Invasion & metastasis最新文献_第4页

Bacterium-assisted invasion of Entamoeba histolytica through human enteric epithelia in two-compartment chambers. 细菌辅助溶组织内阿米巴通过人肠上皮在双室室侵袭。

Invasion & metastasis Pub Date : 1997-01-01

A Leroy, G De Bruyne, A Verspeelt, T Lauwaet, H Nelis, M Mareel

引用次数: 0

The role of osteopontin in tumorigenesis and metastasis. 骨桥蛋白在肿瘤发生和转移中的作用。

Invasion & metastasis Pub Date : 1997-01-01

A J Oates, R Barraclough, P S Rudland

引用次数: 0

Expression of E-cadherin and alpha-,beta- and gamma-catenins in human bladder carcinomas: are they good prognostic factors? e -钙粘蛋白和α -、β -和γ -连环蛋白在人膀胱癌中的表达:它们是良好的预后因素吗?

Invasion & metastasis Pub Date : 1997-01-01

A Mialhe, J Louis, S Montlevier, M Peoch, D Pasquier, J L Bosson, J J Rambeaud, D Seigneurin

{"title":"Expression of E-cadherin and alpha-,beta- and gamma-catenins in human bladder carcinomas: are they good prognostic factors?","authors":"A Mialhe, J Louis, S Montlevier, M Peoch, D Pasquier, J L Bosson, J J Rambeaud, D Seigneurin","doi":"","DOIUrl":"","url":null,"abstract":"E-cadherin, the epithelium-specific cadherin, is known to play a major role in tumor progression in many human carcinomas, via intercellular homophilic Ca2+-dependent adhesion. This adhesion is mediated by a group of cytoplasmic proteins, including the alpha-, beta- and gamma-catenins that link the E-cadherin to the actin cytoskeleton. Recent studies have shown that loss or reduction of either E-cadherin or catenin expression was strictly related to clinicopathological data in bladder tumors, and E-cadherin might constitute prognostic factors in bladder carcinogenesis. Here we continued a preliminary work on E-cadherin in bladder cancer. In an effort to evaluate their possible prognostic value, we investigated both E-cadherin and catenins in 99 bladder tumors by immunohistochemistry. E-cadherin and all the catenins were strongly expressed in normal urothelium. Regarding histopathological data, the tumors examined showed that the disrupted expression of each molecule, except for gamma-catenin, was directly related to increasing tumor grade (mainly for alpha- and beta-catenin) and deep invasion (p < or = 0.01). The aberrant expression of E-cadherin and beta-catenin was also correlated to the presence of distant metastasis (p < 0.05). However, only abnormal expression of a-catenin was associated with poor survival (p = 0.037). Therefore our results suggest that alpha-catenin is directly involved in tumor invasion and dedifferentiation and is the only protein of any prognostic value, albeit low in patients with bladder cancer.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 3","pages":"124-37"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20619131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunophenotype of human HT29 colon cancer cell metastases in the lungs of scid mice: spontaneous versus artificial metastases. 人HT29结肠癌细胞在scid小鼠肺转移的免疫表型:自发与人工转移。

Invasion & metastasis Pub Date : 1997-01-01

B S Mitchell, H P Horny, E Adam, U Schumacher

{"title":"Immunophenotype of human HT29 colon cancer cell metastases in the lungs of scid mice: spontaneous versus artificial metastases.","authors":"B S Mitchell, H P Horny, E Adam, U Schumacher","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to compare the immunophenotype of the human colon cancer cell line HT29 tumour deposits in the lung which occurred spontaneously after subcutaneous implantation with those which arose after intravenous injection into severe combined immunodeficient (scid) mice. Irrespective of the route of implantation the colon cancer cells were readily observed in the lungs of the scid mice. Similar patterns of immunoreactivity for the proliferative markers (MiB-1, PCNA), and for the tumour suppressor gene (p53) were detected in both groups, and for carcinoembryonic antigen, with only minor quantitative differences in levels of marker expression. Whereas the marker CD44 variant 6 gave very little reaction after either route, cytokeratin expression varied amongst the different cytokeratins (CK 7, 18 or 20), and with the route of implantation. CA125 and E-cadherin were weakly expressed after intravenous injection, but generally not after subcutaneous implantation. Vimentin was not demonstrated in any of the specimens examined. In general, the expression of proliferative markers, and of oncogenes, appears to be independent of the implantation route, whilst expression of cell adhesion molecules can be dependent on the route of implantation.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 2","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20481385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship of epidermal growth factor binding capacity to histopathologic features and prognosis in human renal cell carcinoma. 人肾细胞癌表皮生长因子结合能力与组织病理特征及预后的关系。

