Iranian Journal of Fisheries Sciences最新文献

Effect of different Cobalt (CoCl2) concentrations on cell growth, some biochemical composition, and fatty acids profile of the marine microalga Tetraselmis subcordiformis 不同浓度的钴(CoCl2)对海洋微藻四瓣藻细胞生长、部分生化组成和脂肪酸谱的影响

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-09-26 DOI: 10.22092/IJFS.2021.124953

Hossein Skandarnia, M. S. Mehrgan, H. R. Islami, M. Soltani, A. Kamali

{"title":"Effect of different Cobalt (CoCl2) concentrations on cell growth, some biochemical composition, and fatty acids profile of the marine microalga Tetraselmis subcordiformis","authors":"Hossein Skandarnia, M. S. Mehrgan, H. R. Islami, M. Soltani, A. Kamali","doi":"10.22092/IJFS.2021.124953","DOIUrl":"https://doi.org/10.22092/IJFS.2021.124953","url":null,"abstract":"The aim of this study was to determine the effects of cobalt concentration on cell growth, some biochemical composition, and fatty acids profile of the marine microalga Tetraselmis subcordiformis. Cobalt deprivations did not cause a considerable change in growth photosynthesis activity as compared to the control group. Induction of maximum lipid production was achieved using 0.001 mg L-1 of cobalt-deprived T. subcordiformis. The highest crude protein content (33.75 mg L-1) was observed in 0.001 mg L-1 cobalt. The highest and lowest lipid accumulation in T. subcordiformis was observed in 0.001 mg L-1 and 10 mg L-1 cobalt, respectively. Under cobalt deficient conditions, T. subcordiformis produced a large quantity of saturated fatty acids and the iodine and saponification value. Biodiesel characteristics were enhanced with Co+2 reduction. Also, biodiesel quality decreased with the increase of cobalt. The results showed that the maximum and minimum carotenoids were observed in 0.1 and 10 mg L-1 cobalt, respectively.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47506756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carotenoprotein from by-product of banana shrimp (Penaeus merguiensis) extracted using protease from viscera of rainbow trout: antiradical and angiotensin I-converting enzyme inhibitory activity 用虹鳟鱼内脏蛋白酶提取香蕉对虾副产物胡萝卜素蛋白:抗自由基和血管紧张素i转换酶抑制活性

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-09-01 DOI: 10.22092/IJFS.2021.350837.0

G. T. Andevari, M. Rezaei, Mehdi Tabarsa, T. Rustad

{"title":"Carotenoprotein from by-product of banana shrimp (Penaeus merguiensis) extracted using protease from viscera of rainbow trout: antiradical and angiotensin I-converting enzyme inhibitory activity","authors":"G. T. Andevari, M. Rezaei, Mehdi Tabarsa, T. Rustad","doi":"10.22092/IJFS.2021.350837.0","DOIUrl":"https://doi.org/10.22092/IJFS.2021.350837.0","url":null,"abstract":"Carotenoprotein as a marine bioactive compound was recovered from banana shrimp by-product using protease from rainbow trout viscera at various levels (5 and 15 units/g shrimp shell). The yield of carotenoprotein increased with increasing enzyme concentration up to 51.6%, which was consistent with increasing degree of hydrolysis. Protein and lipid content of the hydrolysate was 79.6 and 9.4 percent, respectively. The hydrolysate had a high nutritional value with a high amount of essential amino acids (465 mg/g) compared to the control (254 mg/g). The carotenoprotein exhibited a dose-dependent increase in antiradical activity measured by two in vitro assays, with an efficiency of 94 and 57 percent for DPPH and ABTS scavenging activities, respectively. Protease-extracted carotenoprotein showed higher ACE inhibiting effect than the control at different concentrations (p<0.05). The results from this study showed that carotenoprotein from banana shrimp have both radical scavenging activity and antihypertensive properties and a high content of essential amino acids, and could be good source for value-added nutritive food ingredients.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"1510-1525"},"PeriodicalIF":0.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48067182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Article: Population dynamics and fishery status of Trichiurus lepturus (Largehead hairtail) in the northern waters of the Oman Sea (Sistan and Baluchestan waters, Iran) 研究论文:阿曼海北部海域(伊朗锡斯坦和俾路支斯坦海域)lepturus(大头带鱼)种群动态和渔业现状

