Identification and expression analysis of olfactory genes from Ephestia elutella (Lepidoptera: Pyralidae) antennal transcriptome

IF 1.3 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology Pub Date : 2025-08-26 DOI:10.1016/j.aspen.2025.102460

Guiyao Wang , Yueliang Bai , Hanping Zhou , Yingjie Liu , Taibo Liang , Keke Sun , Yongsheng Wang , Qiang Xu , Jianwei Wang

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Abstract

Ephestia elutella (Lepidoptera: Pyralidae) is a globally significant pest of stored products. Its olfactory system is crucial for mating, oviposition, and host location. Despite its importance, the mechanisms underlying odorant recognition in E. elutella are not well understood. Olfactory genes are thought to initiate the process of olfactory signal transduction in insects. In this study, we conducted antennal transcriptome sequencing of E. elutella, identifying 75 genes related to olfaction. This list includes 9 odorant-binding proteins (OBPs), 4 chemosensory proteins (CSPs), 46 olfactory receptors (ORs), 6 ionotropic receptors (IRs), 7 gustatory receptors (GRs), and 3 sensory neuron membrane proteins (SNMPs). Results from quantitative real-time PCR indicated that 7 EeluOBPs, 38 EeluORs and 5 EeluGRs exhibited higher expression levels in male antennae compared to females. These insights lay the groundwork for future research into the chemoreceptive mechanisms of E. elutella.

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elutella（鳞翅目：皮蝇科）嗅觉基因转录组的鉴定与表达分析

elutella（鳞翅目：皮蝇科）是全球重要的储粮害虫。它的嗅觉系统对交配、产卵和寄主定位至关重要。尽管它很重要，但对臭鼬嗅觉识别的机制尚不清楚。嗅觉基因被认为启动了昆虫的嗅觉信号转导过程。在这项研究中，我们对elutella进行了触角转录组测序，鉴定了75个与嗅觉相关的基因。该列表包括9种气味结合蛋白（OBPs）， 4种化学感觉蛋白（CSPs）， 46种嗅觉受体（ORs）， 6种嗜离子受体（IRs）， 7种味觉受体（GRs）和3种感觉神经元膜蛋白（snp）。实时荧光定量PCR结果显示，7个EeluOBPs、38个EeluORs和5个eelugr在雄性触角中的表达量高于雌性。这些发现为进一步研究艾草的化学接受机制奠定了基础。

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来源期刊

Journal of Asia-pacific Entomology Agricultural and Biological Sciences-Insect Science

CiteScore

2.70

自引率

6.70%

发文量

152

审稿时长

69 days

期刊介绍： The journal publishes original research papers, review articles and short communications in the basic and applied area concerning insects, mites or other arthropods and nematodes of economic importance in agriculture, forestry, industry, human and animal health, and natural resource and environment management, and is the official journal of the Korean Society of Applied Entomology and the Taiwan Entomological Society.