Invasion & metastasis最新文献_第2页

Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in non-small-cell lung cancer. 基质金属蛋白酶及基质金属蛋白酶组织抑制剂在非小细胞肺癌中的表达。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024506

M Suzuki, T Iizasa, T Fujisawa, M Baba, Y Yamaguchi, H Kimura, H Suzuki

{"title":"Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in non-small-cell lung cancer.","authors":"M Suzuki, T Iizasa, T Fujisawa, M Baba, Y Yamaguchi, H Kimura, H Suzuki","doi":"10.1159/000024506","DOIUrl":"https://doi.org/10.1159/000024506","url":null,"abstract":"Expression of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of metalloproteinases (TIMP)-1, and TIMP-2 was studied in non-small-cell lung cancer (NSCLC). Activity of MMP-2 and MMP-9 by gelatin zymography and expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 mRNAs were examined in 11 lung cancer cell lines which included six small-cell lung cancer (SCLC) cell lines. Localization of MMP-2, MMP-9, TIMP-1, and TIMP-2 was examined by immunohistochemistry in 43 resected NSCLC (22 adenocarcinomas, 17 squamous cell carcinomas, 4 large cell carcinomas) using specific anti-human monoclonal antibodies. Expression of MMP-2 mRNA was detected in 5 (100%), MMP-9 in 1 (20%), TIMP-1 in 4 (80%), and TIMP-2 in 5 (100%) of 5 NSCLC cell lines examined. MMP-2 gelatinolytic activity also was detected in all five NSCLC cell lines, whereas MMP-9 activity was detected in only one cell line. In 43 patients, MMP-2, MMP-9, TIMP-1, and TIMP-2 immunoreactivity was demonstrated in 19 (44%), 9 (21%), 15 (35%), and 29 (67%) excised tumors, respectively. All stromal fibroblasts in tumor samples stained positive for MMP-2. There was a correlation between TIMP-2 immunoreactivity and disease stage (42% stage I versus 88% stages II, III, and IV) (p = 0.0024). Both cancer cell lines and NSCLC tumor samples frequently expressed MMP-2, MMP-9, TIMP-1, and TIMP-2; MMP-2 in particular was highly expressed in malignant cells and surrounding fibroblasts. These findings suggest that MMP-2 plays a more important role in invasion of NSCLC than MMP-9 and that TIMP-2 may have clinical relevance in NSCLC.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 3","pages":"134-41"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Expression of N-acetyl galactosaminylated and sialylated glycans by metastases arising from primary breast cancer. n -乙酰半乳糖氨基化和唾液化聚糖在原发性乳腺癌转移中的表达。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024504

S A Brooks, A J Leathem

引用次数: 28

Effects of all-trans-retinoic acid incorporated into low-density lipoprotein on invasive properties of multidrug-resistant MCF-7 spheroids. 低密度脂蛋白掺入全反式维甲酸对多药耐药MCF-7球体侵袭特性的影响

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024516

M Affoué, M G Akeli, N Gueddari, J C Jardillier, C Madoulet

{"title":"Effects of all-trans-retinoic acid incorporated into low-density lipoprotein on invasive properties of multidrug-resistant MCF-7 spheroids.","authors":"M Affoué, M G Akeli, N Gueddari, J C Jardillier, C Madoulet","doi":"10.1159/000024516","DOIUrl":"https://doi.org/10.1159/000024516","url":null,"abstract":"Cultured cells grown as spheroids provide an in vitro model that is closer to an in vivo tumour than conventional monolayer techniques. Previous work from our laboratory has demonstrated that spheroids formed from multidrug-resistant MCF-7 cells exhibit invasive characteristics which were not present in their sensitive counterparts. The treatment of these spheroids by all-trans-retinoic acid (ATRA), a potent inducer of in vitro and in vivo differentiation, decreases their proteolytic activity and ability to invade Matrigel-coated filters. The efficiency of ATRA is enhanced by its incorporation into low-density lipoprotein (LDL) (LDL-ATRA). Indeed, invasion through a reconstituted basement membrane was reduced by 73% with 10(-6) M ATRA and 3 x 10(-8) M LDL-ATRA. Furthermore, inhibition of invasion was correlated with a decrease in several factors: (1) secreted matrix metalloproteinase-9 and enzymes degrading type IV collagen and Matrigel films, and (2) tissue plasminogen activator. The results observed were found with a concentration of LDL-ATRA 30 times lower than that of ATRA. This could be due to the protective effect of LDL and to a better targeting of cancer cells through their LDL receptors. LDL-ATRA may therefore represent a new and potent inhibitor of invasion that could be developed for clinical trials.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 5-6","pages":"229-39"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024516","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21580878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Elaboration of matrix metalloproteinase inhibitors by human skin in organ culture and by skin cells in monolayer culture: relationship to invasion. 人皮肤在器官培养和皮肤细胞在单层培养中制备基质金属蛋白酶抑制剂:与侵袭的关系。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024496