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-07-10 DOI: 10.22092/IJFS.2021.124414

S. A. T. Motlagh, S. Hashemi, M. Mirzaei

引用次数: 0

Research Article: Molecular genetic divergence of five genera of cypriniform fish in Iran assessed by DNA barcoding 研究文章:利用DNA条形码分析伊朗鲤形鱼5属的分子遗传差异

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-05-10 DOI: 10.22092/IJFS.2021.350276.0

S. Nazari, H. Paknejad, A. Jalali, Z. Khorshidi

{"title":"Research Article: Molecular genetic divergence of five genera of cypriniform fish in Iran assessed by DNA barcoding","authors":"S. Nazari, H. Paknejad, A. Jalali, Z. Khorshidi","doi":"10.22092/IJFS.2021.350276.0","DOIUrl":"https://doi.org/10.22092/IJFS.2021.350276.0","url":null,"abstract":"The present study represents a comprehensive molecular assessment of some family of freshwater fishes in Iran. We analyzed cytochrome oxidase I (COI) sequences for five genus of cypriniform fishes from Iran. The present investigation provides data on genetic structure of some species of Nemachilidae including Paraschistura bampurensis, Oxynoemacheilus kiabii and Turcinemacheilus saadii and Leuciscine cyprinids Alburnoides bipunctatus and Alburnus alburnus from inland waters of Iran. The DNA sequences of the mitochondrial COI gene in all fishes were amplified, and the resulting sequences were compared to entries in GenBank using the Basic Local Alignment Search Tool for nucleotide data. Individuals were assigned to groups using COI gene sequence divergence analysis which was determined using Kimura 2parameter distances. Results revealed two major clusters which were inconsistent characterized by mediocre genetic divergence (mean 0.8%). The sequences of P. bampurensis showed that specimens collected from four rivers (Beshar, Khersan, Shapour and Fahlian Rivers, southwest of Iran), with 1.89% for Nemacheilian loaches and 0.8% for two Leuciscine cyprinids within-species Kimura two parameters distance, shared haplotype in Neighbor-joining (NJ) tree. Some clusters showed haplotype sharing, or low levels of divergence between species, hindering reliable identification. We discussed the importance of further DNA barcode studies for native and invasive cyprinid species and subsequent submission to GenBank databases for more reliable species match and inference.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"628-645"},"PeriodicalIF":0.9,"publicationDate":"2021-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41338802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Research Article: A GIS based analysis of essential habitat for six commonly caught species in coastal creeks of Sindh, Pakistan 研究文章：基于GIS的巴基斯坦信德省沿海溪流六种常见捕获物种基本栖息地分析

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-05-10 DOI: 10.22092/IJFS.2021.124115

Faiza Sarwar, J. Kazmi, S. Shaikh

{"title":"Research Article: A GIS based analysis of essential habitat for six commonly caught species in coastal creeks of Sindh, Pakistan","authors":"Faiza Sarwar, J. Kazmi, S. Shaikh","doi":"10.22092/IJFS.2021.124115","DOIUrl":"https://doi.org/10.22092/IJFS.2021.124115","url":null,"abstract":"This study was carried out to evaluate natural resources of some Indus creeks using geospatial technologies. The first phase of the study was a detailed monthly water quality and fish stock survey for one year along with associated environmental observations, which was analysed via Landsat 8 imagery for 2014. In the second phase of the study, appropriate indicators were selected in weighted overlay on ArcGIS platform to perform creek productivity analysis. For this purpose, 13 creeks of Indus delta were selected for detailed analysis. These creeks were chosen using GIS techniques based on accessibility, depth and size. Results obtained from a total number of 65,085 fish catch from 252 identified fisheries important species in which 134 species were estuarine, 107 species marine and 11 species belonged to freshwater. Out of these, six species of Penaeus indicus, Pennahia anea, Stolephorus indicus, Escualosa thoracata, Charybdis sp. and Acanthopagrus arabicus were selected for spatial distribution analysis. It is observed that creeks of west of Jhang River known as Issaro, Waddi Khuddi, Patiani, Mal, Dabbo, Chann, Richhal had higher fish abundance and species richness than those of eastern creeks. The important fisheries species showed strong correlation with mangroves, salinity and temperatures. This study indicated that all parameters were strongly dependent on anthropogenic interventions at the study area and these activities seriously affected fish catch and growth of species mainly because of recent environmental changes. It has been revealed from the creek productivity analysis that the study area is still highly productive and intact as an","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"789-809"},"PeriodicalIF":0.9,"publicationDate":"2021-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48775267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Spirulina platensis and Azolla nilotica as feed additives on growth performance, antioxidant enzymes and fecundity of Oreochromis niloticus 螺旋藻和尼罗杜鹃饲料添加剂对尼罗褐虾生长性能、抗氧化酶和繁殖力的影响

