Journal of Applied Entomology最新文献

{"title":"Interannual and seasonal migratory patterns of wheat aphids across the Bohai Strait in eastern Asia","authors":"Xingya Wang, Haotian Ma, Ying Gao, Mingli Yu, Yuechao Zhao, Kongming Wu","doi":"10.1111/jen.13349","DOIUrl":"https://doi.org/10.1111/jen.13349","url":null,"abstract":"The bird cherry‐oat aphid (<jats:italic>Rhopalosiphum padi</jats:italic>) and the grain aphid (<jats:italic>Sitobion miscanthi</jats:italic>) are two destructive wheat pests that have caused severe economic losses in China's main wheat‐producing regions. Although previous studies have focused mainly on the population dynamics of wheat pests in wheat fields, little is known about the trans‐sea migration of both wheat aphid species in eastern China. Here, we conducted 10 years of monitoring from 2012 to 2021 to determine aerial migration patterns utilizing a suction trap on Beihuang Island (BH), a small island in the centre of the Bohai Strait that serves as a seasonal insect migratory pathway in eastern Asia. We also simulated the migration trajectories of these pests using the HYSPLIT model. Overall, a total of 6362 alatae <jats:italic>S. miscanthi</jats:italic> and 335 alatae <jats:italic>R. padi</jats:italic> were captured, and both wheat aphid species exhibited regular patterns of comigration across the sea from late April to late October each year. There were significant interannual and seasonal differences in the migratory numbers of both wheat aphids. Notably, the average daily humidity was the most important meteorological factor affecting migration biomass. The seasonal migration trajectories simulated using the HYSPLIT model revealed different source areas for both wheat aphid species, which mostly originated in Northeast, North and East China. Accordingly, our study provides valuable insights into the occurrence and migration route of wheat aphids in eastern Asia, helps fine‐tune forecasting and early warning systems, and thereby guides integrated pest management of wheat aphids in eastern Asia.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Butterflies, bumblebees and hoverflies are equally effective pollinators of Knautia arvensis (Caprifoliaceae), a generalist plant species with compound inflorescences","authors":"Jeff Ollerton, Emma Coulthard, Sam Tarrant, James Woolford, Leonardo Ré Jorge, André Rodrigo Rech","doi":"10.1111/jen.13345","DOIUrl":"https://doi.org/10.1111/jen.13345","url":null,"abstract":"Plant‐pollinator interactions exist along a continuum from complete specialisation to highly generalised, that may vary in time and space. A long‐held assumption is that large bees are usually the most effective pollinators of generalist plants. We tested this by studying the relative importance of different groups of pollinators of <jats:italic>Knautia arvensis</jats:italic> (L.) Coult. (Caprifoliaceae: Dipsacoideae). This plant is suitable for such a study because it attracts a diversity of flower visitors, belonging to different functional groups. We asked whether all functional groups of pollinators are equally effective, or if one group is most effective, which has been documented in other species with apparently generalised pollination systems. We studied two subpopulations of <jats:italic>K. arvensis</jats:italic>, one at low and one at high density in Northampton, UK. To assess pollinator importance we exposed unvisited inflorescences to single visits by different groups of pollinators (butterflies, bumblebees, hoverflies and others) and assessed the proportion of pollinated stigmas. We then multiplied the effectiveness of each pollinator group with their proportional visitation frequency in five different years. For each group we also compared time spent on flowers and flight distance between visits. The relative importance of each pollinator group varied between years, as did their flight distances between flower visits. Butterflies were the best pollinators on a per visit basis (in terms of the proportion of stigmas pollinated) and flew further after visiting an inflorescence. Different measures and proxies of pollinator effectiveness varied between taxa, subpopulations, and years, and no one group of pollinators was consistently more effective than the others. Our results demonstrate the adaptive value of generalised pollination strategies when variation in relative abundance of different types of pollinators is considered. Such strategies may have buffered the ability of plants to reproduce during past periods of environmental change and may do so in the future.