Journal of Applied Biomaterials & Functional Materials最新文献

{"title":"Polymeric hollow fibers for encapsulating <i>Eucalyptus galbie</i> essential oil with decongestant potential and physicochemical study.","authors":"Reyhane Shojaei, Mehdi Ansari, Neda Mohamadi, Fariba Sharififar","doi":"10.1177/22808000251335735","DOIUrl":"https://doi.org/10.1177/22808000251335735","url":null,"abstract":"<p><p><i>Eucalyptus</i> species are known for their decongestant and antimicrobial properties, mainly due to their essential oil. However, significant challenges persist in maintaining the stability and durability of the essential oils' effectiveness. This study examines the use of polymeric hollow fibers for encapsulating <i>Eucalyptus</i> essential oil (EE) to improve its stability and effectiveness in treating nasal congestion. The EE was obtained by hydro-distillation method, and analyzed using gas chromatography-mass spectrometry (GC-MS). A dialysis cartridge containing polysulfone hollow fibers was used to load the EE, and their permeability, morphology, and stability were assessed. Fourier-transform infrared spectroscopy (FT-IR) and headspace sampling with gas chromatography-flame ionization detection (GC-FID) were employed to monitor EE release and the stability of EE-loaded hollow fibers. GC/MS analysis identified 20 major components with 1,8-cineole being the predominant compound at 59.32%. Physicochemical characterization of hollow fibers revealed complete permeability to EE. FT-IR spectra suggested potential interactions between EE and the fibers. Release studies indicated that over 80% of EE was released from the fibers within 180 min. Headspace analysis confirmed the presence and stability of 1,8-cineole in the loaded fibers. The stability test demonstrated no significant changes in the EE-loaded fibers over 6 days. This study indicated that the capillary properties of the EE-loaded hollow fibers facilitate oil loading, and headspace sampling provides a more efficient analysis. The successful and stable release of EE from the fibers, highlights the potential of hollow fibers for controlled drug delivery. However, conducting more accurate experiments can help deduce more logical results.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251335735"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical characterization, cellular bioactivity, and biosafety evaluation of type A recombinant humanized collagen type XVII.","authors":"Nana Han, Xingxing Gao, Lu Song, Jian Wang, Ling Li, Kaixuan Guo, Huan Hu, Jing Wang, Bo Fan, Yun Zhu, Xia Yang","doi":"10.1177/22808000251389472","DOIUrl":"10.1177/22808000251389472","url":null,"abstract":"<p><p>Collagen type XVII is a unique transmembrane protein that plays a crucial role in anchoring epithelial cells to the underlying basement membrane. However, the low natural abundance of collagen type XVII in the human body poses significant challenges for large-scale extraction and production. With a development in biosynthesis technology, type A recombinant humanized collagen type XVII (rhCol XVII) has emerged as a promising and innovative alternative. In this study, rhCol XVII was successfully synthesized and expressed using genetic engineering techniques. The amino acid composition of rhCol XVII was analyzed, and its molecular weight was determined to be consistent with the theoretical design. Key physicochemical properties, including isoelectric point, particle size, and thermal stability, were evaluated. The experimentally measured pI was approximately 9.00, a particle size ranges from 820 to 857 nm, and thermal stability up to 23.60°C. The structure of rhCol XVII was characterized using infrared spectroscopy and circular dichroism. Furthermore, the cytocompatibility of rhCol XVII was assessed in vitro, and its systemic safety was evaluated in vivo. This research provided a solid foundation for the potential application of rhCol XVII in tissue engineering and regenerative medicine.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251389472"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145495300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pull-out bond strength and microleakage of lithium disilicate onlay with and without immediate dentin sealing.","authors":"Aruna Krishnan, Nishitha Arun, Mohammad Fareed, Nisha Manila, Mohmed Isaqali Karobari","doi":"10.1177/22808000251329112","DOIUrl":"https://doi.org/10.1177/22808000251329112","url":null,"abstract":"<p><strong>Background: </strong>Effective adhesion between dentin and resin materials is essential for the long-term durability of onlays, as the strength and durability of the tooth-restoration complex hinge on reliable adhesion. This study aims to enhance the adhesion of lithium disilicate onlays by employing immediate dentin sealing (IDS) and evaluates its effectiveness by comparing pull-out bond strength and microleakage with and without IDS.