Journal Biomed最新文献_第10页

Chronic Compensated Tonsillopharingitis: Clinical and Microbiological Features 慢性代偿性扁桃体咽喉炎:临床和微生物学特征

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-46-51

E. K. Meloyan, A. Safronenko, E. Gantsgorn, V. Polyakova, S. D. Stagniev, A. A. Ilyina, E. Y. Romanova

引用次数: 1

Comparative Study of Injectable Implants Based on Microparticles of Various Biodegradable Materials in a Murine Model of Subcutaneous Implantation 不同可生物降解材料微颗粒可注射植入物在小鼠皮下植入模型中的比较研究

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-63-76

I. Demyanenko, A. Shishkina, Yu. S. Hats, N. V. Kalmykova

引用次数: 0

Method for Obtaining and Studying a Gel Agar-Based Medium to Preserve the Electrical Activity of Rat Brain Slices after their Long–Term Cryopreservation 凝胶琼脂基大鼠脑切片长期冷冻保存电活动介质的制备与研究

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-31-39

A. Mokrushin

{"title":"Method for Obtaining and Studying a Gel Agar-Based Medium to Preserve the Electrical Activity of Rat Brain Slices after their Long–Term Cryopreservation","authors":"A. Mokrushin","doi":"10.33647/2074-5982-18-2-31-39","DOIUrl":"https://doi.org/10.33647/2074-5982-18-2-31-39","url":null,"abstract":"Cryopreservation biotechnology allows a long-term preservation makes it possible to preserve and subsequent recovery of biological objects for a long time. It This technology is used for creating is necessary to create a cryobanks. In this work, wWe have developeded a two-component freezing solution consisting of an artificial cerebrospinal fluid and agar in different concentrations. The effectiveness of the solution in terms offor long-term cryopreservation was investigated on surviving slices of the olfactory cortex of the brain of such non-hibernating animals – as rats. Changes Variations in the activities of AMPA and NMDA glutamatergic mechanisms in brain slices were studied as functional indicators of successful cryopreservation. The following agar Different concentrations of agar were used: 33%, 44% and 50%. At a concentration of 33% agar, AMPA hyperactivation and recovery of NMDA recovery mechanisms were observed. At a concentration of 44% agar, hyperactivation of both mechanisms occurred. A cComplete recovery of the activities of the AMPA and NMDA mechanisms after prolonged cryopreservation (–10°C, 52 days) was achieved at an agar concentration of 50%. The developed freezing agar-based freezing solution developed and studied by us does not contains no “heavy” protectors (DMSO), antibiotics, and cations, such as Ba2+ and Sr2+, which normally lead to an irreversible blockade of AMPA and NMDA mechanisms. Thus, an the agar-based freezingdeveloped solution solution helpscontributes to maintaining a high level of activity of AMPA and NMDA activity mechanisms in slices during their cryopreservation. The developed solution can be used to create a cryobank of nervous tissue.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80873553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Spontaneous Reports as a Methodological Pharmacovigilance Tool 自发报告作为方法学药物警戒工具的分析

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-40-45

T. S. Litvinenko, A. Safronenko, Yu. S. Maklyakov, E. Gantsgorn, E. S. Postnikova, M. O. Gaisaev, V. G. Brizhak

引用次数: 1

Impact of Intranasal Insulin Administration On Na+/K+-Atpase and Са2+-Transporting System Components in Rat Cardiomyocytes with Type 1 Diabetes Mellitus 鼻内胰岛素对1型糖尿病大鼠心肌细胞Na+/K+- atp酶和Са2+-转运系统组分的影响

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-52-62

I. Sukhov, O. Chistyakova

引用次数: 0

Stability of the sdha, hprt, prl3d1 and hes1 Gene Expression in a Rat Liver Fibrosis Model 大鼠肝纤维化模型中sdha、hprt、prl3d1和hes1基因表达的稳定性

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-17-30

E. I. Lebedeva, A. S. Babenko, A. Shchastniy

{"title":"Stability of the sdha, hprt, prl3d1 and hes1 Gene Expression in a Rat Liver Fibrosis Model","authors":"E. I. Lebedeva, A. S. Babenko, A. Shchastniy","doi":"10.33647/2074-5982-18-2-17-30","DOIUrl":"https://doi.org/10.33647/2074-5982-18-2-17-30","url":null,"abstract":"So far, no versatile set of reference genes for normalizing real-time polymerase chain reaction data has been identified. Numerous studies focusing the selection of reference genes for specific purposes frequently fail to elaborate a suitable selection strategy. In a number of such studies, the stage of selecting reference genes is ignored due to either its high cost or other reasons. As a result, the normalization of data is carried out using genes, which have previously shown their effectiveness under other, sometimes completely different, experimental conditions. In this work, we aim to study variations in the level of mRNA expression of several genes, some of which are commonly used to normalize RT-PCR data. As special conditions, modeling of rat liver fibrosis with thioacetamide was used.In our experiment, when considering the process of fibrogenesis as a whole, the optimal reference genes were found to be hes1 and sdha. However, when focusing on specific stages of fibrosis, a pair of genes should be selected depending on the stability indicators. At the initial fibrogenesis stages, sdha and hprt can be used. The hes1 gene is suitable as a reference gene, when the average Cq value of the target genes is approximately 29 cycles (as in hes1). Hes1 should be used with care when working in the Cq ranges of target genes of 26–29 and above 30, since the error is likely to increase. Following the same principle, the optimum Cq value for the sdha gene was observed to be 27, although the Cq range of 24–27 is also acceptable. At the same time, when working in the Cq range of above 28, the use of sdha may be associated with an increase in calculation errors.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79408604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of the Digestive Tract on Enzyme Activity 消化道对酶活性的影响

