Journal of Applied Entomology最新文献

{"title":"Silicon Supplementation Reinforces Maize Defence to Defeat the Oriental Armyworm","authors":"Farhana Shameen,&nbsp;Abid Hussain Wani,&nbsp;Iqra Gulzar,&nbsp;Tariq Ahmad,&nbsp;Irfan Rashid","doi":"10.1111/jen.13434","DOIUrl":"https://doi.org/10.1111/jen.13434","url":null,"abstract":"<div>\u0000 \u0000 <p>Maize is a critical component of global food security; however, pests like the oriental armyworm (<i>Mythimna separata</i> Walker) cause significant yield losses to this crop. Conventional control approaches for managing this insect rely on pesticide applications, which pose environmental risks and can lead to pest resistance. Thus, the necessity for alternative management strategies is emphasised. Silicon (Si) is proposed as a pesticide substitute due to its ability to mitigate a plethora of biotic and abiotic stresses in plants. To evaluate the effectiveness of Si in controlling the armyworm, maize plants were treated with three Si concentrations (T1: 500 mg/L, T2: 750 mg/L, T3: 1000 mg/L) and then infested with armyworm larvae. The performance of the insect pest was assessed by evaluating its larval feeding and oviposition preference, mandibular morphology, survival rates and weight gain. Results showed a mean larval survival rate of 63% in T3 plants compared to 96% in control. The weight of larvae fed on control leaves was twice that of those fed on Si-treated leaves. Likewise, the incisor length of larvae fed on Si-treated leaves was significantly shorter (177 μm) than those fed on control leaves (447 μm). Moreover, Si-treated leaves were less favourable for egg laying, with only 20% of eggs laid on them. These findings indicate that Si supplementation enhances maize resistance to the infestation of the oriental armyworm. This Si-mediated resistance of maize plants against the oriental armyworm underscores the potential of Si supplementation as a sustainable alternative to chemical pesticides in integrated pest management strategies.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 7","pages":"1059-1069"},"PeriodicalIF":1.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sublethal Effects of Voliam Flexi on Spodoptera frugiperda (Lepidoptera: Noctuidae): Insights From Life Table Theory","authors":"Gulfam Yousaf,&nbsp;Muhammad Arshad,&nbsp;Muhammad Irfan Ullah,&nbsp;Muhammad Zeeshan,&nbsp;Hamza Latif,&nbsp;Muhammad Mudassar Sharif,&nbsp;Wang Chunjuan,&nbsp;Lamya Ahmed Alkeridis,&nbsp;Desong Yang","doi":"10.1111/jen.13431","DOIUrl":"https://doi.org/10.1111/jen.13431","url":null,"abstract":"<div>\u0000 \u0000 <p>Fall armyworm, <i>Spodoptera frugiperda</i> (J.E. Smith) (Lepidoptera: Noctuidae), is a major insect pest that is responsible for huge economic losses to maize crops. The study on the sublethal effect of insecticide on <i>S. frugiperda</i> is crucial for its comprehensive management in the field. Thus, the current study evaluated the sublethal effects of Voliam Flexi, which is a combination of thiamethoxam and chlorantraniliprole, on the demographic parameters of <i>S. frugiperda</i> exposed to LC₁₀, LC₂₀ and LC₃₀ concentrations. The results indicated that <i>S. frugiperda</i> larvae showed a longer period (35.4 days at LC₁₀, 37.9 days at LC₂₀ and 39.5 days at LC₃₀) compared to those in the control group (30.4 days). The fecundity rate was also reduced when sublethal concentrations were applied (298.1 eggs at LC₁₀, 253.2 eggs at LC₂₀ and 214.1 eggs at LC₃₀) compared to the control group (339.3 eggs). The larvae exposed to sublethal doses exhibited a significant decrease in the intrinsic rate of increase (<i>r</i>), net reproduction rate (<i>R</i>₀) and finite rate of increase (<i>λ</i>) compared to the control group. These findings suggest that sublethal concentrations of Voliam Flexi adversely affect the growth and reproductive capacity of <i>S. frugiperda</i>, ultimately aiming to keep populations below levels that cause economic damage. Our findings highlight the potential of sublethal concentrations of Voliam Flexi for the effective implementation of the IPM plan in the field against <i>S. frugiperda</i>.