Investigative ophthalmology & visual science最新文献

{"title":"Oridonin Preserves Retinal Pigmented Epithelial Cell Tight Junctions and Ameliorates Choroidal Neovascularization.","authors":"Juming Zhu, Dongmei Ding, Tao Sun, Yuting Zhang, Huizi Miao, Yunjie Gu, Ming Dai, Manhui Zhu","doi":"10.1167/iovs.66.2.56","DOIUrl":"10.1167/iovs.66.2.56","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the role and mechanism of oridonin (ORI), a bioactive diterpenoid extracted from the Chinese herbal medicine Rabdosia rubescens, on the integrity of outer blood-retinal barrier (oBRB) during choroidal neovascularization (CNV).</p><p><strong>Methods: </strong>ARPE-19 cells were exposed to hypoxia and treated with ORI. The expression of ZO-1 and occludin in the axis of TGFβR/SUV39H1/KLF11 was detected by WB, chromatin immunoprecipitation, luciferin report activity assay, and immunofluorescence assay (IF), and the effect of ORI on the barrier properties of ARPE-19 cells was studied. A laser-induced mouse CNV model was constructed, and ORI was administrated by oral gavage. IF on mouse choroid flat mounts was done to confirm the effect of ORI on BRB integrity. Indocyanine green angiography and IF on mouse retina-RPE-choroid flat mounts were performed to determine the effect of ORI on CNV formation and retinal function. Hematoxylin and eosin staining and TUNEL staining were carried out to appraise ocular and systemic cytotoxicity caused by ORI.</p><p><strong>Results: </strong>ORI protected ARPE-19 cells from hypoxia-induced destruction of barrier properties and promoted the expression of ZO-1 and occludin by the TGFβR/SUV39H1/KLF11 axis, maintaining barrier properties of ARPE-19 cells with hypoxia. ORI improved BRB integrity during laser-induced CNV in mice and mitigated laser-induced CNV formation in mice without any ocular or systemic cytotoxicity (n = 4-5 in each group).</p><p><strong>Conclusions: </strong>ORI ameliorates BRB integrity and subsequent formation of CNV via regulating the TGFβR/SUV39H1/KLF11 pathway in RPE cells.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"56"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, Enrichment, and Computational Modeling of Cross-Linked Actin Networks in Transformed Trabecular Meshwork Cells.","authors":"Haiyan Li, Devon H Harvey, Jiannong Dai, Steven P Swingle, Anthony M Compton, Chenna Kesavulu Sugali, Kamesh Dhamodaran, Jing Yao, Tsai-Yu Lin, Todd Sulchek, Taeyoon Kim, C Ross Ethier, Weiming Mao","doi":"10.1167/iovs.66.2.65","DOIUrl":"10.1167/iovs.66.2.65","url":null,"abstract":"<p><strong>Purpose: </strong>Cross-linked actin networks (CLANs) are prevalent in the glaucomatous trabecular meshwork (TM). We previously developed the GTM3L cell line, which spontaneously forms fluorescently labeled CLANs, by transducing GTM3, a transformed glaucomatous TM cell line, with a lentivirus expressing the LifeAct-GFP fusion protein. Here, we determined if LifeAct-GFP viral copy numbers are associated with CLANs, developed approaches to increase CLAN incidence, and computationally studied the biomechanical properties of CLAN-containing GTM3L cells.</p><p><strong>Methods: </strong>GTM3L cells were fluorescently sorted for viral copy number analysis to determine whether increased CLAN incidence was associated with copy number. CLAN incidence was increased by combining (1) differential adhesion sorting, (2) cell deswelling, and (3) cell stiffness selection. GTM3L cells were cultured on glass or soft hydrogels for stiffness measurement by atomic force microscopy. Computational models studied the biomechanical properties of CLANs.</p><p><strong>Results: </strong>GTM3L cells had one LifeAct-GFP viral copy/cell on average, and viral copy number or LifeAct-GFP expression level did not associate with CLAN incidence rate. However, CLAN rate was increased from -0.28% to -50% by combining the three enrichment methods noted above. Further, GTM3L cells formed more CLANs on a stiff versus a soft substrate. Computational modeling predicted that CLANs contribute to higher cell stiffness, including increased resistance of the nucleus to tensile stress when CLANs are physically linked to the nucleus.