Journal of Bioactive and Compatible Polymers最新文献

{"title":"Wound healing and anti-inflammatory effects of Anethum graveolens extract loaded in PVA fibers: An in vitro and in vivo study","authors":"Linli Li, Fengjuan Wang","doi":"10.1177/08839115221104074","DOIUrl":"https://doi.org/10.1177/08839115221104074","url":null,"abstract":"Anethum graveolens extract (AGE) is known for its anti-inflammatory, antioxidative, and antibacterial activities. As wound infection, hyperactivity of inflammatory responses, and high oxidative stress are the leading causes of delayed wound healing, we were encouraged to design a delivery vehicle for AGE to develop a potential wound dressing material. In the current study, AGE was incorporated into the polyvinyl alcohol (PVA) scaffolds matrix via the electrospinning method. Various characterization methods were applied to assess the physicochemical and biological properties of the dressings. Cell culture studies with fibroblast cell line showed that AGE-loaded dressings could significantly promote cell viability under normal and oxidative stress conditions. The prepared wound dressings’ wound healing and anti-inflammatory properties were investigated on an excisional injury rat model. Wound healing assay showed that AGE-delivering wound dressings could significantly improve the wound healing response, as evidenced by a significantly higher rate of wound closure, epithelial thickness, and collagen deposition. Gene expression analysis revealed that the produced dressings downregulated inflammation-associated genes such as IL-1β and NFK-β. This preliminary research suggests the potential applicability of AGE-loaded PVA dressings in the clinic.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"160 1","pages":"299 - 315"},"PeriodicalIF":1.7,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77852695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo evaluation of porous alginate hydrogel containing retinoic acid for skin wound healing applications","authors":"Simin Nazarnezhad, M. Salehi, H. Samadian, Arian Ehtermi, N. Kasaiyan, H. Khastar, Ghasem Abbaszadeh-Goudarzi, Nariman Rezaei Kolarijani, Hodays Yeganehfard, H. Ziaei","doi":"10.1177/08839115221104071","DOIUrl":"https://doi.org/10.1177/08839115221104071","url":null,"abstract":"The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"115 1","pages":"332 - 342"},"PeriodicalIF":1.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87817394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ forming gelatin: Cyclodextrin hydrogels prepared by “click chemistry” to improve the sustained release of hydrophobic drugs","authors":"Phuong Le Thi, T. Tran, H. Luu, Dieu Linh Tran, T. H. Thi, D. Nguyen","doi":"10.1177/08839115221098058","DOIUrl":"https://doi.org/10.1177/08839115221098058","url":null,"abstract":"Injectable hydrogels offer a wide range of attractive benefits in drug delivery applications, such as non-invasive administration, easy drug incorporation and locally controlled release at the target sites. Herein, we designed a simple and efficient method to prepare injectable hydrogels composed of gelatin and cyclodextrin (CD) for high loading capacity of hydrophobic drugs. The hydrogels were formed by thiol-functionalized gelatin (GSH) and βCD-vinyl sulfone (βCD-VS) as cross-linker, via thiol-ene “click” chemistry. Hydrogels comprising of different cross-linker feed amount were investigated in terms of their physico-chemical properties, such as gelation time, mechanical strength, swelling ratio, porosity and degradation rates. For the use as a drug delivery vehicle, dexamethasone (DEX), a commonly anti-inflammatory, immunosuppressive but poorly water soluble drug was chosen to show the high drug loading capacity and prolonged drug release of hydrogels. The drug release was found to be depended on the concentration of βCD-VS due to the drug-CD interaction. In vitro cytotoxicity experiment also showed the cell compatibility of these hydrogels against human dermal fibroblasts. In summary, we expect this gelatin-CD “click” hydrogel will be a promising candidate for localized and long-term delivery of hydrophobic drugs. Graphical Abstract","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"36 12 1","pages":"252 - 266"},"PeriodicalIF":1.