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 7","pages":"1040-1049"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating Required Sample Sizes for Gut Content Metabarcoding Studies of Dietary Diversity in Spiders","authors":"Anja Carina Melcher,&nbsp;Henrik Krehenwinkel,&nbsp;Danilo Harms,&nbsp;Klaus Birkhofer","doi":"10.1111/jen.13433","DOIUrl":"https://doi.org/10.1111/jen.13433","url":null,"abstract":"<p>Spiders are dominant predators in terrestrial ecosystems, regulating invertebrate biomass, diversity and abundance through predation. However, analysing predation patterns in the field is challenging, especially as spiders are extra-intestinal liquid feeders and often active at night. Gut content metabarcoding (GCM) provides a cost-effective method to study the prey composition of spiders. In this study, we determine the minimum sample size of spider specimens required to obtain representative estimates of local prey composition. Six common spider species were studied using GCM, and we show that the number of analysed individuals per spider species influenced the sampling completeness for prey at different taxonomic levels. To achieve 90% sampling completeness at the prey species level, between 50 and over 150 individuals per spider species had to be analysed, which is challenging for ecological multi-site studies. Hence, we argue for consideration of coarser taxonomic levels, such as family or order, which often provide sufficient ecological information to understand predator–prey interactions. At these levels, sampling completeness can be achieved with much smaller sample sizes of four to eight individuals per species, making ecological GCM studies more cost-effective. These results highlight the benefits of considering the taxonomic resolution in metabarcoding studies to address ecological research questions. We further provide information that will facilitate future investigations of predator–prey dynamics, not just in spiders but also in other extra-intestinal liquid feeders like, for example, ground beetles.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 7","pages":"1034-1039"},"PeriodicalIF":1.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate Change Impacts on Diapause Outcomes in Bombus terrestris Across an Environmental Gradient","authors":"Penelope R. Whitehorn,&nbsp;Romy Rehschuh,&nbsp;Stephanie Rehschuh,&nbsp;Scott A. L. Hayward,&nbsp;Calum Brown","doi":"10.1111/jen.13432","DOIUrl":"https://doi.org/10.1111/jen.13432","url":null,"abstract":"<p>Most temperate insects survive winter by entering a period of dormancy called diapause. Some of the greatest climate change impacts are likely to occur during this period, as entire populations of some species are represented by a relatively small number of hibernating individuals. Bumblebee populations are particularly vulnerable because only queens overwinter and any decline in the winter survival of queens, or reduced post-diapause fitness, could have significant consequences on population dynamics the following spring. This study investigated the impact of different overwintering conditions on <i>Bombus terrestris</i> queens across an altitudinal gradient in the German Alps. Snow pack coverage was manipulated to simulate advanced snow melt and delayed snow melt, and maintained for current/control conditions. We found that changes in snow pack and altitude affect the level and variability of soil temperature across the winter season but that these have no effect on hibernation survival (very high in all cases) or subsequent colony establishment (very low in all cases). However, queen weight loss during diapause did differ across altitudes, with bumblebees hibernating at lower levels and being exposed to higher and more widely fluctuating temperatures, losing significantly more weight. Our results therefore confirm the potential for negative physiological impacts of exposure to higher and more variable winter temperatures during diapause, as is increasingly common under climate change. But we also find that bumblebees have some resilience to these effects, at least in terms of hibernation survival, within the range experienced in our experiments. We conclude that further field studies targeted at the range of conditions likely to be experienced by European insects over winter are necessary to anticipate potential population impacts, but that existing levels of climate change are likely sufficient to affect many of these insects, even if at sub-lethal levels.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 7","pages":"1023-1033"},"PeriodicalIF":1.