R. Golshani, A. Moradi, R. M. Nadushan, M. Fatemi, P. G. Mostafavi
{"title":"Organophosphorus pesticides (diazinon, malathion and azinfos methyl) accumulation in three fish species, in south coasts of the Caspian Sea, Iran","authors":"R. Golshani, A. Moradi, R. M. Nadushan, M. Fatemi, P. G. Mostafavi","doi":"10.22092/IJFS.2020.122933","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122933","url":null,"abstract":"In this study the distribution and accumulation of diazinon, malathion and azinfos methyl in mullet Liza aurata,Caspian white fish Rutilus kutum and common carp Cyprinus carpio from five estuaries along the Caspian Sea have been investigated. Also, the effects of pesticides concentrations on the acetylcholinesterase enzyme (AChE) in fish species were studies. Pesticides concentration varied with fish species, sampling station and toxins types. The results indicate that the pesticides concentrations varied from 0.01 to 0.16 mg kg-1 for diazinon, 0.01 to 0.15 mg kg-1 for malathion and 0.05 to 0.36 mg kg-1 for azinfos methyl in three fish species. There was significant difference between different toxins concentrations in fish species, (p diazinon>malathion. There was significant difference in toxins concentrations between three fish species, and the highest toxins concentrations were absorbed in detritivores fish (L. aurata), followed by herbivorous fish (C. carpio) and carnivore fish (R. kutum). The results confirmed that toxins bioaccumulation in fish species is strongly controlled by habitat and feeding habits.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3050-3062"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47461643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maryam Jamali, R. M. Nadushan, A. Javid, A. Moradi, M. Givianrad
{"title":"Spatial trends of Total Petroleum Hydrocarbons, related heavy metals and sediment characteristics in South Caspian Sea: Effect of depth and temporal dispersions","authors":"Maryam Jamali, R. M. Nadushan, A. Javid, A. Moradi, M. Givianrad","doi":"10.22092/IJFS.2020.122964","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122964","url":null,"abstract":"In this study, Amirabad Special Economic was used as the study area, the distribution and tracing possible sources TPH, nickel, lead, vanadium, TP and TN were analysed in coastal sediments of 5 transects. According to our results the inner part of the 2 ports, are going to be slightly contaminated with TPH and heavy metals; meaning the contents of all the sites were lower than the background values. Based on physico-chemical parameters of water and according to dbRDA, the separation of offshore and river area was observed. Based on TOM, TPH and sediment nutrients, PCoA separated river and Sadra port transects. Finally, it seems high inter-annual water MVDISP values, reflecting highly temporal dynamic condition, influence the transportation of sediments along a depth gradient proved by nMDS, and the consecutive resuspension of sediments to open water is strong enough to keep the contaminants from rising to very high levels.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3221-3238"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45755833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanieh Sadralsadati, A. Moradi, M. Afsharnasab, P. G. Mostafavi, E. Ramezani-Fard
{"title":"The effects of copper-based nanoparticles on the immune system of the white shrimp (Penaeus vannamei)","authors":"Hanieh Sadralsadati, A. Moradi, M. Afsharnasab, P. G. Mostafavi, E. Ramezani-Fard","doi":"10.22092/IJFS.2020.122945","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122945","url":null,"abstract":"The application and development of nanotechnology is increasingly expanded in many areas. However, considering this expansion, several concerns regarding their potential toxicity in marine biology have been addressed. Indeed, the overuse of these materials can adversely influence marine ecosystems and living organisms. Herein, the potential impact of copper-based nanoparticles on the expression level of three genes, i.e. prophenoloxidase, serine protein and glutathione peroxidase genes functioning in shrimp immune response, were assessed. For this end, the shrimps were exposed to three semi-acute toxicity treatments, including 0.25, 0.5 and 1 mg/L of CuNPs. The qRT-PCR results indicated negative effect of supplied nanoparticle on the expression level of these genes. Additionally, histopathological alterations in the hepatopancreas and lymphoid organs were observed in the shrimps after exposure in different concentrations of CuNPs. Overall, we showed that toxic concentration of CuNPs can damage shrimp immune system as well as some internal organs. These results open up novel insights into innate immunity of shrimps subjected to copper-based nanoparticles.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3170-3182"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44977306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gobalakrishnan, R. RadhikaRajasreeS, Karthih Mg, Aranganathan. L
