Invasion & metastasis最新文献_第3页

Mechanisms of downregulation of transfected E-cadherin cDNA during formation of invasive tumors in syngeneic mice. 同源小鼠侵袭性肿瘤形成过程中转染E-cadherin cDNA下调的机制。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024498

A Keirsebilck, L Van Hoorde, Y Gao, G De Bruyne, E Bruyneel, P Vermassen, M Mareel, F van Roy

{"title":"Mechanisms of downregulation of transfected E-cadherin cDNA during formation of invasive tumors in syngeneic mice.","authors":"A Keirsebilck, L Van Hoorde, Y Gao, G De Bruyne, E Bruyneel, P Vermassen, M Mareel, F van Roy","doi":"10.1159/000024498","DOIUrl":"https://doi.org/10.1159/000024498","url":null,"abstract":"Loss of E-cadherin expression has been observed both in experimental tumors and in human cancers and is related to invasiveness and poor differentiation. The E-cadherin-negative mouse mesenchymal tumor cell line MO4 was transfected with several plasmids expressing mouse E-cadherin cDNA. These plasmids differed from each other by the extent of E-cadherin-specific 3' untranslated region (UTR) sequences and by the use of different constitutive promoters. Transfectants were isolated that expressed functional E-cadherin in a homogeneous way. In syngeneic mice, such MO4-Ecad transfectants invariably produced malignant fibrosarcoma-like tumors, which were completely E-cadherin-negative at the protein level. Northern blotting revealed that E-cadherin mRNA expression was downregulated in some but not all MO4-Ecad tumors. Downregulation was caused by mRNA instability triggered by particular 3' UTR sequences. This in vivo downregulation of E-cadherin in malignant MO4-Ecad tumors turned out to be reversible and is likely to be mediated by host factors to be further identified.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 1","pages":"44-56"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21078271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Invasion strategies of the oral pathogen porphyromonas gingivalis: implications for cardiovascular disease. 口腔病原体牙龈卟啉单胞菌的侵袭策略:对心血管疾病的影响。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024499

R G Deshpande, M Khan, C A Genco

{"title":"Invasion strategies of the oral pathogen porphyromonas gingivalis: implications for cardiovascular disease.","authors":"R G Deshpande, M Khan, C A Genco","doi":"10.1159/000024499","DOIUrl":"https://doi.org/10.1159/000024499","url":null,"abstract":"Microorganisms have evolved a variety of mechanisms designed to evade detection and/or destruction by the host. Many pathogens evade host defenses by invading cells, thus providing the bacterium with an environment free of competing microorganisms. Adherence and invasion are active processes in which microorganisms often use host proteins and enzymes to gain entry into the cell, thus stimulating their own uptake. The investigation of invasion by the periopathogen Porphyromonas gingivalis is in its infancy in comparison with that of the enteric pathogens. However, recent studies with P. gingivalis have revealed that these organisms have developed invasion strategies and mechanisms similar to those of the enteric pathogens for both epithelial and endothelial cells. The study and elucidation of the mechanisms by which microorganisms such as P. gingivalis persist in chronic infection will provide valuable insight into the pathogenesis of P. gingivalis-mediated periodontal disease. The ability to multiply in and to activate endothelial cells may be one of the pathogenic mechanisms exerted by P. gingivalis that may explain the recently described association between this organism and cardiovascular disease.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 2","pages":"57-69"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024499","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21233470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96

Increased tumorigenicity and invasiveness of C6 rat glioma cells transfected with the human alpha-2,8 sialyltransferase cDNA. 转染人α -2,8唾液基转移酶cDNA的C6大鼠胶质瘤细胞的致瘤性和侵袭性增强

