Journal of Advances in Microbiology最新文献

{"title":"The Influence of Vitamins, Antibiotics and Growth Stimulators on L-lysine Production by Bacillus subtilis Using Agricultural Products as Carbon and Nitrogen Sources","authors":"J. Okpalla, I. A. Ekwealor","doi":"10.9734/jamb/2023/v23i5724","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i5724","url":null,"abstract":"L-Lysine is one of the 9 amino acids which are essential for human and animal nutrition. It is a basic building block of all proteins and cannot be synthesized biologically in the body. The influence of  vitamins, antibiotics and growth stimulators on L-lysine production by Bacillus subtilis using agricultural products as carbon and nitrogen sources was examined. The L-lysine producing bacteria had already been isolated from Nigerian soil. They were purified and Identified as B. subtilis PR13 and B. subtilis PR9, using cultural and biochemical characteristics. The of  influence of vitamins, antibiotics and growth stimulators on L-lysine production by Bacillus subtilis PR13 and 9 was evaluated in 100 ml flasks containing 20 ml fermentation media (FM1 and FM2). The results obtained showed that, an enhanced lysine yield of 3.41 and 1.57 mg/ml by Bacillus subtilis PR13 and Bacillus subtilis PR9, was observed in the presence of 1 µg/ml  of biotin and  10 µg/ml respectively. The supplementation of 0.04 units/ml of penicillin, enhanced optimum L-lysine yield of  2.38 mg/ml for B. subtilis PR 13 and 1.64 mg/ml for B. subtilis PR9 respectively. The addition of  0.1% w/v peptone and yeast extract, enhanced optimum L-lysine yield of  2.66 mg/ml for B. subtilis PR 13 and 1.72 mg/ml for B. subtilis PR9 respectively. There was a positive correlation between peptone and L-lysine production by  B. subtilis PR13 (r= 0.85) and yeast extract and L- lysine production by B. subtilis PR9 (r= 0.54). The results obtained in the study showed that the supplementation of the fermentation media with  some vitamins, antibiotics and growth stimulators enhanced the L-lysine yield of  B. subtilis PR13 and  B. subtilis PR9.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85353185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic Susceptibility Screening of Pathogenic Bacteria Isolated from Locally Produced Soybean (Glycine max) Mlk Sold in Port Harcourt, Nigeria","authors":"Okwelle Austin A., Osakuade Felicia O.","doi":"10.9734/jamb/2023/v23i5723","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i5723","url":null,"abstract":"Antibiotic susceptibility of pathogenic bacteria isolated from locally produced soymilk hawked in Rumuolumeni, Port Harcourt was carried out. Three samples of soymilk were obtained from Rumuokoro Market, Rumuolumeni Market and Choba Markets. Standard microbiological techniques including the pour plate method and relevant biochemical tests were used to isolate, characterize and identify the bacteria cultures. The result of the bacteria count was high in Rumuokoro market 4.2x103 cfu/ml and lowest in Rumuolumeni market 3.4x103cfu/ml. The probable bacteria isolates identified are Escherichia coli, Staphylococcus aureus, Klebseilla pneumonia, Salmonella spp and Streptococcus faecalis. E. coll had the highest percentage occurrence (38.18%) and klebsiella pneumonia the lowest (7.27%). The antibiotic screening showed that the gram-positive isolates S. aureus and Stretococcus faecalisare sensitive to Ampiclox (80%), (100%). Among the gram-negative isolates E. coli was most sensitive to Augumentin (71%), Klebsiella pneumonia was sensitive to Ampicilin (15%) Salmonella spp most sensitive to Streptomycin (70%). The result of this research work shows that all soymilk samples are contaminated with different bacterial species. The implication is that the quality of locally produced soymilk must be closely regulated to avoid transmission of infections to customers. It is also critical that local producers are educated on good manufacturing practices.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84239117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Causes of Collective Food Poisoning (CFP) in University Campuses in Senegal Relating to a Lack of Qualification of University Restaurant Staff","authors":"Faye Samba, T. Cheikh, LO Mamadou, Ndiaye Bou, Mbengue Malick, Sow Demba, Diome Toffène, S. Mbacke","doi":"10.9734/jamb/2023/v23i5722","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i5722","url":null,"abstract":"In Senegal, public universities namely: UGB, UCAD, UIT, UADB and UASZ are often the scene of violent mood movements of students following the occurrence of a Collective Food Poisoning (CFP). These diseases are caused by the consumption of dangerous meals usually prepared by unskilled actors. This study aims to identify the shortcomings related to the lack of qualification of those involved in the catering industry and the causes of CFP, by specifically