Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Effect of nanosized bioactive glass addition on some physical properties of biodentine.","authors":"Mehmet Burak Guneser,&nbsp;Tugce Yuca Ozturk,&nbsp;Asiye Nur Dincer Sahin,&nbsp;Betul Aycan Uysal,&nbsp;Ayce Unverdi Eldeniz","doi":"10.1177/22808000231184059","DOIUrl":"10.1177/22808000231184059","url":null,"abstract":"<p><p>The aim of this in vitro study was to investigate some physical properties of Biodentine (BD) (Septodont, France) that has been modified by adding nanosized bioactive glass (nBG) particles to it in different ratios. The cement was modified by adding 1% (7 mg) and 2% (14 mg) nBG powder to BD. BD was used as the control group in its commercial form. A total of 240 cement samples (<i>n</i> = 80) were prepared according to the standard measurements for each test. Subsequently, tests to determine compressive strength, microhardness, initial setting time, and solubility of the samples were performed. The obtained data were statistically analyzed using one-way ANOVA and Tukey's HDS tests, and the significance level was found to be 0.05. The compressive strength values of the samples modified with 1% and 2% nBG were higher than those of the unmodified BD; however, no statistically significant difference was found between them [BD + nBG (2 wt%) ⩾ BD+nBG (1 wt%) ⩾ control BD], (<i>p</i> > 0.05). The microhardness values of the samples modified with 1% and 2% nBG were found to be significantly higher than those of the control group [BD + nBG (2 wt%) > BD+nBG (1 wt%) > control BD], <i>p</i> < 0.05. Initial setting times were determined as 14 min for unmodified BD, 13 min for BD + nBG (1 wt%), and 12 min for BD + nBG (2 wt%). The addition of nBG to BD significantly reduced the initial setting time of BD (<i>p</i> < 0.05). A significant decrease was observed in the solubility of the BD modified with nBG samples compared to that of the control group [control BD > BD+nBG (1 wt%) >BD+nBG (2 wt%)], <i>p</i> < 0.05. Within the limitations of this study, it was found that the addition of certain amounts of nBG to BD positively affected some physical properties of the cement. Future in vitro and in vivo studies should be performed to prove the clinical applicability of the cements used in this study.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231184059"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10238837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of the remineralization characteristics of nano-hydroxyapatite extracted from fish scales and eggshells.","authors":"Sandile Cromwell Mkhize,&nbsp;Stanley Chibuzor Onwubu,&nbsp;Thabang Hendrica Mokhothu,&nbsp;Phumlane Selby Mdluli,&nbsp;Ajay Kumar Mishra","doi":"10.1177/22808000231180390","DOIUrl":"10.1177/22808000231180390","url":null,"abstract":"<p><strong>Objectives: </strong>Dentine hypersensitivity (DH) is a common concern in dentistry that has the potential to restrict daily activities and harm a person's quality of life. In this study, the remineralization characteristics of nano-hydroxyapatite (nHAp) extracted from waste eggshells and fish scales were comparatively assessed.</p><p><strong>Materials and methods: </strong>The extraction methods used to obtain nHAp from both fish scales and eggshells are also described. The effect of the extraction process and bio-waste source on the physicochemical characteristics of the nHAp such as Ca/P ratio, functional groups, crystallinity and phase change, and surface morphology are presented in the study. The remineralization properties were evaluated using dentine models (<i>n</i> = 15). A field scanning electron microscope was used to evaluate the effectiveness of the dentine tubules occlusion. The percentage occluded area for all the specimens was evaluated statistically using a one-way analysis of variance (α = 0.05).</p><p><strong>Results: </strong>The results showed that there were variations in the physicochemical characteristics of the nHAp extracted, including the crystallinity, particle size, and surface morphology, and buffering effects against citric acids. The EnHAp extracted from eggshells had higher crystallinity, superior buffering effects, and smaller particle size compared to the nHAp extracted from fish scales, making it a more favourable material for remineralization of teeth. The statistical evidence showed that there were statistically significant differences in the dentine occluding properties measured in the nHAp (<i>p</i> < 0.001). The highest mean % occluded area was measured with the EnHAp group.