Seleno-chitooligosaccharide-induced modulation of intestinal barrier function: Role of inflammatory cytokines, tight junction proteins, and gut microbiota in mice.

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2025-03-01 Epub Date: 2025-03-27 DOI:10.32725/jab.2025.003
Zhuo-Hong He, Ye Jin, Die Chen, Hui-Xin Zheng, Jia-Er Xiang, Yong-Jun Jiang, Zheng-Shun Wen
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引用次数: 0

Abstract

This study aimed to explore the function of Seleno-chitooligosaccharide (SOA) on the intestinal barrier through regulation of inflammatory cytokines, tight junction protein, and gut microbiota in mice. The results of ELISA assay demonstrated that SOA significantly increased the levels of IL-2, IL-10, and IFN-γ in serum and ileum. Meanwhile, SOA increased the levels of IL-4 in the ileum (p < 0.05). In addition, Diamine Oxidase (DAO) concentration was decreased in ileum by SOA treatments (p < 0.05). The administration of SOA significantly upregulated the expression of ZO-1 and Occludin in the ileum (p < 0.05). By 16S rDNA sequencing, reduced ratio of Bacillota/Bacteroidota was observed in SOA treated mice. Within the phylum of Bacteroidota, SOA increased the relative abundance of Deferribacterota, uncultured Bacteroidales bacterium, and Bacteroides. Within the phylum of Bacillota, increased relative abundance of Erysipelatoclostridium and Lachnoclostridium, and reduced relative abundance of Ruminococcaceae UCG-010 were observed with SOA supplement. In summary, SOA has the potential to modulate the function of intestinal barrier function and prevent intestinal diseases.

硒-壳寡糖诱导的肠道屏障功能调节:炎症因子、紧密连接蛋白和肠道微生物群在小鼠中的作用。
本研究旨在探讨硒壳寡糖(selenium -chitooligosaccharide, SOA)通过调节小鼠炎症因子、紧密连接蛋白和肠道菌群对肠道屏障的作用。ELISA检测结果显示,SOA显著提高血清和回肠中IL-2、IL-10和IFN-γ的水平。同时,SOA可提高回肠IL-4水平(p < 0.05)。此外,SOA处理降低了回肠中二胺氧化酶(DAO)浓度(p < 0.05)。SOA显著上调回肠ZO-1和Occludin的表达(p < 0.05)。通过16S rDNA测序,观察到SOA处理小鼠中杆状杆菌/拟杆菌的比例降低。在拟杆菌门内,SOA增加了铁杆菌门、未培养拟杆菌门和拟杆菌门的相对丰度。在杆状菌门中,添加SOA后丹毒杆菌和Lachnoclostridium的相对丰度增加,Ruminococcaceae UCG-010的相对丰度降低。综上所述,SOA具有调节肠道屏障功能和预防肠道疾病的潜力。
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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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