Journal Biomed Pub Date : 2022-06-10 DOI: 10.33647/2074-5982-18-2-10-16

D. Ksenofontov, A. A. Ksenofontova

{"title":"Influence of the Digestive Tract on Enzyme Activity","authors":"D. Ksenofontov, A. A. Ksenofontova","doi":"10.33647/2074-5982-18-2-10-16","DOIUrl":"https://doi.org/10.33647/2074-5982-18-2-10-16","url":null,"abstract":"Enzyme activity at the digestive tract level was experimentally studied on rabbits and mini-pigs. The enzyme activity in the blood flow supplied to and from the rabbit stomach and intestines was assessed. In rabbits, the arteriovenous oxygen difference revealed a decrease in the lactate dehydrogenase and amylase activity under an increase in the alkaline phosphatase activity. A regular dependence in the dynamics of gamma-glutamyl transferase activity in the intestinal mucosa and chyme fractions was established. It was found that gamma-glutamyl transferase activity decreases in the distal direction in the mucosa of the small intestine wall. Its maximum activity was determined in the soluble fraction of the small intestine chyme, with a characteristic decrease in the distal direction. Due to the hydration of the cavitary mucus glycoproteins in the chyme and the existence of the latter in the form of an enteroplasm, the enzyme is assumed to localize in the enteric medium between the soluble and dense endogenous fraction with the purpose of displaying the maximum transport activity. A conclusion was made about the plasma-forming role of the digestive tract in terms of blood enzyme activity.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80784524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technological Aspects of Obtaining Liposomes Containing of a Complex of Biologically Active Substances Isolated from Deer Musk 麝香生物活性物质复合物脂质体的制备工艺研究

Journal Biomed Pub Date : 2021-12-06 DOI: 10.33647/2074-5982-17-4-18-37

S. L. Lyublinskiy, I. N. Lyublinskaya, E. Koloskova, A. Azizov, V. Karkischenko, M. Nesterov, A. Kaptsov, R. Ageldinov, V. N. Gerasimov, D. Grinenko

{"title":"Technological Aspects of Obtaining Liposomes Containing of a Complex of Biologically Active Substances Isolated from Deer Musk","authors":"S. L. Lyublinskiy, I. N. Lyublinskaya, E. Koloskova, A. Azizov, V. Karkischenko, M. Nesterov, A. Kaptsov, R. Ageldinov, V. N. Gerasimov, D. Grinenko","doi":"10.33647/2074-5982-17-4-18-37","DOIUrl":"https://doi.org/10.33647/2074-5982-17-4-18-37","url":null,"abstract":"In order to preserve and increase the biological effectiveness of biologically active substances isolated from deer musk, we studied technological aspects of obtaining a substance of lipid-stabilized stable nanoparticles from deer musk. The stability of the obtained substance was evaluated. It was found that homogenization under high pressure is an optimal approach to obtaining biologically active substances from deer musk. The modes of preparation of a liposomal form of biologically active substances with predetermined dispersion parameters (average particle diameter 250 ± 100 nm, polydispersity index 0.3 ± 0.1, and zeta potential from -5 to -35 mV) were determined. It was found that the high-pressure homogenizer “Donor-5” makes it possible to obtain liposomal dispersions with standard parameters and the degree of inclusion of musk biologically active substances up to 60%, at the same time as providing minimal oxidation and hydrolysis of phospholipids (oxidation index 0.3). Our studies showed that the use of a domestic phosphatidylcholine is economically justiﬁed and allows obtaining liposomal forms of proper quality. The quality indicators of the obtained liposomal substance were characterised by conventional analytical methods (dynamic light scattering, electron microscopy, gel chromatography, chromatography-mass spectrometry, etc.). On the basis of the results obtained, a draft speciﬁcation was developed for a liposomal substance (powder) containing a complex of biologically active substances isolated from deer musk. The developed technology for obtaining a liposomal form of biologically active substances from deer musk can be scaled up in accordance with GMP requirements.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"127 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77105908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Burn Wound Healing Activity of Prunella Grandiﬂora L. Exctract 桔梗提取物烧伤创面愈合活性研究

Journal Biomed Pub Date : 2021-12-06 DOI: 10.33647/2074-5982-17-4-50-56

E. Bolotnik, L. Alekseeva, L. P. Larionov

引用次数: 0

Methods of Experimental Modeling of Metastasis 转移瘤的实验建模方法

Journal Biomed Pub Date : 2021-12-06 DOI: 10.33647/2074-5982-17-4-44-49

N. Timofeeva, N. V. Bubnova, G. Struchko, O. Kostrova, I. S. Stomenskaya

引用次数: 1