Journal of Applied Entomology最新文献

{"title":"Interactions Between Woolly Whiteflies and Their Parasitoid Wasp: Development, Competition, and Reproductive Potential","authors":"Gila Hanuka,&nbsp;Inon Scharf","doi":"10.1111/jen.13401","DOIUrl":"https://doi.org/10.1111/jen.13401","url":null,"abstract":"<div>\u0000 \u0000 <p>Whiteflies are important pests that severely impact crop yield through feeding and as vectors of pathogens. We focus on the woolly whitefly, <i>Aleurothrixus floccosus</i>, and its parasitoid wasp, <i>Cales noacki</i>, used for biological control. This research is motivated by the challenges of managing whitefly populations, intensified by the limitations of pesticides and the increasing prevalence of whitefly infestations in new regions worldwide. Our findings reveal that parasitism by <i>Cales noacki</i> prolongs the development time of woolly whiteflies when the whitefly eventually overcomes the parasitoid. Wasps emerging from the fourth instar of the whitefly nymphs were larger and had higher reproductive potential than those emerging from earlier instars. We found a positive correlation between wasp size and the number of eggs in the ovary for wasps emerging from the third instar of the whitefly nymphs. However, no such correlation was found for wasps emerging from the fourth instar. Additionally, competition between whiteflies and other common pests, such as citrus mealybugs and citrus mites, seemed to hinder the emergence success of whiteflies, suggesting that multi-pest interactions could affect biological control strategies. These results demonstrate the complex life-history interactions between woolly whiteflies and their parasitoid wasps, pointing to the importance of integrating ecological considerations into pest management practices. Understanding these dynamics is important for developing effective control methods, especially in the face of global climate change, which might further reduce the efficiency of parasitoid wasps.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 4","pages":"549-557"},"PeriodicalIF":1.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Research Hotspots and Frontiers of Bumblebees During 1999–2024: A Bibliometric Analysis","authors":"Yige Yuan,&nbsp;Guy Smagghe,&nbsp;Xiangsheng Chen,&nbsp;Jiankun Long,&nbsp;Zhimin Chang","doi":"10.1111/jen.13400","DOIUrl":"https://doi.org/10.1111/jen.13400","url":null,"abstract":"<div>\u0000 \u0000 <p>Bumblebees, as social species and efficient pollinators, have attracted the attention of researchers and governments all over the world. However, a comprehensive overview of the bumblebee research field is lacking. This study delves into a bibliometric analysis of the recent 25 years of research with bumblebees from 1999 to 2024. Drawing data from 4119 publications in the Web of Science (WoS) database, it showed a steady increase in number of publications in the recent two decades. Countries in North America, Europe and China formed the three main forces. The hotspots and frontiers were related to decline and health of bumblebees, including combined effects related to climate change, agrochemicals, habitat loss and fragmentation, parasites and disease, and monotonous diets and competition. Additionally, based on co-cited references cluster analysis, it was concluded that climate change and pesticides will still be the focus research fields in the future. Overall, this paper serves as an exhaustive resource for individuals and governments working intrigued by bumblebee.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 4","pages":"536-548"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osmia cornuta Is a More Suitable Managed Pollinator for Cherry and Apple Orchards Than Osmia bicornis","authors":"Laurie Magnin,&nbsp;Felix Bianchi,&nbsp;Steffen Hagenbucher","doi":"10.1111/jen.13399","DOIUrl":"https://doi.org/10.1111/jen.13399","url":null,"abstract":"<p>Managed crop pollination strongly relies on the honeybee <i>Apis mellifera</i> and the bumblebee <i>Bombus terrestris</i>, which is risky, may impact wild pollinator communities and does not always give the best pollination outcomes. The mason bees <i>Osmia cornuta</i> and <i>Osmia bicornis</i> are increasingly used as alternative crop pollinators, but it is not clear how