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Escape Behaviour of Dalbulus maidis (DeLong and Wolcott): Subsidies for Insecticide Application and Field Sampling","authors":"Charles Martins de Oliveira,&nbsp;Ranyse Barbosa Querino,&nbsp;Erika Aylanna Gomes de Jesus,&nbsp;Mateus de Souza Sanches,&nbsp;Michely Ferreira Santos de Aquino,&nbsp;Hebert Ribeiro de Souza,&nbsp;Marisa Lisboa de Brito,&nbsp;Juaci Vitória Malaquias,&nbsp;Giovanna Ferreira da Conceição,&nbsp;Marina Regina Frizzas","doi":"10.1111/jen.13429","DOIUrl":"https://doi.org/10.1111/jen.13429","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Dalbulus maidis</i> (Hemiptera: Cicadellidae) is the primary pest of corn crops in Brazil due to its high efficiency in transmitting pathogens associated with the corn stunt disease complex, including maize bushy stunt phytoplasma (MBSP) and corn stunt spiroplasma (CSS), as well as maize rayado fino virus (MRFV) and maize striate mosaic virus (MSMV). However, many aspects of the behaviour and bioecology of this insect vector are still poorly understood. Accordingly, we investigated the escape behaviour of <i>D. maidis</i> at three different times of the day (6:00 a.m., 1:30 and 6:00 p.m.) under controlled conditions. This behaviour was compared between sexes and among populations of healthy leafhoppers and those carrying bacteria that cause corn stunt diseases (phytoplasma and spiroplasma). The studies were conducted in November/2022 (first crop season conditions) and April/2023 (second crop season conditions). Groups of 100 leafhoppers (50 males and 50 females), healthy or infected with phytoplasma or spiroplasma, were placed in cages containing maize plants. Following the application of mechanical disturbances, the number of individuals that left the maize plants was recorded. Temperature positively influenced the escape behaviour of <i>D. maidis</i>. Moreover, a higher proportion of leafhoppers abandoned maize plants under conditions that simulated the first crop season (27.3%) than the second crop season (14.3%). This propensity was consistently higher during the hottest period of the day (1:30 p.m.). Meanwhile, infected leafhoppers were less active than healthy leafhoppers under the second crop season conditions and lower average temperatures. No differences were observed in escape behaviour between the sexes. The behaviour of <i>D. maidis</i> observed in this study supports the recommendation of applying insecticides during the cooler hours of the day, which may enhance their effectiveness against this insect vector. Similarly, field sampling involving direct insect counts on maize plants is also recommended under these same conditions.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 8","pages":"1147-1156"},"PeriodicalIF":2.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Scale Factors Promote Entomophilous Pollination: Productive Blueberry Agroecosystems as a Study Model","authors":"P. Cavigliasso,&nbsp;N. Maza,&nbsp;C. G. Barreto,&nbsp;M. E. Maina,&nbsp;G. P. Gennari,&nbsp;N. P. Chacoff","doi":"10.1111/jen.13428","DOIUrl":"https://doi.org/10.1111/jen.13428","url":null,"abstract":"<div>\u0000 \u0000 <p>Blueberry cultivation requires insect-mediated pollination for optimal fruit formation. Studies have shown that improving the efficiency of pollen exchange during flowering increases both the quantity and quality of fruit. Blueberry flowers have a unique anther structure that facilitates pollen release through a specialised interaction called ‘buzz pollination’, which is performed by wild bee species rather than <i>Apis mellifera</i>. The study aims to assess the effects of pollination management, weed management, and landscape heterogeneity on pollinator communities, and their interaction with the crop and blueberry fruit set. Nine blueberry plots were studied to investigate the influence of including <i>Bombus pauloensis</i> hives, the presence of inter-row vegetation, and landscape heterogeneity on pollinator abundance, richness, visitation rate, and fruit production. Generalised linear mixed models (GLMM) were used to analyse the relationship between these factors. No significant differences in fruit formation were found between plots receiving pollination services from <i>Apis mellifera</i> and those with a combination of <i>A. mellifera</i> + <i>B. pauloensis</i> hives. However, inter-row vegetation had a positive effect on pollinator abundance and visitation rate to blueberry flowers. The increase in cultivated areas had a negative effect on the visitation rate of both managed and native pollinators. Conversely, more diverse landscapes had a negative and significant effect on <i>A. mellifera</i> abundance within the plots. These findings suggest the importance of implementing wild pollinator-friendly management practices, carefully assessing honeybee densities, and managing resources at both local and landscape scales to enhance bee visitation and blueberry fruit production.