Journal Biomed最新文献_第3页

Comparative Immunogenicity of the Gam-COVID-Vac Vaccine under Intranasal and Intramuscular Administration Gam-COVID-Vac 疫苗鞘内和肌肉注射的免疫原性比较

Journal Biomed Pub Date : 2023-12-04 DOI: 10.33647/2074-5982-19-4-56-69

E. I. Katorkina, I. V. Lyagoskin, E. N. Bocharova, M. V. Bolotnikova, A. A. Borzov, N. V. Belskaya, A. E. Lisova, K. V. Ulyanova, N. K. Kudina, N. A. Belyanina, O. M. Strizhakova, A. S. Pershin, Ya. A. Bahareva, P. E. Kargopolova, A. P. Vasiliev, A. A. Kazarov, M. Lykov, R. A. Khamitov

{"title":"Comparative Immunogenicity of the Gam-COVID-Vac Vaccine under Intranasal and Intramuscular Administration","authors":"E. I. Katorkina, I. V. Lyagoskin, E. N. Bocharova, M. V. Bolotnikova, A. A. Borzov, N. V. Belskaya, A. E. Lisova, K. V. Ulyanova, N. K. Kudina, N. A. Belyanina, O. M. Strizhakova, A. S. Pershin, Ya. A. Bahareva, P. E. Kargopolova, A. P. Vasiliev, A. A. Kazarov, M. Lykov, R. A. Khamitov","doi":"10.33647/2074-5982-19-4-56-69","DOIUrl":"https://doi.org/10.33647/2074-5982-19-4-56-69","url":null,"abstract":"In this work, we set out to compare the immunogenicity of the Gam-COVID-Vac two-component vaccine produced by GENERIUM JSC (Russia) following its intranasal or intramuscular administration of the first or both components to BALB/c mice. The immunogenicity was evaluated according to antigen-specific IgG and IgA antibodies in the blood and bronchoalveolar fluid, the number of antigen-specific IFN-γ-producing T-lymphocytes, the number of antigen-specific IFN-γ-producing CD4 and CD8 T-lymphocytes. Intranasal administration was shown to induce the mucosal immunity, significantly exceeding both qualitatively and quantitatively the effect of intramuscular administration (by the number of animals with antigen-specific IgA antibodies and the titer of antibodies in blood serum and bronchoalveolar lavage). At the same time, intramuscular administration slightly exceeded the effect obtained under intranasal administration in terms of the total number of CD8-IFN-γ-producing lymphocytes.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"47 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138602354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of a New Dimethylamineethanol Derivative Administered Orally to Laboratory Animals 实验动物口服二甲胺乙醇新衍生物的药代动力学

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-110-113

A. Kim, E. B. Shustov

{"title":"Pharmacokinetics of a New Dimethylamineethanol Derivative Administered Orally to Laboratory Animals","authors":"A. Kim, E. B. Shustov","doi":"10.33647/2713-0428-19-3e-110-113","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-110-113","url":null,"abstract":"A new derivative of dimethylaminoethanol, butanedioic and trans-butenedioic acids (laboratory code ADK-17) was synthesized at the Organic Chemistry Department of the Saint Petersburg State Chemical and Pharmaceutical University (SPSPU). This promising nootropic and anti-asthmatic agent is planned for use in an oral dosage form. The aim was to calculate the pharmacokinetic parameters of the synthesized compound and to determine its possible biotransformation pathways. Rabbits of the Soviet Chinchilla breed were used as a test system in the preclinical study of the compound pharmacokinetics. In accordance with the designed schedule, blood was taken from the marginal vein of the ear, and urine samples were taken. Quantitative determination in biological media was carried out using an Ultimate 3000 liquid chromatograph with a Q-Exactive mass-selective detector with electrospray ionization in accordance with OFS 1.2.1.100015 (Chromatography) of the State Pharmacopoeia by ultra-performance liquid chromatography–mass spectrometry (UPLC-MS). To study the pharmacokinetics of the ADK-17 preparation, a measurement procedure was developed with a detection limit in biological media ranging from 10-6 to 10-3 mg/ml. The determined harmacokinetic parameters show that the absorption of ADK-17 into the systemic circulation from the gastrointestinal tract occurs at an average rate, with its maximum concentration in blood plasma during the oral route of administration being observed between 60–90 min. The elimination half-life from the systemic circulation comprises 4.3 h. Urinary excretion in the first 4 h proceeds at a high rate; however, the compound is detected in the urine within 3 days and in the blood within 2 days from the moment of administration.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139252010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Etomersol Fumarate on Cognitive Functions of Rats During Mercury Acetate Poisoning 富马酸依托摩醇对醋酸汞中毒大鼠认知功能的影响

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-124-129

K. Shchepetkova, E. G. Batotsyrenova, E. B. Shustov, V. Kashuro, A. V. Sharabanov, N. P. Ramenskaya

引用次数: 0

Effect of L-thyroxine, a Human Serum Albumin Natural Ligand, on the Kinetics of β-amyloid Peptide Fibril Formation 人血清白蛋白天然配体 L-甲状腺素对 β 淀粉样肽纤维形成动力学的影响

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-114-118

E. Litus, E. Nemashkalova, A. A. Vologzhannikova, E. Deryusheva

引用次数: 0

Development and Preclinical Trials of Castomized Porous Titanium Implants with Bioactive Coatings in Model Systems 带有生物活性涂层的铸造多孔钛植入体在模型系统中的开发和临床前试验