Y Chi, M E Zeigler, J Walker, P Perone, S C Datta, J Varani

{"title":"Elaboration of matrix metalloproteinase inhibitors by human skin in organ culture and by skin cells in monolayer culture: relationship to invasion.","authors":"Y Chi, M E Zeigler, J Walker, P Perone, S C Datta, J Varani","doi":"10.1159/000024496","DOIUrl":"https://doi.org/10.1159/000024496","url":null,"abstract":"Functional and immunochemical approaches were used to assess matrix metalloproteinase (MMP) inhibitors, e.g., tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2), in organ cultures of normal human skin maintained under growth factor free conditions or in medium supplemented with a combination of growth factors including epidermal growth factor, insulin, and pituitary extract. It has previously been shown that under growth factor free conditions, normal skin structure and function are maintained for several days, while in the presence of these exogenous growth factors, the epithelial cells invade the stroma [Invasion and Metastasis 1993;13:225-233]. TIMP-1 was detected in equivalent amounts in organ culture fluids under both conditions. TIMP-2 was not detected under either condition. Normal epidermal keratinocytes, normal dermal fibroblasts, and three different epithelial tumor cell lines were also examined for MMP inhibitor expression. Keratinocytes and fibroblasts produced high levels of both TIMP-1 and TIMP-2, but in neither cell type was there a significant difference between growth factor free and growth factor containing conditions. In contrast, the three epithelial tumor cell lines produced low to undetectable levels of both TIMP-1 and TIMP-2. These data suggest that acquisition of local invasive capacity is not dependent on a reduction in MMP inhibitor expression. A reduction in MMP inhibitors may accompany the transition from invasive to metastatic tumors.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 1","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21078269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Invasive potential and substrate dependence of attachment in the dunning R-3327 rat prostate adenocarcinoma model. dunning R-3327大鼠前列腺癌模型中附着的侵袭电位和底物依赖性。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024509

C D Donald, D E Montgomery, N Emmett, D B Cooke

{"title":"Invasive potential and substrate dependence of attachment in the dunning R-3327 rat prostate adenocarcinoma model.","authors":"C D Donald, D E Montgomery, N Emmett, D B Cooke","doi":"10.1159/000024509","DOIUrl":"https://doi.org/10.1159/000024509","url":null,"abstract":"Cancer cell attachment to and invasion of the extracellular matrix has been associated with the metastatic potential of cell lines of the Dunning R-3327 rat prostatic adenocarcinoma model. We investigated the cell-matrix interactions of prostate tumor cells by comparing the invasive ability through reconstructed extracellular matrix and attachment upon EHS NATRIX (natural extracellular matrix), fibronectin, laminin, and collagen Type IV. We observed a correlation between metastatic potential and substrate dependence of attachment in prostate cancer cells. Nonmetastatic AT-1 cells possessed a higher adhesive potential to extracellular matrix components than the highly metastatic cells (ML, MLL and AT-3). It was also found that the invasive potential of the three highly metastatic cell lines was significantly higher than that of the nonmetastatic cell line. Here, it is reported that the ability to traverse a matrigel matrix correlates with their metastatic potential. These observations suggest that the extracellular matrix components are highly involved in influencing prostate cancer cell activities. In addition, we investigated the effects of two differentiation agents, retinoic acid (RA) and difluoromethylornithine (DFMO), on the adhesive and invasive profiles of the tumor cells. After treatment with both agents, adhesion was increased to levels not different from nonmetastatic cells. Furthermore, the ability of highly metastatic cells to traverse a matrigel barrier was significantly reduced after treatment with both differentiation agents. These results suggest that RA and DFMO are capable in reversing the metastatic potential of prostate cancer cells in vitro and may give a possible insight into their role as potential therapeutic agents in vivo.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 4","pages":"165-75"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21496230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

A model system to quantitate tumor burden in locoregional lymph nodes during cancer spread. 肿瘤扩散过程中局部淋巴结肿瘤负荷的定量模型系统。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024512

A Moore, N Sergeyev, S Bredow, R Weissleder

引用次数: 30

Differential expression of alphav integrins in K1735 melanoma cells. α - v整合素在K1735黑色素瘤细胞中的差异表达