</p><p><strong>Methods: </strong>Extracted human premolars were prepared and categorized into two distinct groups: Group A (with IDS) and Group B (without IDS). In Group A, the dentin surfaces were sealed immediately after tooth preparation using an adhesive resin. In Group B, no sealing was performed before bonding the lithium disilicate restorations. Pull-out bond strength was assessed utilizing universal testing machine. Microleakage was evaluated through dye penetration analysis after thermocycling and sectioning of the samples. For statistical interpretation, one-way ANOVA followed by Tukey's post-hoc test was applied.</p><p><strong>Results: </strong>The pull-out bond strength was significantly higher in Group A compared to Group B (<i>p</i> = 0.0001). Microleakage evaluation revealed less dye penetration in Group A, indicating superior marginal integrity with IDS. The high-resolution images provided clearer visualization of the interfaces and the extent of dye penetration.</p><p><strong>Conclusion: </strong>Immediate dentin sealing significantly enhances the pull-out bond strength of lithium disilicate onlay and reduces microleakage. These findings suggest that IDS is a beneficial step in the bonding protocol for lithium disilicate restorations, potentially improving the longevity and performance of the restorations.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251329112"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cranioplasty infection in porous hydroxyapatite: Potential antibacterial properties.","authors":"Ismail Zaed, Corrado Iaccarino, Francesca Faedo, Laura Grillini, Elisabetta Galassi, Alessandro Dotti, Angelo Nataloni, Francesca Carolina Mannella, Andrea Cardia","doi":"10.1177/22808000241311389","DOIUrl":"10.1177/22808000241311389","url":null,"abstract":"<p><strong>Introduction: </strong>Intensive research is dedicated to the development of novel biomaterials and medical devices to be used as grafts in reconstructive surgery, with the purpose of enhancing their therapeutic effectiveness, safety, and durability. A variety of biomaterials, from autologous bone to polymethylmetacrylate, polyether ether ketone, titanium, and calcium-based ceramics are used in cranioplasty. Porous hydroxyapatite (PHA) is reported as a possible material for bone reconstruction, with good signs of biocompatibility, osteoconductive and osteointegrative properties. In the present paper we studied the possible antibacterial properties of PHA in a laboratory test in order to provide a possible overview of the occurrence of post-operative infections in PHA cranioplasty.</p><p><strong>Method: </strong>The test method has been designed to evaluate the potential antimicrobial activity of specimens under dynamic contact conditions to overcome difficulties in ensuring contact of inoculum to the specimen surface. The test was conducted using <i>Staphylococcus aureus</i> ATCC6538 as a bacterial strain.</p><p><strong>Results: </strong>Two experimental sets were performed to evaluate the antimicrobial properties of the specimens against two different <i>Staphylococcus aureus</i> concentrations. The first preliminary test (a) verified the antibacterial property at 0, 1, 2, and 4 h of contact time; the second confirmatory test (b) was repeated to verify the antibacterial property at 0, 4, 8 h. In the first experiment, after the first hour of contact, the bacterial inoculum was reduced by 7.96% compared to \"inoculum only,\" which increased to 26.11% at the second hour, and up to 52.33% after 4 h. In the second experiment, the confirmation test showed that bacterial growth reaches maximum inhibition after 4 h of contact. At 4 h, there was a higher bacterial reduction of 72.93%, which decreased at 8 h (36.45%).</p><p><strong>Conclusion: </strong>Analyzing the growth trend of viable microorganisms under Dynamic Contact Conditions it can be seen that PHA cranioplasty appears to inhibit exponential growth by inducing bacterial stasis in the early hours of contact, reaching a maximum reduction within 4 h, in this adopted experimental condition.