</p><p><strong>Conclusions: </strong>The findings of this study provide insights into the use of bio-waste materials for the development of sustainable and effective products for oral health care.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231180390"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10244115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene/hydroxyapatite coating deposit on titanium alloys for implant application.","authors":"Wufanbieke Baheti,&nbsp;ShangYi Lv,&nbsp;Mila,&nbsp;Lisha Ma,&nbsp;Dumanbieke Amantai,&nbsp;Hao Sun,&nbsp;HuiYu He","doi":"10.1177/22808000221148104","DOIUrl":"https://doi.org/10.1177/22808000221148104","url":null,"abstract":"<p><p>Titanium (Ti) implants are widely used in medicine. Meanwhile, surface modification of Ti can strengthen the osseointegration of implants. In this study, we modified Ti implant surfaces, which was coated with GO, HA, HA-2wt%GO and HA-5wt%GO via electrophoresis deposition, to investigate their mechanisms and biological activity. Uncoated Ti was used as the control. Further, we examined the biological behavior and osteogenic performance of mouse bone marrow mesenchymal stem cells (BMSCs) cultured on coatings in vitro. We found that the HA-GO nanocomposite coating improved the roughness and hydrophilicity of the Ti surface. Compared with the uncoated Ti or Ti modified by HA or GO alone, cell adhesion and diffusion were enhanced on HA-GO-modified Ti surfaces. In addition, the proliferation and osteogenic differentiation of BMSCs in vitro were significantly improved on HA-GO-modified surfaces, whereas osteogenesis-related gene expression and alkaline phosphatase activity were slightly enhanced. Furthermore, we noted that bone regeneration was improved in the HA-2wt%GO group in vivo. Thus, the HA-2wt%GO nanocomposite coating might have potential applications in the field of dental implants.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000221148104"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10516377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A methodology to evaluate different histological preparations of soft tissues: Intervertebral disc tissues study.","authors":"Azril,&nbsp;Kuo-Yuan Huang,&nbsp;Jonathan Hobley,&nbsp;Mehdi Rouhani,&nbsp;Wen-Lung Liu,&nbsp;Yeau-Ren Jeng","doi":"10.1177/22808000231155634","DOIUrl":"https://doi.org/10.1177/22808000231155634","url":null,"abstract":"<p><p>A tissue preparation method will inevitably alter the tissue content. This study aims to evaluate how different common sample preparation methods will affect the tissue morphology, biomechanical properties, and chemical composition of samples. The study focuses on intervertebral disc (IVD) tissue; however, it can be applied to other soft tissues. Raman spectroscopy synchronized with nanoindentation instrumentation was employed to investigate the compositional changes of IVD, specifically, nucleus pulposus (NP) and annulus fibrosus (AF), together with their biomechanical properties of IVD. These properties were examined through the following histological specimen types: fresh cryosection (control), fixed cryosection, and paraffin-embedded. The IVD tissue could be located using an optical microscope under three different preparation methods. Paraffin-embedded samples showed the most explicit details where the lamellae structure of AF could be identified. In terms of biomechanical properties, there was no significant difference between the fresh and fixed cryosection (<i>p</i> > 0.05). In contrast, the fresh cryosection and paraffin-embedded samples showed a significant difference (<i>p</i> < 0.05). It was also found that the tissue preparations affected the chemical content of the tissues and structure of the tissue, which are expected to contribute to biomechanical properties changes. Fresh cryosection and fixed cryosection samples are more promising to work with for biomechanical assessment in histological tissues. The findings fill essential gaps in the literature by providing valuable insight into the characteristics of IVD at the microscale. This study can also become a reference for a better approach to assessing the mechanical properties and chemical content of soft tissues at the microscale.