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024494

X Li, B Chen, S D Blystone, K P McHugh, F P Ross, D M Ramos

{"title":"Differential expression of alphav integrins in K1735 melanoma cells.","authors":"X Li, B Chen, S D Blystone, K P McHugh, F P Ross, D M Ramos","doi":"10.1159/000024494","DOIUrl":"https://doi.org/10.1159/000024494","url":null,"abstract":"Tumor cell adherence to and migration on the extracellular matrix is an important aspect of cancer progression. This interaction with the extracellular matrix is mediated primarily through the integrin class of cell adhesion molecules. We identified a restricted expression of alphavbeta3 in highly metastatic K1735M2 and of alphavbeta5 in poorly metastatic K1735C23 murine melanoma cells. The highly metastatic cells were ten times more motile on vitronectin and fibronectin and approximately three times more invasive through a reconstituted basement membrane than the poorly metastatic cells. This motility was inhibited by addition of anti-beta3 antibodies. Injection of the alphavbeta3-negative K1735C23 cells into syngeneic mice resulted in the generation of a metastatic variant (K1735C23PM) that neo expressed the alphavbeta3 complex, indicating that expression of alphavbeta3 is required for K1735 melanoma metastasis. Injection of highly metastatic K1735M2 cells in the presence of blocking antibody to beta3 reduced tumor size by approximately 80%. Treatment of the K1735M2 cells with a retroviral antisense beta3 construct significantly reduced their expression of alphavbeta3 and also reduced their motility on extracellular matrix ligands and their invasion through a reconstituted basement membrane. In contrast, when the K1735C23 cells were treated with a construct containing the full-length beta3 cDNA, their motility on extracellular matrix proteins and invasion of a reconstituted basement membrane were significantly increased. These results indicate that alphavbeta3 is required for migration and invasion of K1735 melanoma cells in vitro and primary tumor growth and metastasis in vivo.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21078267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Stimulation of motility of human renal cell carcinoma by SPARC/Osteonectin/BM-40 associated with type IV collagen. 与IV型胶原相关的SPARC/骨连接素/BM-40对人肾细胞癌运动的刺激作用

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024503

Y Kato, N Sakai, M Baba, S Kaneko, K Kondo, Y Kubota, M Yao, T Shuin, S Saito, S Koshika, T Kawase, Y Miyagi, I Aoki, Y Nagashima

引用次数: 19

Cyclosporin A upmodulates the alpha-subunit of the interleukin-2 receptor and the metastatic ability of murine B16F10 melanoma cells. 环孢素A上调白细胞介素-2受体α亚基和小鼠B16F10黑色素瘤细胞的转移能力。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024505

M D Boyano, A G de Galdeano, M D García-Vázquez, A Alvarez, M Luz Cañavate

{"title":"Cyclosporin A upmodulates the alpha-subunit of the interleukin-2 receptor and the metastatic ability of murine B16F10 melanoma cells.","authors":"M D Boyano, A G de Galdeano, M D García-Vázquez, A Alvarez, M Luz Cañavate","doi":"10.1159/000024505","DOIUrl":"https://doi.org/10.1159/000024505","url":null,"abstract":"In the present study, the effect of in vitro cyclosporin A (CsA) treatment on IL-2R expression and the metastatic behavior of B16F10 melanoma cells has been reported. CsA treatment was found to increase the percentage of B16F10 cells expressing the alpha-subunit of IL-2R on the cell surface and also at the mRNA level. Moreover, CsA treated B16F10 cells also express the beta-subunit of IL-2. In vivo experiments showed that CsA increases the affinity of B16F10 metastazing cells for the liver and decreases that for the lung. CsA modulated the expression of MHC class I and class II antigens, but no significant differences in the resistance of CsA-treated B16F10 cells to NK lysis were observed. Finally, proliferation of B16F10 cells in the presence of several doses of CsA did not vary and CsA increased the amount of IL-1beta mRNA expression. These results suggest that CsA, through the modulation of cytokines and MHC antigen expression on B16F10 cells, could have an effect upon the metastatic progression of the B16F10 melanoma.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 3","pages":"122-33"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A new bioassay using transient transfection for invasion-related gene analysis. 一种利用瞬时转染进行入侵相关基因分析的新生物测定方法。

Invasion & metastasis Pub Date : 1998-01-01 DOI: 10.1159/000024513

N Platet, M Garcia

{"title":"A new bioassay using transient transfection for invasion-related gene analysis.","authors":"N Platet, M Garcia","doi":"10.1159/000024513","DOIUrl":"https://doi.org/10.1159/000024513","url":null,"abstract":"To understand the mechanisms of tumor invasion and metastasis, model systems are required that isolate the individual steps of these complicated, multifaceted processes. We propose a new procedure to identify genes involved in cell invasion and/or motility that features the combined advantages of transient gene transfection and Matrigel invasion assays. Cancer cells were transiently cotransfected with two vectors expressing the gene of interest and luciferase, as a marker of transfected cells, and then assayed for Matrigel invasion. Luciferase cotransfection appeared to be a sensitive semiquantitative assay for transfected cells and was maximal throughout the invasion assay. The proposed transfection procedure, using calcium phosphate precipitation, did not affect cell invasiveness and allowed cellular coexpression of both genes. When applying this method, we found that transient expression of the unliganded and liganded human estrogen receptor alpha prevented invasiveness of MDA-MB-231 breast cancer cells. In conclusion, we propose rapid and versatile in vitro procedure for studying the effects of individual cloned genes on cellular processes, such as invasion and motility.","PeriodicalId":14452,"journal":{"name":"Invasion & metastasis","volume":"18 4","pages":"198-208"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000024513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21496152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12