Journal of Advances in Microbiology最新文献

{"title":"Mycotoxin Content of Fungi-Contaminated Sorghum Sold in Selected Markets in Ibadan, Nigeria","authors":"B. Bamkefa, O. A. Fatoki, O. Abiola-Olagunju, A. Duyilemi-Olaniyi, A. Abeeb, K. O. Adediran, O. O. Ajogbeje","doi":"10.9734/jamb/2023/v23i7735","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i7735","url":null,"abstract":"Aim: This study sought to evaluate the mycoflora associated with sorghum retailed in some selected markets in Ibadan metropolis and assessing the risks associated with their consumption. \u0000Methodology: Sorghum samples were obtained from Sango, Oja Oba, Ojoo, Bodija and Orita markets in Ibadan, Nigeria. Associated fungi were isolated by direct plating of the sample on Potato Dextrose Agar while they were identified by morphological and microscopic methods. Proximate and aflatoxin content of the samples were determined using standard methods.  \u0000Results: The presence of Aspergillus flavus, A. niger, Mucor sp, Penicillium sp, Trichothecium sp, Curvularia sp., Fusarium monoliforme, Alternaria alternata, and P. roqueforti were observed in the samples. Among these, A. flavus and A. niger were the most predominant with occurrence of 34.92% and 23.81% respectively. The aflatoxin analysis showed a concentration ranging from 46 – 102ppb and was highest in samples collected from Ojoo market. Aflatoxin contamination was not detected in sixty percent of the samples collected while samples from Oja Oba and Bodija markets had the most samples contaminated by aflatoxins. \u0000Conclusion: The presence of fungi and aflatoxin was confirmed in some of the samples collected from major markets in Ibadan. This suggests the need for continuous surveillance and the recommendation of measures that will prevent fungal infestation.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"124 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87868845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Bacteriocin Extracted from Lactobacillus rhamnosus on Cutaneous Wound Healing in Mice","authors":"F. O. Nwajiobi, C. Anyamene, C. Abana","doi":"10.9734/jamb/2023/v23i7734","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i7734","url":null,"abstract":"Aim: This study evaluates the effects of bacteriocin produced by Lactobacillus rhamnosus on cutaneous wound healing in mice. \u0000Study Design: Randomized experimental design was employed in the study. \u0000Methodology: Probiotic L. rhamnosus was isolated from nono milk using MRS agar media and identified based on morphological and biochemical characteristics. The potent bacterium was subjected to bacteriocin production. Antimicrobial activity of the bacteriocin was carried out against wound pathogenic bacteria and wound healing effects of the bacteriocins were assessed on mice. 2 mm cutaneous wound was induced on the mice and crude bacteriocin was topically applied on the wounds. White blood cell count was carried out after days 3, 5, 9, and 14. Histopathological analyses were performed after day 14. \u0000Results: Crude bacteriocin was effective against wound pathogenic bacteria such as S. aureus, P. aeruginosa and E. coli. Topical application of bacteriocin showed a faster and better wound healing when compared to positive control groups in P. aeruginosa infected wound model. Histology examination revealed an increase wound healing time in L. rhamnosus bacteriocin treated mice. The crude bacteriocins demonstrated excellent wound-healing activity in skin excision albino mice model. \u0000Conclusion: L. rhamnosus bacteriocin had various beneficial actions on wounds. It improved wound healing, inhibited wound pathogens and increase wound healing time.