Chemical composition and anticancer activity of Psychotria montana on MCF7 breast cancer cells: insights from in vitro (2D & 3D) studies and in silico analysis.

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2025-03-01 Epub Date: 2025-03-21 DOI:10.32725/jab.2025.002
Hoang Van Hung, Thi Kieu Oanh Nguyen, Phu Hung Nguyen, Thi Thanh Huong Le, Viet Hoang
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引用次数: 0

Abstract

Aim: This study aimed to investigate the phytochemical composition of Psychotria montana extract (PME) and evaluate its inhibitory effects on MCF7 breast cancer cells.

Methods: The chemical composition of PME was analyzed using UPLC-QToF-MS. The effects of PME on cell proliferation were evaluated using the MTT assay. Flow cytometry was used for cell cycle and apoptosis analysis. The effects of PME on the transcription of cell cycle control genes were assessed using real-time PCR.

Results: UPLC-QToF-MS analysis revealed major compounds of PME, including terpenoids and flavonoids, with the potential to inhibit proliferation, migration, and induce apoptosis in MCF7 cancer cells. PME effectively suppressed MCF7 cell proliferation under 2D culture, with a low IC50 value of 34.7 µg/ml. PME also hindered cell migration (p < 0.01) and reduced spheroid number (p < 0.001) and size (p < 0.001) in serum-free 3D culture. Apoptosis analysis via nuclear staining with DAPI and flow cytometry revealed an increase in the number of apoptotic cells after PME treatment (p < 0.001). Additionally, the PME induced cell cycle arrest at the G0/G1 phase (p < 0.05). PME altered the expression of cell cycle control genes (cyclins and CDKs) as well as cancer suppressor genes including p16, p27, and p53 at the transcriptional level (mRNA). The results of molecular docking suggest that the compounds present in PME exhibit a high binding affinity for CDK3, CDK4, CDK6, and CDK8 proteins, which are essential regulators of the cell cycle.

Conclusion: Psychotria montana has the potential to inhibit cancer cells by inducing apoptosis and halting the cell cycle of MCF7 breast cancer cells.

蒙大拿精神草对MCF7乳腺癌细胞的化学成分和抗癌活性:来自体外(2D和3D)研究和硅分析的见解。
目的:研究蒙大拿精神提取物(PME)的植物化学成分,并评价其对MCF7乳腺癌细胞的抑制作用。方法:采用UPLC-QToF-MS对PME进行化学成分分析。采用MTT法评价PME对细胞增殖的影响。流式细胞术检测细胞周期和凋亡。实时荧光定量PCR检测PME对细胞周期调控基因转录的影响。结果:UPLC-QToF-MS分析显示,PME的主要化合物包括萜类化合物和黄酮类化合物,具有抑制MCF7癌细胞增殖、迁移和诱导凋亡的潜力。PME能有效抑制MCF7细胞在二维培养下的增殖,IC50值较低,为34.7µg/ml。在无血清3D培养中,PME还能阻碍细胞迁移(p < 0.01),减少球体数(p < 0.001)和大小(p < 0.001)。DAPI核染色和流式细胞术细胞凋亡分析显示PME处理后凋亡细胞数量增加(p < 0.001)。此外,PME诱导细胞周期阻滞在G0/G1期(p < 0.05)。PME在转录水平(mRNA)改变了细胞周期控制基因(cyclins和CDKs)以及肿瘤抑制基因p16、p27和p53的表达。分子对接结果表明,PME中存在的化合物对细胞周期的重要调节因子CDK3、CDK4、CDK6和CDK8蛋白具有高结合亲和力。结论:蒙大拿州精神草可能通过诱导MCF7乳腺癌细胞凋亡和停止细胞周期来抑制癌细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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