Investigative ophthalmology & visual science最新文献

{"title":"F4H5 Alleviates Increased Outflow Resistance Induced by Emulsified Silicone Oil In Vitro.","authors":"Marcel Muuss, Margarita Karaivanova, Lea Skrzypczyk, Sabrina Wohlfart, Jonathan Herth, Philipp Uhl, Gerd Uwe Auffarth, Maximilian Hammer","doi":"10.1167/iovs.66.11.21","DOIUrl":"10.1167/iovs.66.11.21","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to investigate the effect of emulsified silicone oil on trabecular outflow resistance using an in vitro perfusion model of human and porcine anterior segments. F4H5, an approved washout for emulsified silicone oil in the posterior segment, was evaluated as a potential rescue treatment.</p><p><strong>Methods: </strong>Seventeen porcine and 15 human anterior segments were dissected and clamped into an in vitro perfusion model. The intraocular pressure (IOP) was recorded continuously and evaluated before and after the injection of emulsified silicone oil, followed by treatment with F4H5 in the intervention group. Control eyes received injections of balanced salt solution (BSS).</p><p><strong>Results: </strong>In human eyes, the injection of emulsified silicone oil significantly increased IOP from 7.8 ± 1.3 millimeters of mercury (mm Hg) to 15.8 ± 3.5 mm Hg (increase of 8.0 ± 2.3 mm Hg, P = 0.02, mean time to stabilized higher pressure = 8.6 hours). Subsequent treatment with F4H5 reverted the IOP back to near baseline values (10.8 ± 2.2 mm Hg, P = 0.06 compared to baseline, mean time to stabilized lower pressure = 6.9 hours). Porcine eyes demonstrated comparable trends, with a significant IOP increase from 14.5 ± 1.2 mm Hg to 17.7 ± 1.6 mm Hg after silicone oil injection (increase of 3.2 ± 0.9 mm Hg, P = 0.01) and a subsequent significant drop in IOP to 12.4 ± 1.0 mm Hg after the injection of F4H5 (decrease of 5.3 ± 1.1 mm Hg, P < 0.01).</p><p><strong>Conclusions: </strong>Emulsified silicone oil leads to blockage of the outflow pathways and an increased IOP in an in vitro human and porcine anterior segment perfusion model. With F4H5, a clinically relevant reversal of this increased outflow resistance was achieved and should be further evaluated.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"21"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12347274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Loss of RORα Impairs Visual Function With Rod Bipolar Cell Degeneration In Mice.","authors":"Kiran Bora, Chi-Hsiu Liu, Neetu Kushwah, Meenakshi Maurya, Madeline C Pavlovich, Laura A Solt, Ye Sun, Zhongjie Fu, James D Akula, Jing Chen","doi":"10.1167/iovs.66.11.20","DOIUrl":"10.1167/iovs.66.11.20","url":null,"abstract":"<p><strong>Purpose: </strong>Retinoic acid receptor-related orphan receptor alpha (RORα) is a ligand-dependent nuclear receptor involved in eye development and diseases. Genetic variations of RORα have been linked with the development of age-related macular degeneration, yet whether RORα regulates visual function is unknown. In this study we investigated the role of RORα in regulating visual function in mice with genetic deficiency of RORα.</p><p><strong>Methods: </strong>RORα deficient staggerer (Rorasg/sg) and age-matched wild type control mice were assessed for visual function using electroretinography (ERG). Retinal localization of RORα and rod bipolar cell morphology were assessed using immunohistochemistry. Retinal thickness was measured as well as presence of retinal gliosis, and expression of genes in visual transduction.</p><p><strong>Results: </strong>RORα is expressed in photoreceptors, retinal ganglion cells, and bipolar cells. Genetic deletion of RORα in Rorasg/sg mice markedly impaired visual function measured by ERG, with substantial attenuation of b-wave amplitude compared with wild type controls, reflective of bipolar cell dysfunction. Rorasg/sg eyes showed significant degeneration of rod bipolar cells and retinal gliosis, both of which progressed upon aging. Moreover, loss of ROR⍺ also resulted in significant retinal thinning, including both outer and inner nuclear layers. Expression of genes involved in phototransduction and rod bipolar cell depolarization was upregulated in Rorasg/sg retinas, suggestive of potential compensatory remodeling of visual signaling.</p><p><strong>Conclusions: </strong>Our findings demonstrate that RORα is indispensable for the structural and functional maintenance of rod bipolar cells and could pose as a potential therapeutic target for mitigating bipolar cell deficits in retinal degenerative diseases.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"20"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12347186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polarization-Sensitive Optical Coherence Tomography Imaging of Posterior Staphyloma Edges in Eyes With High Myopia.","authors":"Yijin Wu, Hongshuang Lu, Changyu Chen, Jianping Xiong, Masahiro Yamanari, Hiroyuki Takahashi, Keigo Sugisawa, Michiaki Okamoto, Yining Wang, Ziye Wang, Tomonari Takahashi, Koju Kamoi, Kyoko Ohno-Matsui","doi":"10.1167/iovs.66.11.6","DOIUrl":"10.1167/iovs.66.11.6","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the arrangement and orientation of the collagen fibers in the inner and outer scleral layers at the edges of posterior staphylomas using polarization-sensitive optical coherence tomography (PS-OCT).