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231155634"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9082604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of Sapindus Mukorossi extract with 17% EDTA on smear layer removal in various parts of the tooth root.","authors":"Asad Farooq, Afreen Bilgrami, Syed Abul Faraz, Fazal Ur Rehman Qazi, Afsheen Maqsood, Sara Altamash, Suraj Arora, Mohamad Syahrizal Halim, Naseer Ahmed, Gotam Das, Artak Heboyan","doi":"10.1177/22808000231166210","DOIUrl":"10.1177/22808000231166210","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to compare the effectiveness of an experimental root canal irrigant and 17% Ethylene-di-amine tetra acetic acid for removal of the smear layer in the coronal, middle and apical portions of the root canal.</p><p><strong>Materials and methods: </strong>Ninety human single rooted maxillary and mandibular teeth were selected for this study. The teeth were randomly divided into two experimental groups and one control group as follows: Group A (Ethanolic extract of Sapindus Mukorossi), Group B (17% EDTA), and Group C (Distilled water). The root canals of all three groups were prepared with stainless steel K-files by means of the standard step-back technique and irrigated with 5.25% sodium hypo chloride. The teeth were decoronated, following the irrigation and divided longitudinally into two-halves and visualized using scanning electron microscope (SEM) for the amount of smear layer present utilizing the three-point score system. The observations were noted both before and after the treatment. Nonparametric tests were applied for the comparison and <i>p</i>-value ⩽ 0.05 was considered as statistically significant.</p><p><strong>Results: </strong>It was evident from that smear layer was completely removed in coronal portion of 27 out of 30 teeth in-group A. For middle and apical areas of group A, 24 and 19 teeth showed complete smear layer removal. In-group B it was found that there were 24, 21, and 3 teeth at coronal, middle and apical, areas respectively where smear layer were completely absent. Intra group comparison showed a significant difference (<i>p</i> = 0.002) in smear layer removal was found for group A at coronal, middle and apical thirds. Similarly, a significant difference (<i>p</i> = 0.001) was also found for group B; however heavy smear layer was found among the three parts of the canal for group C.</p><p><strong>Conclusions: </strong>Ethanolic extract of Sapindus Mukorossi have higher effectiveness in removing the smear layer from the root canal in comparison to 17% EDTA.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231166210"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9283490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the content of Puerarin-PLGA nanoparticles in mice under the influence of alcohol and analysis of their antialcoholism.","authors":"Siyu Qiang,&nbsp;Lixiang Gu,&nbsp;Yu Kuang,&nbsp;Minyao Zhao,&nbsp;Yu You,&nbsp;Qin Han","doi":"10.1177/22808000221148100","DOIUrl":"https://doi.org/10.1177/22808000221148100","url":null,"abstract":"<p><p>To observe the metabolic changes and antialcoholic effect of Puerarin-PLGA nanoparticles (PUE-NP) in mice. PUE-NP was prepared and characterized by particle size distribution and morphology. The mouse models with acute alcoholism were established to observe their behavioral changes after alcohol poisoning. The expressions of biologically active enzymes such as CRE, BUN, AST, ALT in serum and SOD and TLR4 in liver of mice in each group were detected, and the pathological changes in liver and kidney tissues were observed by HE staining. The PUE-NP metabolism in mice was determined by in vitro release assay and HPLC. PUE-NP nanoparticles had good morphology and structure, and the mouse models with alcohol poisoning were established successfully. Compared with alcohol group, puerarin and PUE-NP increased the disappearance latency time of righting reflex, and the recovery time of righting reflex was significantly shortened. Water maze results showed that Puerarin and PUE-NP had inhibitory effect on impaired memory. HPLC results showed that PUE-NP reached its peak in mice after 1 h, and the content percentage was twice that of puerarin preparation alone, and the distribution time of puerarin concentration in vivo was prolonged, indicating that PLGA nanoparticles had a loading and slow-release effect on puerarin and increased the bioavailability of puerarin in mice. In addition, compared with the alcohol group, Puerarin and PUE-NP improved serum ALT, AST, CRE, and BUN levels in mice, enhanced SOD activity in liver, and inhibited TLR4 expression. The effect was better in the PUE-NP group than in the Puerarin group. PUE-NP delayed the release and metabolism of Puerarin and had better effect in the treatment of the alcoholic liver and kidney injury.