Journal of Animal Physiology and Animal Nutrition最新文献

{"title":"Effects of Corn Distillers Dried Grains With Soluble as a Partial Replacement for Soybean Meal Improve the Growth Performance and Apparent Total Tract Digestibility of Weaning Pigs.","authors":"Muniyappan Madesh, Demin Cai, In Ho Kim","doi":"10.1111/jpn.14113","DOIUrl":"https://doi.org/10.1111/jpn.14113","url":null,"abstract":"<p><p>This study investigated the impact of corn distillers' dried grains with solubles (cDDGS) on growth performance, faecal score and apparent total tract digestibility (ATTD) in weaning pigs by replacing soybean meal (SBM) in the diet. Ninety-six (Duroc × [Landrace × Yorkshire]) weaning pigs with an average body weight (BW) of 6.37 ± 1.10 kg were randomly to 3 groups in a randomized complete design with 8 pens/group and 4 pigs/pen (2 barrows and 2 gilts). Three diets (cDDGS0, cDDGS2.5 and cDDGS5) were formulated using cDDGS to replace 0%, 2.5% and 5% of SBM in the diet. Results found that increasing cDDGS inclusion linearly increased (p < 0.05) the BW, average daily gain (ADG), average daily feed intake (ADFI) and ATTD of dry matter (DM), crude protein (CP), and gross energy (GE) and linearly reduced (p < 0.05) faecal score. However, feed conversion ratio (FCR) had no significant difference among treatments. In conclusion, cDDGS improved the growth performance and ATTD and reduced faecal score in weaning pigs, and the appreciable benefits seen in the 5% cDDGS group.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prebiotic Oligosaccharides as Potential Growth Promoter in Rabbits: A Review.","authors":"Ayman Hassan Abd El-Aziz, Daniel Mota-Rojas, Obafemi F Akinjute, Monsuru O Abioja","doi":"10.1111/jpn.14114","DOIUrl":"https://doi.org/10.1111/jpn.14114","url":null,"abstract":"<p><p>Raising rabbit on commercial scale is a significant industry for the production of meat, fur and leather. Rabbits are known to possess high fecundity and good quality meat. The animals utilise low-quality, low-cost diets like forages and agricultural by-products. However, growing rabbits are very prone to digestive problems that cause appetite loss, diarrhoea, slower growth rate and higher mortality rate, especially during the weaning period. Antibiotic growth promoters (AGP), which had been used comprehensively, are avoided because of resultant development of drug resistance in animals and antibiotic residues in animal products. As a result, alternative dietary healthy growth promoters are in high demand. Prebiotics and probiotics are two growth promoters that could replace AGP since they boost effectiveness without having any negative effects on intake. Utilising drugs or antibiotics to produce rabbit resulted in higher final product costs from an economic perspective. Prebiotics are non-fermentable, ingestible fibres and sugars, such as inulin-type fructans, mannan-oligosaccharides (MOS), isomalto-oligosaccharides (IMO), and galacto-oligosaccharides (GOS), that show immune-stimulating qualities to the host by favourably supporting the growth of some helpful bacteria (probiotics). For instance, supplementation of 3.0 g MOS/kg and 0.5 g IMO/kg to the diet of rabbits has been recommended to improve productivity in rabbits. Numerous studies also demonstrate the positive effects of dietary prebiotics supplements on the gut health, productivity and immunity of broilers. Thus, this review aimed to summarising the results in literature and to draw attention of the stakeholders in rabbit production to the potential impacts of some commercial prebiotics, such as MOS and IMO, on growth effectiveness, carcass characteristics, blood biochemistry, intestinal tract histomorphometries, and the expression of specific relative immune or growth genes.