Journal of applied biomedicine最新文献

{"title":"Elevated plasma levels of cell-free mtDNA are associated with acute rejection following heart transplantation.","authors":"Dana Dlouha, Kristyna Janouskova, Sarka Chytilova, Jevgenija Vymetalova, Marianna Lukasova, Sarka Novakova, Eva Rohlova, Jaroslav A Hubacek","doi":"10.32725/jab.2025.014","DOIUrl":"https://doi.org/10.32725/jab.2025.014","url":null,"abstract":"<p><p>Acute rejection (AR) following heart transplantation (HTx) is a common complication, especially in the early post-HTx period. Mitochondrial DNA (mtDNA), released into circulation from stressed mitochondria, mimics ongoing immune activation and facilitates the release of pro-inflammatory substances. Our study aimed to assess cell-free mtDNA levels to identify early indicators of acute rejection progression. The absolute concentration of cf-mtDNA (cp/μl) was measured in 77 adult patients using quantitative polymerase chain reaction. Blood samples (n = 300) were collected before their corresponding biopsy according to the timeline within the first year post-HTx. The median cf-mtDNA levels in samples with confirmed AR (n = 57) was higher compared to samples without diagnosed rejection (n = 210; Padj < 0.01). When acute cellular (ACR; n = 39) and antibody-mediated rejection (AMR; n = 18) were analyzed separately, only AMR demonstrated higher levels compared to samples without diagnosed rejection (Padj = 0.02). The highest cf-mtDNA levels were detected in samples collected during early post-HTx complications compared to samples without rejection and AR samples (for both Padj < 0.0001). Both ACR and AMR were observed throughout the one-year period, with the majority (3rd quartile) occurring during the first 200 days post-HTx. Post-HTx complications, such as graft dysfunction or acute kidney injury, were observed within the first 11 days, with the majority (71.4%) occurring within 5 days post-HTx. The presence of AR, and specifically AMR, is associated with elevated levels of cf-mtDNA. The increase in plasma cf-mtDNA levels strongly reflects the occurrence of early complications following HTx.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"97-106"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of late postoperative bleeding in OSA-related tonsil surgery: a multicentric retrospective study.","authors":"Jan Vodicka, Martin Chovanec, Milan Urik, Bretislav Gal, Rami Katra, Petr Skopek, Veronika Glumbikova, Anna Svejdova, Zdenek Knizek, Jan Kolin, Hana Dolezalova, Libor Sychra, Patrik Bursa, Lenka Jetmarova, Silvia Berkova, Pavel Strejcek, Josef Hajek, Tomas Kostlivy, David Slouka","doi":"10.32725/jab.2025.016","DOIUrl":"https://doi.org/10.32725/jab.2025.016","url":null,"abstract":"<p><strong>Introduction: </strong>Tonsil-related procedures are considered fundamental and effective in the surgical treatment of obstructive sleep apnea (OSA). The range of techniques includes intratonsillar approaches, such as tonsillotomy (TT), as well as extracapsular procedures, such as tonsillectomy (TE) and uvulopalatopharyngoplasty (UPPP). Patients undergoing these procedures span all age groups, from children to seniors.</p><p><strong>Methods: </strong>This multicentric retrospective study, conducted between 2014 and 2018, analysed data from 3,498 patients who underwent bilateral TT, TE, or UPPP for OSA or ronchopathy. The cohort included 2,221 men (63.49%) and 1,277 women (36.51%). Of these, 2,808 patients (80.27%) underwent TT, 226 (6.46%) underwent TE, and 464 (13.26%) underwent UPPP.</p><p><strong>Results: </strong>Late postoperative haemorrhage (LPOH) occurrence was significantly associated with the type of surgery (p < 0.0001) and the hospital where the procedure was performed (p < 0.0001). The incidence of LPOH in the TT group ranged from 0% to 5.88% across hospitals (p = 0.0068); whereas in the TE and UPPP groups, rates ranged from 0% to 33.33% (p = 0.0413 and p = 0.0409, respectively). The occurrence of repetitive bleeding was not influenced by treatment choice (readmission vs. outpatient care, observation vs. surgical revision, general vs. local anaesthesia). The severity of bleeding in all three groups was not affected by age and gender. The use of anticoagulants negatively impacted LPOH severity (p = 0.0166) in the UPPP group. No deaths occurred in our sample; however, three cases of severe postoperative bleeding (grade \"D\") were observed.