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000241311389"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Less cytotoxic phthalocyanine derivative promotes in vitro wound healing compared to chlorhexidine.","authors":"Caique Andrade Santos, Adriano de Souza Pessoa, Fabiano Vieira Vilhena, Rodrigo Cardoso de Oliveira, Paulo Sérgio da Silva Santos, Carla Andreotti Damante, Mariana Schutzer Ragghianti Zangrando","doi":"10.1177/22808000251314630","DOIUrl":"10.1177/22808000251314630","url":null,"abstract":"<p><p>The use of adjunct chemical substances in the early postoperative period of periodontal surgical procedures is recommended due to the potential risk of trauma in the operated area. Chlorhexidine digluconate mouthwash is widely used but can cause adverse effects. Phthalocyanine derivatives are being studied as an alternative, demonstrating good antimicrobial activity, especially in the self-activated form, which does not require additional light or chemicals. The objective of this study is to compare the cytotoxicity of different concentrations of a phthalocyanine (PHY) with chlorhexidine (CHX) and assess their influence on fibroblast cell migration. Different concentrations of CHX and PHY (0.0075%-0.12%) were evaluated using NIH 3T3 fibroblasts. Cell viability was assessed by the MTT and crystal violet (CV) assay; CHX and PHY (0.0075% and 0.12%) were also evaluated by in vitro wound healing assay. PHY was less cytotoxic compared to CHX, based on cell viability assays. PHY did not interfere with experimental healing, allowing cell migration similar to the positive control with both concentrations (PHY 0.0075% and 0.12%) and only 0.0075% CHX allowed cell migration. In a comparative analysis, PHY showed less cytotoxicity than CHX and PHY concentrations of 0.0075% and 0.015% was non-toxic even after 48 h of contact with the cells. This in vitro evaluation demonstrated that PHY was less cytotoxic to NIH 3T3 fibroblasts compared to CHX. Furthermore, the different concentrations of PHY did not interfere negatively in the healing of experimental wounds.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251314630"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-layered electrospun grafts for surgical repair: Biomimicking physiological ascending aortic compliance.","authors":"Julia Krammer, Maximilian Pichlmaier, Jan Stana, Christian Hagl, Sven Peterss, Maximilian Grab, Linda Grefen","doi":"10.1177/22808000251316728","DOIUrl":"10.1177/22808000251316728","url":null,"abstract":"<p><p>Commercially available Dacron (woven polyester) grafts are used for routine open surgical repair of thoracic aortic aneurysms. Despite durable and biocompatible, these grafts do not reproduce the natural mechanical properties of the aorta. Therefore, the aim of this project was to develop an innovative graft that additionally exhibits physiological aortic compliance. To achieve this result, multi-layered tubular aortic grafts were created by electrospinning of a thermoplastic polyurethane. To reduce permeability, a gelatin-coating was added. Three groups (G_1-3; <i>n</i> = 5) with varying layer designs were evaluated regarding the main mechanical properties of vascular grafts such as suture retention strength, permeability and static and dynamic compliance. G₃, which combined electrospinning with a stable silicone-coated inlay was chosen for the fabrication of medical grade thermoplastic polyurethane grafts (G_m; <i>n</i> = 6). Dynamic compliance values of 19.68 ± 11.5%/100 mmHg (50-90 mmHg), 15.18 ± 8.7%/100 mmHg (80-120 mmHg) and 14.56 ± 7.4%/100 mmHg (110-150 mmHg) were achieved. The compliance was higher than for Dacron and ePTFE grafts and comparable to the normal sized ascending aorta of around 16%/100 mmHg in a healthy human and porcine aortic compliance of 14.3%/100 mmHg. Static compliance was successfully tested up to 350 mmHg. No significant changes in graft diameter or delaminations of the graft layers were detected after compliance testing. Therefore, by combining electrospinning with a durable inlay, both elasticity and recoverability are obtained, resulting in a promising alternative to the gold-standard in open-surgical treatment of thoracic aortic pathologies.