Journal of Advanced Research最新文献

{"title":"A novel navigation assistant method for substation inspection robot based on multisensory information fusion","authors":"Qiang Yang, Jingze Dong, Minyao Tan, Jiawei Wang, Dequan Guo, Hao Kang, Ping Wang","doi":"10.1016/j.jare.2025.01.016","DOIUrl":"https://doi.org/10.1016/j.jare.2025.01.016","url":null,"abstract":"<h3>Introduction</h3>Due to the complex environment of the substation, the inspection work of the substation becomes time-consuming and laborious. As a result, the substation inspection robot has gradually become a hot research point.<h3>Objectives</h3>At present, the mainstream intelligent inspection robots for substations use high-precision LiDAR sensors for navigation, which has high navigation accuracy but cannot identify the types of obstacles and the road contour boundary, seriously affecting the inspection performance and efficiency. Meanwhile, the high-precision 3D laser radar is too expensive to afford. In order to solve these problems, a novel navigation assistant method is proposed in this paper, which is based on multisensory information fusion.<h3>Methods</h3>Asynchronous information matching with multiple sensors was used to match the information collected by different sensors to deal with the time asynchrony. To express the height of obstacles, 2D laser radar was applied to create 3D imaging by being combined with inertial measurement unit (IMU). For perceiving and under-standing the substation environment independently by the inspection robot, ENet was given over to segment color point cloud maps, which was built by introducing optical sensor data. The method was implemented based on the ROS system and transplanted to embedded platform of the inspection robot.<h3>Result</h3>Finally, experimental results show that, compared with VLP-32C 3D laser radar sensors, the data volume of navigation assistance module had reduced by 95%. Meanwhile, after training the ENet network, the mean average accuracy value had achieved 86%, which meets the needs of practical engineering applications.<h3>Conclusion</h3>In addition, the inspection robot equipped with the navigation assistance module is successfully tested in multiple substations, which shows that the robot can not only identify the road contour and the type of obstacles on the way, but also reduce the amount of data and the cost of hardware.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"61 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High SERS performance of functionalized carbon dots in the detection of dye contaminants","authors":"Yanqiu Yang ,&nbsp;Lingru Kong ,&nbsp;Yong Ding ,&nbsp;Lixin Xia ,&nbsp;Shuo Cao ,&nbsp;Peng Song","doi":"10.1016/j.jare.2024.02.004","DOIUrl":"10.1016/j.jare.2024.02.004","url":null,"abstract":"<div><h3>Introduction</h3><div>The long-term overuse of malachite green (MG) has potential carcinogenic, teratogenic, and mutagenic effects. The functional nanocomposite is novel and challenging to construct and implement through surface enhanced Raman scattering (SERS) strategy to reveal the contributions in application.</div></div><div><h3>Objectives</h3><div>The novel Ag-CDs (carbon dots)-PBA (phenyl boric acid) nanocomposite was constructed by a facile route to detect toxic MG molecule with high SERS sensitivity and good uniformity.</div></div><div><h3>Methods</h3><div>The enhanced substrate used for the detection of MG has been successfully constructed using PBA modulated Ag-CDs on a structured surface with rich binding sites.</div></div><div><h3>Results</h3><div>The fabricated Ag-CDs-PBA substrate can be used to analyze various probe molecules exhibiting high sensitivity, good signal reproducibility, and excellent stability. The mechanism between components has been proved by calculations originating from the plasmonic Ag and active electronic transmission among the bridging CDs and PBA via the close spatial π-π effect. In addition, the accelerated separation of electron-hole pairs was triggered to further improve the SERS activity of the hybrid via a bidirectional charge transfer (CT) process. Significantly, the Ag-CDs-PBA system shows distinctive selectivity, in which PBA can hinder the interference of other species without specific hydroxyl groups.