Invasion & metastasis Pub Date : 1997-01-01

S Sakamoto, S Kitahara, S Sumi, S Horiuchi, K Yoshida

引用次数: 0

Signal transduction pathway of the induction of cell motility in hamster pancreatic ductal adenocarcinoma cell. 仓鼠胰腺导管腺癌细胞诱导细胞运动的信号转导途径。

Invasion & metastasis Pub Date : 1997-01-01

J Akagi, H Egami, T Kurizaki, H Ohmachi, M Ogawa

{"title":"Signal transduction pathway of the induction of cell motility in hamster pancreatic ductal adenocarcinoma cell.","authors":"J Akagi, H Egami, T Kurizaki, H Ohmachi, M Ogawa","doi":"","DOIUrl":"","url":null,"abstract":"Recently, we reported that low (PC-1)- and high-invasive cell lines (PC-1.0) were established on the basis of hamster pancreatic ductal adenocarcinomas, and PC-1.0 cells were secreting the dissociation factor in the supernatant (DF-CM) which induced cell dissociation and enhancement of cell motility. The cell motility of PC-1.0 is about 6 times as high as that of PC-1, which was continuously maintained in an autocrine fashion by DF-CM. In contrast, cell motility of PC-1 was rapidly induced by DF-CM with a high level of induction of endogenous c-fos mRNA and returned to a basal level within 6 h. The inhibition experiment using antisense oligonucleotides to c-fos indicated that the high level of induction of c-fos mRNA observed in the DF-CM-treated PC-1 cells was closely associated with their induction of cell motility. To elucidate these differences of responses against DF-CM between PC-1 and PC-1.0, signal transduction pathways of induction of the cell motilities were analyzed, using protein kinase C (PKC) inhibitor, 12-O-tetradecanoylphorbol-13-acetate, cyclic AMP antagonist, and cyclic AMP agonist. The transiently enhanced cell motility of DF-CM-treated PC-1 cells was completely inhibited by the cyclic AMP antagonist, and the cyclic AMP agonist was able to induce a similar pattern of induction of cell motility in PC-1 cells to DF-CM. On the other hand, the highly enhanced cell motility of PC-1.0 was completely inhibited by protein kinase C inhibitor, but not by cyclic AMP antagonist. These results suggest that cell motility of low-invasive PC-1 cells is under control through cyclic AMP-dependent protein kinase A, while the protein kinase C pathway seems favorable for high-invasive PC-1.0 cells to maintain the continuously enhanced cell motility responsible for their high invasiveness.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 1","pages":"16-25"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20352276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential responsiveness of murine T lymphomas to local growth and invasion factors may determine metastasis formation in the ovaries. 小鼠T淋巴瘤对局部生长和侵袭因子的不同反应可能决定卵巢转移的形成。

Invasion & metastasis Pub Date : 1997-01-01

C Schmidt, A Laporte, P De Baetselier

{"title":"Differential responsiveness of murine T lymphomas to local growth and invasion factors may determine metastasis formation in the ovaries.","authors":"C Schmidt, A Laporte, P De Baetselier","doi":"","DOIUrl":"","url":null,"abstract":"The murine T cell hybridomas BW-14 and BW-19, both derived from a fusion between the nonmetastatic BW 5147 lymphoma and a cytotoxic T cell line, differ in their capacity to metastasize to the ovaries. While ovary colonization by BW-19 cells is marginal and limited to the ovary follicles, BW-14 cells extensively colonize the complete ovary. The present study shows that the two T cell hybridomas respond differentially to ovary-derived migration and growth-modulating factors, in a way that correlates with their differential capacity to metastasize to the ovaries. More specifically, we observed that conditioned medium from cultured ovary fragments or from the ovary-derived granulosa cell line GRMO1V inhibited the migration of BW-19 cells in vitro, but stimulated the migration of BW-14 cells. Likewise, the local hormone prostaglandin E2 (PGE2) and the steroid hormone progesterone, both known to be secreted by GRMO1V cells, stimulated the migration of BW-14 cells, indicating that the stimulatory effect of the conditioned medium can be at least partially ascribed to the action of these two hormones. In contrast, the migration of BW-19 cells was inhibited by PGE2. In addition to the modulatory effect on hybridoma cell migration, conditioned medium from the granulosa cell line GRMO1V inhibited the proliferation of BW-19 cells in vitro, an effect that is likely to be mediated at least partially by PGE2. The proliferation of BW-14 cells, on the other hand was, depending on the dilution used, stimulated or inhibited by GRMO1V-conditioned medium. Our findings indicate that the differential capacity of the T cell hybridomas BW-14 and BW-19 to metastasize to the ovaries is mediated by the differential action of granulosa-cell-derived factors, in particular the sex hormone progesterone and the local hormone PGE2, on both the migration and proliferation of the T cell hybridomas.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 2","pages":"53-61"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20481381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral squamous cell carcinoma invasion is associated with a laminin-5 matrix re-organization but independent of basement membrane and hemidesmosome formation. clues from an in vitro invasion model. 口腔鳞状细胞癌的侵袭与层粘连蛋白-5基质重组有关，但与基底膜和半膜小体的形成无关。来自体外侵袭模型的线索。