</p><p><strong>Conclusions: </strong>It is possible to greatly enhance CLAN incidence in GTM3L cells. CLANs are mechanosensitive structures that affect cell biomechanical properties. Further research is needed to determine the biomechanics, mechanobiology, and etiology of CLANs in the TM.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"65"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11878246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthropometric Measures and Fuchs' Endothelial Corneal Dystrophy: The Women's Health Initiative Observational Study.","authors":"Yihua Yue, Sangita P Patel, Jing Nie, Chris A Andrews, Jean Wactawski-Wende, Aladdin H Shadyab, Robert Wallace, Amy E Millen","doi":"10.1167/iovs.66.2.26","DOIUrl":"10.1167/iovs.66.2.26","url":null,"abstract":"<p><strong>Purpose: </strong>Both genetic and environmental factors contribute to the development of Fuchs' endothelial corneal dystrophy (FECD), the most common indication for corneal transplantation in the United States. Prior studies have suggested an association of height, weight, or body mass index (BMI) with FECD. We examined the association between anthropometric measures and incident FECD in the Women's Health Initiative Observational Study (WHI-OS) of postmenopausal women (n = 22,983).</p><p><strong>Methods: </strong>Medicare Part B claims data from the WHI-OS baseline visit (1993-1998) to 2019 were used to identify incident cases of FECD. At baseline and follow-up year 3, weight, height, waist circumference (WC), and hip circumference were measured. At baseline, women were asked to recall their historic weight at ages 18, 35, and 50 years. At follow-up years 1 and 4 to 8, the women were asked to self-report their weight. Height and weight were used to calculate BMI at each time point. Adjusted hazard ratios (HRs), 95% confidence intervals (CI), and p for trend for incident FECD were estimated by measures of historic BMI, baseline anthropometrics measures, and anthropometric measures that incorporated more than one baseline visit (time-varying). Anthropometric measures were parameterized as continuous and categorical in analyses.</p><p><strong>Results: </strong>There were 1399 incident FECD cases with an annualized incidence rate of 5.06 per 1000 person-years (95% CI = 4.80-5.33) over 276,443 person-years of follow-up. No statistically significant associations were observed between baseline height and risk of FECD. Women with baseline BMIs ≥35 (obesity II) compared to <25 kg/m2 (normal weight or underweight) had lower risk of incident FECD (HR = 0.68, 95% CI = 0.53-0.88) with a P value = 0.0373 for an ordinal trend analysis across BMI categories. Significant inverse associations were observed for continuous measures of WC (HR = 0.97, 95% CI = 0.95-0.99 per 5 cm increase) and waist-to-hip ratio (WHR; HR = 0.92, 95% CI = 0.86-0.99 per 0.1 unit increase). No statistically significant associations were observed for time-varying BMI, but time-varying WC and WHR has statistically significant inverse associations with risk for FECD (data not shown).</p><p><strong>Conclusions: </strong>In this cohort of postmenopausal women, BMI, WC, and WHR were inversely associated with incident FECD. These findings generally support the potential role of anthropometric measures, particularly those indicative of abdominal obesity in FECD susceptibility in women.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"26"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Optical Coherence Tomography Angiography Precede Clinical Onset of Placental Insufficiency.","authors":"Ye He, Pearl Heumann, Melissa Weilin Song, Shin Kadomoto, Srinivas R Sadda, Ilina D Pluym, Irena Tsui","doi":"10.1167/iovs.66.2.36","DOIUrl":"10.1167/iovs.66.2.36","url":null,"abstract":"<p><strong>Purpose: </strong>This prospective cohort study examined the use of optical coherence tomography angiography (OCTA) to detect in vivo retinal and choroidal variations associated with placental insufficiency, manifesting as fetal growth restriction (FGR) and hypertensive disorders of pregnancy (HDP).