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of planting and harvest dates on yam infestation by white grubs","authors":"Romain Exilien, Robers Pierre Tescar, Holken Augustin, Dorensky Cadestin, Nicolas Carvil, Predner Duvivier, Gelin Doreus, Jessi Griesheimer","doi":"10.1111/jen.13348","DOIUrl":"https://doi.org/10.1111/jen.13348","url":null,"abstract":"The white grub, <jats:italic>Diaprepes abbreviatus</jats:italic> L. (Coleoptera: Curculionidae), is a significant pest of yam tubers and the second cause of tuber rot in Haiti. Larval feeding inflicts severe damage to tubers, leading to notable losses due to decreased tuber quality, reduced marketability and increased susceptibility to secondary parasites and pathogens. This study examined the influence of planting and harvesting dates on the vulnerability of yam tubers to white grub infestation and the resulting tuber quality across 24 farmers' fields. At harvest, we assessed the level of tuber infestation, white grub density and the tuber quality index. The results indicated that 85% of grub populations were found in fields planted on the traditional date. In addition, 69.5% of tuber damage occurred in early‐planted fields with fewer tubers damaged (30%) when harvested early. In contrast, in fields planted 30 days after the traditional date, the harvest date did not affect tuber infestation and damage by the pest. Overall, both planting and harvest dates impacted the quality grade of yam tubers. Delayed planting by 30 days and early harvesting (34th–38th week after planting) improved the marketability of yams. These findings highlight the importance of optimising planting and harvest schedules to better manage white grub infestation and enhance the marketability of yam tubers.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indoxacarb, cyantraniliprole, and Euborellia annulipes as options for integrated control of diamondback moth","authors":"Renilson Pessoa Morato, Deividy Vicente do Nascimento, Gemerson Machado Oliveira, Natalia Carolina Bermúdez, Rogério Lira, Jorge Braz Torres","doi":"10.1111/jen.13347","DOIUrl":"https://doi.org/10.1111/jen.13347","url":null,"abstract":"The diamondback moth (DBM), <jats:italic>Plutella xylostella</jats:italic> (L.), is responsible for extensive losses in brassicas' production, with its control heavily relying on the use of insecticides. The imbricated brassica's leaves on the stem provide a suitable habitat for the ring‐legged earwig, <jats:italic>Euborellia annulipes</jats:italic> (Lucas), to attack DBM larvae and pupae. The study focused on the combination of the earwig with indoxacarb and cyantraniliprole against DBM, as these insecticides target chewing and sucking sap pests infesting Brassica crops, respectively. These insecticides were tested against adult earwigs, 5th instar nymphs and predation upon DBM. When exposed to the recommended rates, the survival rates for nymphs were 74%, 100% and 96% in the cyantraniliprole, indoxacarb and control groups, respectively, and over 90% for adults. When 5th instar earwig nymphs were exposed to cyantraniliprole, they took 16.3 days to develop compared to indoxacarb (3.4 days) or the control group (3.2 days). Furthermore, females exposed to cyantraniliprole required more time to initiate oviposition and produced fewer eggs in the first egg batch. Despite that, the egg‐hatching rate was greater than 80% in all treatments. Female earwigs confined to indoxacarb‐ or cyantraniliprole‐treated or untreated leaves and DBM larvae maintained similar survival but lower consumption of DBM larvae when exposed to cyantraniliprole. The findings revealed that cyantraniliprole weakened the potential of the ring‐legged earwig to control DBM. On the other hand, indoxacarb was harmless to the earwig and showed additive mortality for the pest. Therefore, indoxacarb seems compatible with <jats:italic>E. annulipes</jats:italic>, while cyantraniliprole has a sublethal effect that slows down development and reproduction.