Journal of Asia-pacific Entomology最新文献_第2页

Comparative analysis of fecundity related traits and transcriptome in two isofemale lines of the egg parasitoid, Trichogramma dendrolimi (Matsumura) 卵寄生虫树枝金龟子（松村）两个异雌品系繁殖力相关性状和转录组的比较分析

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-03-01 DOI: 10.1016/j.aspen.2025.102387

Hong-Yu Liang , An-Ke Liu , Ning He , Jin-Cheng Zhou , Xiao-Hui Ma , Li-Sheng Zhang , Wu-Nan Che , Hui Dong

{"title":"Comparative analysis of fecundity related traits and transcriptome in two isofemale lines of the egg parasitoid, Trichogramma dendrolimi (Matsumura)","authors":"Hong-Yu Liang , An-Ke Liu , Ning He , Jin-Cheng Zhou , Xiao-Hui Ma , Li-Sheng Zhang , Wu-Nan Che , Hui Dong","doi":"10.1016/j.aspen.2025.102387","DOIUrl":"10.1016/j.aspen.2025.102387","url":null,"abstract":"<div><div>The egg parasitoid, <em>Trichogramma dendrolimi</em>, plays an important role as a biological control agent for the control of various lepidopteran pests. The reproductive capacity of <em>T. dendrolimi</em> is essential for both mass-rearing and effective pest control. In this study, a <em>T. dendrolimi</em> line with a high fecundity trait (Td-H) and another with a low fecundity trait (Td-L) were selected from 13 isofemale lines collected across different regions of China. The fecundity trait of Td-H and Td-L females was validated through assessments of oocyte number in the ovary, fecundity, body size, and adult longevity. To investigate the expression patterns of fecundity-related genes in <em>T. dendrolimi</em>, transcriptomes of Td-H and Td-L females were sequenced and compared. A total of 441 differentially expressed genes (DEGs), including 181 up-regulated genes and 260 down-regulated genes, were identified. Moreover, 11 genes were specifically expressed in Td-H females, while 16 genes were exclusive to Td-L females. These DEGs were mainly enriched in categories such as “metabolic process” (102 DEGs), “organelle”(53 DEGs), “catalytic activity” (90 DEGs), and “binding” (174 DEGs) in GO analysis, as well as various metabolic pathways (58 DEGs) and the “Neuroactive ligand-receptor interaction” pathway (22 DEGs) in KEGG analysis. The accuracy of seven up-regulated DEGs and seven down-regulated DEGs in the transcriptome was confirmed using RT-qPCR. These findings will be helpful for investigating the regulatory mechanisms associated with the fecundity traits of <em>T. dendrolimi</em> and provide a reference for improving biological control agents against pests.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102387"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematicidal potential of berberine and sanguinarine from Chelidonium majus against Meloidogyne incognita

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-26 DOI: 10.1016/j.aspen.2025.102384

Fangwei Lu , Zixuan Wang , Youchuang Chang , Dandan Cao , Guixia Liu

{"title":"Nematicidal potential of berberine and sanguinarine from Chelidonium majus against Meloidogyne incognita","authors":"Fangwei Lu , Zixuan Wang , Youchuang Chang , Dandan Cao , Guixia Liu","doi":"10.1016/j.aspen.2025.102384","DOIUrl":"10.1016/j.aspen.2025.102384","url":null,"abstract":"<div><div>In this study, we investigated the effects of <em>Chelidonium majus</em> and its main alkaloids on the activity of <em>Meloidogyne incognita</em>. The dipping method evaluated the effects of <em>C. majus</em> crude extracts of the above- and belowground parts(ACE and BCE) and main alkaloids at different concentrations and durations on egg hatching and the second-stage juveniles (J2s) activity. The results revealed that ACE and BCE of C. majus, berberine and sanguinarine, have inhibitory effects on J2s and egg hatching. J2s mortality and egg hatching inhibition increased with higher crude extract and alkaloid concentrations and longer treatment time. At 90 mg/mL for 48 h, ACE and BCE resulted in J2s mortalities of 89.01 % and 97.43 %, and egg hatching inhibition rates of 100.00 % and 98.67 %. The LC<sub>50</sub> of ACE is 24.42 mg/mL, and the LC<sub>50</sub> of BCE is 20.75 mg/mL. Exposed to a treatment of 2 mg/mL for 24 h, the corrected mortality rates for berberine and sanguinarine were 90.48 % and 92.08 %, with their LC<sub>50</sub> values being 0.102 and 0.089 mg/mL, demonstrating significant nematicidal activity. Berberine and sanguinarine have egg hatching inhibition rates of 94.55 % and 87.13 %. Furthermore, we determined the absolute contents of berberine and sanguinarine in the above- and belowground parts of <em>C. majus.</em> The contents of berberine and sanguinarine base in the aboveground parts were 14.65 and 79.33 µg/g, while the underground parts was 180.26 and 714.06 µg /g. Our results indicate that <em>C. majus</em> crude extract and their potent alkaloids hold potential as natural nematicides for controlling <em>M. incognita.</em></div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102384"},"PeriodicalIF":1.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical compositions and insecticidal activities of Clausena lenis (Rutaceae) and Clausena excavata (Rutaceae) essential oils against two stored-product insects