</p><p><strong>Methods: </strong>Pregnant women were imaged with OCTA from gestational week 16 and every 4 weeks until week 36. Pregnancy outcomes were categorized into three groups: uncomplicated, complicated by late FGR, or complicated by HDP after 32 weeks. OCTA metrics, including retinal perfusion density (PD), vessel length density (VLD), and choriocapillaris flow deficits (CCFDs), were compared between groups.</p><p><strong>Results: </strong>In uncomplicated pregnancies, OCTA metrics remained stable throughout gestation. In contrast, the FGR group exhibited significant overall increases in superficial VLD (P = 0.016), decreases in deep VLD (P = 0.029), and increases in CCFDs (P = 0.002) throughout pregnancy when compared to the uncomplicated group. The HDP group showed significant overall decreases in both PD (P < 0.001) and VLD (P = 0.019) in the deep layer when compared to the uncomplicated group. Furthermore, CCFDs demonstrated strong potential for early prediction of FGR as early as week 16 (area under the curve = 0.73; P = 0.012).</p><p><strong>Conclusions: </strong>Our pilot study highlights the potential of OCTA to identify retinal and choroidal variations as biomarkers for pregnancy complications, particularly increased CCFDs in FGR.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"36"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remote Preconditioning Provides Protection Against Retinal Cell Death From Retinal Detachment.","authors":"Bruna Miglioranza Scavuzzi, Sumathi Shanmugam, Mengling Yang, Jingyu Yao, Heather Hager, Bhavneet Kaur, Lin Jia, Steven F Abcouwer, David N Zacks","doi":"10.1167/iovs.66.2.34","DOIUrl":"10.1167/iovs.66.2.34","url":null,"abstract":"<p><strong>Purpose: </strong>Remote preconditioning involves injury to a tissue that results in protection to a subsequent injury in a distal tissue. Here, we investigated the impact of remote preconditioning on retinal detachment (RD) injury, hypothesizing that a previous contralateral RD would protect the fellow retina against inflammation and cell death following its detachment.</p><p><strong>Methods: </strong>RD was created in adult C57BL/6J mice with subretinal sodium hyaluronate injection. Preconditioning involved RD in the right eye at 1, 3, 7, or 28 days before left eye detachment, whereas the control group only received RD to the left eye. Retinas were harvested 24 hours post-left eye detachment in both groups. Cell death was assessed using Cell Death Detection ELISA and mRNA expression was evaluated via qRT-PCR.</p><p><strong>Results: </strong>Contralateral RD promoted a transient protection against retinal cell death from 1 to 3 days and waned by 7 days compared with control RD retinas with intact fellow retinas. Contralateral RD significantly protected against post-RD cell death (P = 0.0002) and caspase 3 cleavage (P = 0.0449), compared with control RD retinas with intact fellow retinas 1-day post-RD. Detached fellow retinas from the preconditioning group expressed significantly less Tnfa (P = 0.0066), Cxcl10 (P = 0.0099), and Fas (P = 0.0223) mRNAs, compared with the detached retinas of the control group. In contrast, upregulation of type-I-IFN pathway genes, including Irf7 (P = 0.0106) and Ifit1 (P = 0.0740), following RD was higher in the preconditioning group.</p><p><strong>Conclusions: </strong>RD in one eye produces a transient remote preconditioning effect that protects the fellow retina against retinal cell death following subsequent RD.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"34"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Levels of C5a Are Associated With Reduced Macular Sensitivity in Patients With Myeloproliferative Neoplasms.","authors":"Kathrine Gotfredsen, Andreas Abou-Taha, Charlotte Liisborg, Marie Krogh Nielsen, Morten Kranker Larsen, Vibe Skov, Lasse Kjær, Hans Karl Hasselbalch, Torben Lykke Sørensen","doi":"10.1167/iovs.66.2.41","DOIUrl":"10.1167/iovs.66.2.41","url":null,"abstract":"<p><strong>Purpose: </strong>Previous findings indicate that patients with myeloproliferative neoplasms (MPN) exhibit elevated levels of inflammatory biomarkers and have a high prevalence of AMD. In this study, we aim to determine whether drusen and systemic inflammation in patients with MPN affect macular sensitivity in the same manner as in patients with AMD.