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold tolerance of biological control agents Amblydromalus limonicus and Iphiseius degenerans","authors":"Samuel Musyoka Mbaka, Sasha Vasconcelos, Mohammad Hosein Rezai, Miriam Frida Karlsson, Mattias Jonsson","doi":"10.1111/jen.13346","DOIUrl":"https://doi.org/10.1111/jen.13346","url":null,"abstract":"Knowledge about cold tolerance of non‐native biological control agents is critical to avoid permanently establishing them in new temperate areas outside of their native range. The cold tolerance of the predatory mites, <jats:italic>Amblydromalus limonicus</jats:italic> and <jats:italic>Iphiseius degenerans</jats:italic>, was investigated in the laboratory to assess their establishment potential in northern Europe, particularly Sweden. The lethal time of <jats:italic>I. degenerans</jats:italic> (the number of days until 100% mortality was reached) declined steeply from 5°C to 0°C and was almost zero at −5°C. The lethal time of <jats:italic>A. limonicus</jats:italic> did not differ between 5°C and 0°C, but was reduced at −5°C. For both species, LTime<jats:sub>50</jats:sub> (the number of days until 50% of the mites died) was longer for fed than for unfed mites. The lethal temperature of <jats:italic>A. limonicus</jats:italic> (the temperature at which 100% mortality was reached) was −17.75°C, whereas most <jats:italic>I. degenerans</jats:italic> died at −8.5°C. LTemp<jats:sub>50</jats:sub> (the temperature at which 50% of the mites died) was lower for <jats:italic>A. limonicus</jats:italic> (−9.8°C) than for <jats:italic>I. degenerans</jats:italic> (−0.1°C). Collectively, these findings suggest that <jats:italic>I. degenerans</jats:italic> is unlikely to establish in Sweden but that <jats:italic>A. limonicus</jats:italic> is more cold tolerant. This highlights the risk associated with releasing <jats:italic>A. limonicus</jats:italic> in Sweden due to concerns about potential establishment.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An empirical model for predicting insects' diapause termination and phenology: An application to Cydia pomonella","authors":"Giorgio Sperandio, Sara Pasquali, Gianfranco Pradolesi, Serena Baiocco, Federico Cavina, Gianni Gilioli","doi":"10.1111/jen.13343","DOIUrl":"https://doi.org/10.1111/jen.13343","url":null,"abstract":"Diapause is a vital survival strategy for insects, enabling them to conserve energy and endure adverse conditions. Understanding how diapause affects insect phenology and population dynamics is crucial for the effective management of insect pests. Predictive pest phenological models can be invaluable tools for providing essential information to support management strategies. This study presents a modelling framework to incorporate diapause into phenological models when biological information on variables regulating and functions describing diapause induction and termination are lacking or limited. In our framework, insect phenology is divided into a set of phases characterized by specific events (diapause induction and termination) and processes (development of diapausing and post‐diapausing biological stages). The phenology is simulated by a stage‐structured model based on the Kolmogorov equation, and the temperature‐dependent development rate functions are described by the Brière functional form. Our modelling framework was tested on a case study involving the prediction of the phenology of the codling moth, (<jats:italic>Cydia pomonella</jats:italic> L. 1758). Model calibration and validation were performed using four time‐series adult trap catch data collected in the Emilia Romagna Region from 2021 to 2023. The calibration procedure allowed obtaining realistic parameters related to the temperature threshold triggering diapause termination and the development rate function of post‐diapausing larvae and pupae. Model validation proved successful in simulating both the initial emergence and the overall phenological patterns of adults across the three observed generations. The methodological framework proposed here aims to facilitate the introduction of diapause in phenological models improving also their predictive abilities. The model may serve as an accurate and knowledge‐based tool for planning and implementing pest monitoring and control actions based on the realistic predictions provided by the model on the phenological status of the pest.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous effective monitoring of Grapholita molesta and Cydia pomonella (Lepidoptera: Tortricidae) in traps with a dual sex pheromone/kairomone lure plus a UV‐A light","authors":"Alan Lee Knight, Valentina Mujica, Esteban Basoalto, Michele Preti","doi":"10.1111/jen.13344","DOIUrl":"https://doi.org/10.1111/jen.13344","url":null,"abstract":"Studies of oriental fruit moth (OFM), <jats:italic>Grapholita molesta</jats:italic> (Busck), and codling moth (CM), <jats:italic>Cydia pomonella</jats:italic> (L.) (Lepidoptera: Tortricidae), both important pests of pome fruit, evaluated the addition of low‐intensity light‐emitting diode (LED) to delta traps baited with 3 commercial