Iranian Journal of Parasitology最新文献_第8页

Comparing the Yield of Recombinant Human Factor VII Protein Expressed by the rDNA-Promoter with the CMV-Promoter in Iranian Lizard Leishmania. 比较伊朗蜥蜴利什曼原虫中 rDNA-Promoter 和 CMV-Promoter 表达的重组人因子 VII 蛋白的产量。

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15855

Afshin Abdi Ghavidel, Mojgan Bandehpour, Effat Noori, Vahid Jajarmi, Bahram Kazemi

{"title":"Comparing the Yield of Recombinant Human Factor VII Protein Expressed by the rDNA-Promoter with the CMV-Promoter in Iranian Lizard Leishmania.","authors":"Afshin Abdi Ghavidel, Mojgan Bandehpour, Effat Noori, Vahid Jajarmi, Bahram Kazemi","doi":"10.18502/ijpa.v19i2.15855","DOIUrl":"10.18502/ijpa.v19i2.15855","url":null,"abstract":"Background: Iranian Lizard Leishmania (I.L.L) is a nonpathogenic Leishmania strain. Due to its advantages, several recombinant proteins have been produced in this host. However, I.L.L shows a lower yield of recombinant protein expression compared to other commercial hosts. Considering the role of protease enzymes in protein digestion, we selected cysteine protease B (CPB) to investigate its impact on recombinant protein yield in I.L.L.Methods: we generated gene knockouts by utilizing homologous recombination (HR) and CRISPR methods. To assess the efficacy of the designed construct, we compared the yield of recombinant human factor VII (rhFVII) production between cells transfected with the pLEXSY-hyg2-FVII vector and the CMV-promoter-based construct (pF7cmvneo).Results: The knockout of a single CPB gene allele through the HR method or the complete knockout of all alleles through the CRISPR method led to cell death. This outcome suggests that even the deletion of a single CPB gene allele diminishes the protein to a level insufficient for the survival of I.L.L, indicating a critical dependency on the presence of this protein for the organism's viability. rhFVII exhibited a greater expression yield with the pLEXSY construct compared to the pF7cmvneo construct in I.L.L. The lower expression rate of pF7cmvneo may be influenced by epigenetic factors related to the CPC gene or the RNA polymerase used for the expression of that promoter.Conclusion: Therefore, considering alternative integration targets for CMV-promoter-based constructs and incorporating UTR sequences of I.L.L high-expression proteins in the vector may enhance recombinant protein expression rates.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"192-202"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Hepatic Phase of Fascioliasis Complicated with Liver Bleeding: Presentation of Three Cases. 并发肝出血的法氏囊病肝阶段：三个病例的介绍

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15863

Feyzullah Uçmak, Muhsin Kaya

引用次数: 0

Diagnosis and Treatment of Fasciola Infection with Endoscopic Retrograde Cholangiopancreatography (ERCP) in Ardabil, Northwest of Iran: Two Case Reports. 在伊朗西北部的阿尔达比勒通过内镜逆行胰胆管造影术 (ERCP) 诊断和治疗法氏囊感染：两个病例报告。

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15861

Shabnam Asfaram, Behnam Sajedi, Behnam Mohammadi-Ghalehbin

引用次数: 0

Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation. 为单核细胞衍生的外泌体制备设计针对大利什曼原虫和婴儿利什曼原虫的多表位候选疫苗

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15851

Ali Moazezi Ghavihelm, Sedigheh Nabian, Shahram Jamshidi, Mohammad Taheri, Minoo Soltani, Ramin Mazaheri Nezhad Fard, Ali Akbari Pazoki

{"title":"Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation.","authors":"Ali Moazezi Ghavihelm, Sedigheh Nabian, Shahram Jamshidi, Mohammad Taheri, Minoo Soltani, Ramin Mazaheri Nezhad Fard, Ali Akbari Pazoki","doi":"10.18502/ijpa.v19i2.15851","DOIUrl":"10.18502/ijpa.v19i2.15851","url":null,"abstract":"Background: Leishmania is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (GP63) and rK39 proteins against Leishmania major and L. infantum for monocyte-derived exosome preparation.Methods: This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of GP63 and rK39 using various immunoinformatics and bioinformatics approaches. Vibrio cholerae β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.Results: This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.Conclusion: A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further in-vitro and in-vivo studies.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"153-161"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of a Combined Herbal Product Containing Indigofera argentea, Pistacia atlantica and Salvia hispanica in Zoonotic Cutaneous Leishmaniasis Patients Treated along Glucantime ®. 含茵陈、桔梗和丹参的复合草药产品对使用 Glucantime ® 治疗的人畜共患皮肤利什曼病患者的疗效。