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Forage-Free Diet on the Growth Performance, Feed Efficiency, Blood Metabolites, and Mammary Gland Parenchyma of Prepubertal Holstein Heifers.","authors":"Muhammad Ahmad, Shafiq Ur Rehman, Muhammad Zafar Bashir, Muhammad Akram, Arslan Qayyum, Muhamad Naveed-Ul-Haque, Imran Mohsin, Muhammad Oneeb, Ghulam Mustafa, Muhammad Usman Mehmood, Ghazanfar Ali Chishti","doi":"10.1111/jpn.14108","DOIUrl":"https://doi.org/10.1111/jpn.14108","url":null,"abstract":"<p><p>Variation in nutritional profile and availability of high-quality forages remain primary challenges for efficient heifer rearing on a forage-based diet. This study aimed to evaluate the effect of limit feeding forage-free diet on growth performance, feed efficiency, blood metabolites, behavior, health, and mammary parenchyma of prepubertal dairy heifers. Sixteen prepubertal heifers (Body weight = 168 ± 32Kg) divided into 8 pens (2 heifers/pen) were used in a completely randomized design (n = 4 pen/treatment) to compare the effect of a diet containing 20% forage with a forage-free diet. Limit feeding forage-free diet improved the feed efficiency by 10% and ADG by 11.36% (p ≤ 0.05) without negatively affecting the body measurements, health parameters, and blood metabolites (p ≥ 0.23). In udder, three-quarters of heifers fed a forage-free diet had greater (p ≤ 0.05) mammary parenchyma growth than the heifers fed a 20% forage diet. Teat lengths and behavioral measurements except rumination time were not affected (p > 0.05) by the forage-free diet. Heifers fed a 20% forage diet had greater rumination time (p = 0.01) than the heifers fed a forage-free diet. Limit feeding forage-free diet can be adopted in prepubertal dairy heifers for the improvement in feed efficiency and mammary parenchyma growth.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Different Metabolizable Energy Diets on Broilers' Growth Performance, Nutrient Digestibility, Organ Weight, Fecal Score and Lesion Score.","authors":"Usman Kayode Kolawole, In Ho Kim","doi":"10.1111/jpn.14110","DOIUrl":"https://doi.org/10.1111/jpn.14110","url":null,"abstract":"<p><p>This study was conducted to investigate the effect of diets with different metabolizable energy (ME) levels on broiler growth performance, nutrient digestibility, fecal score, organ weight and lesion score. A total of 1134 1-day-old Ross broiler chicks with an initial body weight (BW) of 44.33 ± 1.70 g were randomly assigned to three dietary treatment groups. There were 21 replicate pens per treatment, including 18 male birds per pen. The experimental period was divided into 3 phases and the diets were provided to broilers from days 1 to 34 of age: the starter (days 1-7), growing (days 8-20), and finishing (days 21-34). The dietary treatments included CON, basal diet (3200 Kcal/kg), TRT1, basal diet - 50 kcal/kg ME (3150 Kcal/kg), and TRT2, basal diet + 50 kcal/kg ME (3250 kcal/kg). The TRT2 group showed a reduced (p < 0.05) feed conversion ratio (FCR) compared to the TRT1 group during the growing phase. During the overall experimental period, TRT2 group broilers showed an increased (p < 0.05) average daily gain (ADG) and a reduced (p < 0.05) FCR than in the TRT1 group. However, there were no differences observed in feed intake (FI) and body weight gain (BWG), nutrient digestibility, fecal score, organ weight and lesion score. In conclusion, feeding a diet at 3250 kcal/kg ME from days 1-34 could be suitable for improving both average daily gain and feed conversion ratio in broiler chickens.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in Milk Biomolecular Profiles and Piglet Performances Due to Dietary Probiotic Bacillus licheniformis DSMZ 28710 Supplementation.","authors":"Jakavat Ruampatana, Sarn Settachaimongkon, Jedsadakorn Kaewsirikool, Pornpavit Iamraksa, Sirawit Choompoo, Junpen Suwimonteerabutr, Kunaporn Homyog, Wanwimon Mekboonsonglarp, Takele Feyera, Morakot Nuntapaitoon","doi":"10.1111/jpn.14109","DOIUrl":"https://doi.org/10.1111/jpn.14109","url":null,"abstract":"<p><p>The present study aimed to investigate the effects of probiotic Bacillus licheniformis DSMZ 28710 supplementation on sow performance, Preweaning piglet performance, and the biochemical profiles of colostrum and milk. Sixty-eight crossbred sows (Landrace <math> <semantics> <mrow><mrow><mo>×</mo></mrow> </mrow> <annotation>$times $</annotation></semantics> </math> Yorkshire) were allocated to either a standard lactation diet (Control; n = 35) or the Control diet supplemented with 10 g/sow/day of B. licheniformis DSMZ 28710 (Treatment; n = 33), from day 109 of gestation until day 21 of