</p><p><strong>Methods: </strong>The study was conducted as a prospective cross-sectional study. A total of 139 study eyes of 71 patients were included in this study. We measured macular sensitivity using microperimetry and extracted blood samples to evaluate systemic inflammation markers.</p><p><strong>Results: </strong>Multilevel linear mixed-effect analysis did not show any difference in macular sensitivity when comparing eyes of MPN patients with AMD to those without drusen (β = -0.254, P = 0.657). However, higher levels of the complement system fragment C5a were significantly correlated with decreased total macular sensitivity (β = -0.561, P = 0.027), irrespective of the presence of drusen.</p><p><strong>Conclusions: </strong>We found that high levels of the systemic inflammation marker C5a are associated with reduced macular sensitivity, regardless of the presence of visible degenerative changes in the macular area. These findings suggest an early contribution of the complement system to macular sensitivity.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"41"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor Regarding \"Intraretinal Hyper-Reflective Foci Are Almost Universally Present and Co-Localize With Intraretinal Fluid in Diabetic Macular Edema\".","authors":"Edoardo Midena, Luisa Frizziero","doi":"10.1167/iovs.66.2.59","DOIUrl":"10.1167/iovs.66.2.59","url":null,"abstract":"","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"59"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations Between Visual Field Defects and Macular Thinning in Sickle Cell Disease.","authors":"Alexandre Bourdin, Brigitte Ranque, Edouard Flamarion, Jacques Charlier, Jean-Benoît Arlet, Christophe Orssaud","doi":"10.1167/iovs.66.2.67","DOIUrl":"10.1167/iovs.66.2.67","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to investigate the correlation between defects in automatic perimetry and macular temporal thinning in optical coherent tomography (OCT) on patients with sickle cell disease (SCD) to define the impact of its maculopathy on visual function.</p><p><strong>Methods: </strong>This single site retrospective cross-sectional study was conducted in a referral center for rare disease from January to July 2020. Fifty-eight patients with SCD were referred for an ophthalmological examination including automatic perimetry and OCT. The correlation between macular thickness and visual field defect in each Early Treatment Diabetic Retinopathy Study (ETDRS) quadrants was calculated.</p><p><strong>Results: </strong>Visual acuity was normal in 104 eyes (94.5%). Thirty-three eyes (30.0%) had a defect of visual field in at least one ETDRS quadrant. There was a significant correlation between macular thinning on OCT and decrease of the mean defect in visual field in the temporal quadrant (P < 0.001). When comparing sickle cell genotype groups, macular thickness was significantly reduced in temporal outer quadrants in patients with the SS genotype, and in the inner and outer temporal quadrants in other genotypes.</p><p><strong>Conclusions: </strong>Despite normal visual acuity, macular temporal thinning in patients with SCD is associated with visual field defects in SS genotypes. This points to a primitive vascular dysfunction rather than neurogenic mechanism in occurrence of SCD maculopathy.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"67"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling Neuropathic Corneal Pain: Pulled Nerve Approach With Elevated Krt16 Gene Expression.","authors":"Mohd Afzal Khan, Gehan Fatima, Acquah Emmanuel, Sang Seong Kim, Hyuk Sang Kwon, Kyung Chul Yoon, Young Ro Kim, Euiheon Chung","doi":"10.1167/iovs.66.2.35","DOIUrl":"10.1167/iovs.66.2.35","url":null,"abstract":"<p><strong>Purpose: </strong>Neuropathic corneal pain (NCP) is a debilitating condition affecting millions of people worldwide. Despite their critical importance, currently available animal models for NCP research are limited by complex surgeries with high-risk strategy. To advance fundamental understanding of NCP, we developed a novel rodent model that explores both structural and functional mechanisms of the disease, offering a comprehensive approach.