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74724734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of Foodborne Bacteria with Outbreak Potentials and Fungi Associated with Tomato (Solanum lycopersicum L.) in Postharvest","authors":"Oscar Akotowanou, E. S. Adjou, S. Kougblénou, Eliane Akpo, Adéyèmi Berane Olubi, E. Ahoussi, A. Yessoufou, H. Bankolé, D. C. Sohounhloué","doi":"10.9734/jamb/2023/v23i7732","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i7732","url":null,"abstract":"The high-water content of tomato predisposes it to spoilage by bacteria and fungi that can pose significant health threats to consumers. However, parameters such as environment and human factors influence the rate of contamination and microbial species involved. Hence, the present study aims to determine the various pathogenic bacteria and fungi associated with tomatoes sold in some markets in southern Benin. A total of 70 tomatoes were sampled, out of which 35 undamaged tomatoes and 35 spoilt tomatoes were processed for examination of bacterial and fungi contamination by standard culture technique following standard protocols. Results indicated that out of the 70 tomatoes samples analyzed, 85.71% are contaminated with enteric bacteria. Escherichia coli was the commonest bacterial contaminant. Foodborne bacteria with outbreak potentials were also detected in analyzed samples. Fungi isolated belong to genera of Aspergillus, Fusarium and Mucor. The most identified species are Aspergillus ochraceus (98.91%), Fusarium oxysporum (84.28%), Aspergillus niger (72.85%) and Aspergillus versicolor (35.71%). The presence of these foodborne pathogens raises concern on public health risks associated with the consumption of fresh tomatoes. Efforts should be made to advise farmers to avoid the use of contaminated wastewater for irrigation during cultivation, and discourage purchasing spoilt tomatoes.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75349089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Antibiotic Sensitivity of Klebsiella pneumoniae among Urinary Tract Infected Patients in Dalhatu Araf Specialist Hospital Lafia, Nasarawa State Nigeria","authors":"I. G. Innocent, A. G. Gowon, C. Ademah, J. B. Agbese, M. I. Kuleve, O. I. Jonah","doi":"10.9734/jamb/2023/v23i7731","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i7731","url":null,"abstract":"Background and Objectives: Klebsiella pneumoniae complications with limited therapeutic options is considered as one major health issues concerning healthcare professionals worldwide. It is one of the leading nosocomial bacterial pathogen that causes most urinary tract infection, and recently the antibiotic resistance of Klebsiella pneumonia has totally increased dramatically. Cross-sectional study was performed from September 2021 to the end of December 2021. This aimed at the isolation and antibiotic sensitivity of the Klebsiella pneumoniae among patients with signs and symptoms of UTI. \u0000Materials and Methods: Cross- sectional study was performed from September 2021 to the end of December 2021. About120 urine specimens collected were analysed to detect the presence of Klebsiella pneumoniae. The Disc diffusion method was used to determine the antibiotic sensitivity pattern of the isolates. \u0000Results: Of the 120 specimens collected, only 72 (60%) produced growth. The sensitivity test revealed that all the isolates were sensitive to levofloxacin, 30 isolates (14.2%) were sensitive to ciprofloxacin, 28 isolates (13.2%) were sensitive to gentamycin, 20 isolates (9.4%) were sensitive to travid, while Augmentin has the sensitivity of 17 (8.1%). It revealed that levofloxacin has the lowest resistance of 0 (0%) while travid has the highest resistance of 15(21.7%). \u0000Conclusion: The antibiotic sensitivity pattern of the isolate revealed that all the isolates were completely sensitive to levofloxacin and more resistant to Augmentin and travid.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77485462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Synthesis of Fe-NPs by Bacteria/Biosurfactant, Kinetic Parameter and Reduction of Cr (VI) from Pollutant Samples","authors":"T. H. Swapna, P. N. Kumar, Yahya Khan, G. Reddy, H. Bee","doi":"10.9734/jamb/2023/v23i6730","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i6730","url":null,"abstract":"Hexavalent Cr is most toxic metal and hazardous compound worldwide. It is also considered to be cellular oxidizing pollutant causing damage, mutagenic and hematopoietic effects to living systems. Present work is focused on synthesis of zero valent iron nano particles (Fe-NPs) using Bacillus subtilis SHB 13, Achromobacter xylosoxidans SHB 204 and Surfactin and their application for Cr (VI) detoxification (100 ppm) in synthetic solutions and polluted sludge, sewage and soil samples about 90-100% reduction observed with above particles. Qualitative analysis, structural characterization of Fe-NPs, adsorption, and recovery of Cr (VI) were