suitable these species are for fruit pollination. Here we assessed the suitability of <i>O. cornuta</i> and <i>O. bicornis</i> as managed pollinators in two cherry and four apple orchards. <i>O. cornuta</i> and <i>O. bicornis</i> were introduced in the orchards at the start of blooming. We assessed their time till emergence, nesting rate, abundance in orchards via transect walks, flower visitation rate in 20-min time counts and pollen composition on their bodies. <i>O. cornuta</i> emerged 4 ± 2 days earlier and had a minimum of 5-fold higher nesting rate than <i>O. bicornis</i> in cherry and apple orchards. Transect walks revealed that <i>O. cornuta</i> was more abundant in orchards than <i>O. bicornis</i> (21.2% ± 10.1% vs. 1.3% ± 1.8% of all observed pollinators) and more frequently observed visiting flowers (53 vs. 5 visits for <i>O. cornuta</i> and <i>O. bicornis</i>, respectively). Pollen loads of <i>O. cornuta</i> contained 95% of Rosacea plants (likely apple and cherry), whereas this was 30% for <i>O. bicornis</i>, indicating that <i>O. bicornis</i> mainly foraged outside the orchards. These findings indicate that <i>O. cornuta</i> is a better pollinator than <i>O. bicornis</i> for early blooming crops, such as cherry and apple, in the relatively cool and rainy weather conditions during our study.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 4","pages":"524-535"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Organic and Synthetic Fertiliser on Insect Herbivore Populations in Quinoa","authors":"Liesl Oeller,&nbsp;Benjamin W. Lee,&nbsp;Saumik Basu,&nbsp;Kevin M. Murphy,&nbsp;David W. Crowder","doi":"10.1111/jen.13398","DOIUrl":"https://doi.org/10.1111/jen.13398","url":null,"abstract":"<p>Fertilisers are used in agriculture to promote crop productivity. There are many nutrient sources in fertilisers, and different sources cause variation in plant defence and pest tolerance. Assessing how pests respond to different types of fertilisers is key to develop integrated pest management programs. Here, we assessed how two fertilisers applied to quinoa (<i>Chenopodium quinoa</i> Willd.) affected cowpea aphid (<i>Aphis craccivora</i> Koch<i>.</i>) and <i>Lygus spp.</i> survival and reproduction. Quinoa is a newly introduced crop in the USA, and fertiliser regimes using organic or synthetic nitrogen sources are being evaluated. We quantified growth and survival of each pest on organic- and synthetic-fertilised quinoa for two varieties in cages and the open field. We also quantified gene expression associated with three phytohormones involved in chemical defence to identify potential mechanisms of treatment effects. In cages, organic-fertilised quinoa had the fewest aphids and lowest <i>Lygus</i> survival. However, the effects were weaker in the open field, where aphids were marginally less abundant on organic-fertilised plants and no effects of fertiliser on <i>Lygus</i> were observed. There was also evidence for fertiliser altering defence gene expression, suggesting a possible mechanism impacting pest behaviour and fitness. Overall, our study shows that fertilisers from different sources may provide varying levels of pest control in quinoa crops.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 4","pages":"515-523"},"PeriodicalIF":1.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pairing Residual Contact Insecticide Use With Long-Lasting Insecticide-Incorporated Netting to Reduce Dispersal and Damage by Stored Product Insects","authors":"Sabita Ranabhat,&nbsp;Jennifer Abshire,&nbsp;Joseph Castaldi,&nbsp;Deanna S. Scheff,&nbsp;Georgina V. Bingham,&nbsp;Tanja McKay,&nbsp;Kun Yan Zhu,&nbsp;William R. Morrison III","doi":"10.1111/jen.13396","DOIUrl":"https://doi.org/10.1111/jen.13396","url":null,"abstract":"<p>Long-lasting insecticide-incorporated netting (LLIN) can intercept insects and has shown promise for pest management before and after harvest in agriculture. Understanding how to incorporate LLIN into existing integrated pest management (IPM) programs, with other commonly used tactics like residual contact insecticides, can significantly enhance IPM programs at food facilities. In this study, we assessed whether LLIN (0.34% alpha-cypermethrin, BASF) may enhance the effects of residual contact insecticides such