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251316728"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amniotic membrane hydrogel as novel injectable platform in combination with metformin for treatment of sciatic nerve injury.","authors":"Zahra Rabiei Dolatabadi, Mona Saheli, Somayeh Solhjoo, Mehran Hosseini, Elahe Mousanejad, Mandana Jafari, Vahid Sheibani, Seyed Noureddin Nematollahi-Mahani","doi":"10.1177/22808000251322865","DOIUrl":"10.1177/22808000251322865","url":null,"abstract":"<p><p>Peripheral nerve tissue engineering is a field that uses cells, growth factors and biological scaffold material to provide a nutritional and physical support in the repair of nerve injuries. The specific properties of injectable human amniotic membrane-derived hydrogel including growth factors as well as anti-inflammatory and neuroprotective agents make it an ideal tool for nerve tissue repair, and metformin may also aid in nerve regeneration. The aim of this study was to investigate the effects of hydrogel derived from amniotic membrane (AM) along with metformin (MET) administration in the repair of sciatic nerve injury in male rats. We randomly divided 60 male rats into five groups. A control and four sciatic nerve compression groups including model; hydrogel; metformin and mix which received hydrogel and metformin. The recovery rate was assessed by Sciatic Functional Index (SFI), Static Sciatic Index (SSI) and von-frey test. Conduction velocity of the sciatic nerve was measured by Electrophysiological studies, and histological evaluations were performed 14 days after injury. SFI, SSI, latency time, remyelination rate and the expression of NF-200 and S-100β improved in hydrogel group. Response to mechanical stimulus, myelin density, axonal regeneration, and myelin sheath reconstruction improved in the mix group. The gastrocnemius muscle index was significantly reduced in the experimental groups while collagen fibers increased in these groups. These findings suggest that injection of hydrogel derived from decellularized amniotic membrane into the epineurium can be promoted reconstruction of peripheral nerve injury and improved functional nerve recovery. Also, metformin administration can reinforce the therapeutic effect of the hydrogel.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251322865"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of nano-chitosan and <i>Saussurea costus</i> as mouthwash and denture cleanser: In vitro study.","authors":"Sabreen Waleed Ibrahim, Farah Nabeel Mohammed Tahir AlKhayyat, Maha Jamal Abbas","doi":"10.1177/22808000251337545","DOIUrl":"https://doi.org/10.1177/22808000251337545","url":null,"abstract":"<p><strong>Introduction: </strong>Besides mechanical cleaning, mouthwashes, and denture cleansers are typical supplements for oral care. Many chemical mouthwashes and denture cleansers provide positive effects, but finding safe and natural alternatives should be the priority.</p><p><strong>Aim of study: </strong>This study aims to investigate the effectiveness of natural materials as mouthwash and denture cleansers.</p><p><strong>Material and method: </strong>In examining the antimicrobial efficacy of mouthwashes, the minimum inhibitory concentration values were determined for five groups: distilled water (negative control), 0.2% chlorhexidine, chitosan solution, <i>Saussurea costus</i> solution, and chitosan-Saussurea solution. As for denture cleansers, the efficacy was evaluated after immersing polymethylmethacrylate (PMMA) disks in the previous solutions. The cell viability of Human Gingival Fibroblast (hGFs) after treatment with tested solutions was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Data was analyzed using ANOVA with Bonferroni post hoc tests.</p><p><strong>Result: </strong>The prepared solutions showed comparable antimicrobial efficacy and cell viability as chlorohexidine. The chitosan-<i>Saussurea costus</i> solution demonstrated a higher antimicrobial effect and better prevention of microorganisms' adherence to PMMA disks and cell viability. It also did not affect the roughness of PMMA in the short term.</p><p><strong>Conclusion: </strong>The new denture cleaning and mouthwash agent, which contains the natural constituents of chitosan and <i>Saussurea costus</i>, demonstrates significant antibacterial effectiveness and serves as a viable eco-friendly alternative to commercial mouthwash, as well as exerting minimal influence on denture roughness.</p><p><strong>Clinical implications: </strong>Chitosan and <i>Saussurea costus</i> have antibacterial and anti-inflammatory properties that can potentially enhance oral health. These materials present a viable path toward the production of safe, efficient, and sustainable oral care products due to their natural and biocompatible substitutes.