Antheraea pernyi silk fibroin-coated adenovirus as a VEGF165-Ang-1 dual gene delivery vector

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Bioactive and Compatible Polymers Pub Date : 2022-05-01 DOI:10.1177/08839115221095254

Linling Huang, Yi Jiang, Xueping Liu, Ying Guo, Yanfei Feng, Peng Pan, Mingzhong Li, Yu Liu

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引用次数: 1

Abstract

Vascularization is a key challenge in the regeneration of tissues containing blood vessels. In this study, spermine was used for cationic modification of Antheraea pernyi silk fibroin (ASF) to synthesize cationized ASF (CASF). CASF/Ad complexes prepared by coating adenovirus (Ad) with CASF were used as delivery vectors for vascular endothelial growth factor 165 and angiopoietin-1 dual genes. The results showed that the zeta potential of the Ad was reversed from −7.75 mV to approximately +8.40 mV after CASF coating, and the sizes of the CASF/Ad complexes were 200 to 290 nm. Furthermore, human umbilical vein endothelial cells HUVECs were cocultured and infected with CASF/Ad in vitro. The results of confocal laser scanning microscopy, flow cytometry and CCK-8 assay showed that coating Ad with CASF at concentration of 20 and 50 µg/mL not only reduced the cytotoxicity of naked Ad, but also significantly promoted cell proliferation. Therefore, the CASF/Ad complexes could be beneficial to reduce the dosage of Ad and the potential toxicity risk of high doses of Ad in vivo, which has the potential of application to promote vascular network regeneration.

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柞蚕丝素包被腺病毒作为vegf165 - ang1双基因传递载体的研究

血管化是含血管组织再生的关键挑战。本研究利用精胺对柞蚕丝素(ASF)进行阳离子修饰，合成了阳离子化的柞蚕丝素(CASF)。用CASF包被腺病毒(Ad)制备CASF/Ad复合物，作为血管内皮生长因子165和血管生成素-1双基因的传递载体。结果表明，CASF包覆后，Ad的zeta电位由- 7.75 mV转变为+8.40 mV, CASF/Ad配合物的尺寸为200 ~ 290 nm。此外，将人脐静脉内皮细胞HUVECs与体外CASF/Ad共培养并感染。激光共聚焦扫描显微镜、流式细胞术和CCK-8检测结果显示，20和50µg/mL浓度的CASF包被Ad不仅降低了裸Ad的细胞毒性，而且显著促进了细胞的增殖。因此，CASF/Ad复合物在体内可能有利于降低Ad的剂量和高剂量Ad的潜在毒性风险，具有促进血管网络再生的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Bioactive and Compatible Polymers 工程技术-材料科学：生物材料

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The use and importance of biomedical polymers, especially in pharmacology, is growing rapidly. The Journal of Bioactive and Compatible Polymers is a fully peer-reviewed scholarly journal that provides biomedical polymer scientists and researchers with new information on important advances in this field. Examples of specific areas of interest to the journal include: polymeric drugs and drug design; polymeric functionalization and structures related to biological activity or compatibility; natural polymer modification to achieve specific biological activity or compatibility; enzyme modelling by polymers; membranes for biological use; liposome stabilization and cell modeling. This journal is a member of the Committee on Publication Ethics (COPE).