as Centanyl EC (Central Life Sciences), active ingredient (a.i.) deltamethrin and Evergreen (McLaughlin Gormley King Co.), a.i. natural pyrethrins against <i>Tribolium castaneum</i> and <i>Rhyzopertha dominica</i>. We recorded their dispersal and progeny production in treatments with LLIN alone, deltamethrin or pyrethrins alone, LLIN + insecticide, or no treatment (positive control). We demonstrated the use of both LLIN and residual insecticide with either deltamethrin or pyrethrins reduced adult insect dispersal to the food sources compared to the control, but only increased control of progeny production marginally by 8%–19%. LLIN alone was highly effective in reducing progeny production of <i>T. castaneum</i> by 40% compared to the positive control. Importantly, pyrethrins did not cause significant direct mortality or prevent progeny production when used alone against <i>T. castaneum</i>. However, the combined use of pyrethrins with LLIN showed an enhanced efficacy against the insect. Together, the combined use of LLIN and the residual contact insecticides evaluated in our study may have some benefits over using residual contact insecticide alone to manage stored product insects.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 4","pages":"501-514"},"PeriodicalIF":1.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13396","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-Driven Variations in Fitness and Physiological Responses of Aphis craccivora Koch and Aphis gossypii Glover (Hemiptera: Aphididae) on Lablab purpureus subsp. Bengalensis (Jacq.) Verdc","authors":"Sanoj Kumbhakar,&nbsp;Susmita Das,&nbsp;Bhramar Bhattacharyya,&nbsp;Anandamay Barik","doi":"10.1111/jen.13394","DOIUrl":"https://doi.org/10.1111/jen.13394","url":null,"abstract":"<div>\u0000 \u0000 <p>Temperature, an important abiotic factor, alters physiological processes in insects. This study includes effect of temperatures under laboratory (7°C, 12°C, 17°C, 22°C, 27°C and 32°C) and field (10°C–26°C) conditions on the life table parameters, total reactive oxygen species (ROS), hydrogen peroxide (H₂O₂), malondialdehyde (MDA) content and activities of antioxidative [superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX)], detoxifying [acetylcholinesterase (AChE), β-glucosidase and glutathione-S-transferase (GST)] and oxidoreductive [peroxidase (POD) and polyphenol oxidase (PPO)] enzymes of <i>Aphis craccivora</i> Koch and <i>A. gossypii</i> Glover (Hemiptera: Aphididae) on <i>Lablab purpureus</i> subsp. <i>bengalensis</i> (Jacq.) Verdc. plants. The longevity and fecundity of both aphids were highest at 22°C, and <i>A. craccivora</i> showed similarity between 22°C and field condition but <i>A. gossypii</i> showed similarity between 17°C and field condition, suggesting that <i>A. craccivora</i> is able to withstand more fluctuating temperature stress than <i>A. gossypii</i>. Total ROS, H₂O₂ and MDA content were increased at low (7°C) or high (32°C) temperature but these parameters were more increased at high temperature than low temperature, suggesting that high-temperature stress is more harmful for both aphids. Under low (7°C) or high (32°C) temperature stress, the levels of antioxidative, detoxifying and oxidoreductive enzymes were increased in both adult aphids compared to 22°C or field condition, implicating that both these aphids are trying to detoxify excess ROS by producing more antioxidative, detoxifying and oxidoreductive enzymes. The mortality of both aphids was higher at low (7°C) and high (32°C) temperatures, implicating that both aphids could not detoxify excess ROS by the coordinated action of antioxidative, detoxifying and oxidoreductive enzymes. The findings of this study can assist to envisage the population dynamics of these two important aphids, which can assist in management of these two sucking insect pests in the crop field.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 4","pages":"478-500"},"PeriodicalIF":1.