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90664018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CMC-based hydrogels loaded with Hypericum perforatum nanoemulsion for potential wound dressing applications","authors":"M. Moradi, Aboulfazl Barati, S. Moradi, M. Arjomandzadegan","doi":"10.1177/08839115221098059","DOIUrl":"https://doi.org/10.1177/08839115221098059","url":null,"abstract":"Wound healing is a complicated process requiring appropriate environment to accelerate healing process. In the recent years, many wound dressings have been developed for treating various kinds of wounds. In this study, we aimed to develop a novel dressing with high ability of burn wound healing and minimum side effects. Carboxymethyl cellulose (CMC) based hydrogels containing Hypericum perforatum were developed by grafting methacrylic acid and acrylamide onto CMC to produce a good mechanical strength dressing. Covalent crosslinking, which is responsible for stable mechanical structure, led to a 3D structure with appropriate water vapor transmission rate (2950 g/m2/day), controlled drug release (33% in 78 h), and great burn healing ability (almost complete healing in 10 day). The hydrogel has proper antimicrobial activity against the tested microorganisms. Zone of inhibition against E.coli was the higher in comparison with S. aureus and Candida. Minimum inhibitory concentration (MIC) for C. albicans, S. aureus, and E. coli were as 6, 4, and 5 mg/ml of H. perforatum. In vivo experiments on rats revealed that wound healing process by loaded hydrogels was faster in comparison with control group. All the results indicated that prepared hydrogel has the capability to accelerate burn wound healing process.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"5 1","pages":"316 - 331"},"PeriodicalIF":1.7,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89249153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Galloylated proanthocyanidins in dentin matrix exhibit biocompatibility and induce differentiation in dental stem cells.","authors":"Daniel Kulakowski,&nbsp;Rasika M Phansalkar,&nbsp;Ariene A Leme-Kraus,&nbsp;James McAlpine,&nbsp;Shao-Nong Chen,&nbsp;Guido F Pauli,&nbsp;Sriram Ravindran,&nbsp;Ana K Bedran-Russo","doi":"10.1177/08839115221095154","DOIUrl":"https://doi.org/10.1177/08839115221095154","url":null,"abstract":"<p><strong>Aim: </strong>Grape seed extract contains a complex mixture of proanthocyanidins (PACs), a plant biopolymer used as a biomaterial to improve reparative and preventive dental therapies. Co-polymerization of PACs with type I collagen mechanically reinforces the dentin extracellular matrix. This study assessed the biocompatibility of PACs from grape seed extract on dental pulp stem cells (DPSCs) in a model simulating leaching through dentin to the pulp cavity. The aim was to determine the type of PACs (galloylated vs. non-galloylated) within grape seed extract that are most compatible with dental pulp tissue.</p><p><strong>Methodology: </strong>Human demineralized dentin was treated with selectively-enriched dimeric PACs prepared from grape seed extract using liquid-liquid chromatography. DPSCs were cultured within a 2D matrix and exposed to PAC-treated dentin extracellular matrix. Cell proliferation was measured using the MTS assay and expression of odontoblastic genes was analyzed by qRT-PCR. Categorization of PACs leaching from dentin was performed using HPLC-MS.</p><p><strong>Results: </strong>Enriched dimeric fractions containing galloylated PACs increased the expression of certain odontoblastic genes in DPSCs, including Runt-related transcription factor 2 (RUNX2), vascular endothelial growth factor (VEGF), bone morphogenetic protein 2 (BMP2), basic fibroblast growth factor (FGF2), dentin sialophosphoprotein (DSPP) and collagen, type I, alpha 1 (COLI). Galloylated dimeric PACs also exhibited minor effects on DPSC proliferation, resulting in a decrease compared to control after five days of treatment. The non-galloylated dimer fraction had no effect on these genes or on DPSC proliferation.</p><p><strong>Conclusions: </strong>Galloylated PACs are biocompatible with DPSCs and may exert a beneficial effect on cells within dental pulp tissue. The observed increase in odontoblastic genes induced by galloylated PACs together with a decrease in DPSC proliferation is suggestive of a shift toward cell differentiation. This data supports the use of dimeric PACs as a safe biomaterial, with galloylated dimeric PACs exhibiting potential benefits to odontoblasts supporting dentin regeneration.</p>","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"37 3","pages":"220-230"},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353770/pdf/nihms-1857444.