</p><p><strong>Conclusion: </strong>Late postoperative haemorrhage remains a serious complication of tonsil-related surgery with the potential for life-threatening outcomes. The marked variability in bleeding incidence between surgical techniques and departments highlights the need for standardised perioperative protocols. Although no fatalities occurred, the occurrence of severe cases underlines the importance of vigilant postoperative monitoring. In our OSA cohort, tonsillotomy showed favourable safety, and recent evidence suggests it may represent a valuable alternative also in recurrent tonsillitis, warranting further research.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"126-137"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum xanthine oxidoreductase and oxidative stress are associated with bladder cancer: a case-control study from Jordan.","authors":"Lina Elsalem, Abrar A Aleikish, Nosayba Al-Azzam, Mahmoud A Alfaqih, Haneen A Basheer, Omar Halalsheh","doi":"10.32725/jab.2025.015","DOIUrl":"https://doi.org/10.32725/jab.2025.015","url":null,"abstract":"<p><p>Xanthine oxidoreductase (XOR) is an oxidant enzyme that exists mainly in two distinct forms: the dehydrogenase form [xanthine dehydrogenase (XDH)] and the oxidized form [xanthine oxidase (XO)]. XO might contribute to tumorigenesis through direct metabolic activation of carcinogens and indirect generation of free radicals. Oxidative stress is one of the leading causes of bladder cancer (BC). Smoking and genetic susceptibility are also linked to oxidative stress and BC. This study investigated the association between XO serum levels and XOR genetic polymorphisms with BC. A case-control study was conducted among 109 BC patients and 109 controls matched by age, gender, body mass index, and smoking status. Serum levels of XO and 8-hydroxy-2'-deoxyguanosine (8-OhdG) were measured using ELISA, while thiobarbituric acid reactive substances (TBARS) and protein carbonyl (PC) were assessed using colorimetric assays. XOR single nucleotide polymorphisms were analyzed via tetra-primer ARMS-PCR. XO levels were significantly higher in BC patients than in controls [(5.11 ± 0.28 vs 3.83 ± 0.23) ng/ml, respectively (p < 0.0006)]. Among smokers, XO levels were also elevated in BC cases compared with controls [(5.29 ± 0.35 vs 3.41 ± 0.28) ng/ml, respectively (p < 0.0001)]. Oxidative stress biomarkers were elevated in BC patients compared with controls: 8-OHdG (19.39 ± 1.37 vs 16.32 ± 1.37 nmol/l), PC (8.88 ± 0.56 vs 4.42 ± 0.56) nmol/mg of protein, and TBARS (4.23 vs 3.15) µmol/ml, respectively (p < 0.05). Haplotype analysis showed that TGTCA, TGTA, TGA, and GTA were more frequent in BC patients and associated with increased BC status [4.17 (1.16-15.00), 1.84 (1.11-3.05), 1.62 (1.01-2.60), and 1.66 (1.02-2.71) fold increase in risk, respectively (p < 0.05)]. Elevated XO and oxidative stress markers are associated with BC, supporting their role in BC pathogenesis. Our findings suggest that they may act as potential diagnostic or therapeutic targets. However, mechanistic studies are required to clarify whether XO/oxidative stress markers contribute directly to carcinogenesis or reflects general redox imbalance in malignancy. Specific XOR haplotypes might serve as biomarkers for BC.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"107-116"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of the antidiabetic drug metformin on the aquatic crustacean Daphnia magna.","authors":"Martina Poncarova, Sarka Klementova, Michal Sorf","doi":"10.32725/jab.2025.011","DOIUrl":"https://doi.org/10.32725/jab.2025.011","url":null,"abstract":"<p><strong>Background: </strong>The antidiabetic drug metformin has been repeatedly detected in surface waters worldwide. This study investigates the effects of the environmentally relevant concentration of metformin on a non-target aquatic organism - a freshwater crustacean, Daphnia magna, with an emphasis on the stress response of daphnids and the long-term effects on their consecutive generations.</p><p><strong>Methods: </strong>The chronic toxicity test and the consecutive generations test were inspired by the OECD method. The total antioxidant capacity (Trolox equivalent - TEAC), superoxide dismutase (SOD) activity, and catalase (CAT) activity were related to the protein content in the tested daphnids.</p><p><strong>Results: </strong>Elevated antioxidant activities were revealed in daphnids exposed to metformin in comparison to the control group (1.9 × for TEAC, 1.7 × for SOD; 1.3 × for CAT). Furthermore, diminished body sizes and malformations in the digestive system, spine and carapace were detected in newborn juveniles in the second and third generations exposed to metformin.