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-05-10 DOI: 10.22092/IJFS.2021.124136

A. El-Daim, A. E. Asely, M. Kandiel, E. A. El-Gawad, Hiam Elabd, A. Shaheen, Amany Abbass

{"title":"Effect of Spirulina platensis and Azolla nilotica as feed additives on growth performance, antioxidant enzymes and fecundity of Oreochromis niloticus","authors":"A. El-Daim, A. E. Asely, M. Kandiel, E. A. El-Gawad, Hiam Elabd, A. Shaheen, Amany Abbass","doi":"10.22092/IJFS.2021.124136","DOIUrl":"https://doi.org/10.22092/IJFS.2021.124136","url":null,"abstract":"This study was conducted in two experiments. Experiment 1 aimed to investigate the growth promoting and anti-oxidative effects of Azolla nilotica (AZN) and Spirulina platensis (SP) in Oreochromis niloticus. Seven fish groups (G1-G7), each in three replicates, were fed a basal diet (control), AZN 5%, AZN 10%, SP 0.5%, SP 1%, a mixture of Azolla nilotica 5% and Spirulina platensis 1% (AZN 5 %-SP 1% mix), and a mixture of Azolla nilotica 10% and Spirulina platensis 1% (AZN 10% SP 1% mix), respectively, for 3 months. The results showed a significant increase in growth indices (weight gain, specific growth rate, average length gain, feed efficiency ratio), and a decrease in the feed conversion ratio in all supplemented groups compared to control. Hepato-somatic index in G4 and G5 groups, and intestinal-somatic index in G4 G6, and in G5 and G6 were obviously higher than control. Spleno-somatic index and antioxidant enzymes (GSH-px, SOD and CAT) markedly increased in G5 and G6 compared to control. Significant increase (p<0.05) in white blood cells was recorded in G2 and G4. Experiment 2 verified the effect of one-month supplementation of SP 1% on males and females O. niloticus fecundity. Males fed with SP 1% had an enhanced fertility as indicated by an increase in sperm density, spermatozoa motility and livability rates compared with the control. There was a significant increase in eggs number and the fecundity index in unripe females and a tendency of egg size to increase in ripen fish. It could be concluded that SP 1% and AZN 5% supplementation is advantageous to improve O. niloticus growth and fecundity.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"846-862"},"PeriodicalIF":0.9,"publicationDate":"2021-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48110233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Research Article: Determinants of fish distribution pattern and habitat preference in protected Karaj River, Iran 研究文章:伊朗卡拉吉河保护区鱼类分布格局和生境偏好的决定因素

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-03-10 DOI: 10.22092/IJFS.2021.123838