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 6","pages":"1010-1022"},"PeriodicalIF":1.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollen Transport Networks Help Reveal the Importance of Dipteran Pollinators in Coastal Plant Communities","authors":"Alexander Suárez-Mariño,&nbsp;Gerardo Arceo-Gómez,&nbsp;Cristopher Albor,&nbsp;Victor Parra-Tabla","doi":"10.1111/jen.13426","DOIUrl":"https://doi.org/10.1111/jen.13426","url":null,"abstract":"<div>\u0000 \u0000 <p>The contribution of dipterans to pollination in tropical communities has been scarcely studied. Although some studies have evaluated dipteran pollination in crops and wild species, limited attention has been paid to their role in pollination networks. In particular, their role in pollen transport has been neglected. Integrating their role as floral visitors and their importance in pollen transport through interaction networks may help reveal their importance in pollination in tropical co-flowering communities. We constructed visitation and pollen transport networks from the analysis of pollen carried on dipteran bodies in two tropical coastal communities to test the prediction that pollen transport networks would reveal fewer but more specialised interactions than visitation networks. We estimated parameters describing the network topology and the main estimators of the roles of each species. We recorded 1372 floral visits and counted 46,001 pollen grains. Our results showed that the visitation and pollen transport networks exhibited similar pollination network structures (e.g., nestedness, specialisation, and modularity). However, the pollen transport network suggested a higher level of specialisation between dipterans and plants in both communities. In addition, the networks' modular structure suggested contrasting species grouping according to network type. Moreover, most dipterans were classified as peripheral species in pollen transport networks, suggesting high within-module interactions. Although some estimators of dipteran role through visitation (nestedness contribution and resource range) can be used as indicators of pollen transport, we found that pollen transport is a better descriptor of dipteran specialisation or the role of the species in the modular structure of the network. This work is the first to explore the importance of dipterans using visitation and pollen transport data in the tropics. Overall, our results suggest that a better understanding of the dipteran's importance in pollination networks is necessary to add information on their role in pollen transport.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 6","pages":"996-1009"},"PeriodicalIF":1.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Barcoding Reveals Taxonomic Insights Into Pest Species of Leafhoppers (Hemiptera: Cicadellidae) on Fruit Trees in South Korea","authors":"Hwalran Choi,&nbsp;Sun-Kook Kim,&nbsp;Duane D. McKenna,&nbsp;Seunghwan Lee","doi":"10.1111/jen.13402","DOIUrl":"https://doi.org/10.1111/jen.13402","url":null,"abstract":"<div>\u0000 \u0000 <p>Severe leafhopper pests infesting fruit trees (apricot, grape, peach, persimmon and plum) in South Korea were investigated. DNA barcoding using mitochondrial cytochrome c oxidase (COI) sequences identified four Typhlocybinae species: <i>Arboridia kakogawana</i> and <i>Arboridia maculifrons</i> on grape, <i>Singapora shinshana</i> on <i>Prunus</i> trees and <i>Zorka</i> sp. on persimmon, spanning both nymphal and adult life stages. Notably, DNA barcoding revealed phylogenetically relevant colour variation among adult specimens of the grape pest, <i>A. kakogawana</i>. This study presents, for the first time, live nymphal and adult photographs alongside neighbour-joining trees, host plant associations and updated DNA barcode sequences for these Korean leafhoppers, which have not previously been included in global datasets.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 6","pages":"988-995"},"PeriodicalIF":1.