{"title":"Spawning and larval rearing of red sea urchin Salmacis bicolor (L. Agassiz and Desor, 1846;Echinodermata: Echinoidea)","authors":"M. Gobalakrishnan, R. RadhikaRajasreeS, Karthih Mg, Aranganathan. L","doi":"10.22092/IJFS.2020.122939","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122939","url":null,"abstract":"Gonads of sea urchin attract consumers due to its high nutritional value than any other seafood delicacies. Aquaculturists are also very keen on developing larval culture methods for large-scale cultivation. The present investigation systematically examined the larval rearing, development, survival and growth rate of Salmacis bicolor fed with various microalgal diets under laboratory condition. Fertilization rate was estimated as 95%. The blastula and gastrula stages attained at 8.25 h and 23.10 h post-fertilization. The 4 - armed pluteus larvae were formed with two well - developed post-oral arms at 44.20 h following post-fertilization. The 8 - armed pluteus attained at 9 days post fertilization. The competent larva with complete rudiment growth was developed on 25th days post - fertilization. Monodiet algal feed - Chaetoceros calcitrans and Dunaliella salina resulted medium (50.6 ± 2.7%) and low survival rate (36.8 ± 1.7%) of S. bicolor larvae. However, combination algal feed – Isochrysis galbana and Chaetoceros calcitrans has promoted high survival rate (68.3 ± 2.5%) which was significantly different between the mono and combination diet. From the observations of the study, combination diet could be adopted as an effective feed measure to promote the production of nutritionally valuable roes of S. bicolor.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3098-3111"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47911412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Morshedi, N. Agh, F. Noori, Fatemeh Jafary, A. Qasemi
{"title":"Growth, body composition, physiological responses and expression of immune-related and growth-related genes of Sobaity seabream (Sparidentex hasta) juvenile fed dietary bovine lactoferrin","authors":"V. Morshedi, N. Agh, F. Noori, Fatemeh Jafary, A. Qasemi","doi":"10.22092/IJFS.2020.122972","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122972","url":null,"abstract":"Lactoferrin (LF) has been used as an immunostimulant in different fish species and have effects on growth and immunological parameters. The aim of this study was to evaluate the effects of dietary LF on growth and feeding performance, body composition, digestive enzymes activity, hematological factors, humoral immune response and expression of immune-related and growth-related genes in sobaity juvenile (Sparidentex hasta). One hundred and thirty five fish with an initial average weight of 35.64±0.30 g were fed for a period of 8 weeks. Basal diet was supplemented with 0 (control), 400 and 800 mg kg-1 LF in a completely randomized design trial in triplicate groups. The results showed no significant differences in growth and feeding parameters and body composition between control and treatment groups (p >0.05). Moreover, no significant difference was observed in digestive enzymes activity, non-specific immune response, hematological parameters and immune-related gene expression (IL-1β) between control and the treatment groups (p >0.05). Nonetheless, growth-related gene expression (IGF-I) of treatments and control group significantly varied (p <0.05). These results suggested that dietary LF used in this study is not appropriate for supplementation in the diet of sobaity juvenile as it did not influence the growth performance, feed utilization, body composition and the physiological parameters in this fish. This study encourages further research on different aspects of LF in sobaity culture with a clear emphasis on optimizing dosage levels.