</p><p><strong>Conclusion: </strong>Long-term exposure to metformin in environmentally relevant concentrations led to a significant detrimental reaction in aquatic crustaceans.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"144-151"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of healthy sinonasal microbiome - single-centre study in the Czech Republic.","authors":"Kristyna Maminak, Karla Janouskova, Richard Holy, Alzbeta Prasilova, Tomas Filipovsky, Daniel Kovar, Jaromir Astl","doi":"10.32725/jab.2025.012","DOIUrl":"https://doi.org/10.32725/jab.2025.012","url":null,"abstract":"<p><strong>Introduction: </strong>The human nasal cavity and paranasal sinuses host a complex and dynamic microbiome which has a crucial role in mucosal immunity. A comprehensive profile of the healthy sinonasal microbiome remains limited. The purpose of our study was to characterize the healthy sinonasal microbiome in adults using 16S rRNA long-read sequencing to enable species-level resolution, and to assess its associations with demographical and clinical factors such as smoking, allergy history, and olfactory function.</p><p><strong>Study design: </strong>We performed a prospective, single-centre study analysing middle meatus samples from 27 healthy individuals undergoing septoplasty in the age range from 21 to 57 years, excluding those with antibiotic and corticosteroid use and those with signs of acute or chronic rhinosinusitis.</p><p><strong>Results: </strong>A high interindividual variability in the composition of healthy sinonasal microbiome was observed. At the phylum level, it was dominated by Firmicutes (48.96%), Actinobacteria (34.83%), and Proteobacteria (13.85%), while Firmicutes and Actinobacteria were consistently present in all samples. At the genus level, Staphylococcus spp. (32.32%), Cutibacterium (28.04%), and Corynebacterium (4.66%) were most abundant. We observed trend level correlations between phyla and some clinical factors (e.g., smoking and olfactory dysfunction) and selected phyla. However, none remained significant after false discovery rate (FDR) correction across taxa.</p><p><strong>Conclusion: </strong>The study proposes Staphylococcus spp., Corynebacterium spp., and Cutibacterium spp. to be a core taxa in the healthy sinonasal microbiome. Amid the interindividual diversity in our cohort, there was evidence of a stable core microbiome potentially influenced by environmental and host factors. Our findings suggest a baseline reference for distinguishing a dysbiosis in upper respiratory disease.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"117-125"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applying the technology ORBEYE^TM exoscope in transoral exoscopic laryngeal surgery: single centre prospective study.","authors":"Richard Holy, Tomas Filipovsky, Eva Lukavcova, David Kalfert, Pavol Prazenica, Petra Dytrych, Jiri Hlozek, Jan Rotnagl, Daniel Kovar, Jaromir Astl","doi":"10.32725/jab.2025.010","DOIUrl":"https://doi.org/10.32725/jab.2025.010","url":null,"abstract":"<p><strong>Introduction: </strong>ORBEYE^TM exoscope offers superior visualization with clearer imaging compared to standard methods and supports narrow band imaging (NBI). ORBEYE^TM provides better visualization of tissue structures, thus increasing the accuracy of the surgical procedure. The systematic use of ORBEYE^TM in transoral exoscopic laryngeal surgery (TOLES) is rarely documented. This study evaluates the sensitivity and specificity of TOLES using ORBEYE^TM with white light and NBI modalities.</p><p><strong>Methods: </strong>Between 8/2021 and 8/2024, 84 patients underwent TOLES using ORBEYE^TM with white light and NBI modes. All surgeries were performed in a specialized setting with consistent preoperative and intraoperative imaging protocols.</p><p><strong>Results: </strong>TOLES using ORBEYE^TM was successfully performed in all 84 patients. A statistically significant dependence (p < 0.001) was observed between preoperative/perioperative (TOLES) findings and definitive histology for both white light and NBI modalities. ORBEYE^TM facilitated detailed imaging of tissue structures and allowed targeted biopsies.