determining their levels: behaviour, qualification, risk in CFP outbreaks, bacteriological contamination of surfaces and food to which students are exposed. To do this, a questionnaire was developed and a team of investigators was formed. From 2012 to 2017, a retrospective survey was conducted. The choice of targets focused on students, restaurateurs, food vendors, residence chefs and medical workers. A system has been set up for the collection and analysis of food samples (processed fish, hot meals, unpasteurized juices and sandwiches) and surfaces (trays and hands of divers and waitresses) at the UCAD ESEA restaurant under aseptic conditions. Data processing was carried out using an Excel spreadsheet and XLStat software. We have respectively in University Restaurants, University Residences, Fast Foods and Outdoor Restaurants: (17.47; 43.24; 22.21 and 17.08), (21.05; 33.05; 14.02 and 31.88) and (65.4; 21.16; 10.96 and 2.48) for the level of actors' behaviour, qualification and percentage risk. The level of contamination of trays, divers, waitresses, processed fish, meals, unpasteurized juice and sandwiches is 50%, 48%, 55%, 74%, 100%, 94% and 91.5% respectively. In the analysis of these results, it can be said that some causes of CFP are related to the lack of qualification of the staff and therefore, the training of these actors is a priority on these university campuses. To ensure safe meals and student safety, highly qualified staff must be recruited and continuously trained in good hygiene practices and HACCP.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75900124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Molecular Identification of Enterobacter cloacae from Pregnant Women with Urinary Tract Infections in Biu, North Eastern Nigeria, Borno State","authors":"Jummai Mbula, M. Abdulsalami, P. Vantsawa, K. Onuh, U. Ozojiofor, Solomon Bolarinwa Ayodele, Ume Austine Okpaga, Kenneth Onuh, I. A. Oyong","doi":"10.9734/jamb/2023/v23i5721","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i5721","url":null,"abstract":"Urinary Tract Infections (UTIs) represent a significant, widespread, and expensive public health issue owing to their high frequency and the associated health challenges on pregnant women. This study aimed to isolate, molecularly identify Enterobacter cloacae strains from pregnant women with Urinary tract infections in some General Hospitals in Biu, Borno State. A total of 250 vaginal swabs from pregnant women who attended Antenatal Care Unit whose age ranges from 15 to 45 years were recruited using a well-designed questionnaire by random sampling technique. Gram-staining, culture, and wet microscopy were employed to check the presence of bacterial pathogens in the samples. Polymerase Chain Reaction was carried out to further validate the results. The degree of relatedness of the organism was determined using NCBI software. The result revealed that 167 representing 66.8% of the total samples studied showed significant bacterial growth. The ascending order of the percentage of the bacterial agents isolated include: Enterobacter cloacae 47 (28.1%), Klebsiella pneumoniae 36 (21.6%), Escherichia coli 23 (13.8%), Proteus mirabillis 19 (11.4%), Staphylococcus epidermis 15 (9.0%), Staphylococcus aureus14 (8.3%) and Pseudomonas aeruginosa 13 (7.8%). The result of the demographic factors showed that 17 (36.2%) of pregnant women infected within the age of 15–20 years had Enterobacter cloacae. Illiterate pregnant women were 19 (40.4%), unemployed pregnant women were 29 (61.7%), those in their third trimester (27-40 weeks) were 24 (51.1%), women who had 7 and above pregnancies 25 (53.2%), symptomatic and asymptomatic pregnant women within the age of 15–20 years 19 (40.4%), and rural dwellers 33 (70.2%). This study also demonstrated culture technique as the best biochemical method for isolation of bacterial agents compared to wet mount and microscopic Gram-staining isolation method. It can be concluded that there is a 66.8% prevalence of Enterobacter cloacae in Biu LGA of Borno State.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88397568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Bacteria Composition in Smoked Fish Processed in Yeji-Pru East District, Ghana","authors":"Dennis Bardoe, Jones Gyabeng, Daniel Hayford, Ilyas Ibrahim","doi":"10.9734/jamb/2023/v23i4720","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i4720","url":null,"abstract":"Background: Fish safety and quality have been a major area of public health concern. This situation was heavily influenced by several factors ranging from the harvesting environment to the dining table. \u0000Aim: This study aimed at assessing the bacteriological quality of smoked fish in three (3) major fish processing communities in the Yeji-Pru East District, Ghana. \u0000Study design: The study was a comparative cross-sectional design to explore bacteria species occurrence and abundance (loads) of smoked fish in Yeji-Pru East District, Ghana. It also was directed towards determining the characteristics of the situation as it existed during the period of the study, from June to December 2017. \u0000Method: A closed-ended structured questionnaire was used to collect sociodemographic data from 20 owners of the fish processing site. Forty-eight smoked fish samples including; Oncorhynchus sp., Clupea harengus, Chrysichthys auratus and Oreochromis niloticus were surface sterilised, rinsed and dried at 45°C for 24 hours. Ten grams of each fish sample were diluted with 10 ml of Buffered Peptone Water.  