</div></div><div><h3>Conclusion</h3><div>Based on this deeper insight on plasmon-mediated mechanism, the SERS substrate was successfully practiced for quantitative determination in real water and fish samples. The strategy developed promises to be a new sensor technology and has great potential for environmental and food safety applications.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 89-98"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA hypomethylation-mediated upregulation of GADD45B facilitates airway inflammation and epithelial cell senescence in COPD","authors":"Yuan Zhan ,&nbsp;Qian Huang ,&nbsp;Zhesong Deng ,&nbsp;Shanshan Chen ,&nbsp;Ruonan Yang ,&nbsp;Jiaheng Zhang ,&nbsp;Yating Zhang ,&nbsp;Maocuo Peng ,&nbsp;Jixing Wu ,&nbsp;Yiya Gu ,&nbsp;Zhilin Zeng ,&nbsp;Jungang Xie","doi":"10.1016/j.jare.2024.02.005","DOIUrl":"10.1016/j.jare.2024.02.005","url":null,"abstract":"<div><h3>Introduction</h3><div>Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease typically characterized by chronic airway inflammation, with emerging evidence highlighting the driving role of cellular senescence-related lung aging. Accelerated lung aging and inflammation mutually reinforce each other, creating a detrimental cycle that contributes to disease progression. Growth arrest and DNA damage-inducible (GADD45) family has been reported to involve in multiple biological processes, including inflammation and senescence. However, the role of GADD45 family in COPD remains elusive.</div></div><div><h3>Objectives</h3><div>To investigate the role and mechanism of GADD45 family in COPD pathogenesis.</div></div><div><h3>Methods</h3><div>Expressions of GADD45 family were evaluated by bioinformatic analysis combined with detections in clinical specimens. The effects of GADD45B on inflammation and senescence were investigated via constructing cell model with siRNA transfection or overexpression lentivirus infection and animal model with Gadd45b knockout. Targeted bisulfite sequencing was performed to probe the influence of DNA methylation in GADD45B expression in COPD.</div></div><div><h3>Results</h3><div>GADD45B expression was significantly increased in COPD patients and strongly associated with lung function, whereas other family members presented no changes. GADD45B upregulation was confirmed in mice exposed by cigarette smoke (CS) and HBE cells treated by CS extract as well. Moreover, experiments involving bidirectional modulation of GADD45B expression in HBE cells further substantiated its positive regulatory role in inflammatory response and cellular senescence. Mechanically, GADD45B-facilitated inflammation was directly mediated by p38 phosphorylation, while GADD45B interacted with FOS to promote cellular senescence in a p38 phosphorylation-independent manner. Furthermore, Gadd45b deficiency remarkably alleviated inflammation and senescence of lungs in CS-exposed mice, as well as improved emphysema and lung function. Eventually, <em>in vivo</em> and <em>vitro</em> experiments demonstrated that GADD45B overexpression was partially mediated by CS-induced DNA hypomethylation.</div></div><div><h3>Conclusion</h3><div>Our findings have shed light on the impact of GADD45B in the pathogenesis of COPD, thereby offering a promising target for intervention in clinical settings.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 201-214"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139718155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A narrative review about cognitive impairment in Metabolic Dysfunction–Associated Steatotic Liver Disease (MASLD): Another matter to face through a holistic approach","authors":"Marica Meroni,&nbsp;Miriam Longo,&nbsp;Erika Paolini,&nbsp;Paola Dongiovanni","doi":"10.1016/j.jare.2024.02.007","DOIUrl":"10.1016/j.jare.2024.02.007","url":null,"abstract":"<div><h3>Background</h3><div>Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic hepatic disorder worldwide in both adults and children. It is well established that MASLD represents the hepatic manifestation of the metabolic syndrome whose definition includes the presence of obesity, type 2 diabetes (T2D), dyslipidemia, hypertension and hypercoagulability. All these conditions contribute to a chronic inflammatory status which may impact on blood brain barrier (BBB) integrity leading to an impaired function of central nervous system (CNS).