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9839823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coating of silver nanoparticles on polyurethane film surface by green chemistry approach and investigation of antibacterial activity against S. epidermidis","authors":"Filiz Kara, E. Aksoy, S. Aksoy, N. Hasirci","doi":"10.1177/08839115221098056","DOIUrl":"https://doi.org/10.1177/08839115221098056","url":null,"abstract":"Silver nanoparticles with potential antibacterial properties are included in biomaterials for the production of medical devices, which are used for diagnoses or treatment purposes. The aim of the current study was coating the polyurethane (PU) films with silver nanoparticles (AgNPs) due to their antibacterial efficacy. PU films were first modified by chitosan (CH), treated with AgNO3 to let CH chelate with silver ions, and then treated with vitamin-C (vit C) or glucose (Glu) to reduce the adsorbed ions to atomic silver to form AgNPs. The surfaces of the films were examined by ATR-FTIR, XPS, XRD, and SEM. Chemical bond formation between CH and Ag ions and AgNPs were determined by ATR-FTIR. Meanwhile, XPS and SEM analyses proved the presence of reduced metallic silver and nanoparticles on the film surfaces, respectively. According to the SEM analyses, a homogeneous distribution of AgNPs, with sizes 99–214 nm and 37–54 nm, on the film surfaces were obtained depending on Glu or vit C reduction, respectively. The films presented excellent antibacterial performance against Gram positive Staphylococcus epidermidis (S. epidermidis). These results suggested that the mentioned green technology can be easily applied to obtain AgNP coated polymeric surfaces with very high antibacterial efficacy. Although there are some studies dealing with AgNP formation on PU sponges or fibers, to the best of our knowledge, this is the first study showing AgNP formation on the CH conjugated PU films.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"8 1","pages":"205 - 219"},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84986669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delivery of miRNA-126 through folic acid-targeted biocompatible polymeric nanoparticles for effective lung cancer therapy","authors":"Forough N Golafzani, A. Vaziri, M. Javanmardi, Fatemeh Seyfan, M. Yazdanifar, Sepideh Khaleghi","doi":"10.1177/08839115221095152","DOIUrl":"https://doi.org/10.1177/08839115221095152","url":null,"abstract":"Objective: Nanoparticle-based drug delivery systems (DDSs) have been playing a considerable role in the eradication of cancer. In this experimental study, we designed and synthesized folic acid (FA)-decorated chitosan (CS) nanocarrier for targeted delivery of miR-126 (as a therapeutic agent) to lung cancer A549 cells. Materials and methods: Therefore, the FA-CS-miR-126 nano-complex was perfectly developed and characterized by various analytical devices such as Fourier transform infrared (FT-IR) and dynamic light scattering (DLS) spectroscopies and as well as transmission electron microscopy (TEM). The size was determined lower than 100 nm for synthetics. Then, a gel retardation assay was performed to investigate the entrapment efficiency of nano-complex. Afterward, the sort of in vitro assays was implemented on A549 (FA receptor-positive lung cancer cell line) and MRC5 (normal human diploid cell line) to evaluate the therapeutic efficiency of FA-CS-miR-126. Results: As the cell viability (40.7 ± 2.98% cell viability after 72 h treatment with 500 nM), migration assay (weaker migration after 24 h and 48 h), apoptotic and autophagy genes expression level (Caspse9: sixfolds; BAX: 17 folds; ATG5: fourfolds; and BECLIN1: threefolds more than the control group), the reduced expression level of EGF-L7, as a target gene for miR-126 was evaluated by Real-Time PCR too, then, cell cycle arrest (8.66% of cells in sub-G1 phase), and cell apoptosis assay (21.0% of cancer cell in late apoptosis phase) were scrutinized. Conclusion: These results are remarkably approved the biocompatible and efficient performance of FA-CS-miR-126 as a promising DDS. Graphical Abstract","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"21 1","pages":"168 - 188"},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88594984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antheraea pernyi silk fibroin-coated adenovirus as a VEGF165-Ang-1 dual gene delivery vector","authors":"Linling Huang, Yi Jiang, Xueping Liu, Ying Guo, Yanfei Feng, Peng Pan, Mingzhong Li, Yu Liu","doi":"10.1177/08839115221095254","DOIUrl":"https://doi.org/10.1177/08839115221095254","url":null,"abstract":"Vascularization is a key challenge in the regeneration of tissues containing blood vessels. In this study, spermine was used