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000221148100"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10588896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of titanium dioxide on <i>Streptococcus mutans</i> biofilm.","authors":"Molly K Sanders,&nbsp;Simone Duarte,&nbsp;Hadeel M Ayoub,&nbsp;Allison C Scully,&nbsp;LaQuia A Vinson,&nbsp;Richard L Gregory","doi":"10.1177/22808000221131892","DOIUrl":"https://doi.org/10.1177/22808000221131892","url":null,"abstract":"<p><strong>Background: </strong><i>Streptococcus mutans</i> (<i>S. mutan</i>s) participates in the dental caries process. Titanium dioxide (TiO₂) nanoparticles produce reactive oxygen species capable of disrupting bacterial DNA synthesis by creating pores in cell walls and membranes.</p><p><strong>Objective: </strong>The objective of this study was to determine the effect of TiO₂ on the disruption of <i>S. mutans</i> biofilm.</p><p><strong>Methods: </strong>This study was conducted in four phases involving a TiO₂-containing toothbrush and TiO₂ nanoparticles. Each phase was completed using 24 h established <i>S. mutans</i> biofilm growth. Phase one data was collected through a bacterial plating study, assessing biofilm viability. Biofilm mass was evaluated in phase two of the study by measuring <i>S. mutans</i> biofilm grown on microtiter plates following crystal violet staining. The third phase of the study involved a generalized oxygen radical assay to determine the relative amount of oxygen radicals released intracellularly. Phase four of the study included the measurement of insoluble glucan/extracellular polysaccharide (EPS) synthesis using a phenol-sulfuric acid assay.</p><p><strong>Results: </strong>Both exposure time and time intervals had a significant effect on bacterial viability counts (<i>p</i> = 0.0323 and <i>p</i> = 0.0014, respectively). Bacterial counts after 6 min of exposure were significantly lower than after 2 min (<i>p</i> = 0.034), compared to the no treatment control (<i>p</i> = 0.0056). As exposure time increased, the amount of remaining biofilm mass was statistically lower than the no treatment control. Exposure time had a significant effect on oxygen radical production. Both the 30 and 100 nm TiO₂ nanoparticles had a significant effect on bacterial mass. The silver nanoparticles and the 30 and 100 nm TiO₂ nanoparticles significantly inhibited EPS production.</p><p><strong>Conclusion: </strong>The TiO₂-containing toothbrush kills, disrupts, and produces oxygen radicals that disrupt established <i>S. mutans</i> biofilm. TiO₂ and silver nanoparticles inhibit EPS production and reduce biofilm mass. The addition of TiO₂ to dental products may be effective in reducing cariogenic dental biofilm.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000221131892"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9662905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of EGCG composite hydrogel for the treatment of radiation-induced skin injuries.","authors":"Jingying Wang, Lin Gao, Jianbo Song, Sijin Li","doi":"10.1177/22808000231218996","DOIUrl":"10.1177/22808000231218996","url":null,"abstract":"<p><p>With the rising incidence of cancer, radiotherapy has become an increasingly popular treatment modality. However, radiation-induced skin injuries (RSIs) have emerged as a commonly reported side effect of radiotherapy, thereby presenting a significant challenge in the field of radiotherapy. In this study, we report the successful synthesis of a photosensitive hydrogel via amide reaction for grafting the photosensitive group, methacrylate anhydride (MA), onto chitosan (CHI) and gelatin (GEL), with subsequent physical incorporation of epigallocatechin gallate (EGCG). The resulting composite photosensitive hydrogels exhibited favorable swelling properties, rheological properties, and biocompatibility, which promote angiogenesis and demonstrate notable therapeutic efficacy against RSIs. These findings provide valuable insights into the clinical utility of EGCG composite hydrogels for the effective management of RSIs.