monitoring lures. Studies were conducted in 9 orchards treated with and without sex pheromone mating disruption for one or both species. OFM was monitored with a two‐part pheromone/kairomone lure (OFM Combo Dual), while CM was monitored with either a two‐part kairomone lure (CM Dual 4 K) or a sex pheromone lure (CM PH). Both pests were monitored with or without the addition of three types of a low power LED light with peak intensity at 395 (UV‐A), 458 (blue), and 516 (green) nm. The UV‐A LED was found to be the most effective among the three lights for OFM. Unexpectedly, traps with the OFM Combo Dual lure plus UV‐A LED caught similar numbers of CM as traps baited with the CM Dual 4 K and CM PH lures alone. They also caught other pest species, such as oblique banded leafroller (OBLR), <jats:italic>Choristoneura rosaceana</jats:italic> (Harris), and peach twig borer (PTB), <jats:italic>Anarsia lineatella</jats:italic> (Zeller). Interestingly, in different trials the OFM Combo Dual combined with UV‐A LED light caught significantly more OFM and/or CM females compared to the OFM Combo Dual lure without light, opening the perspective for a female‐oriented monitoring and for a “female removal” management approach of these pests. The development of inexpensive, dual‐modality traps adding a light stimulus to a pheromone/kairomone lure may reduce monitoring costs in orchards with multiple moth pests, improving the trapping efficiency.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientific validation of plant extracts used by farmers in the management of bean flower thrips on French beans in Kenya","authors":"Nancy Mwende Munyoki, James Muthomi, Dora Kilalo, David Bautze, Milka Kiboi, Edwin Mwangi, Edward Karanja, Felix Matheri, Noah Adamtey, Komivi S. Akutse, Xavier Cheseto","doi":"10.1111/jen.13342","DOIUrl":"https://doi.org/10.1111/jen.13342","url":null,"abstract":"Bean flower thrips (<jats:italic>Megalurothrips usitatus</jats:italic>) is a major French bean (<jats:italic>Phaseolus vulgaris</jats:italic> L.) pest. Small‐scale farmers manage the pest using mixed plant extracts although their efficacy has not been scientifically validated. We evaluated the efficacy of mixed plant extracts comprising; <jats:italic>Capsicum frutescens</jats:italic>, <jats:italic>Allium sativum</jats:italic>, <jats:italic>Lantana camara</jats:italic>, <jats:italic>Tagetes minuta</jats:italic> and <jats:italic>Azadirachta indica</jats:italic>, against <jats:italic>M. usitatus</jats:italic> under laboratory and screenhouse. We identified and quantified the secondary metabolites associated with insecticidal activity using spectrophotometry and liquid chromatography–mass spectrometry (LC–MS). The plant combinations included PE1 (<jats:italic>C. frutescens + A. sativum + L. camara</jats:italic> + <jats:italic>T. minuta</jats:italic> extracts infused for 14 days), PE2 (same as PE1 but infused for 24 h) and PE + N (the five plant extracts infused for 24 h) in distilled water. We used an organic commercial botanical (Pyneem) as a positive control and distilled water as a negative control. Pyneem and PE + N induced the highest mortality at 88% and 77%, respectively, in the laboratory, and 68% and 71%, respectively, in the screenhouse. Phenolics, terpenoids and organosulfur compounds were identified in PE + N and individual plant extracts in varied quantities. These compounds were significantly higher (<jats:italic>p</jats:italic> &lt; 0.001) in PE + N compared to individual plant extracts. The study showed that PE + N efficiently manages bean flower thrips, and mixing different plant extracts amplifies the secondary metabolites' abundance. The use of mixed plant extracts could be incorporated into integrated pest management strategies for thrips management in legumes. The specific compounds identified in PE + N should be investigated further to understand their modes of action against the pest.