Further dilution was prepared using 5 ml of the same diluent. The prepared samples were made to settle for isolations and later inoculated on MacConkey Agar, Shigella-Salmonella Agar, and Salt Mannitol Agar. Microbial colonies were then enumerated using the pour plate method. Data collected were analysed using Microsoft Excel, SPSS and Interactive Chi-square Test at a test significance of 5%. \u0000Results: The results showed absence of Salmonella and Shigella in all fish samples studied. However, it revealed the presence of Escherichia coli and Staphylococcus species at a rate of 79.2 (n= 38) and 89.6% (n= 43), respectively. The microbial load for E. coli and Staphylococcus species were community specific. Statistical analysis showed a significant difference (p < 0.05) between the microbial loads in fish samples obtained at each study site. However, there was no spatial variation in the mean microbial abundances of each selected sample (p > 0.05). The bacteria load observed were lower than the permissible level for human consumption authorised by both the Ghana Standards Authority and the International Commission on Microbiological Specifications for Foods. \u0000Conclusion: The study showed that the pre-smoking and post-smoking activities along the fish processing chain could affect the microbial load and diversity. Therefore, the adoption of a good processing practice was highly advocated.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75872044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical and Microbial Characterization of Treated and Untreated Produced Water","authors":"L. Tudararo-Aherobo, A. J. Egieya","doi":"10.9734/jamb/2023/v23i4719","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i4719","url":null,"abstract":"Introduction: The discharge of produced water into the aquatic environment presents a risk to the environment. This is a form of pollution and may release toxicants that are highly noxious to sensitive marine species even at low concentrations, which causes bio-degeneration/transformation and biodiversity loss. \u0000Research Gap: There are insufficient literatures on extensive monitoring of the physicochemical and microbial characteristics of produced water. Existing literatures concerning analysis of treated and untreated produced water are not comprehensive with respect to number of physicochemical variables analyzed. \u0000Aim: To ascertain if the physicochemical properties and microbial population of produced water were within acceptable regulatoy specifications.  \u0000Place and Duration of Study: The study was conducted in the Federal University of Petroleum Resources, Effurun, Delta State, Nigeria from July, 2021 – April, 2022. \u0000Methodology: Electrical conductivity, dissolved oxygen, pH, temperature, turbidity, total petroleum hydrocarbons, nitrates, phosphates, sulphates and others were assessed using standard methods. Microbial counts were carried out for total heterotrophic bacteria and hydrocarbon utilizing bacteria using the pour plate method. \u0000Results: Values obtained for some key physicochemical parameters monitored in the produced water are as follow:  pH (8.10±0.03 for untreated and 8.27±0.01 for treated); electrical conductivity (35700±11 µs/cm for untreated and 41600±17 µs/cm for treated); total dissolved solids (22848±14mg/l for untreated and 26629±9mg/l for treated); dissolved oxygen (2.05±0.01mg/l for untreated and 4.23±0.03mg/l for treated); biochemical oxygen demand (28.90±0.7mg/l for untreated and 18.4±0.1mg/l for treated); phosphate (2.05±0.01mg/l for untreated and 4.23±0.03mg/l for treated); nitrate (54.82±1.9mg/l for untreated and 50.21±0.9mg/l for treated); total hydrocarbon content (118.00±0.00mg/l for untreated and 34.00±0.00mg/l); total heterotrophic bacteria (4.1e+04 CFU/ml for untreated and 2.8e+04 CFU/ml for treated); and hydrocarbon utilizing bacteria (2.44e+03 CFU/ml for untreated and 1.58e+03 CFU/ml for treated). Statistical analysis of the produced water showed varying forms of correlation between physicochemical parameters of untreated and treated produced water. \u0000Conclusion: It is important to assess properties of produced water before disposal into aquatic environment as chronic impact associated with long-term exposures may pose potential ecological risks.