Kaichong Teng, Neng Zhao, Yonghong Xie, Rongbai Li, Jianxiong Li
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引用次数: 0
摘要
引言水稻的小花是小穗的主要组成部分,而雌蕊的胚珠是成功繁殖的关键器官,决定着种子的数量。目的双苗水稻在水稻生产中具有巨大的应用潜力。方法 我们发现了双苗水稻(tsr)突变体,并利用基于图谱的克隆方法构建了不同的分离群体来克隆 TSR 基因。为了探索TSR在决定子房数量和发育中的分子功能,我们应用酵母双杂交实验、电泳迁移实验(EMSA)和双LUC瞬时表达实验等分子技术寻找TSR的相互作用蛋白和受TSR调控的靶基因。TSR 基因突变导致双苗水稻的出现。tsr突变体的小穗呈开壳状,小花的雄蕊和胚珠数量也发生了变化。tsr突变体的子房中有两个结合胚珠,这些胚珠发育成带有两个可存活胚的成熟种子。机理研究发现,TSR 通过与小穗确定和胚珠特征相关基因的启动子结合来调节这些基因的表达水平。此外,TSR 与 OsMADS1 相互作用,这种作用使 OsMADS1 能够调节 TSR 对基因表达的转录活动。对TSR的分子研究为水稻小花的形成和发育提供了新的见解,有助于育种者在生产中培育双苗水稻。
An AP2/ERF transcription factor controls generation of the twin-seedling rice
Introduction
The floret of rice is a main component of the spikelet, and the ovule of pistil is a critical organ for successful reproduction and determines the number of seeds. However, the molecular mechanisms underlying the ovule development remain elusive.
Objective
Twin-seedling rice has great potential for application in rice production. The study was to isolate the gene that controls twin-seedling in rice and explore the molecular function of the gene in floret development.
Methods
We discovered a twin-seedling rice (tsr) mutant and constructed different segregating populations to clone TSR gene using map-based cloning method. To explore the molecular functions of TSR in determination of the ovary number and development, we applied molecular technologies such as yeast two-hybrid assay, electrophoretic mobility shift assay (EMSA), and dual-LUC transient expression assay to search for the TSR-interacting proteins and the target genes regulated by TSR.
Results
We report here the map-based cloning of TSR which encodes an AP2/ERF transcription factor. Mutations in TSR lead to occurrence of the twin-seedling rice. The tsr mutant showed open hulls of the spikelets and displayed changes in the number of stamens and ovules of the florets. The ovary of tsr mutant contained two conjugated ovules which developed into a mature seed with two viable embryos. Mechanistic studies revealed that TSR regulates the expression levels of genes related to spikelet determination and ovule identity by binding to their promoters. Furthermore, TSR interacted with OsMADS1 and this interaction allowed OsMADS1 to modulate the transcriptional activities of TSR on gene expression. The molecular study of TSR provides new insights into the formation and development of rice floret and helps breeders generate twin-seedling rice in production.
期刊介绍:
Journal of Advanced Research (J. Adv. Res.) is an applied/natural sciences, peer-reviewed journal that focuses on interdisciplinary research. The journal aims to contribute to applied research and knowledge worldwide through the publication of original and high-quality research articles in the fields of Medicine, Pharmaceutical Sciences, Dentistry, Physical Therapy, Veterinary Medicine, and Basic and Biological Sciences.
The following abstracting and indexing services cover the Journal of Advanced Research: PubMed/Medline, Essential Science Indicators, Web of Science, Scopus, PubMed Central, PubMed, Science Citation Index Expanded, Directory of Open Access Journals (DOAJ), and INSPEC.