</div></div><div><h3>Aim of review</h3><div>Since the mechanisms underlying the brain-liver-gut axis derangement are still inconclusive, the present narrative review aims to make a roundup of the most recent studies regarding the cognitive decline in MASLD also highlighting possible therapeutic strategies to reach a holistic advantage for the patients.</div></div><div><h3>Key Scientific Concepts of Review</h3><div>Due to its ever-growing prevalence, the MASLD-related mental dysfunction represents an enormous socio-economic burden since it largely impacts on the quality of life of patients as well as on their working productivity. Indeed, cognitive decline in MASLD translates in low concentration and processing speed, reduced memory, sleepiness but also anxiety and depression. Chronic systemic inflammation, hyperammonemia, genetic background and intestinal dysbiosis possibly contribute to the cognitive decline in MASLD patients. However, its diagnosis is still underestimated since the leading mechanisms are multi-faceted and unexplained and do not exist standardized diagnostic tools or cognitive test strategies. In this scenario, nutritional and lifestyle interventions as well as intestinal microbiota manipulation (probiotics, fecal transplantation) may represent new approaches to counteract mental impairment in these subjects.</div><div>In sum, to face the “mental aspect” of this multifactorial disease which is almost unexplored, cognitive tools should be introduced in the management of MASLD patients.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 231-240"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139901089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moscatilin inhibits vascular calcification by activating IL13RA2-dependent inhibition of STAT3 and attenuating the WNT3/β-catenin signalling pathway","authors":"Tingting Zhang ,&nbsp;Mengmeng Zhu ,&nbsp;Jialing Ma ,&nbsp;Zhenghong Liu ,&nbsp;Zhidan Zhang ,&nbsp;Meijie Chen ,&nbsp;Yaping Zhao ,&nbsp;Huaxin Li ,&nbsp;Shengnan Wang ,&nbsp;Xiaoning Wei ,&nbsp;Wenwen Zhang ,&nbsp;Xiaoxiao Yang ,&nbsp;Peter J. Little ,&nbsp;Danielle Kamato ,&nbsp;Hao Hu ,&nbsp;Yajun Duan ,&nbsp;Baotong Zhang ,&nbsp;Jianbo Xiao ,&nbsp;Suowen Xu ,&nbsp;Yuanli Chen","doi":"10.1016/j.jare.2024.02.020","DOIUrl":"10.1016/j.jare.2024.02.020","url":null,"abstract":"<div><h3>Introduction</h3><div>Vascular calcification, a devastating vascular complication accompanying atherosclerotic cardiovascular disease and chronic kidney disease, increases the incidence of adverse cardiovascular events and compromises the efficacy of vascular interventions. However, effective therapeutic drugs and treatments to delay or prevent vascular calcification are lacking.</div></div><div><h3>Objectives</h3><div>This study was designed to test the therapeutic effects and mechanism of Moscatilin (also known as dendrophenol) from <em>Dendrobium huoshanense</em> (an eminent traditional Chinese medicine) in suppressing vascular calcification <em>in vitro</em>, <em>ex vivo</em> and <em>in vivo</em>.</div></div><div><h3>Methods</h3><div>Male C57BL/6J mice (25-week-old) were subjected to nicotine and vitamin D₃ (VD₃) treatment to induce vascular calcification. <em>In vitro</em>, we established the cellular model of osteogenesis of human aortic smooth muscle cells (HASMCs) under phosphate conditions.</div></div><div><h3>Results</h3><div>By utilizing an in-house drug screening strategy, we identified Moscatilin as a new naturally-occurring chemical entity to reduce HASMC calcium accumulation. The protective effects of Moscatilin against vascular calcification were verified in cultured HASMCs. Unbiased transcriptional profiling analysis and cellular thermal shift assay suggested that Moscatilin suppresses vascular calcification via binding to interleukin 13 receptor subunit A2 (IL13RA2) and augmenting its expression. Furthermore, IL13RA2 was reduced during HASMC osteogenesis, thus promoting the secretion of inflammatory factors via STAT3. We further validated the participation of Moscatilin-inhibited vascular calcification by the classical WNT/β-catenin pathway, among which WNT3 played a key role in this process. Moscatilin mitigated the crosstalk between WNT3/β-catenin and IL13RA2/STAT3 to reduce osteogenic differentiation of HASMCs.