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88174628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Escherichia coli Bacteriophages Production","authors":"V. Kola, K. Srikanth, Shaik Muzammil Pasha, Y. S. Goutham, C. Pasha","doi":"10.9734/jamb/2023/v23i4718","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i4718","url":null,"abstract":"Aims: Phage therapy may be the best alternative to antibiotics in poultry. There is a need for optimization of phage production conditions to produce more Escherichia coli specific phages for poultry applications. \u0000Methodology: An E. coli λ bacteriophage isolated in our laboratory was used for optimization studies. An optimization of the E. coli λ bacteriophage production process was carried out by conventional methods to identify the factors, and the Thaguchi process was used for the optimization of conditions for increased bacteriophage production. With four factors each at three levels L9, experimental runs were carried out. \u0000Results: In the conventional method, 8 factors were studied and 4 selected for statistical optimization by Taguchi. At optimized conditions of modified nutrient broth with 1% glycerol, temperature 37oC, 100 rpm for 12 hours of phage multiplication, an improvement of the final titer was achieved from a control 2x109  to optimized 5x1012 Plauque formation Units (PFU)/mL. \u0000Conclusion: Phage production is dependent on bacterial growth and bacteriophage multiplication in bacteria. In such a complicated system, the Thaguchi method will be a convenient method of phage production optimization.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79223228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycoremediation of Heavy Metals from Electronic Waste Polluted Water Using Indigenous Fungi and Its Implications","authors":"O. N. Majolagbe, O. G. Ariyo, O. Bello, E. G. Adeyeni, A. Owoseni, O. O. Oluranti","doi":"10.9734/jamb/2023/v23i4717","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i4717","url":null,"abstract":"Background: Heavy metals (HM) pollutants are crucial environmental and public health problems due to their toxicity, which has implications on public health. The site of study has been reported to be densely polluted with heavy metals as a result of dumping used electronic wastes into the water body. \u0000Aim: This study was carried out to determine the biosorption potentials of indigenous fungi isolates in reducing heavy metal present in electronic wastes polluted water body. \u0000Study Design: This was a laboratory based study. \u0000Place and Duration of Study: The study was conducted at the Microbial Resources Research Laboratory, Department of Pure and Applied Biology, Ladoke Akintola University of Technology Ogbomoso, Nigeria from July 2021 to June 2022. \u0000Methodology: Heavy metal polluted water sample was collected near the dumpsite of e-waste in Alaba International market, Lagos Nigeria. Fungi were isolated from the polluted water sample by carrying out serial dilution. Pure colonies were obtained and stored at 4°C. Media formulations (MF) trials for the biosorption process was achieved using brewery waste and honeycomb extracts. Exactly 200 ml of MF were dispensed into a 500 ml Elmeryer flask containing 100 ml of the e-waste polluted water and fugal discs. Atomic absorption spectrophotometer was used to determine the heavy metal concentration in the water samples. The biological interactions of the fungi with the polluted water sample was monitored using Fourier transform infrared (FTIR) spectroscopy. The pH, electrical conductivity and other physicochemical parameters were also determined. \u0000Results: Trichoderma harzianum, T. viride, Fusarium oxysporum, Aspergillus flavus and A. niger were isolated from the heavy metal polluted water samples. Heavy metal such as Pb (13.30 mg/L), Cd (16.50 mg/L), Cr (6.41 mg/L), Ni (3.81 mg/L), Zn (8.85 mg/L), Cu (8.33 mg/L), Fe (5.60 mg/L) had values which were higher than the acceptable limits. Biosorption efficiency of each of the fungus in reducing the metals present in the sample was in the increasing order of A. niger<A. flavus<F. oxysporum<T. viride<T. harzianum. FTIR showed that some peaks were shifted to lower wavenumber as a result of the interactions of the fungus with the heavy metal in the water sample. This study revealed that T. harzianum had the highest biosorption efficiency for the removal of heavy metal in the polluted water sample. \u0000Conclusion: It is essential that heavy metals be removed from polluted water body to avoid its deleterious effects on public health. This can be achieved biologically using any of the fungi isolated in this work.