</p><p><strong>Conclusion: </strong>TOLES with perioperative NBI/white light endoscopy achieved a high correlation between pre-histopathological diagnoses and final histopathological results. ORBEYE^TM exoscope provides three-dimensional 4K resolution, superior imaging, and improved ergonomics for the surgeon, reducing workload and increasing efficiency. It delivered more efficient surgical team collaboration and experience sharing. The integration of NBI into the TOLES system facilitated accurate navigation and targeting of the biopsy, helping to establish correct definitive diagnosis. TOLES showed more accurate scoring of perioperative NBI findings.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"138-143"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary metabolites from halotolerant filamentous fungi as potential topical cosmeceutical ingredients.","authors":"Chi Hoang, Ha Tran, Hang Tran, Diep Hoang, Quan Nguyen, Cuong Le","doi":"10.32725/jab.2025.013","DOIUrl":"https://doi.org/10.32725/jab.2025.013","url":null,"abstract":"<p><p>The use of natural products in cosmetics and pharmacy has risen dramatically in recent years, leading to the overexploitation of flora and fauna worldwide and threatening the environmental sustainability. Microbe-derived components could help to solve the problem due to their independently controllable cultural property. To investigate the potential of microfungi for producing potential novel cosmeceuticals, cerevisterol (1), aloesol (2), 3β,5α,9α-trihydroxyergosta-7,22-diene-6-one (3), and ergosterol peroxide (4) were isolated from the halotolerant fungal strains Penicillium brefeldianum CL6 and Talaromyces sp. S3-Rt-N3. They were then tested for biological properties, including anti-microbial, tyrosinase inhibitory, and wound healing activities. The results revealed the wound-healing potentials of two fungal compounds - (1) and (2) - in terms of cell proliferation promotion in NIH-3T3 murine fibroblasts, and the tyrosinase inhibitory potential of fungal compounds (1), (3), and (4) in the substrates L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA). Interestingly, compound (1) exhibited antimicrobial activity against acne-causing bacterium Propionibacterium acnes. These results have revealed new prospects for the application of microorganisms-derived compounds, especially in the cosmetics industry.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 3","pages":"152-162"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the secrets of aortic pseudoaneurysms - exploring tensile testing of prostheses, anastomoses, and native vessels in the thoracic aorta: A clinical-engineering correlation.","authors":"Sandra Recicarova, Hynek Chlup, Michael Jonak, Ivan Netuka","doi":"10.32725/jab.2025.008","DOIUrl":"https://doi.org/10.32725/jab.2025.008","url":null,"abstract":"<p><strong>Introduction: </strong>This study examines the mechanical properties of thoracic aortic false aneurysms (TAFA) and how the use of vascular prostheses, native vessels, and anastomoses affects their development. This is done through tensile testing, simulating a Bentall procedure, which is the most common surgery leading to TAFA development.</p><p><strong>Methods: </strong>We conducted uniaxial tensile tests on the native right and left coronary arteries from five cadaveric donors. They were anastomosed to two vascular prostheses in the longitudinal and circumferential directions to assess their mechanical responses under load.</p><p><strong>Results and discussion: </strong>All anastomosis specimens ruptured on the native vessel side, with no breaches occurring on the prosthesis side. The P2 prosthesis exhibited a mechanical response closer to that of the native vessel compared to the P1 prosthesis. There were no statistically significant differences in wall thickness or mechanical properties between the left and right coronary artery samples, leading to the merging of these groups. The strain of the anastomosis in the longitudinal direction was significantly higher than in the circumferential direction. In both directions, the strain at the onset of rupture was greater than that of the native vessel, with a particularly notable difference in the longitudinal direction. Although there was no significant difference in stress values between the longitudinal and circumferential directions, forces per suture were slightly higher in the circumferential direction.</p><p><strong>Conclusion: </strong>Using the \"endo-button buttress technique\" with a double-layer anastomosis can help distribute the load and reduce stress. An alternative option is to use a Carrel patch to reinforce the connection between the target site and the conduit. Additionally, autologous pericardium can be employed for reinforcement.