Knockdown of the juvenile hormone acid O-methyltransferase gene impairs development of Agrotis ipsilon (Lepidoptera: Noctuidae)

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology Pub Date : 2025-04-13 DOI:10.1016/j.aspen.2025.102410

Jin Zhang , Jia-Min Yu , Xiao-Xiao Shi , Dong-Yang Liu , Quan Deng , Jiang-Nan Peng , Mao-Ye Li , Su Liu

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Abstract

In insects, the juvenile hormone (JH) acid O-methyltransferase (JHAMT) is required for JH synthesis. Agrotis ipsilon is known as the black cutworm and is a key agricultural pest; however, its JHAMT gene remains uncharacterized. Herein, we identified and functionally characterized A. ipsilon JHAMT (designated AiJHAMT). AiJHAMT consisted of three introns and four exons, and encoded the AiJHAMT protein, which contained 271 amino acid residues. AiJHAMT displayed features typical of other insect JHAMTs and shared a close phylogenetic relationship with a Mythimna separata ortholog. Experimentally, in A. ipsilon larvae, oral double-stranded RNA (dsRNA) delivery significantly reduced AiJHAMT mRNA levels and JH titers. AiJHAMT knockdown also affected larval growth, sharply reducing larval body length and weight and AiJHAMT-silenced larvae survival rates decreased to 46.5%. Moreover, AiJHAMT silencing reduced pupation rates by 61.8%. Therefore, AiJHAMT is vital for A. ipsilon growth, development, and survival and may be used for RNA interference (RNAi)-based pest strategies.

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幼体激素酸o -甲基转移酶基因的敲低对小夜蛾发育的影响

在昆虫中，幼体激素（JH）酸o -甲基转移酶（JHAMT）是JH合成所必需的。黑穗病（Agrotis ipsilon）被称为黑蛾，是一种重要的农业害虫；然而，其JHAMT基因仍未被表征。在此，我们鉴定和功能表征了A. ipsilon JHAMT（指定为AiJHAMT）。AiJHAMT由3个内含子和4个外显子组成，编码了含有271个氨基酸残基的AiJHAMT蛋白。AiJHAMT表现出其他昆虫jhamt的典型特征，并与Mythimna separata同源物具有密切的系统发育关系。实验结果表明，口服双链RNA （dsRNA）可显著降低伊氏依蚊幼虫AiJHAMT mRNA水平和JH滴度。AiJHAMT基因敲除也影响了幼虫的生长，使幼虫体长和体重急剧下降，AiJHAMT基因沉默的幼虫存活率下降到46.5%。此外，AiJHAMT沉默使化蛹率降低了61.8%。因此，AiJHAMT对A. ipsilon的生长、发育和存活至关重要，可能用于基于RNA干扰（RNAi）的害虫策略。

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来源期刊

Journal of Asia-pacific Entomology Agricultural and Biological Sciences-Insect Science

CiteScore

2.70

自引率

6.70%

发文量

152

审稿时长

69 days

期刊介绍： The journal publishes original research papers, review articles and short communications in the basic and applied area concerning insects, mites or other arthropods and nematodes of economic importance in agriculture, forestry, industry, human and animal health, and natural resource and environment management, and is the official journal of the Korean Society of Applied Entomology and the Taiwan Entomological Society.