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024507

E Sottocornola, I Colombo, V Vergani, G Taraboletti, B Berra

{"title":"Increased tumorigenicity and invasiveness of C6 rat glioma cells transfected with the human alpha-2,8 sialyltransferase cDNA.","authors":"E Sottocornola, I Colombo, V Vergani, G Taraboletti, B Berra","doi":"10.1159/000024507","DOIUrl":"https://doi.org/10.1159/000024507","url":null,"abstract":"Gangliosides are thought to be involved in tumor cell proliferation, migration and invasiveness as so far demonstrated by the addition of exogenous gangliosides to the culture medium. To better understand the direct influence that alterations in ganglioside synthesis can exert on these functional aspects of cell biology, in the present study, we investigated the behaviour of C6 rat glioma cells after stable transfection with the human CMP-NeuAc:NeuAcalpha2-3Galbeta1-4GlcCer alpha2,8-sialyltransferase (SAT-II, EC 2.4.99.8) gene. The enzyme synthesizes ganglioside GD(3) by adding a sialic acid residue to ganglioside GM(3). Stable transfection of the constructs into C6 cells and expression of the human SAT-II gene were evaluated using PCR and RT-PCR amplification, respectively. Qualitative and quantitative analysis of the ganglioside profile was performed by conventional HP-TLC and identity of de novo synthesized species was assessed by TLC immunostaining. Results show that whereas C6 parental cells and C6 cells transfected with the empty expression vector synthesize, almost exclusively, ganglioside GM(3), de novo synthesis of GD(3) is clearly observed in clones expressing the alpha2,8-sialyltransferase. Subcutaneous grafting in athymic nude mice of cells expressing high levels of GD(3) induces tumors growing faster and more aggressively than controls. In in vitro assays, the same cells demonstrate increased proliferation rate, motility and invasiveness. Chemotaxis and chemoinvasion were assayed using the modified Boyden chamber. Data obtained suggest that endogenously neosynthesized GD(3) is able to modify proliferation rate, motility and invasion of C6 rat glioma cells, enhancing the features of malignancy of this tumor cell line.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 3","pages":"142-54"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Effects of EGF and TGF-alpha on invasion and proteinase expression of uterine cervical adenocarcinoma OMC-4 cells. EGF和tgf - α对子宫颈腺癌OMC-4细胞侵袭及蛋白酶表达的影响

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024510

M Ueda, H Fujii, K Yoshizawa, Y Terai, K Kumagai, K Ueki, M Ueki

{"title":"Effects of EGF and TGF-alpha on invasion and proteinase expression of uterine cervical adenocarcinoma OMC-4 cells.","authors":"M Ueda, H Fujii, K Yoshizawa, Y Terai, K Kumagai, K Ueki, M Ueki","doi":"10.1159/000024510","DOIUrl":"https://doi.org/10.1159/000024510","url":null,"abstract":"Uterine cervical adenocarcinoma typically is an aggressive neoplasm with a propensity for early invasion and dissemination; however, the regulatory mechanism of invasive activity of cervical adenocarcinoma cells has not been fully understood. In this study, biological effects of epidermal growth factor (EGF) and transforming growth factor (TGF)-alpha on invasion and proteinase expression of human cervical adenocarcinoma OMC-4 cells were investigated. Tumor cell migration along a gradient of substratum-bound fibronectin and invasion into the reconstituted basement membrane were stimulated by 0.1-10 nM EGF and TGF-alpha in a concentration-dependent manner. Their effects on tumor cell migration were also confirmed by wound assay. The zymography of tumor-conditioned medium showed that the treatment of OMC-4 cells with EGF and TGF-alpha resulted in the increase of matrix metalloproteinase (MMP)-2 and urokinase-type plasminogen activator (uPA). Matrilysin (MMP-7), also secreted by OMC-4 cells, was not affected by these growth factors. These results suggest that EGF and TGF-alpha act as positive regulators on the invasion of cervical adenocarcinoma cells, which may be associated with their stimulatory effects on tumor cell motility and the induction of type IV collagenase and uPA secreted by tumor cells.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 4","pages":"176-83"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024510","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21496232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Establishment of high and low metastasis cell lines derived from a human tongue squamous cell carcinoma. 人舌鳞状细胞癌高、低转移细胞系的建立。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024515

S Nakayama, A Sasaki, H Mese, R E Alcalde, T Matsumura

引用次数: 49

Maximum effect of urokinase plasminogen activator inhibitors in the control of invasion and metastasis of rat mammary cancer. 尿激酶纤溶酶原激活物抑制剂在控制大鼠乳腺癌侵袭转移中的最大作用。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024518

D M Evans, K D Sloan-Stakleff

引用次数: 18

Serum obtained from rats after partial hepatectomy enhances growth of cultured colon carcinoma cells. 大鼠肝部分切除后血清可促进培养结肠癌细胞的生长。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024508