lactation. Sow and piglet performance, as well as the incidence of piglet diarrhea, were recorded. Moreover, the study investigated the changes in major chemical compositions, immunoglobulins, fatty acids, and non-volatile polar metabolites in colostrum, transient milk, and mature milk of sows. Supplementation of B. licheniformis increased piglet body weight on day 21 of lactation in old parity sows (p = 0.037). Moreover, the incidence of diarrhea was reduced in piglets suckled by sows supplemented with B. licheniformis DSMZ 28710, regardless of sow's parity or lactation stage (p < 0.05). Probiotic supplementation decreased fat content in transient milk (p = 0.026) and increased lactose content in mature milk (p = 0.011). Chemometric analysis revealed clear distinctions between the Control and Treatment group in the fatty acid profiles of colostrum, transient milk, and mature milk, while notable differences in non-volatile polar metabolite profiles were observed specifically in mature milk. In conclusion, supplementation with B. licheniformis DSMZ 28710 reduced the incidence of diarrhea in piglets, increased body weight of the piglets in old parity sows and altered the biomolecular profiles in colostrum, transit milk, and mature milk of the sows.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Plant-Derived Feed Additives on Enteric Methane Emission, Nutrient Digestibility and Immune Status of Adult Male Goats.","authors":"Neelam Kumari, Anju Kala, Neeta Agarwal, Vishwa Bandhu Chaturvedi, Kennady Vijayalakshmy, Lal Chandra Chaudhary","doi":"10.1111/jpn.14105","DOIUrl":"https://doi.org/10.1111/jpn.14105","url":null,"abstract":"<p><p>Ruminal methanogenesis reduces animal feed utilizing efficiency and is a potent greenhouse gas contributing to climate change. The present study aimed to study the effect of plant secondary metabolites on methane emission, nutrient digestibility and immune status of adult male goats. To fulfil the goal, 20 adult male goats (2.5-3 years of age, body weight [BW] 25.90 ± 1.46 kg) were divided into four groups of five animals. The control was fed a basal diet containing wheat straw and concentrate in a 50:50 ratio without additives. In groups T1, T2 and T3, the basal diet was supplemented with ajwain (Trachyspermum ammi) oil @ 0.06% dry matter intake (DMI), bahera (Terminalia belirica) @ 0.8% DMI and combination of ajwain oil and bahera @ 0.06% and 0.8% DMI, respectively. The DMI, nutrient digestibility, nitrogen intake, outgo and nitrogen balance were similar (p > 0.05) in all four groups. Methane emissions (g/kg DDMI) reduced (p < 0.05) by 22.16%, 23.88% and 33.49% in T1, T2 and T3 compared to control. Both cell-mediated immunity and humoral immune response significantly improved in T3 than other supplemented groups and control. In conclusion, the combination of behera and ajwain oil effectively reduced methane emissions and improved the immune status of adult male goats without affecting nutrient digestibility.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential Oil of Bay Laurel (Laurus nobilis) Enhances Growth and Immunity in Cold-Stressed Nile Tilapia (Oreochromis Niloticus).","authors":"Akram Ismael Shehata, Shimaa A Shahin, Sara Ahmed Taha, Ayaat M Elmaghraby, Mayada Alhoshy, Ali A Soliman, Asem A Amer, Amany M Hendy, Mahmoud S Gewaily, Islam I Teiba, Mohammed F El Basuini","doi":"10.1111/jpn.14107","DOIUrl":"https://doi.org/10.1111/jpn.14107","url":null,"abstract":"<p><p>Bay laurel (Laurus nobilis) essential oil is known for its antimicrobial, anti-inflammatory, and antioxidant properties. This study examined the effects of L. nobilis oil (LN) on Nile tilapia (Oreochromis niloticus) under cold stress conditions (16°C). Tilapia (initial weight, 5.02 ± 0.02 g) were acclimatized to 16°C for 14 days before being fed diets containing 0, 0.5, 1.0, 1.5, 2.0, and 2.5 g/kg LN oil for 84 days. The 1.5 g/kg LN oil group exhibited the highest final body weight and weight gain (p ≤ 0.05), while survival rates peaked at 1 g/kg. Biometric indices and feed efficiency were significantly enhanced, particularly at 1.5 g/kg (p ≤ 0.05). Histological analysis revealed improved intestinal and hepatic structures in LN-supplemented