Invasion & metastasis最新文献_第2页

Differential expression of alphav integrins in K1735 melanoma cells. α - v整合素在K1735黑色素瘤细胞中的差异表达

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024494

X Li, B Chen, S D Blystone, K P McHugh, F P Ross, D M Ramos

{"title":"Differential expression of alphav integrins in K1735 melanoma cells.","authors":"X Li, B Chen, S D Blystone, K P McHugh, F P Ross, D M Ramos","doi":"10.1159/000024494","DOIUrl":"https://doi.org/10.1159/000024494","url":null,"abstract":"Tumor cell adherence to and migration on the extracellular matrix is an important aspect of cancer progression. This interaction with the extracellular matrix is mediated primarily through the integrin class of cell adhesion molecules. We identified a restricted expression of alphavbeta3 in highly metastatic K1735M2 and of alphavbeta5 in poorly metastatic K1735C23 murine melanoma cells. The highly metastatic cells were ten times more motile on vitronectin and fibronectin and approximately three times more invasive through a reconstituted basement membrane than the poorly metastatic cells. This motility was inhibited by addition of anti-beta3 antibodies. Injection of the alphavbeta3-negative K1735C23 cells into syngeneic mice resulted in the generation of a metastatic variant (K1735C23PM) that neo expressed the alphavbeta3 complex, indicating that expression of alphavbeta3 is required for K1735 melanoma metastasis. Injection of highly metastatic K1735M2 cells in the presence of blocking antibody to beta3 reduced tumor size by approximately 80%. Treatment of the K1735M2 cells with a retroviral antisense beta3 construct significantly reduced their expression of alphavbeta3 and also reduced their motility on extracellular matrix ligands and their invasion through a reconstituted basement membrane. In contrast, when the K1735C23 cells were treated with a construct containing the full-length beta3 cDNA, their motility on extracellular matrix proteins and invasion of a reconstituted basement membrane were significantly increased. These results indicate that alphavbeta3 is required for migration and invasion of K1735 melanoma cells in vitro and primary tumor growth and metastasis in vivo.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21078267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Stimulation of motility of human renal cell carcinoma by SPARC/Osteonectin/BM-40 associated with type IV collagen. 与IV型胶原相关的SPARC/骨连接素/BM-40对人肾细胞癌运动的刺激作用

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024503

Y Kato, N Sakai, M Baba, S Kaneko, K Kondo, Y Kubota, M Yao, T Shuin, S Saito, S Koshika, T Kawase, Y Miyagi, I Aoki, Y Nagashima

引用次数: 19

Cyclosporin A upmodulates the alpha-subunit of the interleukin-2 receptor and the metastatic ability of murine B16F10 melanoma cells. 环孢素A上调白细胞介素-2受体α亚基和小鼠B16F10黑色素瘤细胞的转移能力。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024505

M D Boyano, A G de Galdeano, M D García-Vázquez, A Alvarez, M Luz Cañavate

{"title":"Cyclosporin A upmodulates the alpha-subunit of the interleukin-2 receptor and the metastatic ability of murine B16F10 melanoma cells.","authors":"M D Boyano, A G de Galdeano, M D García-Vázquez, A Alvarez, M Luz Cañavate","doi":"10.1159/000024505","DOIUrl":"https://doi.org/10.1159/000024505","url":null,"abstract":"In the present study, the effect of in vitro cyclosporin A (CsA) treatment on IL-2R expression and the metastatic behavior of B16F10 melanoma cells has been reported. CsA treatment was found to increase the percentage of B16F10 cells expressing the alpha-subunit of IL-2R on the cell surface and also at the mRNA level. Moreover, CsA treated B16F10 cells also express the beta-subunit of IL-2. In vivo experiments showed that CsA increases the affinity of B16F10 metastazing cells for the liver and decreases that for the lung. CsA modulated the expression of MHC class I and class II antigens, but no significant differences in the resistance of CsA-treated B16F10 cells to NK lysis were observed. Finally, proliferation of B16F10 cells in the presence of several doses of CsA did not vary and CsA increased the amount of IL-1beta mRNA expression. These results suggest that CsA, through the modulation of cytokines and MHC antigen expression on B16F10 cells, could have an effect upon the metastatic progression of the B16F10 melanoma.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 3","pages":"122-33"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A new bioassay using transient transfection for invasion-related gene analysis. 一种利用瞬时转染进行入侵相关基因分析的新生物测定方法。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024513