S. M. Mostafavi, M. Rahmani, Mohsen Kaboli, A. Abdoli

{"title":"Research Article: Determinants of fish distribution pattern and habitat preference in protected Karaj River, Iran","authors":"S. M. Mostafavi, M. Rahmani, Mohsen Kaboli, A. Abdoli","doi":"10.22092/IJFS.2021.123838","DOIUrl":"https://doi.org/10.22092/IJFS.2021.123838","url":null,"abstract":"Lack of information about ichthyofauna makes it difficult to predict human effects on the communities. In this study, the effects of the environmental and human factors were examined on altitudinal patterns of fish habitat selection, faunal changes, species richness and fish abundance from upstream to downstream in the southern part of the central Alborz Protected Area during 2016 and 2017. 366 fish individuals of five species in three families were identified and brown trout was the dominant species. The river was divided into three zones based on habitat structure, species composition and influence of physical and chemical variables. The results indicate that, in some situations, including small changes in habitat structure indices, physical and chemical variables along the altitudinal gradient are more effective in habitat preference, describing the composition, and abundance. Chemical variables can help to have a better understanding of the conventional patterns, shaped by the habitat structure and physical variables. Habitat suitability index model for brown trout in three zones is completely different over the two years and this species could overcome these conditions. However, upstream areas were more appropriate. Dam, with decreases of outlet water temperature, also creates homogeneous and simple habitats leading to changes in expected fish distribution patterns with decreasing altitude. These conditions may have increased habitat suitability index for the brown trout in downstream, while despite favorable ecological conditions in the middle zone, habitat suitability index and alternative habitats available in this zone are likely to decline due to density and interspecific competition. The natural pattern of fish distribution and habitat selection varies along the altitudinal gradient due to various factors and human disturbances. Optimal management of protected areas can control the effects of some of these factors at least temporarily.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"410-429"},"PeriodicalIF":0.9,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48033788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Article: Study on formulated diet for large yellow croaker (Larimichthys crocea) larvae at the early feeding stage 研究文章：大黄鱼幼虫早期饵料配方研究

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-03-10 DOI: 10.22092/IJFS.2021.123777

R. Chen, Y. Hong, K. Sun

{"title":"Research Article: Study on formulated diet for large yellow croaker (Larimichthys crocea) larvae at the early feeding stage","authors":"R. Chen, Y. Hong, K. Sun","doi":"10.22092/IJFS.2021.123777","DOIUrl":"https://doi.org/10.22092/IJFS.2021.123777","url":null,"abstract":"Most hatcheries in China rely on live prey for successful rearing of fish and shrimp larvae. Besides the high costs, live prey with pathogenic bacteria also increases the risk of contracting foodborne diseases. More than 3.5 billion large yellow croaker (Larimichthys crocea) larvae (total length over 4-centimeters) were raised in 2016. However, no formulated diet is available for replacing live feed in early stages of this croaker so far. In the present study, a new microdiet (Yq) is investigated for rearing croaker larvae at early stages (15 days after hatching). A commercial import diet (Cd) and live prey (Lp) were fed as controls. About 20,000 larvae per tank were hatched from Ningde Fufa Fishery Company in May 4th, 2017. Quality parameters, such as growth performance, survival and three digestive enzyme activities were measured. The survival rate of Yq (58.5%), Cd (51.5%) and Lp (69.6%) exhibited no significant difference (p >0.05). Furthermore, the cost of Yq was only about 23.3% of the cost of Lp. Specific growth rate in Lp was about 8 times higher (p 0.05). The high survival rate and low cost of Yq showed that it is suitable in rearing Larimichthys crocea larvae at the early stages.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"313-323"},"PeriodicalIF":0.9,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49137941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Research Article: Evaluation of Yarrowia lipolytica lipase 2 on growth performance, digestive enzyme activity and nutritional components of Russian sturgeon (Acipenser gueldenstaedtii) 研究论文:溶脂耶氏菌脂肪酶2对俄罗斯鲟鱼生长性能、消化酶活性及营养成分的影响

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-03-10 DOI: 10.22092/IJFS.2021.123835

Wen-qi Chen, Cheng-cai Zheng, Zhihong Jin, Zifeng Ye, Jun-wei Wu, Qian Shichao, Wu Zhenju, Cong Sun, Yuqiang Sun, Hui Fei