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-104-109

P. A. Karalkin, K. G. Kudrin, D. Svyatoslavov, D. Usatov, I. V. Reshetov

{"title":"Development and Preclinical Trials of Castomized Porous Titanium Implants with Bioactive Coatings in Model Systems","authors":"P. A. Karalkin, K. G. Kudrin, D. Svyatoslavov, D. Usatov, I. V. Reshetov","doi":"10.33647/2713-0428-19-3e-104-109","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-104-109","url":null,"abstract":"This paper presents a technology for creating customized porous titanium implants with bioactive coatings, manufactured using additive technologies. The stages of creating an implant include obtaining primary data from the bone defect area using computed tomography; 3D modeling of the defect area and the corresponding implant; production of a customized implant from titanium alloys using selective laser fusion technology; application of bioactive coatings. The as-created customized implant can have several functional structures. Samples of titanium implants with bioactive coatings were subjected to extensive testing. Mathematical modeling and experiments were used to verify the correspondence of the mechanical properties of the developed structures to natural bone tissue. In vitro tests of the studied samples showed the absence of acute toxicity along with high levels of biocompatibility. In vivo tests of the studied samples on Soviet chinchilla rabbits and Anubis baboon monkeys approved by local ethical committees showed their adequate biomechanical and high osteoinductive properties. The successful results of preclinical studies, as well as toxicological and technical tests in certified laboratories, made it possible to create a registration dossier for state registration of customized porous titanium implants with bioactive coatings, manufactured using additive technologies.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Course and Therapy Methods of Aquagenic Urticaria 水源性荨麻疹的临床过程和治疗方法

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-130-135

O. M. Kudelina, E. Gantsgorn, Yu. S. Maklyakov, D. L. Tkachenko, L. A. Avakyan, P. A. Chubyreva, Y. G. Amirova, Yu. A. Karpova, A. N. Dulskaya, I. B. Meshi

引用次数: 0

The effect of L. reuteri on Nonspecific Resistance and Hematological Parameters of Laboratory Animals 纽崔莱对实验动物非特异性抵抗力和血液学参数的影响

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-119-123

A. Ovcharova, N. V. Belova

引用次数: 0

Influence of Dioscorea Extracts on Physical Load Tolerance in the Setting of Hypoxic and Temperature Factors 薯蓣皂苷提取物在缺氧和温度因素下对物理负荷耐受性的影响

Journal Biomed Pub Date : 2023-11-21 DOI: 10.33647/2713-0428-19-3e-99-103

A. Ivkina, D. Ivkin, E. B. Shustov, M. N. Povydysh

引用次数: 0

HLDF-6-H Peptide Exhibits an Anti-inflammatory Effect in the Development of Depression in an MPTP Model of Parkinson's Disease HLDF-6-H肽在帕金森病MPTP模型抑郁发展过程中表现出抗炎作用

Journal Biomed Pub Date : 2023-11-20 DOI: 10.33647/2713-0428-19-3e-90-94

S. A. Zozulya, O. Sokolov, N. Kost, N. V. Baimeeva, A. K. Dadayan, Yu. A. Zolotarev

{"title":"HLDF-6-H Peptide Exhibits an Anti-inflammatory Effect in the Development of Depression in an MPTP Model of Parkinson's Disease","authors":"S. A. Zozulya, O. Sokolov, N. Kost, N. V. Baimeeva, A. K. Dadayan, Yu. A. Zolotarev","doi":"10.33647/2713-0428-19-3e-90-94","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-90-94","url":null,"abstract":"In this work, we investigate the anti-inflammatory effect of the HLDF-6-H peptide (Thr-Gly-Glu-HseHis-Arg) in a model of the presymptomatic stage of Parkinson’s disease (PD). This peptide is a bioactive fragment of the HLDF leukocyte differentiation factor. The presymptomatic stage of PD was modelled by introducing moderate doses of MPTP toxin to C57Bl/6 mice. HLDF-6-H was administered intranasally at a dose of 300 μg/kg daily for three weeks. The severity of depression was determined by the time of immobilization in a Porsolt test. The serum levels of 10 steroids were determined using MS analysis. The activity of inflammatory markers leukocyte elastase (LE) and α1-proteinase inhibitor (α1-PI) was determined using kinetic methods. The development of an inflammatory process under the influence of MPTP is evidenced by an increase in the activity of α1-PI; the anti-inflammatory effect of the peptide is manifested in a decreased activity of LE and α1-PI. At the same time, the immobilization time of mice in a Porsolt test positively correlated with α1-PI and LE, which may indicate the role of inflammation in the development of depression. A positive correlation between estradiol and LE was found in the general sample of mice. In the group of mice treated with the peptide, α1-PI correlated positively with corticosterone and negatively with estradiol and androstenedione. It can be assumed that the anti-inflammatory effect of the peptide is associated with the regulation of corticosteroids and sex hormones. Hence, the applied experimental model confirmed the development of a depressive-like syndrome and neuroendocrine disorders in the presymptomatic stage of PD. The long-term use of HLDF-6-H has antidepressant and anti-inflammatory effects.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"57 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of a Nanoform of 9-phenyl-2,3,4,5,6,7,8,9-octahydro-1H-selenoxanthene on the Reproductive Function of White Rats 9-苯基-2,3,4,5,6,7,8,9-八氢-1H-硒氧蒽纳米形式对白鼠生殖功能的影响

Journal Biomed Pub Date : 2023-11-20 DOI: 10.33647/2713-0428-19-3e-85-89

O. V. Buyuklinskayа, S. M. Napalkova, E. D. Kubasova, D. S. Makarova

引用次数: 0