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing Pest Risk in Birch Wood Products—The Effective Heat Treatment for Bronze Birch Borer Agrilus anxius (Coleoptera: Buprestidae) Prepupae","authors":"Meghan K. Noseworthy,&nbsp;Eric A. Allen,&nbsp;Christian Hébert,&nbsp;Sébastien Bélanger,&nbsp;Esme P. John,&nbsp;Christian J. K. MacQuarrie,&nbsp;Véronique Martel,&nbsp;Tyranna J. Souque","doi":"10.1111/jen.13390","DOIUrl":"https://doi.org/10.1111/jen.13390","url":null,"abstract":"<p>The protection of forest resources and the safe trade of forest products require phytosanitary measures to reduce the risk of pest movement to novel environments. Heat treatment is a widely available, efficient and effective method to produce phytosanitary wood products destined for trade. Defining the minimum effective heat treatment dose required to cause the mortality of insects in wood products reduces the risk of spreading pests to novel environments with the lowest possible energy cost, which in turn reduces environmental impacts and provides confidence in current guidelines for heat treatment regulations. The minimum effective heat treatment dose (time and temperature) for <i>Agrilus anxius</i> (bronze birch borer) prepupae was determined using the Humble water bath applying heat in vitro. Heat treatment was assessed using a controlled increase in temperature to simulate the heat ramp applied to wood in industrial kilns and conventional heat chamber operations. Target temperatures between 51°C and 56°C for exposure durations of 15 and 30 min were tested to determine the minimum effective dose. Prepupal <i>A. anxius</i> did not survive exposure to 53°C, 54°C, 55°C or 56°C for 30 min or 54°C and 56°C for 15 min. Chronic or delayed mortality was observed at 53°C for 15-min treatments. Evaluating the effect of specific heat treatment parameters for other forest pests is recommended to identify and validate the minimum temperature and time required to cause the mortality of wood pests in order to avoid introducing non-indigenous species with wood products and limit pest movement.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 3","pages":"430-441"},"PeriodicalIF":1.7,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Legume-Colonising Aphids as Potential PNYDV-Vectors: Reproduction and Infestation Dynamics in Two Different Field Bean Varieties","authors":"Ahmed Berawe,&nbsp;Heiko Ziebell,&nbsp;Judith Seeger,&nbsp;Helmut Saucke","doi":"10.1111/jen.13391","DOIUrl":"https://doi.org/10.1111/jen.13391","url":null,"abstract":"<p>Faba bean can be infected by <i>Pea necrotic yellow dwarf virus</i> (PNYDV), a novel pathogen throughout Europe, and transmitted persistently by several legume-colonising aphid species. Recent field screenings in Austria and Germany of different faba bean varieties were encouraging as consistently the lowest viral symptom development in the variety ‘GL Sunrise’ was reported. However, our own greenhouse experiments revealed that ‘GL Sunrise’ was not resistant to PNYDV but showed a similar susceptibility such as other common varieties, for example, ‘Fuego’. Therefore, we suspected varietal host traits were affecting the reproductive potential of virus vector species with reduced virus transmission, probably explaining the empirical superior health status in the field. Under greenhouse conditions, vector-specific intrinsic rates of increase (<i>r</i>_<i>m</i>) for <i>Acyrthosiphon pisum</i> Harris <i>Aphis fabae</i> Scopoli and <i>Megoura viciae</i> Buckton were similar for both varieties, except for a particular <i>A. pisum</i>-biotype derived from field pea, with a significantly reduced <i>r</i>_<i>m</i> and fecundity in ‘GL Sunrise’. Furthermore, vector abundance assessments in multisite varietal showed <i>A. pisum</i> as the predominant species. In overall cross-year and -site evaluation, early aphid census in ‘GL Sunrise’ at growth stage BBCH 61–65 indicated significantly lower primary <i>A. pisum</i>-infestation pressure (factor 0,43 relative to ‘Fuego’). This lower infestation level in ‘GL Sunrise’ was kept until BBCH 77–79 but the difference had diminished down to the non-significant factor of 0,74 <i>A. pisum</i>-density relative to ‘Fuego’ in the late census, respectively. Based on our findings of (i) basic PNYDV susceptibility, (ii) similar reproductive vector performance of key aphid species, together with (iii) a significantly weaker initial <i>A. pisum</i>-infestation levels in the field, we hypothesise ‘GL Sunrise’-specific additional antixenosis/antibiotic traits, must influence host recognition and settling behaviour of alighting (viruliferous) migrants and/or apteres, which finally determine the superior PNYDV-health status in ‘GL Sunrise’ at field conditions.