groups, although mild alterations were observed at 2.0 and 2.5 g/kg. Blood biochemical analysis showed increased total protein and reduced cholesterol in supplemented groups. Immune responses, including serum lysozyme activity and bacterial inhibition, were significantly enhanced at 1.5 g/kg or higher (p ≤ 0.05). Antioxidant enzyme activities, including superoxide dismutase (SOD) and catalase (CAT), increased (p ≤ 0.05) with LN oil supplementation, while malondialdehyde (MDA) levels decreased, indicating reduced oxidative stress. Gene expression analysis demonstrated increased insulin-like growth factor 1 and glucose transporter 4 levels with 1.5 g/kg LN oil, and tumor necrosis factor-alpha expression decreased at higher dosages. Dietary LN oil, particularly at 1.5 g/kg, enhances growth, immunity, and antioxidant defense in Nile tilapia under cold stress. Future studies should optimize dosages and explore broader applications across species and conditions.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermoregulatory Response, Growth Performance and Organ Weights of FUNAAB-Alpha Chicken Genotypes Administered Sweet Citrus Peel Powder.","authors":"Bukola Christiana Majekodunmi, Mathew Wheto, Oyegunle Emmanuel Oke, Obafemi Foluso Akinjute, Henry Temitope Ojoawo, Chinedu Ruth Chika, Oluwatobiloba Motunrayo Adekunle, Yusuf Oluwatobiloba Adeyeri, Monsuru Oladimeji Abioja","doi":"10.1111/jpn.14106","DOIUrl":"https://doi.org/10.1111/jpn.14106","url":null,"abstract":"<p><p>Thermoregulatory response and growth performance of FUNAAB-Alpha chickens administered sweet citrus peel powder (SCPP) were investigated in a twelve weeks experiment. A total of 360 1-day-old FUNAAB Alpha chicks were used for the study, comprising 120 birds per genotype: Normal feather (NF), Naked neck (NN) and Frizzle feather (FF). Birds were randomly allotted into 12 treatment groups: NF: 0 g, 3 g, 6 g and 9 g of SCPP, NN: 0 g, 3 g, 6 g and 9 g of SCPP, FF: 0 g, 3 g, 6 g and 9 g of SCPP. Each treatment was replicated thrice with 10 birds per replicate. No (p > 0.05) interactive and main effect of genotype and dosage of SCPP was observed on the Rectal temperature, Skin temperatures under wing and of the breast among the treatments. Similar (p > 0.05) interactive effect was observed on the respiratory rate (RR), however, higher (p < 0.001) RR was recorded in NN and FF compared with NF. Birds in the control group had the highest (p < 0.005) RR compared with birds administered 3 g and 9 g of SCPP/L of water. Interaction and main effect of SCPP dosage on heterophil (H), lymphocyte (L) and heterophil lymphocyte ratio (H/L) were not significant (p > 0.05) across the treatment groups. However, genotype significantly (p < 0.000) influenced H, L and H/L. The FF and NN had significantly (p < 0.000) lower H/L compared with the NF. Higher (p < 0.000) weight gain (WG) was observed in FF and NN compared to NF chickens. Significantly (p < 0.020) lower FCR was observed in 6 g/L (2.26), 3 g/L (2.65) and 9 g/L (2.66) of FF compared with NF on 9 g/L (4.03). Dosage of SCPP significantly (p < 0.001) influenced the relative weights of the intestine. In conclusion, lower H/L, higher WG and better FCR were observed in the NN and FF genotypes with the best FCR recorded at 6 g SCPP/L of water.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D₃ Is Not a Limiting Nutrient Regarding Growth Performance and Tibia Parameters in the Rearing Period of Laying Hens Bred for High Laying Performance Compared to Non-Selected Resource Populations.","authors":"Mareike Kölln, Jana Frahm, Ingrid Halle, Liane Hüther, Jeannette Kluess, Henrieke Meyer-Sievers, Lars Schrader, Steffen Weigend, Sven Dänicke","doi":"10.1111/jpn.14104","DOIUrl":"https://doi.org/10.1111/jpn.14104","url":null,"abstract":"<p><p>Bone damages in laying hens are of great concern in poultry farming. Besides various risk factors like housing systems or nutrient supply during egg production, it has often been hypothesized that genetically high-performing laying hens may be more prone to bone damages. The relevance of dietary support during the rearing period of pullets for optimal bone development has been little addressed so far. In the present study, an