N Platet, M Garcia

{"title":"A new bioassay using transient transfection for invasion-related gene analysis.","authors":"N Platet, M Garcia","doi":"10.1159/000024513","DOIUrl":"https://doi.org/10.1159/000024513","url":null,"abstract":"To understand the mechanisms of tumor invasion and metastasis, model systems are required that isolate the individual steps of these complicated, multifaceted processes. We propose a new procedure to identify genes involved in cell invasion and/or motility that features the combined advantages of transient gene transfection and Matrigel invasion assays. Cancer cells were transiently cotransfected with two vectors expressing the gene of interest and luciferase, as a marker of transfected cells, and then assayed for Matrigel invasion. Luciferase cotransfection appeared to be a sensitive semiquantitative assay for transfected cells and was maximal throughout the invasion assay. The proposed transfection procedure, using calcium phosphate precipitation, did not affect cell invasiveness and allowed cellular coexpression of both genes. When applying this method, we found that transient expression of the unliganded and liganded human estrogen receptor alpha prevented invasiveness of MDA-MB-231 breast cancer cells. In conclusion, we propose rapid and versatile in vitro procedure for studying the effects of individual cloned genes on cellular processes, such as invasion and motility.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 4","pages":"198-208"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21496152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Mechanisms of downregulation of transfected E-cadherin cDNA during formation of invasive tumors in syngeneic mice. 同源小鼠侵袭性肿瘤形成过程中转染E-cadherin cDNA下调的机制。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024498

A Keirsebilck, L Van Hoorde, Y Gao, G De Bruyne, E Bruyneel, P Vermassen, M Mareel, F van Roy

{"title":"Mechanisms of downregulation of transfected E-cadherin cDNA during formation of invasive tumors in syngeneic mice.","authors":"A Keirsebilck, L Van Hoorde, Y Gao, G De Bruyne, E Bruyneel, P Vermassen, M Mareel, F van Roy","doi":"10.1159/000024498","DOIUrl":"https://doi.org/10.1159/000024498","url":null,"abstract":"Loss of E-cadherin expression has been observed both in experimental tumors and in human cancers and is related to invasiveness and poor differentiation. The E-cadherin-negative mouse mesenchymal tumor cell line MO4 was transfected with several plasmids expressing mouse E-cadherin cDNA. These plasmids differed from each other by the extent of E-cadherin-specific 3' untranslated region (UTR) sequences and by the use of different constitutive promoters. Transfectants were isolated that expressed functional E-cadherin in a homogeneous way. In syngeneic mice, such MO4-Ecad transfectants invariably produced malignant fibrosarcoma-like tumors, which were completely E-cadherin-negative at the protein level. Northern blotting revealed that E-cadherin mRNA expression was downregulated in some but not all MO4-Ecad tumors. Downregulation was caused by mRNA instability triggered by particular 3' UTR sequences. This in vivo downregulation of E-cadherin in malignant MO4-Ecad tumors turned out to be reversible and is likely to be mediated by host factors to be further identified.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 1","pages":"44-56"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21078271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Invasion strategies of the oral pathogen porphyromonas gingivalis: implications for cardiovascular disease. 口腔病原体牙龈卟啉单胞菌的侵袭策略:对心血管疾病的影响。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024499

R G Deshpande, M Khan, C A Genco

{"title":"Invasion strategies of the oral pathogen porphyromonas gingivalis: implications for cardiovascular disease.","authors":"R G Deshpande, M Khan, C A Genco","doi":"10.1159/000024499","DOIUrl":"https://doi.org/10.1159/000024499","url":null,"abstract":"Microorganisms have evolved a variety of mechanisms designed to evade detection and/or destruction by the host. Many pathogens evade host defenses by invading cells, thus providing the bacterium with an environment free of competing microorganisms. Adherence and invasion are active processes in which microorganisms often use host proteins and enzymes to gain entry into the cell, thus stimulating their own uptake. The investigation of invasion by the periopathogen Porphyromonas gingivalis is in its infancy in comparison with that of the enteric pathogens. However, recent studies with P. gingivalis have revealed that these organisms have developed invasion strategies and mechanisms similar to those of the enteric pathogens for both epithelial and endothelial cells. The study and elucidation of the mechanisms by which microorganisms such as P. gingivalis persist in chronic infection will provide valuable insight into the pathogenesis of P. gingivalis-mediated periodontal disease. The ability to multiply in and to activate endothelial cells may be one of the pathogenic mechanisms exerted by P. gingivalis that may explain the recently described association between this organism and cardiovascular disease.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 2","pages":"57-69"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024499","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21233470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96

Increased tumorigenicity and invasiveness of C6 rat glioma cells transfected with the human alpha-2,8 sialyltransferase cDNA. 转染人α -2,8唾液基转移酶cDNA的C6大鼠胶质瘤细胞的致瘤性和侵袭性增强