</p><p><strong>Methods: </strong>This observational case series study enrolled 105 patients with high myopia (refractive error <-6.0 diopters or axial length ≥26.5 mm) who underwent PS-OCT examinations between August 2023 and May 2024 at the Institute of Science Tokyo. Posterior staphyloma was diagnosed in 88 eyes of 60 patients using ultra-widefield OCT, followed by PS-OCT image processing. Thirty-six eyes from 27 patients with clearly identifiable staphyloma edges were included for final analysis. PS-OCT images were analyzed to assess the scleral fiber orientation and birefringence at the staphyloma edges, including macular and inferior types.</p><p><strong>Results: </strong>Optic axis images showed that 25 (18 upper and 7 lower) of 30 (83.3%) macular staphyloma edges had an aggregation of the inner scleral fibers, characterized by an arc-like gathering of the horizontal fibers along the edge. All eight upper edges of the eight eyes with an inferior staphyloma had an aggregation of the inner scleral fibers with relatively linear horizontal fibers across the macula. Birefringence images of the upper and lower edges of the macular and inferior staphylomas had different patterns, including a mixture of low and high, and relatively high birefringence.</p><p><strong>Conclusions: </strong>An uneven aggregation of inner scleral fibers at the staphyloma edges in highly myopic eyes offers novel insights into staphyloma pathogenesis. This finding also holds pivotal implications for developing targeted interventions to prevent the progression of staphylomas in high myopia.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"6"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12338364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celastrol Activates SIRT1/PGC-1α/Nrf2 Axis to Inhibit Oxidative Damage for Subconjunctival Fibrosis Alleviation.","authors":"Zhihua Guo, Huayang Feng, Xue Sun, Liu Yang, Qingyun Ning, Yuting Deng, Zhanrong Li, Jingguo Li","doi":"10.1167/iovs.66.11.70","DOIUrl":"https://doi.org/10.1167/iovs.66.11.70","url":null,"abstract":"<p><strong>Purpose: </strong>Subconjunctival fibrosis can lead to symblepharon and recurrent pterygium, seriously threatening vision. Our previous research found that celastrol could inhibit subconjunctival fibrosis. The purpose of this study was to investigate the underlying mechanisms of celastrol-nanomicelles (Cel) in inhibiting subconjunctival fibrosis.</p><p><strong>Methods: </strong>We established rat subconjunctival injury model in vivo and TGF-β1-activated human pterygium fibroblasts systems in vitro to study fibrotic pathogenesis. RNA-seq analysis was used to identify the targets of Cel. Immunofluorescence and Western blot analyses were performed to determine whether Cel modulates the SIRT1/PGC-1α/Nrf2 axis, thereby alleviating oxidative damage and subconjunctival fibrosis in vitro. The critical involvement of the SIRT1/PGC-1α/Nrf2 axis was definitively established through pharmacological inhibition using EX527, a selective SIRT1 antagonist. Moreover, the effects of Cel on subconjunctival fibrosis in vivo were further explored through histopathological assessments, Masson's trichrome staining, and immunofluorescence.</p><p><strong>Results: </strong>In vivo evaluation with a rat subconjunctival injury model showed that Cel effectively attenuated oxidative damage and subconjunctival fibrosis. The RNA-seq analysis indicated that SIRT1 signal and oxidative stress regulation as candidate targets of Cel for subconjunctival fibrosis. The results of immunofluorescence and Western blot showed that Cel inhibited subconjunctival fibrosis by activating SIRT1/PGC-1α/Nrf2 axis and suppressing TGF-β1/Smad2/3 pathway, while the SIRT1 inhibitor eliminated the effects of Cel.</p><p><strong>Conclusions: </strong>This work demonstrated that Cel effectively alleviated subconjunctival fibrosis by inhibiting oxidative damage via activating SIRT1/PGC-1α/Nrf2 signaling pathway, providing a new potential mechanism to clarify Cel as a potential therapeutic drug to inhibit subconjunctival fibrosis.