carried out using XRD, SEM and TEM. This work explains adsorption isotherm of Cr with iron nano particles (Fe- NPs), was best fitted with Langmuir model and it followed first order rate kinetic reaction. Microcosm studies were carried out using Fe-NPs in sludge, sewage and tannery samples and Cr (VI) was completely (100 %) removed within 72 h. In another microcosm experiment, bacteria were able to reduce (60-70 %) Cr (VI) from soils with and without moisture and glucose. In green house experiments both the bacteria were able to reduce Cr (VI) (65-90%) effectively from both polluted and non-polluted soil and there was increase in root length and shoot length of plant L. esculentum was observed and maximum plant biomass was observed with plants inoculated with SHB 204 and SHB 13.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87239585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro and In-vivo Anti-Candidal Effect of Cnidoscolus aconitifolius Leaves Extracts","authors":"J. C. Adindu, C. Anyamene, C. Ezebialu, D. C. Chukwukaelo, C. F. Chude, L. E. Eruala, A. Umeofor","doi":"10.9734/jamb/2023/v23i6729","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i6729","url":null,"abstract":"Candidiasis is an opportunistic infection caused by pathogens of the genus Candida. Due to the increase in antifungal resistance by Candida species, there is a need for alternatives in its treatment. Cnidoscolus aconitifolius is a medicinal plant with vast nutritional and antimicrobial properties. This study evaluated the in-vitro and in-vivo anti-candidal effect of Cnidoscolus aconitifolius (spinach tree) leaves extract. Clinical isolates of Candida albicans, Candida krusei, Candida tropicalis, Candida dublinensis, Candida glabrata, and Candida parapsilosis were used in this study and Cold maceration technique was employed in the plant extraction process, to obtain its ethanolic, methanolic, and aqueous extract. The agar well diffusion method and 2-fold dilution process -to obtain 250 mg/ml to 15.625mg/ml of the extracts -were used for the antimicrobial sensitivity test and three weeks old female Wistar rats infected with 20µl of standardized C. albicans were used for the in-vivo evaluation. Statistical analysis was done using Statistical package for Social Sciences (SPSS) version 22. In-vitro assay revealed ethanolic extract as the most potent extract with the highest inhibition zone diameter of 12.67±1.15c, 11.67±1.15c, and 12.33±0.58c at 250mg/ml against C. tropicalis, C. krusei2, and C. parapsilosis respectively. C. parapsilosis was the most sensitive to all the extracts. In-vivo, the disappearance of disease symptoms and progressive decrease in rats Candida burden- from 6.35×103 CFU/ml obtained after infection to 2.15×103CFU/ml after treatment revealed the ability of the plant to treat candidiasis. The study suggests that extract of Cnidoscolus aconitifolius leaves could act individually or synergistically as effective drug candidates in the treatment of candidiasis; thus if its individual antifungal constituents are purified, this plant could be the next major anti-candidal agent.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86944964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Aqueous Extract of Moringa oleifera Leaves using GC-MS Analysis","authors":"I. I. Anekwe, Chikwendu Chinwe Ifenyinwa, E. Amadi, Nwogwugwu Ngozi Ursulla, Ihenetu Francis Chukwuebuka","doi":"10.9734/jamb/2023/v23i6727","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i6727","url":null,"abstract":"Background: The Evaluation of plants for its bioactive compounds is of great importance to researchers because of their therapeutic properties which can be harnessed to serve as alternatives to antibiotics or the development of novel drugs to fight human infectious diseases. Moringa oleifera is a member of the Moringaceae family with nutritional and medicinal properties. \u0000Aim: To identify the bioactive constituents present in Moringa oleifera leaves using Gas chromatography-Mass spectrometry. \u0000Methodology: The bioactive compounds were extracted using the Soxhlet method of extraction and was analyzed using Gas chromatography mass-spectrometry. \u0000Results: The chromatogram of the GC-MS analysis used to identify the bioactive constituents present in the aqueous extract of Moringa oleifera leaves extract showed the presence of seventy-eight compounds. This includes 1H-Indene, 2-pyridinyl–methyl ester (9.82%), Anthracene, 9-(2-propenyl) (6.72%), 2,6- Lutidine 3,5- dichloro-4-dideiylthio (5.67%) and various other compounds which were identified as low level. \u0000Conclusions: The GC-MS analysis of Moringa oleifera showed the presence of bioactive compounds. These compounds are of high importance because they can be used in the production of new antimicrobials due to their valuable medicinal and therapeutic properties.