</div></div><div><h3>Conclusion</h3><div>This study supports the potential of Moscatilin as a new naturally-occurring candidate drug for treating vascular calcification via regulating the IL13RA2/STAT3 and WNT3/β-catenin signalling pathways.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 445-457"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonic hedgehog restrains the ubiquitin-dependent degradation of SP1 to inhibit neuronal/glial senescence associated phenotypes in chemotherapy-induced peripheral neuropathy via the TRIM25-CXCL13 axis","authors":"Ying Zou ,&nbsp;Shu Wu ,&nbsp;Qian Hu ,&nbsp;Haoxian Zhou ,&nbsp;Yuanlong Ge ,&nbsp;Zhenyu Ju ,&nbsp;Shengkang Luo","doi":"10.1016/j.jare.2024.03.006","DOIUrl":"10.1016/j.jare.2024.03.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Chemotherapy-induced peripheral neuropathy (CIPN) is a common complication that affects an increasing number of cancer survivors. However, the current treatment options for CIPN are limited. Paclitaxel (PTX) is a widely used chemotherapeutic drug that induces senescence in cancer cells. While previous studies have demonstrated that Sonic hedgehog (Shh) can counteract cellular dysfunction during aging, its role in CIPN remains unknown.</div></div><div><h3>Objectives</h3><div>Herein, the aim of this study was to investigate whether Shh activation could inhibits neuronal/glial senescence and alleviates CIPN.</div></div><div><h3>Methods</h3><div>We treated ND7/23 neuronal cells and RSC96 Schwann cells with two selective Shh activators (purmorphamine [PUR] and smoothened agonist [SAG]) in the presence of PTX. Additionally, we utilized a CIPN mouse model induced by PTX injection. To assess cellular senescence, we performed a senescence-associated β-galactosidase (SA-β-gal) assay, measured reactive oxygen species (ROS) levels, and examined the expression of P16, P21, and γH2AX. To understand the underlying mechanisms, we conducted ubiquitin assays, LC-MS/MS, H&amp;E staining, and assessed protein expression through Western blotting and immunofluorescence staining.</div></div><div><h3>Results</h3><div><em>In vitro</em>, we observed that Shh activation significantly alleviated the senescence-related decline in multiple functions included SA-β-gal activity, expression of P16 and P21, cell viability, and ROS accumulation in DRG sensory neurons and Schwann cells after PTX exposure. Furthermore, our <em>in vivo</em> experiments demonstrated that Shh activation significantly reduced axonal degeneration, demyelination, and improved nerve conduction. Mechanistically, we discovered that PTX reduced the protein level of SP1, which was ubiquitinated by the E3 ligase TRIM25 at the lysine 694 (K694), leading to increased CXCL13 expression, and we found that Shh activation inhibited PTX-induced neuronal/glial senescence and CIPN through the TRIM25-SP1-CXCL13 axis.</div></div><div><h3>Conclusion</h3><div>These findings provide evidence for the role of PTX-induced senescence in DRG sensory neurons and Schwann cells, suggesting that Shh could be a potential therapeutic target for CIPN.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 387-402"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140117640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collecting duct NCOR1 controls blood pressure by regulating mineralocorticoid receptor","authors":"Ke Sun ,&nbsp;Yong-Li Wang ,&nbsp;Chen-Chen Hou ,&nbsp;Da Shang ,&nbsp;Lin-Juan Du ,&nbsp;Lan Bai ,&nbsp;Xing-Yu Zhang ,&nbsp;Chuan-Ming Hao ,&nbsp;Sheng-Zhong Duan","doi":"10.1016/j.jare.2024.02.003","DOIUrl":"10.1016/j.jare.2024.02.003","url":null,"abstract":"<div><h3>Introduction</h3><div>Nuclear receptor corepressor 1(NCOR1) is reported to play crucial roles in cardiovascular diseases, but its function in the kidney has remained obscure.</div></div><div><h3>Objective</h3><div>We aim to elucidate the role of collecting duct NCOR1 in blood pressure (BP) regulation.