α -黑色素细胞刺激激素阻断小鼠B16黑色素瘤细胞对重建基底膜(基质)的侵袭。

Invasion & metastasis Pub Date : 1997-01-01
J Murata, K Ayukawa, M Ogasawara, H Fujii, I Saiki
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引用次数: 0

摘要

我们在Matrigel侵袭试验中检测了α -黑色素细胞刺激激素(α - msh)对具有不同转移潜力的小鼠黑色素瘤细胞系侵袭能力的影响。α - msh对具有高转移潜力的B16-BL6细胞的侵袭具有浓度依赖性,而对弱转移的B16-F1细胞的侵袭抑制作用较弱。用α - msh预处理B16-BL6细胞导致其对纤维连接蛋白和层粘连蛋白底物的粘附性呈时间依赖性下降。酶谱分析表明,α - msh部分抑制了两种细胞系中基质金属蛋白酶(MMP)-2和-9的产生,程度相似,但不影响这些基质金属蛋白酶的降解活性。与B16-F1细胞相比,α - msh对B16-BL6细胞向纤维连接蛋白和层粘连蛋白包被底物迁移的抑制作用更强。α - msh作用7天后,B16-BL6细胞的生长和形态未见明显变化。10(-6) M α - msh同时静脉注射B16-BL6细胞后,肺肿瘤菌落数量明显减少。然而,α - msh对B16-F1细胞的实验性肺转移没有影响。这些结果表明,α - msh抑制B16黑色素瘤细胞的侵袭和转移特性,并且抑制程度与B16黑色素瘤细胞的转移潜能有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alpha-melanocyte-stimulating hormone blocks invasion of reconstituted basement membrane (Matrigel) by murine B16 melanoma cells.

We have examined the effect of alpha-melanocyte-stimulating hormone (alpha-MSH) on invasive ability of murine melanoma cell lines with different metastatic potential in a Matrigel invasion assay. alpha-MSH potently blocked the invasion of B16-BL6 cells with highly metastatic potential in a concentration-dependent manner, whereas it was less effective in inhibiting the invasion of weakly metastatic B16-F1 cells. Pretreatment of B16-BL6 cells with alpha-MSH resulted in a decrease of the adhesiveness to fibronectin and laminin substrates in a time-dependent fashion. As assessed by zymographic analysis, alpha-MSH partially inhibited the production of matrix metalloproteinase (MMP)-2 and -9 from both cell lines to a similar degree without affecting the degradative activity of these MMPs. alpha-MSH was more potent in inhibiting the migration of B16-BL6 cells towards both fibronectin- and laminin-coated substrates than that of B16-F1 cells. The growth and morphology of B16-BL6 cells were not changed after a 7-day incubation with alpha-MSH. The number of lung tumor colonies markedly decreased when B16-BL6 cells were coinjected intravenously with 10(-6) M alpha-MSH. However, alpha-MSH had no effect on the experimental lung metastases by B16-F1 cells. These results suggest that alpha-MSH suppressed the invasive and metastatic properties of B16 melanoma cells, and the degree of inhibition was associated with metastatic potential of B16 melanoma cells.

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