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15856

Saeid-Ali Bozorgzade, Shahram Kalantar-Khandani, Rezvan Amiri, Tania Dehesh, Hossein Karegar-Borzi

{"title":"Efficacy of a Combined Herbal Product Containing Indigofera argentea, Pistacia atlantica and Salvia hispanica in Zoonotic Cutaneous Leishmaniasis Patients Treated along Glucantime ®.","authors":"Saeid-Ali Bozorgzade, Shahram Kalantar-Khandani, Rezvan Amiri, Tania Dehesh, Hossein Karegar-Borzi","doi":"10.18502/ijpa.v19i2.15856","DOIUrl":"10.18502/ijpa.v19i2.15856","url":null,"abstract":"Background: Leishmaniasis is highly prevalent worldwide, and while conventional medicine offers numerous treatment methods for cutaneous leishmaniasis, Iranian traditional medicine suggests various remedies. We aimed to evaluate the efficacy of an herbal combination containing Indigofera argentea leaves, Pistacia atlantica resin, and Salvia hispanica seeds in patients with zoonotic cutaneous leishmaniasis.Methods: This study was conducted at the Leishmaniasis Department of Chabahar Health Center in southeastern Iran in 2021. It was a double-blinded randomized clinical trial involving 68 patients enrolled after clinical diagnosis, examination of smear samples using Giemsa staining, and PCR confirmation. The volunteers were randomly divided into intervention and control groups. Both groups received 'Glucantime ® as the primary medication weekly until complete healing or up to 12 weeks. Glucantime ® was administered intralesionally at a dosage of 0.1 cc on the wound's margin, with repeat injections at 1 cm intervals along the wound edge when necessary. In addition to the main drug, the intervention group received the herbal product topically twice daily until wound healing or for up to 4 weeks, while the control group received a placebo in the same manner.Results: 82.35% of patients in the intervention group and 20.58% in the control group achieved complete healing within four weeks. There was a significant difference between the two groups (P < 0.001).Conclusion: The herbal product demonstrated effectiveness in treating patients with zoonotic cutaneous leishmaniasis.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"203-213"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Nanonanoliposomal Curcumin on Cutaneous Leishmaniasis Skin Lesions Caused by Leishmania major in BALB/c Mice. 纳米脂质体姜黄素对 BALB/c 小鼠由大利什曼病引起的皮肤利什曼病皮损的评估

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15859

Sedigheh Shirmohammad, Mahdi Mohebali, Fatemeh Arabkhazaeli, Jalal Hassan, David Shayan, Narges Amininia, Parviz Shayan

{"title":"Evaluation of Nanonanoliposomal Curcumin on Cutaneous Leishmaniasis Skin Lesions Caused by Leishmania major in BALB/c Mice.","authors":"Sedigheh Shirmohammad, Mahdi Mohebali, Fatemeh Arabkhazaeli, Jalal Hassan, David Shayan, Narges Amininia, Parviz Shayan","doi":"10.18502/ijpa.v19i2.15859","DOIUrl":"10.18502/ijpa.v19i2.15859","url":null,"abstract":"Background: Curcumin is an extract of rhizome turmeric (diferuloylmethane), with antioxidant, anti-inflammatory, antimicrobial, and anti-parasitic properties, which making it a potential candidate for the treatment of leishmaniasis. The aim of the presented study was to evaluate curcumin as possible candidate for treatment of cutaneous leishmaniasis.Methods: We investigated the physicochemical properties and anti-leishmanial effects of nanoliposomal curcumin (40, 80, and 120 μM) in Leishmania major (MRHO/IR/75/ER) infected BALB/c mice at the faculty of Veterinary Medicinem University of Tehran, Iran. For this aim, L. major promastigotes (MHROM/IR/75/ER) at stationary phase (2×106) were inoculated sub-cutaneously into the upper area of the tail in BALB/c mice (six groups, n= 10 per group). For evaluation of nanoliposomal curcumin, the zeta potential, particle size and stability of nanoliposomal curcumin was determined. Furthermore, the anti-leishmanial effects of nanoliposomal curcumin formulation on the lesion sizes was determined and the parasite burden in the leishmania induced lesion was performed using semi quantitative PCR.Results: Treatment of L. major infected BALB/c mice with nanoliposomal curcumin led to a reduction in the kinetic of the skin lesion size development. The semi quantitative PCR analysis of DNA extracted from the lesions showed reduction of parasite burden. The most effective treatment could be found in 80 μM nanoliposomal curcumin. Treatment with Glucantime, as a positive control, also showed a nearly similar effect compared to the effect of 80 μM nanoliposomal curcumin.Conclusion: Nanoliposomal curcumin could be considered as a potential drug against cutaneous leishmaniasis caused by L. major in susceptible animal models.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"238-246"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Nanos and Ago Genes Expression in the Germinative Cells Isolated from Germinal Layer and the Neck Region of Echinococcus granulosus. 从颗粒棘球蚴胚芽层和颈部分离的胚芽细胞中 Nanos 和 Ago 基因表达的比较分析

