Establishment of high and low metastasis cell lines derived from a human tongue squamous cell carcinoma.

S Nakayama, A Sasaki, H Mese, R E Alcalde, T Matsumura
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引用次数: 49

Abstract

Malignant tumors are composed of cells with different phenotypic properties and only certain cell subpopulations present metastatic potential. The establishment of cell lines with high or low metastatic potential is necessary to investigate the molecular mechanisms of the metastatic process. However, human oral squamous cell carcinoma (SCC) cell lines that are suitable for the above investigation are scarce. High and low metastatic cells were obtained from a primary lesion of a patient with tongue carcinoma who had not received any therapy. Two distinct cell lines were selected, UM1 with a scattered growth pattern and loose cell-cell adhesion, and UM2 with a colony-formed growth pattern and firm cell-cell adhesion. The expression of E-cadherin in UM1 was clearly lower than that in UM2. UM1 exhibited a higher motility, invasive and metastatic activity than UM2 in vivo and in vitro. A low invasive and a metastatic oral SCC cell line, useful to investigate invasion and metastasis mechanisms, have been established.

人舌鳞状细胞癌高、低转移细胞系的建立。
恶性肿瘤是由具有不同表型特性的细胞组成的,只有某些细胞亚群具有转移潜力。建立具有高或低转移潜能的细胞系是研究转移过程分子机制的必要条件。然而,适合上述研究的人口腔鳞状细胞癌(SCC)细胞系很少。高转移细胞和低转移细胞从原发病变的舌癌患者谁没有接受任何治疗。选择两个不同的细胞系,UM1具有分散的生长模式和松散的细胞-细胞粘附,UM2具有集落形成的生长模式和牢固的细胞-细胞粘附。E-cadherin在UM1中的表达明显低于UM2。UM1在体内和体外均表现出比UM2更高的运动性、侵袭性和转移性。一种低侵袭性和转移性的口腔鳞状细胞癌细胞系已经建立,有助于研究其侵袭和转移机制。
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