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3269-3284"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45457326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in brain and pituitary GnRH levels during a reproductive cycle in wild female Asian sea bass, Lates calcarifer (Bloch, 1790)","authors":"P. Xuan","doi":"10.22092/IJFS.2020.122971","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122971","url":null,"abstract":"Changes in the peptide levels of sea bream (Sparus aurata) GnRH (sbGnRH) in the olfactory bulb, telencephalon including the preoptic area, hypothalamus, optic tectum-thalamus and pituitary during a reproductive cycle were investigated in addition to plasma levels of testosterone (T), 17β- estradiol (E2) and 17α, 20b-dihydroxy-4-pregnen-3-one (DHP) in wild female Asian sea bass Lates calcarifer in Nha Phu lagoon, Vietnam from March to September, 2010. Ovaries were classified into five stages: II Recovering spent (March -April), III Maturing (May-June), IV Mature (June), V Ripe and ovulating (July- August) and VI Spent (September). The gonadosomatic index increased significantly from the stage II (1.62%±0.12) to stage V (8.48%±0.23) and dropped thereafter in stage VI (2.05%±0.25). In the olfactory bulb and telencephalon, sbGnRH levels in stage IV and stage V were slightly higher than those in the other stages, but no significant differences in sbGnRH levels were found among the ovarian stages. In the hypothalamus and optic tectum-thalamus, although significant differences in sbGnRH level were not found among the ovarian stages, the highest level of sbGnRH was determined in stage III (594.13±152.12 pg tissue-1 and 501.37±124.35 pg tissue-1, respectively). In the pituitary, sbGnRH level in stage III (6927.03±218.92 pg tissue-1) was significantly higher than that in the other stages. In addition, plasma T, E2 and DHP levels increased significantly during ovarian maturation or ovulation with the highest level of T (2.91±0.31 ng ml-1) and E2 (4.98±1.43 ng ml-1) recorded in stage IV and DHP (389.6 ± 136.03 pg ml-1) in stage V. The results showed that sbGnRH in the anterior brain and pituitary is involved in initiation and ovarian maturation in wild Asian sea bass.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3255-3268"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41760270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular diagnosis of pathogenic Aeromonas hydrophila in Cyprinus carpio ulcers","authors":"I. Ibrahim, Shamal Hamadamen, K. Karim","doi":"10.22092/IJFS.2020.122944","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122944","url":null,"abstract":"A total of 150 carp fish were collected from Grdarasha Farm (8 kilometers from Erbil-Iraq) and examined for the presence of pathogenic Aeromonas hydrophila on their skin ulcers, this bacterium secretes a toxin protein called aerolysin which is a virulence factor and has a role in diarrhoeal diseases, deep wound infections and hemorrhagic septicemia. The present study aimed to determine the presence of A. hydrophila in common carp (Cyprinus carpio). For this purpose samples were collected from fish ulcer by swap and incubated at 37°C in blood agar to determine the shape and morphology of colonies, and then the colonies were examined by gram stain test for microscopic examination, finally molecular diagnosis was conducted for aerolysin gene with primer design and PCR. According to the diagnosis methods results were different; PCR diagnosis confirmed that only 68 skin lesions were caused by A. hydrophila, no visceral lesion was caused by A. hydrophila and this bacterium hardly ever seen in the skin surrounding mouth as only 2 lesions surrounding mouth had A. hydrophila in PCR diagnosis.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3160-3169"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunological, hematological, and histological responses in blood, spleen and gill of Salmo caspius juveniles exposed in different water temperatures","authors":"H. Khara","doi":"10.22092/IJFS.2020.122842","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122842","url":null,"abstract":"Caspian brown trout, Salmo caspius, were subject to five temperatures (5°C, 8°C, 20°C, 25°C, and 28°C) for two durations 12 and 24 hours. The fish were maintained for 48 hours at a water temperature of 9-10°C to acclimatize.The water temperature was changed gradually at a rate of 2°C per hour to the respective temperatures. Exposure to temperatures of 25 and 28°C resulted in loss of all fish. Red blood cell, hemoglobin concentration, and hematocrit were decreased at 5°C compared to control (8°C) and 20°C in duration of 12h. Temperature tension did not affect the mean cell volume and mean cell hemoglobin value during the 12 and 24h duration (p >0.05). Neutrophils percentage was significantly higher at 5°C and 20°C, but the most percentage of lymphocytes was noted in the