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024507

E Sottocornola, I Colombo, V Vergani, G Taraboletti, B Berra

{"title":"Increased tumorigenicity and invasiveness of C6 rat glioma cells transfected with the human alpha-2,8 sialyltransferase cDNA.","authors":"E Sottocornola, I Colombo, V Vergani, G Taraboletti, B Berra","doi":"10.1159/000024507","DOIUrl":"https://doi.org/10.1159/000024507","url":null,"abstract":"Gangliosides are thought to be involved in tumor cell proliferation, migration and invasiveness as so far demonstrated by the addition of exogenous gangliosides to the culture medium. To better understand the direct influence that alterations in ganglioside synthesis can exert on these functional aspects of cell biology, in the present study, we investigated the behaviour of C6 rat glioma cells after stable transfection with the human CMP-NeuAc:NeuAcalpha2-3Galbeta1-4GlcCer alpha2,8-sialyltransferase (SAT-II, EC 2.4.99.8) gene. The enzyme synthesizes ganglioside GD(3) by adding a sialic acid residue to ganglioside GM(3). Stable transfection of the constructs into C6 cells and expression of the human SAT-II gene were evaluated using PCR and RT-PCR amplification, respectively. Qualitative and quantitative analysis of the ganglioside profile was performed by conventional HP-TLC and identity of de novo synthesized species was assessed by TLC immunostaining. Results show that whereas C6 parental cells and C6 cells transfected with the empty expression vector synthesize, almost exclusively, ganglioside GM(3), de novo synthesis of GD(3) is clearly observed in clones expressing the alpha2,8-sialyltransferase. Subcutaneous grafting in athymic nude mice of cells expressing high levels of GD(3) induces tumors growing faster and more aggressively than controls. In in vitro assays, the same cells demonstrate increased proliferation rate, motility and invasiveness. Chemotaxis and chemoinvasion were assayed using the modified Boyden chamber. Data obtained suggest that endogenously neosynthesized GD(3) is able to modify proliferation rate, motility and invasion of C6 rat glioma cells, enhancing the features of malignancy of this tumor cell line.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 3","pages":"142-54"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Effects of EGF and TGF-alpha on invasion and proteinase expression of uterine cervical adenocarcinoma OMC-4 cells. EGF和tgf - α对子宫颈腺癌OMC-4细胞侵袭及蛋白酶表达的影响

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024510

M Ueda, H Fujii, K Yoshizawa, Y Terai, K Kumagai, K Ueki, M Ueki

{"title":"Effects of EGF and TGF-alpha on invasion and proteinase expression of uterine cervical adenocarcinoma OMC-4 cells.","authors":"M Ueda, H Fujii, K Yoshizawa, Y Terai, K Kumagai, K Ueki, M Ueki","doi":"10.1159/000024510","DOIUrl":"https://doi.org/10.1159/000024510","url":null,"abstract":"Uterine cervical adenocarcinoma typically is an aggressive neoplasm with a propensity for early invasion and dissemination; however, the regulatory mechanism of invasive activity of cervical adenocarcinoma cells has not been fully understood. In this study, biological effects of epidermal growth factor (EGF) and transforming growth factor (TGF)-alpha on invasion and proteinase expression of human cervical adenocarcinoma OMC-4 cells were investigated. Tumor cell migration along a gradient of substratum-bound fibronectin and invasion into the reconstituted basement membrane were stimulated by 0.1-10 nM EGF and TGF-alpha in a concentration-dependent manner. Their effects on tumor cell migration were also confirmed by wound assay. The zymography of tumor-conditioned medium showed that the treatment of OMC-4 cells with EGF and TGF-alpha resulted in the increase of matrix metalloproteinase (MMP)-2 and urokinase-type plasminogen activator (uPA). Matrilysin (MMP-7), also secreted by OMC-4 cells, was not affected by these growth factors. These results suggest that EGF and TGF-alpha act as positive regulators on the invasion of cervical adenocarcinoma cells, which may be associated with their stimulatory effects on tumor cell motility and the induction of type IV collagenase and uPA secreted by tumor cells.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 4","pages":"176-83"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024510","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21496232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Establishment of high and low metastasis cell lines derived from a human tongue squamous cell carcinoma. 人舌鳞状细胞癌高、低转移细胞系的建立。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024515

S Nakayama, A Sasaki, H Mese, R E Alcalde, T Matsumura

引用次数: 49

Maximum effect of urokinase plasminogen activator inhibitors in the control of invasion and metastasis of rat mammary cancer. 尿激酶纤溶酶原激活物抑制剂在控制大鼠乳腺癌侵袭转移中的最大作用。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024518

D M Evans, K D Sloan-Stakleff

引用次数: 18