</div></div><div><h3>Methods and Results</h3><div>Collecting duct NCOR1 knockout (KO) mice manifested increased BP and aggravated vascular and renal injury in an angiotensin II (Ang II)-induced hypertensive model. KO mice also showed significantly higher BP than littermate control (LC) mice in deoxycorticosterone acetate (DOCA)-salt model. Further study showed that collecting duct NCOR1 deficiency aggravated volume and sodium retention after saline challenge. Among the sodium transporter in the collecting duct, the expression of the three epithelial sodium channel (ENaC) subunits was markedly increased in the renal medulla of KO mice. Consistently, BP in Ang II-infused KO mice decreased significantly to the similar level as those in LC mice after amiloride treatment. ChIP analysis revealed that NCOR1 deficiency increased the enrichment of mineralocorticoid receptor (MR) on the promoters of the three ENaC genes in primary inner medulla collecting duct (IMCD) cells. Co-IP results showed interaction between NCOR1 and MR, and luciferase reporter results demonstrated that NCOR1 inhibited the transcriptional activity of MR. Knockdown of MR eliminated the increased ENaC expression in primary IMCD cells isolated from KO mice. Finally, BP was significantly decreased in Ang II-infused KO mice after treatment of MR antagonist spironolactone and the difference between LC and KO mice was abolished.</div></div><div><h3>Conclusions</h3><div>NCOR1 interacts with MR to control ENaC activity in the collecting duct and to regulate sodium reabsorption and ultimately BP. Targeting NCOR1 might be a promising tactic to interrupt the volume and sodium retention of the collecting duct in hypertension.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 75-87"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis","authors":"Hong Wang ,&nbsp;Dong Qian ,&nbsp;Jiabei Wang ,&nbsp;Yao Liu ,&nbsp;Wenguang Luo ,&nbsp;Hongyan Zhang ,&nbsp;Jingjing Cheng ,&nbsp;Heng Li ,&nbsp;Yang Wu ,&nbsp;Wuhan Li ,&nbsp;Jing Wang ,&nbsp;Xia Yang ,&nbsp;Tianzhi Zhang ,&nbsp;Dong Han ,&nbsp;Qinyao Wang ,&nbsp;Chris Zhiyi Zhang ,&nbsp;Lianxin Liu","doi":"10.1016/j.jare.2024.02.010","DOIUrl":"10.1016/j.jare.2024.02.010","url":null,"abstract":"<div><h3>Introduction</h3><div>Abnormal alternative splicing (AS) contributes to aggressive intrahepatic invasion and metastatic spread, leading to the high lethality of hepatocellular carcinoma (HCC).</div></div><div><h3>Objectives</h3><div>This study aims to investigate the functional implications of UPF3B-S (a truncated oncogenic splice variant) in HCC metastasis.</div></div><div><h3>Methods</h3><div>Basescope assay was performed to analyze the expression of UPF3B-S mRNA in tissues and cells. RNA immunoprecipitation, and in vitro and in vivo models were used to explore the role of UPF3B-S and the underlying mechanisms.</div></div><div><h3>Results</h3><div>We show that splicing factor HnRNPR binds to the pre-mRNA of UPF3B via its RRM2 domain to generate an exon 8 exclusion truncated splice variant UPF3B-S. High expression of UPF3B-S is correlated with tumor metastasis and unfavorable overall survival in patients with HCC. The knockdown of UPF3B-S markedly suppresses the invasive and migratory capacities of HCC cells in vitro and in vivo. Mechanistically, UPF3B-S protein targets the 3′-UTR of CDH1 mRNA to enhance the degradation of CDH1 mRNA, which results in the downregulation of E-cadherin and the activation of epithelial–mesenchymal transition. Overexpression of UPF3B-S enhances the dephosphorylation of LATS1 and the nuclear accumulation of YAP1 to trigger the Hippo signaling pathway.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that HnRNPR-induced UPF3B-S promotes HCC invasion and metastasis by exhausting CDH1 mRNA and modulating YAP1-Hippo signaling. UPF3B-S could potentially serve as a promising biomarker for the clinical management of invasive HCC.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 257-270"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139975144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achieving healthy aging through gut microbiota-directed dietary intervention: Focusing on microbial biomarkers and host mechanisms","authors":"Yue Xiao ,&nbsp;Yingxuan Feng ,&nbsp;Jianxin Zhao ,&nbsp;Wei Chen ,&nbsp;Wenwei Lu","doi":"10.1016/j.jare.2024.03.005","DOIUrl":"10.1016/j.jare.2024.03.005","url":null,"abstract":"<div><h3>Background</h3><div>Population aging has become a primary global public health issue, and the prevention of age-associated diseases and prolonging healthy life expectancies are of particular importance. Gut microbiota has emerged as a novel target in various host physiological disorders including aging. Comprehensive understanding on changes of gut microbiota during aging, in particular gut microbiota characteristics of centenarians, can provide us possibility to achieving healthy aging or intervene pathological aging through gut microbiota-directed strategies.