K P de Jong, M A Brouwers, M L van Veen, M Brinker, E G de Vries, T Daemen, G L Scherphof, M J Slooff

引用次数: 12

c-K-ras overexpression is characteristic for metastases derived from a methylcholanthrene-induced fibrosarcoma. c-K-ras过表达是甲基胆碱诱导的纤维肉瘤转移灶的特征。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024519

I Algarra, M Perez, M J Serrano, F Garrido, J J Gaforio

{"title":"c-K-ras overexpression is characteristic for metastases derived from a methylcholanthrene-induced fibrosarcoma.","authors":"I Algarra, M Perez, M J Serrano, F Garrido, J J Gaforio","doi":"10.1159/000024519","DOIUrl":"https://doi.org/10.1159/000024519","url":null,"abstract":"We investigated the relationship between the activation of the c-myc and c-K-ras proto-oncogenes and the acquisition of metastatic potential in a methylcholanthrene-induced BALB/c fibrosarcoma. The murine fibrosarcoma GR9 was originally induced in BALB/c mice following exposure to the carcinogenic chemical 3-methylcholanthrene. To induce spontaneous metastasis, we used two tumor cell clones (B9 and G2) known to differ in their metastatic potential, local tumor growth, H-2 class I expression and sensitivity to natural killer (NK) cells. The metastatic nodes were obtained from the lung, liver and kidney. The results showed: (1) amplification of the c-myc proto-oncogene in original tumor clones as well as in all metastatic nodes; (2) mRNA overexpression without amplification of the K-ras proto-oncogene in the metastatic cells, regardless of their anatomical location; (3) no c-K-ras point mutations at codons 12 and 61, and (4) in general, a statistically significantly reduced in vitro sensitivity of metastatic tumor cells to NK cells as compared with the tumor clones used to induce them (p<0.05). These results therefore suggest that overexpressed c-K-ras mRNA is important during tumor progression, perhaps rendering metastatic tumor cells more resistant to lysis by NK cells.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 5-6","pages":"261-70"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024519","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21580839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Truncated dipeptidyl peptidase IV is a potent anti-adhesion and anti-metastasis peptide for rat breast cancer cells. 截断二肽基肽酶IV是一种有效的抗乳腺癌细胞粘附和转移肽。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024497

M Abdel-Ghany, H Cheng, R A Levine, B U Pauli

引用次数: 31

Human acellular dermal matrix as a novel model of malignant epithelial cell invasion. 人脱细胞真皮基质作为恶性上皮细胞侵袭的新模型。

Invasion & metastasis Pub Date : 1997-01-01

K M Bullard, M J Banda, J M Arbeit, E Bergsland, D M Young

{"title":"Human acellular dermal matrix as a novel model of malignant epithelial cell invasion.","authors":"K M Bullard, M J Banda, J M Arbeit, E Bergsland, D M Young","doi":"","DOIUrl":"","url":null,"abstract":"Cancer invasion and metastasis are associated with matrix degradation. We describe a novel in vivo model of invasion by squamous epithelial neoplastic cells derived from transgenic mice grown on acellular human dermis. Human dermis was subjected to multiple freeze-thaw cycles to render it acellular, maintaining the basement membrane of the former dermal-epidermal junction. Cells representing discrete stages of a multistep transgenic mouse model of epidermal carcinogenesis (neonatal transgenic keratinocytes, moderately/poorly differentiated squamous cell carcinoma, and lymph node metastasis) were seeded onto the basement membrane surface, grown in culture for 4 days, grafted in a subpannicular pocket of athymic mice, and harvested after 3 weeks. Histological analysis demonstrated that neonatal transgenic keratinocytes did not degrade the basement membrane or invade the underlying dermis. In contrast, malignant cells derived from both a moderately differentiated squamous carcinoma and a lymph node metastasis were highly invasive. Immunohistochemical analysis revealed collagenase only in nests of invading malignant cells in contact with the dermal matrix, but not in the tumor mass remaining above the basement membrane, suggesting that this proteinase may be required for stromal invasion. This novel model recapitulates the events seen in malignant invasion: transgenic keratinocytes are unable to penetrate the dermis while cells from a moderately differentiated carcinoma and from lymph node metastasis consistently invade.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 1","pages":"42-52"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20352278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0