</p><p><strong>Methods: </strong>By uplifting (2-3 mm transversely) the long ciliary nerve (LCN) with gentle force (0.09 ± 0.02 newton [N]) and pressure (0.18 ± 0.05 MPa), our pulled nerve model mimics human NCP conditions and was investigated alongside normal control, sham control, and full transection groups. Specifically, we quantified the NCP status by establishing a relationship between pain perception and chemical sensitivity, using Stevens' Power Law concept.</p><p><strong>Results: </strong>Following surgery, the temporal patterns of heightened pain perception showed consistent trends across different stimulus methods, suggesting that von Frey and chemical tests could effectively evaluate pain progression. The discernable differences in Alpha values (exponent) of the pain-perception curves across the normal control, pulled nerve, and full transection groups (0.175 ± 0.035, 0.235 ± 0.015, and 0.275 ± 0.005, respectively) demonstrate the model's sensitivity to changes in NCP status. Histological analysis revealed LCN elongation, thickening, and corneal alterations in pulled nerve models, with reduced satellite glial cells (SGCs) in trigeminal ganglion compared to the normal control models. Krt16 gene expression was significantly upregulated following pulled nerve surgery.</p><p><strong>Conclusions: </strong>Our model not only delineates the pathological landscape of NCP but also promises to accelerate the development of targeted therapies.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"35"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OCT-Based Retina Assessment Reflects Visual Impairment in Multiple Sclerosis.","authors":"Natacha Stolowy, Lilija Gutmann, Margareta Lüpke, Thierry David, Michael Dorr, Christina Mayer, Christoph Heesen, Frederike Cosima Oertel, Ting-Yi Lin, Friedemann Paul, Hanna Gwendolyn Zimmermann, Jan-Patrick Stellmann","doi":"10.1167/iovs.66.2.39","DOIUrl":"10.1167/iovs.66.2.39","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the relationship between visual performance and retinal morphology as assessed by optical coherence tomography (OCT), and the ability of OCT to reflect visual impairment in people with multiple sclerosis (PwMS) compared with healthy controls (HC).</p><p><strong>Methods: </strong>We gathered data from two neurology referral centers on PwMS and HC. Neurological and ophthalmological assessments, including OCT, high-contrast visual acuity (HCVA) and low-contrast visual acuity (LCVA), area under the log contrast sensitivity function (AULCSF), and vision-related quality of life (National Eye Institute Visual Function Questionnaire), were conducted between 2018 and 2020, with follow-up at 1 year.</p><p><strong>Results: </strong>A total of 137 PwMS (271 eyes) and 118 HC (236 eyes) were available for analysis. The peripapillary retinal nerve fiber layer (pRNFL) and the macular ganglion cell layer and inner plexiform layer volume (mGCIPL) volume were both reduced in PwMS (92 µm in PwMS vs 98 µm in HC [P < 0.001], 0.55 mm3 vs 0.62 mm3 [P < 0.001], respectively). A cutoff effect for visual impairment was observed in PwMS when pRNFL fell below 68.8 µm (HCVA), 71.4 µm (LCVA), and 72.6 µm (AULCSF). Using mixed effects models, the mGCIPL volume emerged as the variable most strongly associated with the AULCSF (P < 0.001). The AULCSF showed the strongest correlation with both pRNFL and mGCIPL (P < 0.001), with optic neuritis being a significant contributing factor (P < 0.001).</p><p><strong>Conclusions: </strong>AULCSF outperformed standard HCVA and LCVA, closely reflecting retinal atrophy. mGCIPL loss showed stronger associations with vision tests and detected neurodegeneration without the cutoff effect seen in pRNFL, making it the best marker for neuronal atrophy.</p>","PeriodicalId":14620,"journal":{"name":"Investigative ophthalmology & visual science","volume":"66 2","pages":"39"},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143407913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}