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251337545"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor and antibiofilm effect of a hydrogel loaded with octenidine hydrochloride (OCTH) for topical treatment of cancer.","authors":"Gustavo Martínez-González, Rene Hernández-Delgadillo, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Flores-Treviño, María Argelia Akemi Nakagoshi-Cepeda, Irene Meester, Juan Manuel Solis-Soto, Sergio Eduardo Nakagoshi-Cepeda, Claudio Cabral-Romero","doi":"10.1177/22808000251367864","DOIUrl":"https://doi.org/10.1177/22808000251367864","url":null,"abstract":"<p><strong>Objective: </strong>To determine and characterize the antitumor activity of a hydrogel loaded with octenidine hydrochloride (OCTH) and its action mechanism on human tumor cells.</p><p><strong>Material and methods: </strong>The antibiofilm property of OCTH against a clinical methicillin-resistant <i>Staphylococcus aureus</i> isolate (MRSA-CI) was evaluated by disk diffusion assays and a human bone-biofilm model in combination with the BacLight™ staining. The antitumor activity of soluble OCTH and OCTH-loaded biodegradable hydrogels was assessed in human tumor cell lines (MCF-7, CAL-27, FaDu, and A549) under different experimental conditions. Cell viability was measured with the MTT assay, and membrane permeability after OCTH-loaded hydrogel exposure was evaluated with the Calcein AM assay. Genotoxicity was evaluated with comet assay and fluorescence microscopy.</p><p><strong>Results: </strong>A stable, transparent, and biodegradable OCTH hydrogel with a pH of 7.2 was successfully developed. Biofilms exposed to 50 μm OCTH for 24 h exhibited only dead bacteria (red fluorescence), comparable to the effect of 100 μm vancomycin. Soluble OCTH demonstrated dose-dependent inhibition of tumor cell growth in all tested cell lines, with significant effects observed from 1 μm. The LD₅₀ values for soluble OCTH across the tumor cell lines ranged from 2.32 to 5.1 µm. Notably, hydrogel loaded with 50 μm OCTH significantly reduced cell growth by 44% after a 15-min exposure and by 97% after 120 min. The Calcein AM assay revealed that OCTH's antitumor mechanism involves the disruption of tumor cell plasma membrane permeability.</p><p><strong>Conclusion: </strong>This study is the first that describes the antitumor activity of octenidine hydrochloride on different human tumor cell lines. A biodegradable hydrogel loaded with octenidine hydrochloride constitutes an innovative, low-cost alternative for the topical treatment of cancer.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251367864"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication, characterization, and evaluation of a novel nano-ATP/chitosan membranes scaffold for bone regeneration.","authors":"Linxiang Zhang, Ting Dai, Xiaoyu Wu, Bin Yi, Hongbin Zhao","doi":"10.1177/22808000251345903","DOIUrl":"https://doi.org/10.1177/22808000251345903","url":null,"abstract":"<p><p>Guided tissue regeneration/guided bone regeneration membranes (GTR/GBR) are widely used to repair damaged bone, manufactured using various materials, including synthetic polymers, natural polymers, metals, and inorganic compounds. Our previous study showed that nano-attapulgite (nano-ATP) composite membranes could significantly increase bone regeneration. Here, the porous nano-ATP-incorporated CH membranes were fabricated via the technique of freeze gelation (FG). The property and thermal stability of membranes were tested by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). Cell proliferation and morphology on the membranes were also studied. In vivo Micro-CT and histological analyses were used to evaluate new bone formation in rat cranium defect models. The results indicated that with increasing ATP content, the water absorption, porosity, swelling rate, and degradation rate of chitosan membranes significantly decreased; conversely, the thermal weight loss and mechanical strength exhibited a marked increase. Culture of mouse mesenchymal cells (D1) showed that ATP-incorporated membranes increased cell proliferation and matrix deposition. Furthermore, the results from Micro-CT and histological staining demonstrated that ATP-incorporated membranes could increase new bone formation. These results suggest that this novel nano-ATP incorporated membrane has great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"23 ","pages":"22808000251345903"},"PeriodicalIF":3.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}