control group. Cortisol, glucose, and lactate values elevated in both periods when water temperature decreased to 5°C compare to the control and 20°C groups (p <0.05). The levels of albumin and total protein in the duration of 12h at 20°C were significantly higher than control and 5°C, but this trend was not seen in the exposure of 24h (p <0.05). The lysozyme activity and immunoglobin M were affected by varying temperatures (p <0.05), and highest levels were observed in control groups. Most histopathological changes in gill, such as epithelial hypertrophy, curling of lamella, and necrosis of the epithelial cell were observed at 20°C; whereasthese were less affected at 5°C and 8°C. Lowest hemorrhages in the spleen and lowest size of sinusoids were observed in 8°C and 5°C treatments respectively. Overall, temperature variation had a superior impact on gills than spleen. Taken together, the results of the present study showed that sudden variation beyond the optimum temperature leads to physiological and pathological changes.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47689035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Validating mercury levels in catfish Netuma thalassina (Rüppell, 1837) during and aftermath ‘fish kill’ in Kuwait Bay","authors":"B. .. Thomas, A. Bu-Olayan","doi":"10.22092/IJFS.2020.122943","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122943","url":null,"abstract":"Mass mortality of Catfish Netuma thalassina during the peak summer in Kuwait Bay prompted the study of mercury concentrations using direct mercury analyzer (DMA-80) during the ‘fish kill’ otherwise, found below the detectable limits. The mean mercury (Hg) concentrations in seawater, sediment and body parts of N. thalassina (0.27 ng L-1, 0.35 ng g-1, 0.23 ng g-1) was high during ‘fish kill’ on 29th April, 2017 when compared aftermath the ‘fish kill’ (0.20 ng L-1, 0.29 ng g-1 and 0.15 ng g-1) respectively. Hg concentrations in the collected samples were observed in the sequence of Site-II>Site-I>Site-III. Fish stress was validated by hepatosomatic index (HSI). During ‘fish kill’ a decrease in liver weight due to liver shrinkage against their body weight was observed in contrast aftermath the ‘fish kill’. Mass mortality of N. thalassina was also suspected because of underwater explosion-a plan that is adopted when complex construction activities were made across the Kuwait Bay sites. Since, fish with swim bladder is susceptible to explosion, environmental variations and hazardous effluents, such factors are validated, and futuristic research delved in this line.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3151-3159"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47006776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. H. Sahafi, S. D. Madiseh, S. Koohilai, M. Hamidinejad, M. Velayatzadeh
{"title":"Plasma levels of sex steroids (testosterone, progesterone and 17β-estradiol) in Rohu Carp Labeo rohita broodstock from Khuzestan, Iran","authors":"H. H. Sahafi, S. D. Madiseh, S. Koohilai, M. Hamidinejad, M. Velayatzadeh","doi":"10.22092/IJFS.2020.122934","DOIUrl":"https://doi.org/10.22092/IJFS.2020.122934","url":null,"abstract":"Sex steroids (testosterone, progesterone and 17β-estradiol) play important roles in controlling reproduction and sexual maturity in teleosts. The aim of the present study was to evaluate the seasonal changes of these steroids in Rohu Labeo rohita. Forty gravid females and males L. rohita reared in different seasons (spring, summer, autumn, winter) were caught by a net with 1 cm mesh from earthen ponds of Aquaculture Research Institute, in south of Iran. The highest levels of 17β-estradiol (122.8±17.73 ng mL-1), testosterone (0.11±0.003 ng mL-1) and progesterone (0.86±0.01 ng mL-1) in female fish were recorded in autumn. In addition, the highest concentration of testosterone in male was determined as 0.84±0.003 ng mL-1 in autumn. The mean value of dissolved oxygen in four seasons (spring, summer, autumn, and winter) were 9.21±0.77, 8.36±0.51, 10.41±0.9 and 13.14±0.72 mg L-1, with temperature 25.47±1.07, 29.88±1.42, 20.23±0.69 and 14.63±0.47◦C, respectively. Therefore, it can be inferred that progesterone was considered as the critical index for seasonal breeding selection. There was an appropriate condition in the mid winter for broodstock selection and breeding of fish with hormones manipulation.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"19 1","pages":"3063-3074"},"PeriodicalIF":0.9,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47986185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}