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88844108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced L-lysine Production by UV-irradiated and S-2-aminoethyl-L-cysteine Resistant Mutants Derived from Bacillus species Using Agricultural Products as Carbon and Nitrogen Sources","authors":"J. Okpalla, I. A. Ekwealor, E. Okoye, L. C. Okoye, V. E. Ike","doi":"10.9734/jamb/2023/v23i6728","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i6728","url":null,"abstract":"L-Lysine is essential for human and animal nutrition and may be added to food and feed materials to improve the protein quality. It is utilized in human medicine, in cosmetics and in the pharmaceutical industry, particularly in the formulation of diets with balanced amino acid concentration and in amino acid infusions. Enhanced L-lysine production by UV irradiated and S-2-aminoethyl-L-cysteine resistant (AECR) mutants derived from Bacillus species, using agricultural products as carbon and nitrogen sources was studied. The L-lysine producing bacteria had already been isolated from Nigerian soil. They were purified and identified as B. subtilis PR13, B. subtilis PR9 and B. pumilus SS16, using cultural and biochemical characteristics. UV irradiated mutants were obtained by exposing the parent strains which include B. subtilis PR13, B. subtilis PR9 and B. pumilus SS16,  to UV light while, AECR mutants were derived by exposing the parent strains to various concentrations of AEC. The mutants were screened for L-lysine production by cultivating in fermentation media. The results obtained showed that, UV mutants PRMU-8 and PRMU-14 derived from Bacillus subtilis PR13 accumulated higher lysine yield of 2.47 and 2.55 mg/ml compared to the wild type. Also, UV mutant MUPR-4 derived from Bacillus subtilis PR9 recorded a higher lysine yield of 1.77 mg/ml while, enhanced lysine yields of 1.96 and 2.34 mg/ml were observed in UV mutants SSMU-4 and SSMU-9 respectively. AECR mutant PRT-2 derived from Bacillus subtilis PR13, accumulated higher lysine yield of 2.35 mg/ml compared to the wild type. Enhanced  lysine production of 1.47 and 1.32 mg/ml was observed in mutants ART-5 and ART-8 derived from B. subtilis PR9 while, mutant SRT-4 derived from B. pumilus SS16 recorded enhanced lysine accumulation of 2.18 mg/ml. The results obtained in the study showed that L-lysine production by some UV irradiated and AECR mutants was enhanced.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91001674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility of Candida albicans, Staphylococcus aureus and Escherichia coli to Extracts of Mango (Magnifera indica)","authors":"R. Nrior, C. J. Ugboma, Queen Lugbe, D. Ogbonna","doi":"10.9734/jamb/2023/v23i5725","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i5725","url":null,"abstract":"Aim: This study was carried out to investigate the Susceptibility of Candida albicans, Staphylococcus aureus and Escherichia coli to extracts from young and mature mango (Magnifera indica) leaves and stem-bark of the same plant.                                                                                \u0000Study Design: The study employed statistical analysis of the data and interpretation.                                      \u0000Place and Duration of Study: Young and mature mango leaves and stem-barks were collected from the Botanical Garden, Kenule Beeson Saro-Wiwa Polytechnic, Bori, Nigeria, and taken to the laboratory for analyses.                            \u0000Methodology: The samples were dried in an oven at 80oC for 3 days. Thereafter, 50 g of each ground mango leaves and stem-bark (young and mature of the same plant) were soaked separately in 500 ml of water, ethanol (95% v/v), and acetic acid (99.9% v/v) for another 3 days. The soaked materials were filtered through Whatman No. 1 filter paper into sterile beakers and evaporated to dryness in a water-bath at 80oC. The dried extracts obtained were reconstituted with water at concentrations of 100, 75, 50 and 25 mg/ml. Test organisms, Candida albicans, Staphylococcus aureus and Escherichia coli were obtained after proper laboratory screening of isolates from the diagnostic laboratory of the Rivers State University Teaching Hospital, Port Harcourt, Nigeria, for confirmation of identity and storage in universal bottles in a refrigerator. Sensitivity tests were carried out with the agar well diffusion method against the test organisms, using tetracycline as standard control drug (for bacteria) and fluconazole (for Candida), with cultures incubated accordingly. The measured zones of inhibition were compared with the controls and interpreted as resistant, intermediate, or susceptible to mango extracts in accordance with the interpretive guidelines published by the National Committee for Clinical Laboratory Standards (NCCLS). Assays for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also carried out. \u0000Results: Results obtained showed that Escherichia coli was completely susceptible to acetic acid young leaf and young bark extracts at 100 mg/ml concentrations. Staphylococcus aureus was susceptible only to Acetic acid young leaf extract at 100 mg/ml. For Candida albicans complete susceptibility was with acetic acid young bark at 100 mg/ml.  mature leaf extract (100 mg/ml ), acetic acid young bark extract (100 to 50 mg/ml ), aqueous young bark extract (100 mg/ml) and acetic acid mature   Candida albicans was susceptible to acetic acid young and mature bark extract at 100 mg/ml concentration. Minimum inhibitory concentration (MIC) values of acetic acid young leaf extract for all three organisms were 12.5 mg/ml. MIC of ethanolic young leaf extract for E. coli was 12.5 mg/ml whereas that for C. albicans was 50 mg/ml.  Minimum bacteriocidal concentration values wer","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80558069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus Species Isolated from Abattoir in Obio/Akpor and Eleme Local Government Areas of Rivers State","authors":"O. Aleruchi, Hope Barine Deidei, D. Lawson, L. Nnodim, Solomon Ikechi Ogbonna, Gote Yirabari Igoni","doi":"10.9734/jamb/2023/v23i6726","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i6726","url":null,"abstract":"Abattoir operation could be very beneficial to man; in that it provides meat for human consumption and other by-products. The study was aimed at isolation of Staphylococcus species from various equipment used in abattoir. A total of 100 samples were collected from five different surfaces; bucket swab, butchers hand swab, table swab, knife swab and meat swab from four abattoirs; two from Obio/Akpor and two from Eleme Local Government of Rivers State. The samples were transported aseptically to laboratory for immediate analysis. The samples were cultured using standard microbial method on Mannitol salt agar. The isolates of Staphylococcus species were identified using biochemical tests. The Staphylococcal counts ranged from 10.64±0.07 to 25.50±0.71×105 CFU/g for bucket. The least count for the bucket sample was observed in Akpajo while the highest count was observed in Rumuokurushi. For the meat samples the counts ranged between 8.40±0.14 to 12.85±0.07 ×105 CFU/g, the least count was observed in Rumuokoro, while the highest was observed in Aleto. For the hand, knife and table samples the least counts of 10.35±0.35 ×105 CFU/g, 12.50±0.14 ×105 CFU/g and 14.80±0.14 ×105CFU/g were observed from Akpajo, and Rumuokoro, respectively, while the highest count was obtained from hand (26.75±0.07 ×105CFU/g), knife (15.30±0.14x105CFU/g) and table (22.50 ± 0.71x105 CFU/g) in Aleto, Rumuokrushi and Akpajo, respectively. A total of 43 isolates of Staphylococcus species was obtained in the study of which least percentage occurrence of 2.33% was record from table in Akpajo while the highest percentage occurrence of 9.3% was recorded from bucket in Akpajor. The results shows that cross-contamination of meat sold in the abattoir could come from the equipment used in the abattoir hence, their hygienic condition should be ensured.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90190630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}