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"70"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction of: Feet on the Ground: Physical Support of the Inner Retina Is a Strong Determinant for Cell Survival and Structural Preservation In Vitro.","authors":"","doi":"10.1167/iovs.66.11.54","DOIUrl":"https://doi.org/10.1167/iovs.66.11.54","url":null,"abstract":"","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"54"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Response: Letter to the Editor Regarding \"Intraretinal Hyper-Reflective Foci Are Almost Universally Present and Co-Localize With Intraretinal Fluid in Diabetic Macular Edema\".","authors":"Esther L von Schulthess, Andreas Maunz, Usha Chakravarthy, Nancy Holekamp, Daniel Pauleikhoff, Katie Patel, Isabel Bachmeier, Siqing Yu, Yaniv Cohen, Mahnaz Parian-Scherb, Ian L Jones, Kara Gibson, Jeffrey R Willis, Carl Glittenberg, Rishi P Singh, Sascha Fauser","doi":"10.1167/iovs.66.11.36","DOIUrl":"10.1167/iovs.66.11.36","url":null,"abstract":"","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"36"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting the Structure-Function Relationship in Glaucoma Using a Physiological Model.","authors":"Chris Bradley, Jithin Yohannan","doi":"10.1167/iovs.66.11.75","DOIUrl":"10.1167/iovs.66.11.75","url":null,"abstract":"<p><strong>Purpose: </strong>Determine how well a physiological model-the retina-V1 (RV1) model of target detection-predicts the structure-function relationship in glaucoma.</p><p><strong>Methods: </strong>Unlike curve-fitting models, the RV1 model includes a map of retinal ganglion cell (RGC) receptive fields across the visual field (VF), enabling simulation of different patterns of RGC loss. Predicted mean sensitivity for different patterns of simulated RGC loss and predictions of different curve-fitting models were compared to 12,917 paired SITA-Standard 24-2 VFs and optical coherence tomography measurements of average retinal nerve fiber layer thickness from 4432 eyes of 2418 patients with glaucoma between 1997 and 2023. Except for one free parameter, all RV1 model parameters were fixed from a previous study that fit the model to an unrelated data set of contrast sensitivities for 43 localized achromatic stimuli.</p><p><strong>Results: </strong>Different structure-function relationships were predicted by the RV1 model for different patterns of RGC loss. Random RGC loss resulted in a mean absolute error of 2.99 dB, which was marginally but significantly smaller than 3.01 dB for ninth-degree polynomial regression-the highest degree polynomial whose coefficients could be estimated. Other patterns of simulated RGC loss, including periphery-to-fovea loss typical in glaucoma, accounted for much of the observed variance in the structure-function data. Unlike curve-fitting models, the RV1 model correctly predicted higher variance at lower dB/micron levels.</p><p><strong>Conclusions: </strong>A physiological model can account for much of the observed variance in structure-function data for glaucoma by simulating different patterns of RGC loss-this is currently not possible with curve-fitting models.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"75"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Retinal Antigen-Presenting Cells in Spontaneous Retinal Autoimmunity.","authors":"Joe Sherman, Laura Burgstaler, Yunan Li, Heidi Roehrich, Dale S Gregerson, Scott W McPherson","doi":"10.1167/iovs.66.11.26","DOIUrl":"10.1167/iovs.66.11.26","url":null,"abstract":"<p><strong>Purpose: </strong>We reported earlier that induction of spontaneous autoimmune uveoretinitis (SAU) correlated with the recruitment of circulating antigen-presenting cells (APCs) into the retina. Here, we investigated the role of resident retinal APCs on the course of SAU.</p><p><strong>Methods: </strong>R161H+/- mice (B10.RIII), which spontaneously and rapidly develop severe autoimmune uveoretinitis, were crossed with CD11cDTR/GFP mice (B6/J). R161H+/- mice on the B6/J background develop slower, less severe SAU than R161H+/--B10.RIII mice, allowing assessment of disease development relative to the depletion or activation of retinal or systemic APCs. The course of SAU was established in a cohort of control R161H+/- × CD11cDTR/GFP F1 mice and then reanalyzed in test cohorts following treatment with diphtheria toxin or stimulation by optic nerve crush (ONC) injury. Analysis was done by retinal imaging, flow cytometry, and histology.</p><p><strong>Results: </strong>Systemic depletion of APCs halted the progression of SAU in R161H+/- × CD11cDTR/GFP F1 mice and, if commenced early in the disease process, would reduce SAU severity. However, following depletion of APCs specifically from the retina, SAU in R161H+/- × CD11cDTR/GFP F1 mice progressed in a manner similar to that of control mice. In contrast, SAU in R161H+/- × CD11cDTR/GFP F1 mice was exacerbated following the activation of retinal APCs by ONC.</p><p><strong>Conclusions: </strong>Our observations that local depletion of retinal APCs failed to inhibit SAU progression and that depletion of circulating APCs not only limited SAU progression but also, under defined circumstances, reduced clinical SAU support the idea that circulating APCs are crucial for the induction and progression of SAU.