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-24 DOI: 10.1016/j.aspen.2025.102388

Yu-Chen Zhang , Ao Liu , Yuan Zhang , Yong-Qing Gu , Shu-Shan Du

{"title":"Chemical compositions and insecticidal activities of Clausena lenis (Rutaceae) and Clausena excavata (Rutaceae) essential oils against two stored-product insects","authors":"Yu-Chen Zhang , Ao Liu , Yuan Zhang , Yong-Qing Gu , Shu-Shan Du","doi":"10.1016/j.aspen.2025.102388","DOIUrl":"10.1016/j.aspen.2025.102388","url":null,"abstract":"<div><div><em>Tribolium castaneum</em> Herbst, 1797 (Coleoptera: Tenebrionidae) and <em>Lasioderma serricorne</em> Fabricius, 1792 (Coleoptera: Anobiidae) are two globally prevalent stored-product pests. Traditional management strategies primarily involve the use of synthetic insecticides and repellents. However, plant essential oils (EOs) pose lower risks to human health and ecological environment. This study focused on two <em>Clausena</em> species plants, <em>Clausena lenis</em> and <em>Clausena excavata</em>, exploring the insecticidal and repellent properties of EOs extracted from different parts of them against <em>T. castaneum</em> and <em>L. serricorne</em>. The EOs were extracted via hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC–MS). A total of 57 components were identified. The EOs from the branches and leaves of two <em>Clausena</em> plants were primarily composed of sesquiterpenes, while <em>C. lenis</em> fruits EO was mainly composed of monoterpenes (58.79 %) and esters (38.95 %). <em>Clausena lenis</em> leaves EO demonstrated the highest contact toxicity against <em>T. castaneum</em>, with a mortality rate of 72 % at a concentration of 50 %, while <em>C. lenis</em> branches EO was particularly effective against <em>L. serricorne</em>, with an LD<sub>50</sub> of 8.24 mg/L air. In addition, <em>C. lenis</em> fruits EO exhibited the best repellent effect against these two pests. According to these results, <em>C. lenis</em> and <em>C. excavata</em> show promise to be developed into eco-friendly botanical insecticides for the prevention and control of stored-product pests, especially for application in agricultural storage. These findings highlight the potential for these EOs to serve as sustainable alternatives to synthetic insecticides, offering a safer and environmentally friendly approach to pest management in stored agricultural products.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102388"},"PeriodicalIF":1.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promotional activity of an uncharacterized protein Bm0979 on Nosema bombycis in silkworm, bombyx mori 未定性蛋白质 Bm0979 对家蚕蚕蛾的促进作用

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-24 DOI: 10.1016/j.aspen.2025.102389

Yujia Fang , Qiong Yu , Wenzhe Chen , Zhenghao Han , Qingsheng Qu , Zhenwei Jia , Shidong Jiang , Jie Cheng , Xudong Tang , Ping Qian