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pest Control Strategies on Arthropod Pests of Apple in Europe: A Meta-Analysis","authors":"Ingrid Aline Bapfubusa Niyibizi,&nbsp;Pia Addison,&nbsp;Klaus Birkhofer","doi":"10.1111/jen.13416","DOIUrl":"https://doi.org/10.1111/jen.13416","url":null,"abstract":"<p>Apple (<i>Malus domestica</i> Borkh.) is a major fruit crop in Europe. More than 200 phytophagous arthropod species thrive in European apple orchards, several of which are economically important pests. Due to the pest pressure on apple production, consumer demand for residue-free products and current policies to make food production in the European Union more sustainable, pest management has become an important issue in meeting the high cosmetic standards expected for apples on the fresh fruit market. The aim of this study was to determine the most effective management strategy to reduce the abundance of arthropod pests of apples in Europe. We conducted a Europe-wide meta-analysis to evaluate the effectiveness of integrated pest management (IPM) and organic management in reducing the abundance of arthropod pests. We also examined the differences between synthetic and organic pesticides in terms of pest reduction as a core element of the difference between IPM and organic apple production systems in Europe. We found an overall nonsignificant effect of IPM compared to organic management. However, when comparing synthetic pesticides with organic pesticides, we found that synthetic pesticides were significantly more effective. On the other hand, organic pesticides showed a significant positive effect in controlling pest populations when compared to an absence of treatment. This analysis highlights the effectiveness of synthetic pesticides over organic pesticides, while accounting for the inherent heterogeneity within each group, and suggests that factors beyond insecticide use play an important role in pest reduction in organic and IPM apple production systems in Europe.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 6","pages":"957-987"},"PeriodicalIF":1.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13416","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144255959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Oscheius colombianus (Nematoda: Rhabditidae) in the Philippines and Its Virulence Against Different Developmental Stages of the Common Cutworm, Spodoptera litura","authors":"Mayvel D. Suan,&nbsp;Prakaijan Nimkingrat,&nbsp;Sitaram Aryal,&nbsp;Aashaq Hussain Bhat,&nbsp;Atirach Noosidum,&nbsp;Farid S. Ataya,&nbsp;Nanette Hope Sumaya","doi":"10.1111/jen.13418","DOIUrl":"https://doi.org/10.1111/jen.13418","url":null,"abstract":"<div>\u0000 \u0000 <p>Entomopathogenic nematodes (EPNs) of the genera <i>Heterorhabditis</i> and <i>Steinernema</i> are widely used as biological control agents (BCAs) against agricultural insect pests. Recently, nematodes from the genus <i>Oscheius</i> have shown potential as candidates for biological control agents. In this study, two nematode populations from the agricultural fields in Bukidnon, Philippines, were isolated using the traditional insect soil baiting technique with superworm (<i>Zophobas morio</i>). Through morphological, morphometrical, and molecular analyses, these nematodes, labelled as BRT2 and CSS4, were identified as <i>Oscheius colombianus</i>, the country's first record of the species. Preliminary laboratory assays were conducted to evaluate the efficacy of the isolated nematodes against <i>Z. morio</i> larvae. The results showed that the isolate BRT2 exhibited higher efficacy than the isolate CSS4. Isolate BRT2 was tested against different developmental stages of <i>Spodoptera litura</i> and effectively controlled all stages. The fourth and fifth instar larvae showed the highest susceptibility within 72–96 h post-inoculation at various test concentrations (500, 300 and 100 IJs/larvae). However, 2-day-old pupae had the lowest mortality rate among all treatments, with the commercially available <i>Steinernema siamkayai</i> Thai strain (SST1) serving as the standard control. This study highlights the potential of locally isolated nematodes as biocontrol agents for combating different stages of <i>S. litura</i>. The findings contribute to the growing research on EPNs and their applications in agricultural pest management.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 5","pages":"795-810"},"PeriodicalIF":1.7,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143939141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}