Ali Moazezi Ghavihelm, Sedigheh Nabian, Shahram Jamshidi, Mohammad Taheri, Minoo Soltani, Ramin Mazaheri Nezhad Fard, Ali Akbari Pazoki
{"title":"为单核细胞衍生的外泌体制备设计针对大利什曼原虫和婴儿利什曼原虫的多表位候选疫苗","authors":"Ali Moazezi Ghavihelm, Sedigheh Nabian, Shahram Jamshidi, Mohammad Taheri, Minoo Soltani, Ramin Mazaheri Nezhad Fard, Ali Akbari Pazoki","doi":"10.18502/ijpa.v19i2.15851","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Leishmania</i> is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (<i>GP63</i>) and <i>rK39</i> proteins against <i>Leishmania major</i> and <i>L. infantum</i> for monocyte-derived exosome preparation.</p><p><strong>Methods: </strong>This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of <i>GP63</i> and <i>rK39</i> using various immunoinformatics and bioinformatics approaches. <i>Vibrio cholerae</i> β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.</p><p><strong>Results: </strong>This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.</p><p><strong>Conclusion: </strong>A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further <i>in-vitro</i> and <i>in-vivo</i> studies.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 2","pages":"153-161"},"PeriodicalIF":0.9000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246201/pdf/","citationCount":"0","resultStr":"{\"title\":\"Designing a Multiple-Epitope Vaccine Candidate against <i>Leishmania major</i> and <i>Leishmania infantum</i> for Monocyte-Derived Exosome Preparation.\",\"authors\":\"Ali Moazezi Ghavihelm, Sedigheh Nabian, Shahram Jamshidi, Mohammad Taheri, Minoo Soltani, Ramin Mazaheri Nezhad Fard, Ali Akbari Pazoki\",\"doi\":\"10.18502/ijpa.v19i2.15851\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Leishmania</i> is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (<i>GP63</i>) and <i>rK39</i> proteins against <i>Leishmania major</i> and <i>L. infantum</i> for monocyte-derived exosome preparation.</p><p><strong>Methods: </strong>This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of <i>GP63</i> and <i>rK39</i> using various immunoinformatics and bioinformatics approaches. <i>Vibrio cholerae</i> β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.</p><p><strong>Results: </strong>This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.</p><p><strong>Conclusion: </strong>A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further <i>in-vitro</i> and <i>in-vivo</i> studies.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"19 2\",\"pages\":\"153-161\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246201/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v19i2.15851\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v19i2.15851","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation.
Background: Leishmania is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (GP63) and rK39 proteins against Leishmania major and L. infantum for monocyte-derived exosome preparation.
Methods: This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of GP63 and rK39 using various immunoinformatics and bioinformatics approaches. Vibrio cholerae β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated.
Results: This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis.
Conclusion: A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further in-vitro and in-vivo studies.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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