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15849

Amin Yazdani, Narges Khamesi, Alireza Keyhani, Saeid Nasibi, Mohammad Ali Mohammadi, Seyed Mohammad Mousavi, Ali Derakhshani, Majid Fasihi Harandi

{"title":"Comparative Analysis of Nanos and Ago Genes Expression in the Germinative Cells Isolated from Germinal Layer and the Neck Region of Echinococcus granulosus.","authors":"Amin Yazdani, Narges Khamesi, Alireza Keyhani, Saeid Nasibi, Mohammad Ali Mohammadi, Seyed Mohammad Mousavi, Ali Derakhshani, Majid Fasihi Harandi","doi":"10.18502/ijpa.v19i2.15849","DOIUrl":"10.18502/ijpa.v19i2.15849","url":null,"abstract":"Background: We aimed to evaluate the differential expression of nanos and ago genes in the protoscoleces, germinal layer, the neck, and the sucker regions of adult Echinococcus granulosus.Methods: The study was conducted in 2018 at the Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. In the present study E. granulosus protoscoleces were cultured in a di-phasic medium to obtain strobilated worms. The strobilated worms were harvested and using a sterile razor blade, the neck region was separated. In the molecular study the neck sections were compared with the tissues derived from the suckers from the same worm. The primers were specifically designed for RT-qPCR on nanos and ago. The germinative cells were isolated from the cyst germinal layer and cultured in DMEM for further molecular studies. The Immunohisto-chemical profile was designed to explore the nature of nanos protein in the strobilated worms. Differences between and within groups were statistically assessed relative to the protoscoleces.Results: An increasing nanos gene expressions were found in sucker, neck, cells and germinal layer in comparison to the protoscoleces. The expression of ago gene was decreased in sucker, cell and germinal layer, and increased in the neck region in comparison to the protoscoleces. The results showed that both genes were expressed in all developmental stages of E. granulosus.Conclusion: nanos and ago genes were differentially expressed at different developmental stages of E. granulosus and may contribute to differentiation of the parasite.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"131-139"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Molecular Identification of Vahlkampfiidae and Vermamoeba Vermiformis from Fresh Vegetables: A Neglected Source of Infections. 新鲜蔬菜中 Vahlkampfiidae 和 Vermamoeba Vermiformis 的分离和分子鉴定：被忽视的感染源。