increasing dietary vitamin D₃ content within EU legislation was tested during the first 12 weeks of life in two high and two moderate-performing pullet lines (white and brown layer lines). For this purpose, a total of 940 chickens of both sexes were housed at the Institute of Animal Welfare and Animal Husbandry (Friedrich-Loeffler-Institut, Germany). The three experimental diets differed only regarding the added vitamin D₃ amount (300/1000/3000 IU Cholecalciferol/kg diet). After every 4 weeks, randomly chosen animals per genotype and dietary treatment were slaughtered for dissection. Serum 25(OH)-Vitamin D₃ concentrations reflected the dietary treatment. Body weight differed regarding genotype. No effect of dietary vitamin D₃ content as a single influence factor on bone parameters like breaking strength, bone dimensions or mineral content could be shown, but age, sex and genotype had impacts and influenced traits in an interactive manner. Therefore, during the first 12 weeks of the rearing period of layer pullets, the different dietary vitamin D₃ contents did not influence performance or bone parameters in the four genetically diverse purebred layer lines. Adjusted dietary vitamin D₃ recommendations for pullets depending on genetically predetermined egg-laying performance do not appear to be necessary if dietary vitamin D₃ contents are within EU legislation.</p>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effect of Bacteriophages and Organic Acids as a Feed Additive to Reduce Salmonella enteritidis in Challenged Chickens","authors":"Hassan Saleh,&nbsp;Mohammad Taher Mirakzehi,&nbsp;Hosin Moradi bidokhti,&nbsp;Mohsen Kazemi","doi":"10.1111/jpn.14103","DOIUrl":"10.1111/jpn.14103","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to compare the effects of dietary supplementation of bacteriophage (BP) and acidifiers on performance, meat quality, morphology, and intestinal microbiota in chickens challenged and unchallenged with Salmonella enteritidis (SE) and also to investigate the possibility of replacing them in the diet with antibiotics. A total of 1760 male Ross (308) chicks were randomly assigned to 11 dietary treatments (8 pens/with 20 male chickens in each). Dietary treatments were as follows: SE-uninfected (negative control (NC), a basal diet without supplemention; NC+ 500 g/t BP (NBP1); NC+ 1000 g/t BP (NBP2); NC+ 300 mg/kg acidifier A (NAA); NC+ 300 mg/kg acidifier B (NAB)) and SE-infected (positive control (PC), a basal diet without supplemention; PC+ 40 mg/kg Antibiotic enrofloxacin (PA); PC+ 500 g/t BP (PBP1); PC+ 1000 g/t BP (PBP2); PC+ 3000 mg/kg acidifier A (PAA); PC+ 3000 mg/kg acidifier B (PAB)). At 13 D birds in challenged groups were orally gavaged with 1 mL bacterial suspension containing approximately 108 CFU Salmonella enterica. The results indicated that chicks challenged with SE impaired performance so that BWG and FI significantly decreased and FCR increased (<i>p</i> &lt; 0.05). Diets containing a high dose of BP increased BW and improved FCR in challenged and unchallenged chickens. The quality of breast meat showed a decline in oxidation in chickens challenged with SE (<i>p</i> &lt; 0.05). The inclusion of bacteriophage in the diet of chickens (1000 g/t) improved the L*, b*, and oxidation of meat. The use of bacteriophage and acidifier A in the diet significantly increased the Lactobacillus, and LAB count, especially in the challenged groups. The challenge of chickens with SE decreased the villus height and crypt depth in different parts of the small intestine (<i>p</i> &lt; 0.05). The results of the present study suggested that 1000 g/t of BP probably improves chicken performance by increasing beneficial bacteria and decreasing pathogenic bacteria. Also, bacteriophage showed an improvement in the performance reduction of chickens with <i>Salmonella</i> infection. In addition, improved meat quality and beneficial changes in the microbiome and intestinal morphology indicate the effectiveness of BP as an alternative antibiotic growth promoter in broiler diets.</p></div>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":"109 3","pages":"881-890"},"PeriodicalIF":2.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}