{"title":"Research Article: Evaluation of Yarrowia lipolytica lipase 2 on growth performance, digestive enzyme activity and nutritional components of Russian sturgeon (Acipenser gueldenstaedtii)","authors":"Wen-qi Chen, Cheng-cai Zheng, Zhihong Jin, Zifeng Ye, Jun-wei Wu, Qian Shichao, Wu Zhenju, Cong Sun, Yuqiang Sun, Hui Fei","doi":"10.22092/IJFS.2021.123835","DOIUrl":"https://doi.org/10.22092/IJFS.2021.123835","url":null,"abstract":"The effects of Yarrowia lipolytica lipase 2 (YLL2) on growth performance, digestive enzyme activity and muscle nutritional components of Russian sturgeon (Acipenser gueldenstaedtii) were evaluated in a 56-day feeding experiment. Four experimental groups of fish with mean weight of 4.465 g were used in the study: Control group (0-control), Group 1 (1.0 g/kg YLL2), Group 2 (1.5 g/kg YLL2) and Group 3 (2.0 g/kg YLL2), respectively, with three repetitions. Fish fed diets with YLL2 at 1.0 g/kg showed the highest growth compared with that of the other groups (p<0.05). For nutrient apparent, docosahexenoic acid (DHA) and eicosapentenoic acid (EPA) concentrations in fish muscle of group which fed with 1.0 g/kg YLL2 increased 5.05% and 7.45% respectively, compared with that of control group (p<0.05). Considering the digestive enzyme activity, lipase and protease activity in liver, spleen and intestine of G1 fish was also significantly enhanced compared with that of control group (p<0.05). While, significant increase of amylase activity in intestine was only observed in fish treated with 2.0 g/kg YLL2. The present results suggested that YLL2 (1.0 g/kg) could be used as potential diet additives for aquaculture Russian sturgeon.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"396-409"},"PeriodicalIF":0.9,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42606813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Research Article: Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding 研究文章：针对鲤鱼（Linnaeus，1758）肠道紧密连接蛋白闭塞的高亲和力多克隆抗体的鉴定

IF 0.9 4区农林科学

Iranian Journal of Fisheries Sciences Pub Date : 2021-03-01 DOI: 10.22092/IJFS.2021.123785

Junchang Feng, Xiangrui Guo, Xulu Chang, Yuru Zhang, Meng Xiaolin, Ronghua Lu, Mengyuan Huang, G. Nie, Jianxin Zhang

{"title":"Research Article: Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding","authors":"Junchang Feng, Xiangrui Guo, Xulu Chang, Yuru Zhang, Meng Xiaolin, Ronghua Lu, Mengyuan Huang, G. Nie, Jianxin Zhang","doi":"10.22092/IJFS.2021.123785","DOIUrl":"https://doi.org/10.22092/IJFS.2021.123785","url":null,"abstract":"Tight junction protein, occludin, plays an important role in intestinal health of fish. To study the function of occludin in the intestinal barrier at the protein level, a rabbit occludin polyclonal antibody was prepared against heterologously expressed Cyprinus carpio A fragment of the occludin gene containing antigenic determinants was first ligated into the pET-21a, which is an expression vector and transformed into E. coli BL21 (DE3) strain. Expression of the target fusion protein was induced by isopropyl-beta-D-thiogalactopyranoside (IPTG). The purified fusion protein was used as an antigen to immunize New Zealand long-eared rabbits (Oryctolagus cuniculus) through ear margin vein and subcutaneous injection to obtain rabbit anti-carp polyclonal antibodies against occludin. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the antibody titre, and the antibody was used to determine the distribution and expression of occludin in the intestine of carp after infection with Aeromonas hydrophila. The target fusion protein had a molecular weight of approximately 31.7 ku, the antibody titre was 2.4 × 106, and the integrity of occludin protein was lower in various intestinal segments after A. hydrophila infection. The results indicated that the prepared antibody had a high titre, affinity, and specificity and can be applied to study the expression and distribution of occludin in C. carpio. The availability of this polyclonal antibody laid the foundation for the systematic study of the intestinal barrier of Cyprinus carpio . Additionally, this polyclonal antibody could also be used for explorative studies of the biological function of occludin in other fishes.","PeriodicalId":14569,"journal":{"name":"Iranian Journal of Fisheries Sciences","volume":"20 1","pages":"343-357"},"PeriodicalIF":0.9,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49646192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0