{"title":"小鼠T淋巴瘤对局部生长和侵袭因子的不同反应可能决定卵巢转移的形成。","authors":"C Schmidt, A Laporte, P De Baetselier","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The murine T cell hybridomas BW-14 and BW-19, both derived from a fusion between the nonmetastatic BW 5147 lymphoma and a cytotoxic T cell line, differ in their capacity to metastasize to the ovaries. While ovary colonization by BW-19 cells is marginal and limited to the ovary follicles, BW-14 cells extensively colonize the complete ovary. The present study shows that the two T cell hybridomas respond differentially to ovary-derived migration and growth-modulating factors, in a way that correlates with their differential capacity to metastasize to the ovaries. More specifically, we observed that conditioned medium from cultured ovary fragments or from the ovary-derived granulosa cell line GRMO1V inhibited the migration of BW-19 cells in vitro, but stimulated the migration of BW-14 cells. Likewise, the local hormone prostaglandin E2 (PGE2) and the steroid hormone progesterone, both known to be secreted by GRMO1V cells, stimulated the migration of BW-14 cells, indicating that the stimulatory effect of the conditioned medium can be at least partially ascribed to the action of these two hormones. In contrast, the migration of BW-19 cells was inhibited by PGE2. In addition to the modulatory effect on hybridoma cell migration, conditioned medium from the granulosa cell line GRMO1V inhibited the proliferation of BW-19 cells in vitro, an effect that is likely to be mediated at least partially by PGE2. The proliferation of BW-14 cells, on the other hand was, depending on the dilution used, stimulated or inhibited by GRMO1V-conditioned medium. Our findings indicate that the differential capacity of the T cell hybridomas BW-14 and BW-19 to metastasize to the ovaries is mediated by the differential action of granulosa-cell-derived factors, in particular the sex hormone progesterone and the local hormone PGE2, on both the migration and proliferation of the T cell hybridomas.</p>","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"17 2","pages":"53-61"},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential responsiveness of murine T lymphomas to local growth and invasion factors may determine metastasis formation in the ovaries.\",\"authors\":\"C Schmidt, A Laporte, P De Baetselier\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The murine T cell hybridomas BW-14 and BW-19, both derived from a fusion between the nonmetastatic BW 5147 lymphoma and a cytotoxic T cell line, differ in their capacity to metastasize to the ovaries. While ovary colonization by BW-19 cells is marginal and limited to the ovary follicles, BW-14 cells extensively colonize the complete ovary. The present study shows that the two T cell hybridomas respond differentially to ovary-derived migration and growth-modulating factors, in a way that correlates with their differential capacity to metastasize to the ovaries. More specifically, we observed that conditioned medium from cultured ovary fragments or from the ovary-derived granulosa cell line GRMO1V inhibited the migration of BW-19 cells in vitro, but stimulated the migration of BW-14 cells. Likewise, the local hormone prostaglandin E2 (PGE2) and the steroid hormone progesterone, both known to be secreted by GRMO1V cells, stimulated the migration of BW-14 cells, indicating that the stimulatory effect of the conditioned medium can be at least partially ascribed to the action of these two hormones. In contrast, the migration of BW-19 cells was inhibited by PGE2. In addition to the modulatory effect on hybridoma cell migration, conditioned medium from the granulosa cell line GRMO1V inhibited the proliferation of BW-19 cells in vitro, an effect that is likely to be mediated at least partially by PGE2. The proliferation of BW-14 cells, on the other hand was, depending on the dilution used, stimulated or inhibited by GRMO1V-conditioned medium. Our findings indicate that the differential capacity of the T cell hybridomas BW-14 and BW-19 to metastasize to the ovaries is mediated by the differential action of granulosa-cell-derived factors, in particular the sex hormone progesterone and the local hormone PGE2, on both the migration and proliferation of the T cell hybridomas.</p>\",\"PeriodicalId\":14452,\"journal\":{\"name\":\"Invasion & metastasis\",\"volume\":\"17 2\",\"pages\":\"53-61\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Invasion & metastasis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Invasion & metastasis","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Differential responsiveness of murine T lymphomas to local growth and invasion factors may determine metastasis formation in the ovaries.
The murine T cell hybridomas BW-14 and BW-19, both derived from a fusion between the nonmetastatic BW 5147 lymphoma and a cytotoxic T cell line, differ in their capacity to metastasize to the ovaries. While ovary colonization by BW-19 cells is marginal and limited to the ovary follicles, BW-14 cells extensively colonize the complete ovary. The present study shows that the two T cell hybridomas respond differentially to ovary-derived migration and growth-modulating factors, in a way that correlates with their differential capacity to metastasize to the ovaries. More specifically, we observed that conditioned medium from cultured ovary fragments or from the ovary-derived granulosa cell line GRMO1V inhibited the migration of BW-19 cells in vitro, but stimulated the migration of BW-14 cells. Likewise, the local hormone prostaglandin E2 (PGE2) and the steroid hormone progesterone, both known to be secreted by GRMO1V cells, stimulated the migration of BW-14 cells, indicating that the stimulatory effect of the conditioned medium can be at least partially ascribed to the action of these two hormones. In contrast, the migration of BW-19 cells was inhibited by PGE2. In addition to the modulatory effect on hybridoma cell migration, conditioned medium from the granulosa cell line GRMO1V inhibited the proliferation of BW-19 cells in vitro, an effect that is likely to be mediated at least partially by PGE2. The proliferation of BW-14 cells, on the other hand was, depending on the dilution used, stimulated or inhibited by GRMO1V-conditioned medium. Our findings indicate that the differential capacity of the T cell hybridomas BW-14 and BW-19 to metastasize to the ovaries is mediated by the differential action of granulosa-cell-derived factors, in particular the sex hormone progesterone and the local hormone PGE2, on both the migration and proliferation of the T cell hybridomas.