Staphylococcus Species Isolated from Abattoir in Obio/Akpor and Eleme Local Government Areas of Rivers State

O. Aleruchi, Hope Barine Deidei, D. Lawson, L. Nnodim, Solomon Ikechi Ogbonna, Gote Yirabari Igoni
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Abstract

Abattoir operation could be very beneficial to man; in that it provides meat for human consumption and other by-products. The study was aimed at isolation of Staphylococcus species from various equipment used in abattoir. A total of 100 samples were collected from five different surfaces; bucket swab, butchers hand swab, table swab, knife swab and meat swab from four abattoirs; two from Obio/Akpor and two from Eleme Local Government of Rivers State. The samples were transported aseptically to laboratory for immediate analysis. The samples were cultured using standard microbial method on Mannitol salt agar. The isolates of Staphylococcus species were identified using biochemical tests. The Staphylococcal counts ranged from 10.64±0.07 to 25.50±0.71×105 CFU/g for bucket. The least count for the bucket sample was observed in Akpajo while the highest count was observed in Rumuokurushi. For the meat samples the counts ranged between 8.40±0.14 to 12.85±0.07 ×105 CFU/g, the least count was observed in Rumuokoro, while the highest was observed in Aleto. For the hand, knife and table samples the least counts of 10.35±0.35 ×105 CFU/g, 12.50±0.14 ×105 CFU/g and 14.80±0.14 ×105CFU/g were observed from Akpajo, and Rumuokoro, respectively, while the highest count was obtained from hand (26.75±0.07 ×105CFU/g), knife (15.30±0.14x105CFU/g) and table (22.50 ± 0.71x105 CFU/g) in Aleto, Rumuokrushi and Akpajo, respectively. A total of 43 isolates of Staphylococcus species was obtained in the study of which least percentage occurrence of 2.33% was record from table in Akpajo while the highest percentage occurrence of 9.3% was recorded from bucket in Akpajor. The results shows that cross-contamination of meat sold in the abattoir could come from the equipment used in the abattoir hence, their hygienic condition should be ensured.
从河流州Obio/Akpor和Eleme地方政府地区的屠宰场分离的葡萄球菌种类
屠宰场的经营可能对人类非常有益;因为它提供了供人类食用的肉和其他副产品。本研究旨在从屠宰场使用的各种设备中分离葡萄球菌。从五个不同的表面共收集了100个样本;桶拭子、屠夫手拭子、桌子拭子、刀拭子和四个屠宰场的肉拭子;两名来自奥比奥/阿克波尔,两名来自河流州埃莱姆地方政府。样品无菌运输到实验室进行即时分析。采用标准微生物法在甘露醇盐琼脂上进行培养。采用生化试验对分离的葡萄球菌进行鉴定。桶内葡萄球菌计数范围为10.64±0.07 ~ 25.50±0.71×105 CFU/g。在阿克帕霍观察到的桶样数量最少,而在鲁穆库鲁希观察到的桶样数量最多。肉制品中细菌计数范围为8.40±0.14 ~ 12.85±0.07 ×105 CFU/g,鲁木克罗最少,阿莱托最高。手、刀和桌子样品中,阿克帕霍和鲁穆奥科罗的细菌数量最少,分别为10.35±0.35 ×105CFU/g、12.50±0.14 ×105CFU/g和14.80±0.14 ×105CFU/g,而阿勒托、鲁穆奥克鲁什和阿克帕霍的细菌数量最多,分别为手(26.75±0.07 ×105CFU/g)、刀(15.30±0.14 x105cfu /g)和桌子(22.50±0.71x105 CFU/g)。研究共分离出43株葡萄球菌,其中阿克帕约table的发生率最低,为2.33%,而阿克帕约bucket的发生率最高,为9.3%。结果表明,在屠宰场销售的肉类可能受到来自屠宰场使用的设备的交叉污染,因此应确保其卫生状况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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