{"title":"Promotional activity of an uncharacterized protein Bm0979 on Nosema bombycis in silkworm, bombyx mori","authors":"Yujia Fang , Qiong Yu , Wenzhe Chen , Zhenghao Han , Qingsheng Qu , Zhenwei Jia , Shidong Jiang , Jie Cheng , Xudong Tang , Ping Qian","doi":"10.1016/j.aspen.2025.102389","DOIUrl":"10.1016/j.aspen.2025.102389","url":null,"abstract":"<div><div>Microsporidia are intracellular parasites that infect a diverse range of hosts, including economically significant insects like the silkworm (<em>Bombyx mori</em>). In this study, we investigated the effects of the microsporidian pathogen <em>Nosema bombycis (Nb)</em> on <em>B. mori</em>, with a focus on the cloning and functional characterization of the gene <em>Bm0979</em>. Our findings indicate that <em>Bm0979</em> has at least two isoforms and is significantly upregulated in response to <em>Nb</em> infection. Functional assays involving overexpression and knockdown of <em>Bm0979</em> in silkworm cells revealed that this gene promotes <em>Nb</em> proliferation, identifying it as a potential target for enhancing silkworm resistance to pebrine disease. Transcriptome sequencing further revealed that Bm0979 modulates the expression of genes related to the PI3K/Akt signaling pathway, which is implicated in immune responses and cellular metabolism. These findings provide new insights into the molecular mechanisms underlying host-pathogen interactions in silkworms and could contribute to the development of strategies for controlling microsporidia infections in sericulture.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102389"},"PeriodicalIF":1.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-22 DOI: 10.1016/j.aspen.2025.102386

Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin

{"title":"The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)","authors":"Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin","doi":"10.1016/j.aspen.2025.102386","DOIUrl":"10.1016/j.aspen.2025.102386","url":null,"abstract":"<div><div>The silk of silkworm, <em>Bombyx mori</em>, consists mainly of proteins, which contain some small proteins with specialized functions that confer special properties to silk, such as antimicrobial properties. Serine protease inhibitors (SPIs) are one of them, and the types that have been identified are seroin, serpin, Kunitz type, and Kazal type, but the function of the Kunitz/Kazal type is not known. As compared to others, Kazal molecular weight is relatively minimal. In this study, we focused on the function of a Kazal-type inhibitor BmSPI2, and its sequence characterization and expression profile were first refined. To clarify its function, BmSPI2 recombinant protein was expressed in sf9 cells using baculovirus expression system. Further testing revealed that BmSPI2 could inhibit trypsin, <em>α-</em>chymotrypsin, and plasmin, whereas has only some effect on proteinase K. This suggests that BmSPI2 has significant serine protease inhibitory activity. In-depth analysis revealed that BmSPI2 also effectively inhibited fibrinogen degradation mediated by fibrinolytic enzymes. Finally, we examined its antimicrobial-related functions using prepared antibodies and found that BmSPI2 was also able to directly bind to bacteria and fungi to inhibit their proliferation. The results of the study enriched the function of BmSPI2 and laid a theoretical foundation for its development and utilization.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102386"},"PeriodicalIF":1.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and virulence of three strains of Beauveria bassiana from silkworms, Bombyx mori

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-17 DOI: 10.1016/j.aspen.2025.102385

Qiang Zhang , Wenqing Lai , Qin Li , Xiaoying Zhang , Zhan-peng Lu , Jiawei Jiang , Wenjing Yu , Zuo-min Shao , Xue-yang Wang , Mu-wang Li