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"26"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12352516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Omental Mesothelial Cells Exhibit a Corneal Endothelial-Like Cell Phenotype for Tissue Engineering of a Corneal Endothelium Biomimetic.","authors":"Jorge L Alió, Benoit R Gauthier, Juan C Gómez Rosado, Jorge L Alió Del Barrio, Alejandra E Rodríguez, Francisco J Díaz Corrales, Luis C Capitán Morales, Bernat Soria, Christian C Lachaud","doi":"10.1167/iovs.66.11.7","DOIUrl":"10.1167/iovs.66.11.7","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate whether cultured human omental mesothelial cells (OMC) exhibit phenotypic and functional similarities to human corneal endothelial cells (CEC) and whether they can adhere to the corneal stroma and form a biomimetic corneal endothelium when grown on a human anterior lens capsule (HALC).</p><p><strong>Methods: </strong>Human OMC were isolated from the greater omentum. Human B4G12 CEC were used as a reference for native and functional CEC phenotype whereas human mesenchymal stromal cells (MSC) served as a phenotypically distinct control group. OMC, CEC and MSC were compared using flow cytometry, immunofluorescence and qRT-PCR for lineage-specific markers, as well as through a transepithelial permeability assay. The adhesion capacity of OMC was also evaluated on both mouse corneal stroma and a decellularized HALC.</p><p><strong>Results: </strong>Several cell-surface markers commonly expressed at high levels in MSC (CD13, CD105, CD73 and CD44) were detected at markedly lower levels in OMC and CEC. OMC and CEC, but not MSC, also shared a similar expression profile of key structural markers (pan cytokeratin, ZO-1, β-catenin, N-cadherin, COL4A2, COL8A2) and functional corneal endothelium markers (AQP1, ATP1A1, SLC4A11, CLCN-3). In addition, OMC demonstrated transepithelial permeability values comparable to those of CEC monolayers supporting their barrier-forming capacity. Both CEC and OMC formed cobblestone-like monolayers on mouse corneal stroma and OMC seeded onto HALC generated a bioconstruct that reproduced the key morphological and ultra-structural features of native corneal endothelium.</p><p><strong>Conclusions: </strong>Overall, our findings show that cultured human OMC exhibit phenotypic and functional similarities to CEC. The ability of OMC to adhere to the corneal stroma and to generate a biomimetic corneal endothelium when combined with HALC highlights their potential use in corneal endothelial regeneration.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"7"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12347251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foveal Identification and Development in Prematurity-Implications on Zone Localization and Nutritional Supplementation.","authors":"Sarthak V Shah, Arthur R Brant, Akwasi Ahmed, Naana A Wireko Brobby, Cindy S Zhao, Ashna Ahya, Geoffrey C Tabin, Darius M Moshfeghi","doi":"10.1167/iovs.66.11.17","DOIUrl":"10.1167/iovs.66.11.17","url":null,"abstract":"<p><strong>Purpose: </strong>Accurate identification of retinal Zone I in retinopathy of prematurity (ROP) is critical for treatment decisions and prognosis. Current definitions rely on identifying the macular center, limited by absence of the foveal light reflex (FLR) early in screening. Understanding factors influencing FLR development could improve zone localization and guide nutritional interventions. We sought to determine whether absolute infant weight gain independently predicts FLR development in premature infants, beyond postmenstrual age (PMA).</p><p><strong>Methods: </strong>Prospective observational cohort study conducted from November 2024 to March 2025 within a multicenter telemedicine-based ROP screening program (GHANAROP). Premature infants meeting ROP screening criteria, screened before 37 weeks PMA, who subsequently developed FLR identifiable on wide-field digital retinal imaging were included. Logistic regression assessed associations between FLR presence, PMA, and infant weight. Multivariate logistic regression with elastic net regularization evaluated combined predictive value of PMA and weight.</p><p><strong>Results: </strong>Among 318 eyes, FLR was first identified at mean PMA of 35 0/7 weeks (low risk ROP) and 35 2/7 weeks (more than low risk ROP), and mean weight of 1723g (low risk) and 1565g (more than low risk). Univariate modeling found that each additional week of PMA increased odds of FLR presence by 44.6% (OR = 1.446; 95% CI, 1.371-1.524) and that each 200g increase in weight increased odds by 23.1% (OR = 1.231; 95% CI, 1.113-1.360). Multivariate modeling demonstrated PMA and weight as predictors of FLR development, achieving 84.8% predictive accuracy (Nagelkerke R² = 0.2783).</p><p><strong>Conclusions: </strong>Absolute infant weight independently predicts FLR development beyond PMA alone, highlighting opportunities for nutritional interventions to accelerate foveal maturation and potentially enhance visual outcomes.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 11","pages":"17"},"PeriodicalIF":4.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12347157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}