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specificity of gut microbial community in three ecologically similar species of planthoppers with different host plant ranges","authors":"Yanhui Wang, Huirong Mai, Jingxian Zhou, Yuhan Zhang, Yanmin Liu, Yan Zou, Fajun Chen","doi":"10.1111/jen.13341","DOIUrl":"https://doi.org/10.1111/jen.13341","url":null,"abstract":"The three ecologically similar species of hemipteran sucking insects, brown planthopper (BPH) <jats:italic>Nilaparvata lugens</jats:italic>, small brown planthopper (SBPH) <jats:italic>Laodelphax striatellus</jats:italic> and white‐backed planthopper (WBPH) <jats:italic>Sogatella furcifera</jats:italic> are destructive pests causing severe damage to rice throughout Asia, but they have different host plants that BPH is monophagous insect just feeding rice, WBPH and SBPH are oligophagous insects additionally feeding wheat and barley and SBPH can also feed maize. This study was investigated the gut bacterial communities in BPH, WBPH and SBPH by high‐throughput amplicon sequencing in order to explain the differences in host range (SBPH&gt;WBPH&gt;BPH) and feeding habit (BPH with monophagy vs. WBPH and SBPH with oligophagy) of the three ecologically similar species of planthoppers. It was revealed that the gut bacterial flora in BPH was the most complex, and that in SBPH was the simplest. The diversity of the gut bacterial community in BPH was significantly higher than that in WBPH and SBPH, respectively, there was not only significant difference in α‐diversity metrics but also in β‐diversity metrics. KEGG enrichment analysis furtherly indicated that there was significantly different in the relative abundance of some functional categories (including those related to the biosynthesis of amino acids, fatty acids, lipids and carbohydrate) among BPH, WBPH and SBPH, which were closely related to their nutrient absorption and metabolism. It is presumed that the differences in abundance and composition of the gut bacteria in BPH, WBPH and SBPH may lead to different nutrient absorption and metabolism, which furtherly alter their host range and feeding habit.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142218163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity and compatibility studies of native Tolypocladium inflatum and Clonostachys krabiensis against Tetranychus urticae","authors":"Aditya Singh Ranout, Rupinder Kaur, Rahul Kumar, Gireesh Nadda","doi":"10.1111/jen.13339","DOIUrl":"https://doi.org/10.1111/jen.13339","url":null,"abstract":"The twospotted spider mite (TSSM), <jats:italic>Tetranychus urticae</jats:italic> Koch (Acari: Tetranychidae) is a major pest of field and greenhouse crops leading to qualitative and quantitative losses. Various chemical‐based acaricides are being used for its management that pose risks to human health, environment and non‐targeted organisms besides the development of resistance and resurgence of the pests. Therefore, alternative mite management practices are being promoted and implemented. Amongst them, entomopathogenic fungi (EPF) like <jats:italic>Beauveria bassiana</jats:italic> and <jats:italic>Metarhizium</jats:italic> spp. are being used globally, although new alternative EPF are required. Keeping this in mind, the present study was comprehended to determine the pathogenicity of native EPF, viz., <jats:italic>Tolypocladium inflatum</jats:italic> (Hypocreales: Ophiocordycipitaceae) and <jats:italic>Clonostachys krabiensis</jats:italic> (Hypocreales: Bionectriaceae) against different life stages of TSSM under laboratory conditions. The results indicated that adults are more vulnerable to studied fungi followed by nymphs and eggs of TSSM. The combined application of <jats:italic>T</jats:italic>. <jats:italic>inflatum</jats:italic> and <jats:italic>C</jats:italic>. <jats:italic>krabiensis</jats:italic> was significantly effective in controlling TSSM adults (99.33%) followed by <jats:italic>T</jats:italic>. <jats:italic>inflatum</jats:italic> (93.34%) and <jats:italic>C</jats:italic>. <jats:italic>krabiensis</jats:italic> (85.33%). According to the probit analysis, the combined application of studied EPF was found to be more effective against TSSM adults (LC<jats:sub>50</jats:sub> = 6.72 × 10<jats:sup>4</jats:sup> conidia/mL) followed by <jats:italic>T</jats:italic>. <jats:italic>inflatum</jats:italic> (LC<jats:sub>50</jats:sub> = 1.92 × 10<jats:sup>6</jats:sup> conidia/mL) and <jats:italic>C</jats:italic>. <jats:italic>krabiensis</jats:italic> (LC<jats:sub>50</jats:sub> = 7.90 × 10<jats:sup>6</jats:sup> conidia/mL). All three treatments at higher concentrations significantly reduced the adult and nymph populations. Morphological investigations using scanning electron microscopy revealed the successful conidial adhesion, germination and penetration of native <jats:italic>T</jats:italic>. <jats:italic>inflatum</jats:italic> and <jats:italic>C</jats:italic>. <jats:italic>krabiensis</jats:italic> conidia on TSSM adults. Thus, the acaricidal potential of isolated native fungi can further be explored for developing fungal‐based formulations for the sustainable management of mites.","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}