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15854

Marziye Fatemi, Maryam Niyyati, Seyed Ahmad Karamati, Hamed Mirjalali

{"title":"Isolation and Molecular Identification of Vahlkampfiidae and Vermamoeba Vermiformis from Fresh Vegetables: A Neglected Source of Infections.","authors":"Marziye Fatemi, Maryam Niyyati, Seyed Ahmad Karamati, Hamed Mirjalali","doi":"10.18502/ijpa.v19i2.15854","DOIUrl":"10.18502/ijpa.v19i2.15854","url":null,"abstract":"Background: Naegleria spp., Tetramitus spp., and Vermamoeba vermiformis are potential pathogenic free-living amoebae (FLA) causing diseases such as keratitis, meningoencephalitis, and lung infections. We aimed to investigate the presence of Vahlkampfiidae and V. vermiformis in raw vegetables commonly consumed in Iran.Methods: Totally, 70 samples of vegetables samples including watercress (22), leeks (12), parsley (10), basil (13) and mint (13) were collected from municipal markets of Tehran, the capital of Iran during June to October 2021. After washing vegetables, samples were cultivated onto 2% non-nutrient agar (NNA) medium. After morphological confirmations, DNA was extracted and identical fragments of the FLA were amplified and sequenced.Results: Out of 70 cultured samples, 11 samples (15.71 %) were morphologically positive, of which four and seven were V. vermiformis and Vahlkampfiidae isolates, respectively. According to the PCR/sequencing results two, one, one, and one strains belonged to N. australiensis, N. americana, Vahlkampfia sp., V. inornata, and T. aberdonicus, respectively. All Vermamoeba genus were characterized as V. vermiformis.Conclusion: The results of current study revealed the contamination of fresh raw vegetables with Vahlkampfiidae and V. vermiformis. In addition, to our knowledge this is the first report of T. aberdonicus in raw vegetables. Our findings highlight the public health importance of vegetables in transmission of FLA, as well as the potential role of FLA in transmission of potential pathogenic microorganisms via consuming of fresh raw vegetables.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"183-191"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic and Immunomodulatory Activity of Curcumin and Chitosan on Experimental Toxoplasmosis. 姜黄素和壳聚糖对实验性弓形虫病的细胞毒性和免疫调节活性

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15858

Mahsa Rezgi, Elham Yousefi, Behzad Jafari, Negar Asadi, Shahram Khademvatan, Gordon S Howarth

{"title":"Cytotoxic and Immunomodulatory Activity of Curcumin and Chitosan on Experimental Toxoplasmosis.","authors":"Mahsa Rezgi, Elham Yousefi, Behzad Jafari, Negar Asadi, Shahram Khademvatan, Gordon S Howarth","doi":"10.18502/ijpa.v19i2.15858","DOIUrl":"10.18502/ijpa.v19i2.15858","url":null,"abstract":"Background: Toxoplasma gondii is a pathogenic parasite with worldwide distribution. We investigated curcumin and chitosan in combination on the viability of T. gondii tachyzoites in silico, in vitro and in vivo.Methods: A 3D model was employed in Urmia University of Medical Sciences, Urmia, Iran in 2021 to study the interaction between curcumin and dihydrofolate reductase (DHFR). Ramachandran root-mean-square deviation and VERIFY3D validated the model. Cytotoxicity of curcumin and chitosan was evaluated by MTT viability assay. BALB/c mice infected with 104 Toxoplasma organisms were treated with curcumin, chitosan, and the combination of curcumin+chitosan. Serum levels of inducible NO synthetase (iNOs), interferon gamma (IFN-γ), interleukin (IL)-5, glutamate oxaloacetic transaminases(SGOT), and glutamic pyruvate transaminase (SGPT) were determined.Result: Curcumin-DHFR and curcumin-DHPS (dihydropteroate synthase) interactions and calculated enzyme energy indicated an excellent affinity for curcumin with DHFR, but not DHPS. MTT results of concurrent treatments demonstrated IC50 rates of 0.1, 0.05, and 0.01 mg/ml at 24, 48, and 72h, respectively. IFN-γ, IL-5 and iNOs levels in curcumin+chitosan treated mice were 1.71, 0.51, and 1.51 IU/L, while those of SGOT and SGPT were 76 and 84 IU/L, respectively.Conclusion: The combination of curcumin and chitosan increased survival time of infected mice by seven days. Curcumin and chitosan in combination regulated the immune system and reduced liver damage, potentially forming the basis of a new treatment for toxoplasmosis.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"224-237"},"PeriodicalIF":0.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postpartum Spontaneous Rupture of a Primary Splenic Hydatid Cyst: A Case Report. 原发性脾包虫囊肿产后自发性破裂：病例报告。

IF 0.9 4区医学

Iranian Journal of Parasitology Pub Date : 2024-04-01 DOI: 10.18502/ijpa.v19i2.15862

Aabid Ashraf, Ritika Mittal

引用次数: 0