Invasion & metastasis Pub Date : 1997-01-01

A Berndt, P Hyckel, A Könneker, D Katenkamp, H Kosmehl

{"title":"Oral squamous cell carcinoma invasion is associated with a laminin-5 matrix re-organization but independent of basement membrane and hemidesmosome formation. clues from an in vitro invasion model.","authors":"A Berndt, P Hyckel, A Könneker, D Katenkamp, H Kosmehl","doi":"","DOIUrl":"","url":null,"abstract":"The basement membrane (BM) molecule laminin-5 represents the major component of the anchoring filaments, the BM counterpart of the hemidesmosomes. Because laminin-5 is abundantly deposited adjacent to budding invasive carcinoma cells in vivo, a special invasion-promoting role is suspected. We present a 3D collagen type I gel invasion model for oral squamous cell carcinoma (OSCC) using the newly established OSCC cell lines PE/CA-PJ15, PE/CA-PJ34, and PE/CA-PJ41. The carcinoma cells on the surface of the collagen type I gel show an invasive growth exclusively in the presence of gel-inoculated (myo)fibroblasts yielded from nodular palmar fibromatosis or tumour stroma. The model is characterized by an immunohistochemical and ultrastrcutural failure of structural BM and hemidesmosome formation. In the model an invasion-associated modulation of the laminin-5 deposition (alpha3, clone BM165; gamma2, clone GB3) was demonstrated: in invasive areas a strong ribbon-like immunostaining in the tumor-gel interface. The results obtained from the invasion model suggest that the assumed invasion-promoting ability of the BM molecule laminin-5 is realized independent of a structural BM and hemidesmosome formation.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 5","pages":"251-8"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20784005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of tumor cell motility by plasmin. 纤溶酶对肿瘤细胞运动的调节。

Invasion & metastasis Pub Date : 1997-01-01

L Chikahisa, K Matsuo, Y Yamada

引用次数: 0

Alpha-melanocyte-stimulating hormone blocks invasion of reconstituted basement membrane (Matrigel) by murine B16 melanoma cells. α -黑色素细胞刺激激素阻断小鼠B16黑色素瘤细胞对重建基底膜(基质)的侵袭。

Invasion & metastasis Pub Date : 1997-01-01

J Murata, K Ayukawa, M Ogasawara, H Fujii, I Saiki

{"title":"Alpha-melanocyte-stimulating hormone blocks invasion of reconstituted basement membrane (Matrigel) by murine B16 melanoma cells.","authors":"J Murata, K Ayukawa, M Ogasawara, H Fujii, I Saiki","doi":"","DOIUrl":"","url":null,"abstract":"We have examined the effect of alpha-melanocyte-stimulating hormone (alpha-MSH) on invasive ability of murine melanoma cell lines with different metastatic potential in a Matrigel invasion assay. alpha-MSH potently blocked the invasion of B16-BL6 cells with highly metastatic potential in a concentration-dependent manner, whereas it was less effective in inhibiting the invasion of weakly metastatic B16-F1 cells. Pretreatment of B16-BL6 cells with alpha-MSH resulted in a decrease of the adhesiveness to fibronectin and laminin substrates in a time-dependent fashion. As assessed by zymographic analysis, alpha-MSH partially inhibited the production of matrix metalloproteinase (MMP)-2 and -9 from both cell lines to a similar degree without affecting the degradative activity of these MMPs. alpha-MSH was more potent in inhibiting the migration of B16-BL6 cells towards both fibronectin- and laminin-coated substrates than that of B16-F1 cells. The growth and morphology of B16-BL6 cells were not changed after a 7-day incubation with alpha-MSH. The number of lung tumor colonies markedly decreased when B16-BL6 cells were coinjected intravenously with 10(-6) M alpha-MSH. However, alpha-MSH had no effect on the experimental lung metastases by B16-F1 cells. These results suggest that alpha-MSH suppressed the invasive and metastatic properties of B16 melanoma cells, and the degree of inhibition was associated with metastatic potential of B16 melanoma cells.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 2","pages":"82-93"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20481387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0