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 3","pages":"442-451"},"PeriodicalIF":1.7,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Diagnostic Approach Using qPCR, ddPCR and LAMP-Based Molecular Tools for Two Leafhopper Species Identification (Nephotettix cincticeps and Nephotettix virescens)","authors":"Minkyu Jeong,&nbsp;Murtaza Khan,&nbsp;Md-Mafizur Rahman,&nbsp;Moniruzzaman Kabir,&nbsp;Nakjung Choi,&nbsp;JaeBuhm Jeon,&nbsp;Juil Kim","doi":"10.1111/jen.13393","DOIUrl":"https://doi.org/10.1111/jen.13393","url":null,"abstract":"<div>\u0000 \u0000 <p>Accurate identification of the leafhopper species <i>Nephotettix cincticeps</i> and <i>N. virescens</i> is necessary because they feed on phloem sap and transmit plant pathogens including viruses that can cause significant damage to rice plants. Initially, we examined their physical traits using a microscope and a specific camera and observed differences between the two species. To further validate these distinctions on a molecular level, we designed primers based on mitochondrial genome sequences obtained from the NCBI database. The primers were effectively used in general PCR and loop-mediated isothermal amplification (LAMP) tests. General PCR was successfully performed at 61°C using NC_mt5117F33 and NC_mt5438B33 primers for <i>N. cincticeps</i> and NV_mt3089F31, NV_mt3360B31 primers for <i>N. virescens</i>. The effectiveness of the selected primers was validated using quantitative PCR (qPCR) on the pooled gDNA samples and droplet digital PCR (ddPCR). The best conditions for LAMP were 61°C and time 45 min, resulting in the most favourable amplification outcomes. Additionally, LAMP enables diagnosis at the individual level in the field, and qPCR and ddPCR can be used for relative or absolute quantification, even from pooled gDNA from multiple species. This study emphasises the importance of combining traditional and molecular techniques in primary pest species diagnostics to ensure precise and timely pest control, which can contribute to improved pest management strategies.</p>\u0000 </div>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 3","pages":"417-429"},"PeriodicalIF":1.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vector Biology of the Soft Scales Parthenolecanium corni (Bouché) and Parthenolecanium persicae (Fabricius) (Hemiptera: Coccidae) With Grapevine Leafroll-Associated Viruses and Grapevine Virus A","authors":"Gérard Hommay,&nbsp;Antoine Alliaume,&nbsp;Monique Beuve,&nbsp;Jean-Michel Hily,&nbsp;Etienne Herrbach","doi":"10.1111/jen.13387","DOIUrl":"https://doi.org/10.1111/jen.13387","url":null,"abstract":"<p>Controlled experiments were conducted to enrich our knowledge on the transmission of different grapevine leafroll-associated ampeloviruses (GLRaV-1, -2, -3 and -4) by <i>Parthenolecanium corni</i> scale insect and to look for vector ability of <i>Parthenolecanium persicae.</i> GLRaV-1 and the vitivirus GVA were transmitted by <i>P. corni</i> to healthy plants after acquisition on GLRaV-1 and GVA-infected grapevines (<i>Vitis vinifera</i>); however, GVA was not transmitted after acquisition either on GVA singly infected vines or <i>Nicotiana benthamiana,</i> or on purified GVA virions. These results support the hypothesis of ampelovirus assistance towards vitivirus during transmission. Transmission experiments with <i>P. corni</i> and different GLRaV-4 strains showed a single transmission of strain 5. No transmission was observed with <i>P. persicae</i> after acquisition on GLRaV-1, -3 and GVA coinfected source vines. <i>P. corni</i> L2 collected from an infected vine and placed on a nonhost plant tested positive for GLRaV-1 and -3 until at least 30 and 50 days, respectively. These results contrast with the few days retention times generally reported in mealybugs. More work is needed to determine whether virions are conserved and, if so, their location inside the insects.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"149 3","pages":"405-416"},"PeriodicalIF":1.7,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jen.13387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}