for cationic modification of Antheraea pernyi silk fibroin (ASF) to synthesize cationized ASF (CASF). CASF/Ad complexes prepared by coating adenovirus (Ad) with CASF were used as delivery vectors for vascular endothelial growth factor 165 and angiopoietin-1 dual genes. The results showed that the zeta potential of the Ad was reversed from −7.75 mV to approximately +8.40 mV after CASF coating, and the sizes of the CASF/Ad complexes were 200 to 290 nm. Furthermore, human umbilical vein endothelial cells HUVECs were cocultured and infected with CASF/Ad in vitro. The results of confocal laser scanning microscopy, flow cytometry and CCK-8 assay showed that coating Ad with CASF at concentration of 20 and 50 µg/mL not only reduced the cytotoxicity of naked Ad, but also significantly promoted cell proliferation. Therefore, the CASF/Ad complexes could be beneficial to reduce the dosage of Ad and the potential toxicity risk of high doses of Ad in vivo, which has the potential of application to promote vascular network regeneration.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"17 1","pages":"189 - 204"},"PeriodicalIF":1.7,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82064438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methods for crosslinking and stabilization of chitosan structures for potential medical applications","authors":"A. Woźniak, M. Biernat","doi":"10.1177/08839115221085738","DOIUrl":"https://doi.org/10.1177/08839115221085738","url":null,"abstract":"Chitosan is a well-known polymer widely used in tissue engineering and regenerative medicine. It is biocompatible, biodegradable, non-toxic, has antibacterial and osteoconductive properties. Chitosan is often used in the form of composites (with the participation of ceramic particles), membranes, hydrogels or nanoparticles. The problem with biomaterials is their low durability, rapid degradation, poor mechanical properties and cytotoxicity. Cross-linking or stabilization of such materials allows for solving these problems. It is important that the compounds used for this purpose exhibit limited or no toxicity. The presented article is a review and presents some methods of cross-linking/stabilization of chitosan structures. The analysis concerns low or non-cytotoxic cross-linking/stabilization methods. The discussed compounds used for the purpose of chitosan structure fixation are: cinnamaldehyde, genipin, L-aspartic acid, vanillin, sodium carbonate, sodium alginate, BGP, ethanol and TPP. There is discussed also a hydrothermal/dehydrothermal method which seems to be promising as it is more advantageous since no additional compounds are introduced into the structure.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"36 1","pages":"151 - 167"},"PeriodicalIF":1.7,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83002480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral nerve regeneration by thiolated chitosan hydrogel containing Taurine: In vitro and in vivo study","authors":"Arian Ehterami, Nariman Rezaei kolarijani, Simin Nazarnezhad, M. Alizadeh, Alireza Masoudi, M. Salehi","doi":"10.1177/08839115221085736","DOIUrl":"https://doi.org/10.1177/08839115221085736","url":null,"abstract":"About 2.8% of trauma sick persons hurt from peripheral nerve damages, thus, numerous approaches are using to improve peripheral nerve regeneration. In the current study, the efficacy of several dosages of Taurine for peripheral nerve regeneration was evaluated. About 0.1%, 1%, and 10% (w/w) of Taurine were added into thiolated chitosan hydrogel and its features including morphology, swelling properties, weight loss, hemo-, and cytocompatibility were assessed. Hydrogels’ functionality was evaluated by injecting them into the crushed sciatic nerve of rats by using walking-foot-print analysis, Hot plate latency test, gastrocnemius muscle wet weight loss, and histopathological evaluation. Results demonstrated that the average pore size is in the area of 30–40 μm with interconnected pores and their weight loss was around 70% after 7 days. Results of blood compatibility and the MTT tests confirmed the biocompatibility of hydrogels. In vivo study illustrate thiolated Chitosan/Taurine hydrogels especially hydrogel includes 1% of Taurine enhanced sciatic nerve regeneration. In conclusion, Taurine can be used as a feasible treatment for peripheral nerve regeneration.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"79 3 1","pages":"85 - 97"},"PeriodicalIF":1.7,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90931913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}