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 2","pages":"80-90"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of olfactory and trigeminal event-related potentials (OERPs/TERPs) in the assessment of olfactory function in subjects with chronic rhinosinusitis with nasal polyposis.","authors":"Karla Janouskova, Oleksii Vorobiov, Kristyna Maminak, David Kalfert, Libor Vasina, Petra Dytrych, Nikola Pastorkova, Jiri Hlozek, Daniel Kovar, Jan Vodicka, Vaclav Masopust, Jaromir Astl, Richard Holy","doi":"10.32725/jab.2025.006","DOIUrl":"https://doi.org/10.32725/jab.2025.006","url":null,"abstract":"<p><strong>Objectives: </strong>Smell impairment (SI) is a well-known symptom of chronic rhinosinusitis with nasal polyps (CRSwNP). The aim of study was to analyze olfactory and trigeminal event-related potentials (OERPs/TERPs) and psychophysical smell tests in subjects with CRSwNP, and its potential role in clinical practice.</p><p><strong>Methods: </strong>Prospective study included 57 subjects. Two investigated groups with CRSwNP and one control group. Group I (n = 20) contained subjects without CRSwNP before septoplasty. Group II (n = 18) contained subjects with CRSwNP without therapy. Group III (n = 19) contained subjects with CRSwNP after intranasal corticosteroid therapy. Sniffin stick identification smell test and OERPs/TERPs were performed in all subjects.</p><p><strong>Results: </strong>According to the psychophysical smell test, SI was detected in 65% of subjects with CRSwNP. In the control Group I (without CRSwNP), the absence of OERPs was 5.0%, whilethe mean absence rate in Groups II, III (with CRSwNP) was 8.1%. The highest percentage of absence of OERPs was registered in Group II (11.1%). Absence of TERPs was detected in an average of 21.6% of CRSwNP subjects. Group III showed the highest percentage of absence of TERPs (32%). In the control Group I, TERPs were absent in 0% of subjects.</p><p><strong>Conclusion: </strong>CRSwNP significantly impairs olfactory function compared to patients without CRSwNP, Absence of TERPs was detected in an average of 21.6% of CRSwNP subjects versus healthy controls (0%). Presence of TERPs appears to be a predictor of preservation of olfactory function.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 2","pages":"57-62"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine improves atrial remodeling by regulating the AMPK/PPARα signaling pathway in a rabbit model of atrial fibrillation.","authors":"Yang Wang, Zhe Sun, Zong-Tao Yin, Jian Zhang, Fang-Ran Xin, Yin-Li Xu, Huai Lan","doi":"10.32725/jab.2025.007","DOIUrl":"https://doi.org/10.32725/jab.2025.007","url":null,"abstract":"<p><p>Atrial fibrillation (AF) is a common arrhythmia encountered in clinical practice, characterized by myocardial fibrosis and atrial remodeling as its primary pathological features, and associated with significantly high mortality and disability rates. Currently, there are no specific pharmacological treatments for AF, and traditional anti-arrhythmic drugs have not achieved the desired efficacy, often resulting in a high incidence of adverse drug reactions. Thus, there is an urgent need for the development of novel anti-AF medications. Berberine, the main active component of Coptis chinensis, has been shown to have antiarrhythmic and anti-heart failure effects. However, its potential to improve atrial fibrosis and remodeling resulting from AF remains largely unexplored. In this study, we used a rapid atrial pacing (RAP) procedure to establish a rabbit model of AF associated with atrial fibrosis. Our objective was to assess the inhibitory effects of berberine on myocardial fibrosis, evaluate its impact on atrial remodeling, and investigate its underlying molecular mechanisms. Our findings indicate that berberine reduces left atrial weight and the area of myocardial fibrosis, inhibits the expression of α-SMA protein in atrial tissue, and decreases the levels of inflammation and oxidative stress. In addition, berberine effectively inhibits atrial remodeling, which may contribute to the prevention of AF. Through transcriptomics, molecular docking, and molecular dynamics simulations, we have tentatively confirmed that berberine may activate the AMPK-PPARα signaling pathway by directly binding to AMPK and PPARα, thereby improving atrial fibrillation.</p>","PeriodicalId":14912,"journal":{"name":"Journal of applied biomedicine","volume":"23 2","pages":"63-79"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}