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75389047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Papilloma Virus (HPV) Infection and Vaccination: Knowledge, Attitudes and Practices among Young Adults Attending Universities in Cameroon","authors":"M. H. Afegenwi, Ayong Shantal Feduma, Bongadzeven Blandine Emilio, Musi Ina Notang","doi":"10.9734/jamb/2023/v23i4716","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i4716","url":null,"abstract":"Aims: This study was initiated to assess the knowledge of human papillomavirus (HPV) infection and vaccination, attitudes towards HPV vaccines, as well as risky sexual practices of young university students towards HPV infection.   \u0000Study Design: This was a cross-sectional descriptive study that included university-going young adults of both sexes, aged 18 to 25 years.   \u0000Place and Duration of Study: The study was conducted in four randomly selected state universities, located in different geographic regions of Cameroon. The study took place between April and July 2022. \u0000Methodology: The survey instrument was a self-administered anonymous questionnaire. Participants were randomly given questionnaires to be completed. Data was imported into SPSS version 23 software for analyses. Participants were categorised into one of two groups - poor level of knowledge and good level of knowledge, depending on the number of correct answers to 7 questions about knowledge of HPV infection and vaccination. Chi-square test and logistic regression were used to examine differences in parameters. \u0000Results: 1263 questionnaires were returned. Most participants (78.9%) were undergraduates aged 20 - 21 years (32.7%). Overall, just 87/1263 (6.9%) of participants had a good knowledge of HPV infection and vaccination, most of whom were females (56.7%). Educational level (OR 3.11, 95% CI (1.24 – 7.85), P = .02), field of study (OR 0.42, 95% CI (0.17 – 1.04), P = .04) and plans to become vaccinated (OR 0.33, 95% CI (0.14 – 0.76), P = .009) were predictors of good knowledge of HPV. Attitudes towards HPV vaccination were overall positive. 71.9% participants were sexually active, most of whom only used condoms sometimes (47.2%). \u0000Conclusion: These findings underscore the need for educational programs and public health policies targeted to this age bracket, to increase students’ awareness of the risks associated with HPV infection as this can potentially increase the vaccination rate in the country.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88027415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Microorganisms from Makeup Brushes in Rivers State University Nigeria and Its Environs","authors":"V. N. Agi, O. A. Ollor, C. Azike, C. V. Onwuasoanya","doi":"10.9734/jamb/2023/v23i3715","DOIUrl":"https://doi.org/10.9734/jamb/2023/v23i3715","url":null,"abstract":"Components of makeups and cosmetic supply may serve as source and medium for the growth of microorganisms. A vast majority of females nowadays apply makeups in order to enhance their facial looks. Repetitive use and sharing of the makeup brushes by different customers may become a potential pathway for microbial pathogens spread. The major goal of this study was to isolate and identify microbial contaminants of makeup brushes from students within Rivers State University and its environs. And also determine the antimicrobial sensitivity of the isolated microorganisms to different antimicrobial agents. Eighty (80) samples of makeup brushes were collected from different hostels, personal houses, and beauty salons and were cultured by inoculating them into different culture media as Nutrient, Blood and Sabouraud Dextrose agar to isolate bacteria and fungi respectively. The identification of isolated bacteria was confirmed by using pure culture of the isolates, Gram stain, and biochemical tests which were catalase, coagulase, and others including germ tube tests. The sensitivity test of the isolated bacteria to different antibiotics was carried out using the Optodisc diffusion method. The microorganisms isolated and identified were Staphylococcus epidermidis (55%), Staphylococcus aureus (22.5%), and Candida albicans (22.5%). The isolated bacteria were susceptible to Ciprofloxacin, Levofloxacin and Gentamycin. The findings of this study have shown that both Staphylococcus epidermidis and Staphylococcus aureus were isolated as bacteria, while Candida albicans as fungi from makeup brushes. Staphylococcus epidermidis are considered as normal skin flora whereas, Staphylococcus aureus could be pathogenic and present as source of infecting bacteria. It could be concluded that it is possible to isolate bacteria and fungi that may be pathogenic from makeup brushes, that may act as source of infecting pathogens therefore increases the risk of infection and public health concern.","PeriodicalId":14918,"journal":{"name":"Journal of Advances in Microbiology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85409467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}