{"title":"Isolation, identification, and virulence of three strains of Beauveria bassiana from silkworms, Bombyx mori","authors":"Qiang Zhang , Wenqing Lai , Qin Li , Xiaoying Zhang , Zhan-peng Lu , Jiawei Jiang , Wenjing Yu , Zuo-min Shao , Xue-yang Wang , Mu-wang Li","doi":"10.1016/j.aspen.2025.102385","DOIUrl":"10.1016/j.aspen.2025.102385","url":null,"abstract":"<div><div><em>Beauveria bassiana</em> (<em>B. bassiana</em>) is a fungus widely used in the production of traditional Chinese medicine <em>Bombyx batryticatus</em> (<em>B. batryticatus</em>) and in the control of agricultural and forestry pests, keeping on discovering new strains with better vitality, virulence and proliferation will be favorable to promote its continuous promotion in production. The aim of this study is to discover and identify new <em>B. bassiana</em> strains from the natural environment of silkworm breeding and to enrich and update its resource base. In the process of preserving the genetic resources of silkworms, we collected a total of 52 <em>B. bassiana</em> samples, and successfully isolated and identified three strains, Bb10B, Bb01J, and Bb01S, through morphological and modern subsystematic evolutionary analyses. The virulence assay showed that strain Bb10B had the highest rate of disease and lethality, with a half lethal time (LT<sub>50</sub>) of only 6.40 days, and had rapid growth and high spore production. Further studies showed that the virulence of Bb10B against silkworm larvae increased significantly with increasing concentration in the concentration range of 1.2 × 10<sup>5</sup> to 1.2 × 10<sup>8</sup> spores/mL, with the median lethal concentration (LC<sub>50</sub>) of 3.99 × 10<sup>5</sup> spores/mL. Pathological section analysis of the midgut of silkworms showed that the cells of the midgut of the silkworms were sparsely arranged and locally broken after 4 d of infection. Therefore, this study enriches the germplasm resources of <em>B. bassiana</em> and provides a good basis for promoting its application in <em>B. batryticatus</em> production and pest biological control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 2","pages":"Article 102385"},"PeriodicalIF":1.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic note of the family Lucanidae (Coleoptera: Scarabaeoidea) in Cambodia

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-12 DOI: 10.1016/j.aspen.2025.102383

Eunjoong Kim , Taeyeong Kwon , Eun Young Choi , Jong Bong Choi , Myeonghwan Kim , Sodavy Gnim , Jong Kyun Park

引用次数: 0

Novel splice variants of the cytochrome P450 9A19 gene in the domestic silkworm Bombyx mori

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-10 DOI: 10.1016/j.aspen.2025.102382

Jin Ha Yun, Seung-Won Park

{"title":"Novel splice variants of the cytochrome P450 9A19 gene in the domestic silkworm Bombyx mori","authors":"Jin Ha Yun, Seung-Won Park","doi":"10.1016/j.aspen.2025.102382","DOIUrl":"10.1016/j.aspen.2025.102382","url":null,"abstract":"<div><div><em>CYP9</em> gene family members participate in neutralization pathways associated with pesticide resistance. Alternative splicing ultimately plays a role in increasing the diversity of the proteome through increasing transcriptome diversity. Here, we characterized various novel <em>CYP9A19</em> isoforms in <em>Bombyx mori</em>. We first identified an exon 4 deletion isoform that was 203 bp smaller than expected due to a deletion of exon 4 from the protein coding sequence (CDS) region of the <em>CYP9A19</em> cDNA. Within the <em>CYP9A19</em> cDNA, there is a 176 bp 5′-untranslated region (UTR); reverse transcription-polymerase chain reaction results showed 74 bp lower and 122 bp higher molecular-weight products compared with the expected size from the 5′-UTR of <em>CYP9A19</em> cDNA in <em>B. mori</em>. Additionally, we found a new sequence (5′-GTCTCAGGTTCAGGGCTCTAGCAATTTTTCACAG-3′) in the 5′-UTR of <em>CYP9A19</em>. Currently, our understanding of the various <em>CYP9A19</em> isoform functions in pesticide degradation and resistance and insect development and differentiation stages remains limited. Therefore, additional studies on the alternative splicing process are needed to verify the biological role of the novel splicing variant of <em>CYP9A19</em> in <em>B. mori</em>. Our findings may provide insight into the genetic background of the processes and mechanisms underlying the stages of insect development and differentiation in lepidoptera species.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102382"},"PeriodicalIF":1.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two new species of the planthopper genus Pitambara Distant, 1906 (Hemiptera, Fulgoromorpha, Lophopidae) from Yunnan Province of China

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-06 DOI: 10.1016/j.aspen.2025.102380

Shasha Lv , Lin Yang , Yubo Zhang , Xiangsheng Chen

引用次数: 0

A remarkable new species of the genus Dichodontus Burmeister, 1847 (Coleoptera: Scarabaeidae: Dynastinae) from Thailand and Myanmar, with discussions on some Sundaic species

IF 1.1 3区农林科学

Journal of Asia-pacific Entomology Pub Date : 2025-02-04 DOI: 10.1016/j.aspen.2025.102381

Georgi A. Geshev , Wuttipon Pathomwattananurak , Kawin Jiaranaisakul

引用次数: 0