</div></div><div><h3>Aim of Review</h3><div>This review aims to summarize the characteristics of the gut microbiota associated with aging, explore potential biomarkers of aging and address microbiota-associated mechanisms of host aging focusing on intestinal barrier and immune status. By summarizing the existing effective dietary strategies in aging interventions, the probability of developing a diet targeting the gut microbiota in future is provided.</div></div><div><h3>Key Scientific Concepts of Review</h3><div>This review is focused on three key notions: Firstly, gut microbiota has become a new target for regulating health status and lifespan, and its changes are closely related to age. Thus, we summarized aging-associated gut microbiota features at the levels of key genus/species and important metabolites through comparing the microbiota differences among centenarians, elderly people and younger people. Secondly, exploring microbiota biomarkers related to aging and discussing future possibility using dietary regime/components targeted to aging-related microbiota biomarkers promote human healthy lifespan. Thirdly, dietary intervention can effectively improve the imbalance of gut microbiota related to aging, such as probiotics, prebiotics, and postbiotics, but their effects vary among.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 179-200"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin blocks BIK1-mediated CPK28 phosphorylation and enhances plant immunity","authors":"Yazhou Bao ,&nbsp;Qian Zhang ,&nbsp;Hai Zhu ,&nbsp;Yong Pei ,&nbsp;Yaning Zhao ,&nbsp;Yixin Li ,&nbsp;Peiyun Ji ,&nbsp;Dandan Du ,&nbsp;Hao Peng ,&nbsp;Guangyuan Xu ,&nbsp;Xiaodan Wang ,&nbsp;Zhiyuan Yin ,&nbsp;Gan Ai ,&nbsp;Xiangxiu Liang ,&nbsp;Daolong Dou","doi":"10.1016/j.jare.2024.02.025","DOIUrl":"10.1016/j.jare.2024.02.025","url":null,"abstract":"<div><h3>Introduction</h3><div>Metformin (MET), derived from <em>Galega officinalis</em>, stands as the primary first-line medication for the treatment of type 2 diabetes (T2D). Despite its well-documented benefits in mammalian cellular processes, its functions and underlying mechanisms in plants remain unclear.</div></div><div><h3>Objectives</h3><div>This study aimed to elucidate MET’s role in inducing plant immunity and investigate the associated mechanisms.</div></div><div><h3>Methods</h3><div>To investigate the impact of MET on enhancing plant immune responses, we conducted assays measuring defense gene expression, reactive oxygen species (ROS) accumulation, mitogen-activated protein kinase (MAPK) phosphorylation, and pathogen infection. Additionally, surface plasmon resonance (SPR) and microscale thermophoresis (MST) techniques were employed to identify MET targets. Protein-protein interactions were analyzed using a luciferase complementation assay and a co-immunoprecipitation assay.</div></div><div><h3>Results</h3><div>Our findings revealed that MET boosts plant disease resistance by activating MAPKs, upregulating the expression of downstream defense genes, and fortifying the ROS burst. CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28) was identified as a target of MET. It inhibited the interaction between BOTRYTIS-INDUCED KINASE 1 (BIK1) and CPK28, blocking CPK28 threonine 76 (T76) transphosphorylation by BIK1, and alleviating the negative regulation of immune responses by CPK28. Moreover, MET enhanced disease resistance in tomato, pepper, and soybean plants.</div></div><div><h3>Conclusion</h3><div>Collectively, our data suggest that MET enhances plant immunity by blocking BIK1-mediated CPK28 phosphorylation.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"68 ","pages":"Pages 31-41"},"PeriodicalIF":11.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}