ovaries, consistent with disrupted polyol pathway dynamics. These results elucidate BmSDH’s multifaceted roles in silkworm ovarian development, bridging metabolic regulation, reproductive programming, and conserved disease mechanisms.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102467"},"PeriodicalIF":1.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and expression analysis of olfactory genes from Ephestia elutella (Lepidoptera: Pyralidae) antennal transcriptome elutella（鳞翅目：皮蝇科）嗅觉基因转录组的鉴定与表达分析

IF 1.3 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-08-26 DOI: 10.1016/j.aspen.2025.102460

Guiyao Wang , Yueliang Bai , Hanping Zhou , Yingjie Liu , Taibo Liang , Keke Sun , Yongsheng Wang , Qiang Xu , Jianwei Wang

{"title":"Identification and expression analysis of olfactory genes from Ephestia elutella (Lepidoptera: Pyralidae) antennal transcriptome","authors":"Guiyao Wang , Yueliang Bai , Hanping Zhou , Yingjie Liu , Taibo Liang , Keke Sun , Yongsheng Wang , Qiang Xu , Jianwei Wang","doi":"10.1016/j.aspen.2025.102460","DOIUrl":"10.1016/j.aspen.2025.102460","url":null,"abstract":"<div><div><em>Ephestia elutella</em> (Lepidoptera: Pyralidae) is a globally significant pest of stored products. Its olfactory system is crucial for mating, oviposition, and host location. Despite its importance, the mechanisms underlying odorant recognition in <em>E. elutella</em> are not well understood. Olfactory genes are thought to initiate the process of olfactory signal transduction in insects. In this study, we conducted antennal transcriptome sequencing of <em>E. elutella</em>, identifying 75 genes related to olfaction. This list includes 9 odorant-binding proteins (<em>OBPs</em>), 4 chemosensory proteins (<em>CSPs</em>), 46 olfactory receptors (<em>ORs</em>), 6 ionotropic receptors (<em>IRs</em>), 7 gustatory receptors (<em>GRs</em>), and 3 sensory neuron membrane proteins (<em>SNMPs</em>). Results from quantitative real-time PCR indicated that 7 <em>EeluOBPs</em>, 38 <em>EeluORs</em> and 5 <em>EeluGRs</em> exhibited higher expression levels in male antennae compared to females. These insights lay the groundwork for future research into the chemoreceptive mechanisms of <em>E. elutella</em>.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 3","pages":"Article 102460"},"PeriodicalIF":1.3,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0