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231218996"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138829770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer.","authors":"Claudia María García-Cuellar,&nbsp;Rene Hernández-Delgadillo,&nbsp;Jesús Alejandro Torres-Betancourt,&nbsp;Juan Manuel Solis-Soto,&nbsp;Irene Meester,&nbsp;Yesennia Sánchez-Pérez,&nbsp;Nayely Pineda-Aguilar,&nbsp;Sergio Eduardo Nakagoshi-Cepeda,&nbsp;Rosa Isela Sánchez-Nájera,&nbsp;María Argelia Akemi Nakagoshi-Cepeda,&nbsp;Shankararaman Chellam,&nbsp;Claudio Cabral-Romero","doi":"10.1177/22808000231161177","DOIUrl":"https://doi.org/10.1177/22808000231161177","url":null,"abstract":"<p><strong>Objective: </strong>To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells.</p><p><strong>Material and methods: </strong>The human lung tumor cells A549 were exposed to 1-100 µM BisBAL NP or CPC, either separately or in a 1:1 combination. Cell viability was measured with the PrestoBlue assay, the LIVE/DEAD assay, and fluorescence microscopy. The integrity and morphology of cellular microtubules were analyzed by immunofluorescence.</p><p><strong>Results: </strong>A 24-h exposure to 1 µM solutions reduced A549 growth with 21.5% for BisBAL NP, 70.5% for CPC, and 92.4% for the combination (<i>p</i> < 0.0001), while a 50 µM BisBAL NP/CPC mixture inhibited cell growth with 99% (<i>p</i> < 0.0001). BisBAL NP-curcumin conjugates were internalized within 30 min of exposure and could be traced within the nucleus of tumor cells within 2 h. BisBAL NP, but not CPC, interfered with microtubule organization, thus interrupting cell replication, similar to the action mechanism of docetaxel.</p><p><strong>Conclusion: </strong>The growth inhibition of A549 human tumor cells by BisBAL NP and CPC was cumulative as of 1 µM. The BisBAL NP/CPC combination may constitute an innovative and cost-effective alternative for treating human lung cancer.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231161177"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9154408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-fungal efficacy of Miswak Extract (Salvadora Persica) and commercial cleaner against Candida albicans on heat cured polymethylmethacrylate denture base.","authors":"Nadia Wajdan, Kashif Aslam, Rafat Amin, Sadaf Khan, Naseer Ahmed, Abhishek Lal, Eman M AlHamdan, Fahim Vohra, Tariq Abduljabbar, Artak Heboyan","doi":"10.1177/22808000231165666","DOIUrl":"10.1177/22808000231165666","url":null,"abstract":"<p><strong>Aim: </strong>The aim of the study was to compare the antifungal efficacy between commercial cleaner (Corega) and Miswak extract (<i>Salvadora persica</i>) against <i>Candida albicans</i> on heat cured Polymethyl methacrylate (PMMA) acrylic denture base resin.</p><p><strong>Materials and methods: </strong>Forty-eight samples of heat cured PMMA acrylic denture base resin were fabircated in the study. The sterile acrylic resin specimens were immersed in standardized cell suspension of <i>Candida albicans</i> and incubated for 60 min at 370°C for cell adhesion and 2 h at 370°C for biofilm formation. After 24 h biofilm was evaluated by cell viability (CFUs) on SDA and cell counting of <i>Candida albicans</i> under light microscope at 400× magnification. The fungicidal effect of commercial cleaner and Miswak extract on <i>Candida albicans</i> biofilm was then evaluated by colony-forming units on SDA and cell counting under light microscope at 400× magnification.</p><p><strong>Results: </strong>Screening test agar disk-diffusion assay showed mean inhibitory zone of 3 mm for commercial cleaner as compared to Miswak extract, which showed mean inhibitory zone of 2 and 1 mm for different concentrations. Broth microdilution method showed 31 mg/ml MIC and 62.5 mg/ml Minimal Fungicidal Concentration (MFC) values for commercial cleaner as compared to Miswak extract that showed 125 mg/ml MIC and 250 mg/ml MFC values against <i>Candida albicans</i>. A significant difference (<i>p</i> < 0.05) was observed between pre and post treatment of both commercial cleaner and Miswak extract, for CFUs and cell count for <i>Candida albicans</i>.</p><p><strong>Conclusion: </strong>Commercial denture cleaner (Corega) showed better antifungal (<i>C albicans</i>) activity than Miswak extract (<i>Salvadora persica</i>) on heat cured PMMA acrylic denture base resin.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231165666"},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9752770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}